Rh4AG021000

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0001095
Physical Location & Seq
Forward (+)
3950712 .. 3951339
628 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG021000.1

Sequence Viewer

Length: 423 bp
ATGGCGGGAAATTCAAATGAAAATAAAGGCGCGCCATCCCTTATCAAAGCACTTTGCTCTTGTGGCCGGGCGTGGAATCTGATGGAGCATTCGAAGCCGAAAATTCAAACCTCTGATTCTCAGAACCTCCTGAGTCCTGATCTGGGCAGTTGGTTGGCAAGCTACCGGTATGAATCACCTCTAATGAATTCCAACTCTTTTCGCAATGAATTCGATGGACGCGAGCGAAAGAGAAAAAGGACTGATGTGGTTGTTGCTTTGAGCTTGAGGAAGAAGAGCCTGGGCGATTTTGGCCCCCATTGTAAGAGTTGTGACGAGGTCTCTTATTGCAGCGAGCTTCAGTATGCCCTTTTAGATCACAAAAATCACATTGATACATCTTCTTGTTCTTTTTGTTACAACGAGACTGGCAATGACCGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

140

Amino Acids

15.76

Weight (kDa)

8.16

Isoelectric Point (pI)

44.34

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 32, 222
AciI CCGC 1 cut(s) 5
AcoI YGGCCR 1 cut(s) 64
AcsI RAATTY 4 cut(s) 10, 102, 187, 209
AcuI CTGAAG 1 cut(s) 323
AfiI CCNNNNNNNGG 1 cut(s) 143
AgeI ACCGGT 1 cut(s) 165
AgsI TTSAA 2 cut(s) 15, 107
AjnI CCWGG 1 cut(s) 279
AluBI AGCT 3 cut(s) 162, 264, 337
AluI AGCT 3 cut(s) 162, 264, 337
Alw26I GTCTC 2 cut(s) 325, 398
AoxI GGCC 2 cut(s) 64, 292
ApeKI GCWGC 1 cut(s) 330
ApoI RAATTY 4 cut(s) 10, 102, 187, 209
AscI GGCGCGCC 1 cut(s) 30
AsiGI ACCGGT 1 cut(s) 165
AspLEI GCGC 2 cut(s) 32, 34
AspS9I GGNCC 1 cut(s) 293
AsuC2I CCSGG 1 cut(s) 68
AsuHPI GGTGA 1 cut(s) 168
AsuII TTCGAA 1 cut(s) 92
BbvI GCAGC 1 cut(s) 342
BccI CCATC 3 cut(s) 43, 76, 209
BcgI CGANNNNNNTGC 2 cut(s) 193, 227
BciT130I CCWGG 1 cut(s) 281
BcnI CCSGG 1 cut(s) 68
BcoDI GTCTC 2 cut(s) 325, 398
BisI GCNGC 1 cut(s) 331
BlsI GCNGC 1 cut(s) 332
Bme1390I CCNGG 2 cut(s) 68, 281
BmgT120I GGNCC 1 cut(s) 293
BmiI GGNNCC 1 cut(s) 295
BmrFI CCNGG 2 cut(s) 68, 281
Bpu14I TTCGAA 1 cut(s) 92
BpuEI CTTGAG 1 cut(s) 286
BpuMI CCSGG 1 cut(s) 68
BsaI GGTCTC 1 cut(s) 325
BsaJI CCNNGG 1 cut(s) 280
BsaWI WCCGGW 1 cut(s) 165
Bsc4I CCNNNNNNNGG 1 cut(s) 143
Bse118I RCCGGY 1 cut(s) 165
Bse1I ACTGG 1 cut(s) 412
Bse3DI GCAATG 2 cut(s) 211, 418
BseBI CCWGG 1 cut(s) 281
BseDI CCNNGG 1 cut(s) 280
BseGI GGATG 1 cut(s) 35
BseLI CCNNNNNNNGG 1 cut(s) 143
BseMI GCAATG 2 cut(s) 211, 418
BseMII CTCAG 2 cut(s) 122, 134
BseNI ACTGG 1 cut(s) 412
