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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AasI | GACNNNNNNGTC | 1 cut(s) | 169 |
| AccB1I | GGYRCC | 1 cut(s) | 19 |
| AclWI | GGATC | 1 cut(s) | 188 |
| AcuI | CTGAAG | 1 cut(s) | 239 |
| AfaI | GTAC | 1 cut(s) | 111 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 29, 215 |
| AgsI | TTSAA | 2 cut(s) | 233, 350 |
| AluBI | AGCT | 2 cut(s) | 150, 290 |
| AluI | AGCT | 2 cut(s) | 150, 290 |
| AlwI | GGATC | 1 cut(s) | 188 |
| AoxI | GGCC | 1 cut(s) | 206 |
| AsuC2I | CCSGG | 1 cut(s) | 24 |
| AsuHPI | GGTGA | 1 cut(s) | 86 |
| BanI | GGYRCC | 1 cut(s) | 19 |
| BccI | CCATC | 1 cut(s) | 254 |
| BcnI | CCSGG | 1 cut(s) | 24 |
| BfaI | CTAG | 2 cut(s) | 116, 291 |
| BfmI | CTRYAG | 1 cut(s) | 282 |
| BglII | AGATCT | 1 cut(s) | 374 |
| Bme1390I | CCNGG | 1 cut(s) | 24 |
| BmiI | GGNNCC | 1 cut(s) | 21 |
| BmrFI | CCNGG | 1 cut(s) | 24 |
| BpuMI | CCSGG | 1 cut(s) | 24 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 267, 297 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 29, 215 |
| Bse118I | RCCGGY | 1 cut(s) | 77 |
| BseGI | GGATG | 1 cut(s) | 265 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 29, 215 |
| BseMII | CTCAG | 1 cut(s) | 258 |
| BshFI | GGCC | 1 cut(s) | 208 |
| BshNI | GGYRCC | 1 cut(s) | 19 |
| BsiSI | CCGG | 2 cut(s) | 23, 78 |
| BslFI | GGGAC | 1 cut(s) | 46 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 29, 215 |
| BsmFI | GGGAC | 1 cut(s) | 46 |
| BsnI | GGCC | 1 cut(s) | 208 |
| Bsp143I | GATC | 3 cut(s) | 193, 342, 374 |
| BspANI | GGCC | 1 cut(s) | 208 |
| BspCNI | CTCAG | 1 cut(s) | 259 |
| BspLI | GGNNCC | 1 cut(s) | 21 |
| BspPI | GGATC | 1 cut(s) | 188 |
| BspT107I | GGYRCC | 1 cut(s) | 19 |
| BsrFI | RCCGGY | 1 cut(s) | 77 |
| BssAI | RCCGGY | 1 cut(s) | 77 |
| BssMI | GATC | 3 cut(s) | 193, 342, 374 |
| Bst4CI | ACNGT | 2 cut(s) | 49, 283 |
| BstDEI | CTNAG | 1 cut(s) | 267 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 213 |
| BstF5I | GGATG | 1 cut(s) | 265 |
| BstKTI | GATC | 3 cut(s) | 196, 345, 377 |
| BstMBI | GATC | 3 cut(s) | 193, 342, 374 |
| BstSCI | CCNGG | 1 cut(s) | 22 |
| BstSFI | CTRYAG | 1 cut(s) | 282 |
| BstX2I | RGATCY | 1 cut(s) | 374 |
| BstYI | RGATCY | 1 cut(s) | 374 |
| BsuRI | GGCC | 1 cut(s) | 208 |
| BtsCI | GGATG | 1 cut(s) | 265 |
| Cfr10I | RCCGGY | 1 cut(s) | 77 |
| Csp6I | GTAC | 1 cut(s) | 110 |
| CviJI | RGCY | 4 cut(s) | 150, 208, 245, 290 |
| CviKI_1 | RGCY | 4 cut(s) | 150, 208, 245, 290 |
| CviQI | GTAC | 1 cut(s) | 110 |
| DdeI | CTNAG | 1 cut(s) | 267 |
| DpnI | GATC | 3 cut(s) | 195, 344, 376 |