BsePI GCGCGC 1 cut(s) 30
BseXI GCAGC 1 cut(s) 342
Bsh1236I CGCG 2 cut(s) 32, 222
BshFI GGCC 2 cut(s) 66, 294
BshTI ACCGGT 1 cut(s) 165
BsiSI CCGG 2 cut(s) 67, 166
BslI CCNNNNNNNGG 1 cut(s) 143
BsmAI GTCTC 2 cut(s) 325, 398
BsmI GAATGC 1 cut(s) 88
BsnI GGCC 2 cut(s) 66, 294
Bso31I GGTCTC 1 cut(s) 325
Bsp119I TTCGAA 1 cut(s) 92
Bsp143I GATC 2 cut(s) 139, 355
BspACI CCGC 1 cut(s) 5
BspANI GGCC 2 cut(s) 66, 294
BspCNI CTCAG 2 cut(s) 123, 133
BspFNI CGCG 2 cut(s) 32, 222
BspLI GGNNCC 1 cut(s) 295
BspQI GCTCTTC 1 cut(s) 269
BspT104I TTCGAA 1 cut(s) 92
BspTNI GGTCTC 1 cut(s) 325
BsrDI GCAATG 2 cut(s) 211, 418
BsrFI RCCGGY 1 cut(s) 165
BsrI ACTGG 1 cut(s) 412
BssAI RCCGGY 1 cut(s) 165
BssECI CCNNGG 1 cut(s) 280
BssHII GCGCGC 1 cut(s) 30
BssMI GATC 2 cut(s) 139, 355
Bst2UI CCWGG 1 cut(s) 281
Bst4CI ACNGT 1 cut(s) 419
Bst6I CTCTTC 1 cut(s) 269
BstBI TTCGAA 1 cut(s) 92
BstC8I GCNNGC 4 cut(s) 32, 160, 224, 335
BstDEI CTNAG 2 cut(s) 120, 131
BstF5I GGATG 1 cut(s) 35
BstFNI CGCG 2 cut(s) 32, 222
BstHHI GCGC 2 cut(s) 32, 34
BstKTI GATC 2 cut(s) 142, 358
BstMAI GTCTC 2 cut(s) 325, 398
BstMBI GATC 2 cut(s) 139, 355
BstMWI GCNNNNNNNGC 3 cut(s) 63, 94, 291
BstNI CCWGG 1 cut(s) 281
BstSCI CCNGG 2 cut(s) 66, 279
BstUI CGCG 2 cut(s) 32, 222
BstV1I GCAGC 1 cut(s) 342
BsuRI GGCC 2 cut(s) 66, 294
BtsCI GGATG 1 cut(s) 35
Cac8I GCNNGC 4 cut(s) 32, 160, 224, 335
CfoI GCGC 2 cut(s) 32, 34
Cfr10I RCCGGY 1 cut(s) 165
Cfr13I GGNCC 1 cut(s) 293
CseI GACGC 1 cut(s) 228
CspAI ACCGGT 1 cut(s) 165
CviJI RGCY 7 cut(s) 66, 97, 162, 264, 279, 294, 337
CviKI_1 RGCY 7 cut(s) 66, 97, 162, 264, 279, 294, 337
DdeI CTNAG 2 cut(s) 120, 131
DpnI GATC 2 cut(s) 141, 357
DpnII GATC 2 cut(s) 139, 355
EaeI YGGCCR 1 cut(s) 64
Eam1104I CTCTTC 1 cut(s) 269
EarI CTCTTC 1 cut(s) 269
Eco31I GGTCTC 1 cut(s) 325
Eco57I CTGAAG 1 cut(s) 323
EcoRI GAATTC 2 cut(s) 187, 209
EcoRII CCWGG 1 cut(s) 279
FaiI YATR 2 cut(s) 171, 345
Fnu4HI GCNGC 1 cut(s) 331
FokI GGATG 1 cut(s) 22
Fsp4HI GCNGC 1 cut(s) 331
GlaI GCGC 2 cut(s) 31, 33
GluI GCNGC 1 cut(s) 331
HaeIII GGCC 2 cut(s) 66, 294
HapII CCGG 2 cut(s) 67, 166
HgaI GACGC 1 cut(s) 228
HhaI GCGC 2 cut(s) 32, 34
Hin6I GCGC 2 cut(s) 30, 32
HinP1I GCGC 2 cut(s) 30, 32
HinfI GANTC 4 cut(s) 76, 116, 133, 173
HpaII CCGG 2 cut(s) 67, 166
HphI GGTGA 1 cut(s) 168
Hpy188I TCNGA 3 cut(s) 81, 115, 123
Hpy188III TCNNGA 2 cut(s) 130, 137
HpyCH4III ACNGT 1 cut(s) 419
HpyCH4V TGCA 1 cut(s) 330
HpyF10VI GCNNNNNNNGC 3 cut(s) 63, 94, 291
HpyF3I CTNAG 2 cut(s) 120, 131
HspAI GCGC 2 cut(s) 30, 32
Kzo9I GATC 2 cut(s) 139, 355
LguI GCTCTTC 1 cut(s) 269
LmnI GCTCC 1 cut(s) 85
LpnPI CCDG 8 cut(s) 80, 128, 143, 150, 179, 266, 293, 393
Lsp1109I GCAGC 1 cut(s) 342
MaeIII GTNAC 2 cut(s) 311, 395
MalI GATC 2 cut(s) 141, 357
MboI GATC 2 cut(s) 139, 355
MboII GAAGA 3 cut(s) 283, 286, 372
MluCI AATT 4 cut(s) 10, 102, 187, 209
MlyI GAGTC 1 cut(s) 142
MmeI TCCRAC 1 cut(s) 216
MnlI CCTC 5 cut(s) 121, 137, 189, 261, 310
MspI CCGG 2 cut(s) 67, 166
MspR9I CCNGG 2 cut(s) 68, 281
Mva1269I GAATGC 1 cut(s) 88
MvaI CCWGG 1 cut(s) 281
MvnI CGCG 2 cut(s) 32, 222
MwoI GCNNNNNNNGC 3 cut(s) 63, 94, 291
NciI CCSGG 1 cut(s) 68
NdeII GATC 2 cut(s) 139, 355
NlaIV GGNNCC 1 cut(s) 295
NmuCI GTSAC 1 cut(s) 311
NspV TTCGAA 1 cut(s) 92
PalAI GGCGCGCC 1 cut(s) 30
PauI GCGCGC 1 cut(s) 30
PciSI GCTCTTC 1 cut(s) 269
PcsI WCGNNNNNNNCGW 1 cut(s) 219
PctI GAATGC 1 cut(s) 88
PfeI GAWTC 3 cut(s) 76, 116, 173
PflFI GACNNNGTC 1 cut(s) 317
PinAI ACCGGT 1 cut(s) 165
PkrI GCNGC 1 cut(s) 332
PleI GAGTC 1 cut(s) 141
PpsI GAGTC 1 cut(s) 141
Psp6I CCWGG 1 cut(s) 279
PspGI CCWGG 1 cut(s) 279
PspN4I GGNNCC 1 cut(s) 295
PspPI GGNCC 1 cut(s) 293
PsyI GACNNNGTC 1 cut(s) 317
PteI GCGCGC 1 cut(s) 30
SapI GCTCTTC 1 cut(s) 269
SatI GCNGC 1 cut(s) 331
Sau3AI GATC 2 cut(s) 139, 355
Sau96I GGNCC 1 cut(s) 293
SchI GAGTC 1 cut(s) 142
ScrFI CCNGG 2 cut(s) 68, 281
SetI ASST 7 cut(s) 113, 129, 164, 181, 266, 321, 339
SfuI TTCGAA 1 cut(s) 92
SgsI GGCGCGCC 1 cut(s) 30
SmlI CTYRAG 1 cut(s) 265
SmoI CTYRAG 1 cut(s) 265
Sse9I AATT 4 cut(s) 10, 102, 187, 209
SsiI CCGC 1 cut(s) 5
StyD4I CCNGG 2 cut(s) 66, 279
TaaI ACNGT 1 cut(s) 419
TaqI TCGA 2 cut(s) 92, 213
TasI AATT 4 cut(s) 10, 102, 187, 209
TfiI GAWTC 3 cut(s) 76, 116, 173
TseFI GTSAC 1 cut(s) 311
TseI GCWGC 1 cut(s) 330
Tsp45I GTSAC 1 cut(s) 311
TspDTI ATGAA 4 cut(s) 33, 186, 200, 222
Tth111I GACNNNGTC 1 cut(s) 317
XapI RAATTY 4 cut(s) 10, 102, 187, 209
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.