| DpnII | GATC | 3 cut(s) | 193, 342, 374 |
| DrdI | GACNNNNNNGTC | 1 cut(s) | 169 |
| DseDI | GACNNNNNNGTC | 1 cut(s) | 169 |
| Eco57I | CTGAAG | 1 cut(s) | 239 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 213 |
| EcoT22I | ATGCAT | 1 cut(s) | 322 |
| FaiI | YATR | 6 cut(s) | 102, 155, 311, 318, 322, 326 |
| FalI | AAGNNNNNCTT | 1 cut(s) | 361 |
| FaqI | GGGAC | 1 cut(s) | 46 |
| FokI | GGATG | 1 cut(s) | 272 |
| FspBI | CTAG | 2 cut(s) | 116, 291 |
| HaeIII | GGCC | 1 cut(s) | 208 |
| HapII | CCGG | 2 cut(s) | 23, 78 |
| HpaII | CCGG | 2 cut(s) | 23, 78 |
| HphI | GGTGA | 1 cut(s) | 86 |
| Hpy166II | GTNNAC | 1 cut(s) | 45 |
| Hpy188III | TCNNGA | 3 cut(s) | 116, 233, 257 |
| Hpy8I | GTNNAC | 1 cut(s) | 45 |
| HpyCH4III | ACNGT | 2 cut(s) | 49, 283 |
| HpyCH4V | TGCA | 2 cut(s) | 213, 320 |
| HpyF3I | CTNAG | 1 cut(s) | 267 |
| Kzo9I | GATC | 3 cut(s) | 193, 342, 374 |
| LpnPI | CCDG | 5 cut(s) | 36, 91, 117, 145, 242 |
| MaeI | CTAG | 2 cut(s) | 116, 291 |
| MaeIII | GTNAC | 3 cut(s) | 262, 295, 364 |
| MalI | GATC | 3 cut(s) | 195, 344, 376 |
| MboI | GATC | 3 cut(s) | 193, 342, 374 |
| MfeI | CAATTG | 1 cut(s) | 139 |
| MflI | RGATCY | 1 cut(s) | 374 |
| MluCI | AATT | 2 cut(s) | 139, 239 |
| MnlI | CCTC | 2 cut(s) | 172, 352 |
| Mph1103I | ATGCAT | 1 cut(s) | 322 |
| MseI | TTAA | 1 cut(s) | 379 |
| MslI | CAYNNNNRTG | 2 cut(s) | 99, 329 |
| MspI | CCGG | 2 cut(s) | 23, 78 |
| MspR9I | CCNGG | 1 cut(s) | 24 |
| MunI | CAATTG | 1 cut(s) | 139 |
| NciI | CCSGG | 1 cut(s) | 24 |
| NdeII | GATC | 3 cut(s) | 193, 342, 374 |
| NlaIV | GGNNCC | 1 cut(s) | 21 |
| NmuCI | GTSAC | 1 cut(s) | 262 |
| NsiI | ATGCAT | 1 cut(s) | 322 |
| PspN4I | GGNNCC | 1 cut(s) | 21 |
| PsuI | RGATCY | 1 cut(s) | 374 |
| RsaI | GTAC | 1 cut(s) | 111 |
| RsaNI | GTAC | 1 cut(s) | 110 |
| RseI | CAYNNNNRTG | 2 cut(s) | 99, 329 |
| SaqAI | TTAA | 1 cut(s) | 379 |
| Sau3AI | GATC | 3 cut(s) | 193, 342, 374 |
| ScrFI | CCNGG | 1 cut(s) | 24 |
| SetI | ASST | 3 cut(s) | 99, 152, 292 |
| SfcI | CTRYAG | 1 cut(s) | 282 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 99, 329 |
| Sse9I | AATT | 2 cut(s) | 139, 239 |
| SspMI | CTAG | 2 cut(s) | 116, 291 |
| StyD4I | CCNGG | 1 cut(s) | 22 |
| TaaI | ACNGT | 2 cut(s) | 49, 283 |
| TasI | AATT | 2 cut(s) | 139, 239 |
| TatI | WGTACW | 1 cut(s) | 109 |
| Tru1I | TTAA | 1 cut(s) | 379 |
| Tru9I | TTAA | 1 cut(s) | 379 |
| TseFI | GTSAC | 1 cut(s) | 262 |
| Tsp45I | GTSAC | 1 cut(s) | 262 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 213 |
| XbaI | TCTAGA | 1 cut(s) | 115 |
| XspI | CTAG | 2 cut(s) | 116, 291 |
| Zsp2I | ATGCAT | 1 cut(s) | 322 |