Rw4G000230

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0001095
Physical Location & Seq
Forward (+)
532347 .. 533355
1009 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G000230.1

Sequence Viewer

Length: 825 bp
ATGCTTCCTGATCTTTCAGAGCCTACTGACATCAGAAACTGGTTCTCTAGCTATGTGTATGAGCCTCTGTCTTTGAATACAGCTGATGCTTTTGGAGATTTTGTTTGTAGAGATAGAATAAATGAGAAGGATGAAGTTTTGGTTGAACCCAGCAACAGAGAAGAGGGAGAGGGTTTGGAGGGATTTAGTAAAAAGGAAAGTGATAGTGAAGAGGTTGATGTTGAGAAGATACAGTCAGAAGAGTTCGGAAAATATAACACCTCCTGCAGACATAATGATTTGTGTCTCGAATATATGCCTAAAATACTGCAAAACCATGGTATTAGTCAAGCAAAACATGGTGATACATCAAGCTTCAGCAAAGGAGATTCTCAATGCAAGCTAGATCAGAGAAACTTGTGCAGTAATGATGGCAAATGTCCACACAGTAGGGATTCCACTTTACAGGAGAGCTCAGAAGCAACGGTCTATGCAGAAGATTGCATACAACTCAAAAGTGATTCAGAGTTAATTTCAGTTAACGGAGGAGTGGTAAGCAAATCAACTGATGGAAGCAAAGAAAGAAAAGGCAAGGGAAAAGGATCATCAGAAGATGGTTTCATTACAACAAGGAAACGCAAATTTTCAGCAACAAATGATGTAAACTCTTCAAAAAGACCACCAGAGAGTGGTAAAGGAACAGTTCCATTAGCAAGCGGAAAGGATGTTATAAAAAGAAAGGCTTTAGCAGACACAACAAATATTCATCATTCTCCGGCAAAGATGATCACTGGGAAATGGAAGTGCCCTCAGCTTGGACTTGAGCAATGGATTCACAAGTTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

274

Amino Acids

30.39

Weight (kDa)

5.74

Isoelectric Point (pI)

49.1

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 710
AccB7I CCANNNNNTGG 1 cut(s) 668
AciI CCGC 1 cut(s) 696
AclWI GGATC 1 cut(s) 589
AcsI RAATTY 1 cut(s) 620
AcuI CTGAAG 1 cut(s) 340
AfiI CCNNNNNNNGG 2 cut(s) 668, 794
AgsI TTSAA 3 cut(s) 76, 146, 651
AluBI AGCT 6 cut(s) 51, 83, 354, 382, 453, 793
AluI AGCT 6 cut(s) 51, 83, 354, 382, 453, 793
Alw21I GWGCWC 1 cut(s) 455
Alw26I GTCTC 1 cut(s) 290
AlwI GGATC 1 cut(s) 589
AlwNI CAGNNNCTG 1 cut(s) 39
ApoI RAATTY 1 cut(s) 620
AsuHPI GGTGA 1 cut(s) 353
BaeGI GKGCMC 1 cut(s) 788
BanII GRGCYC 1 cut(s) 455
BarI GAAGNNNNNNTAC 2 cut(s) 468, 500
Bbv12I GWGCWC 1 cut(s) 455
BbvCI CCTCAGC 1 cut(s) 789
BccI CCATC 3 cut(s) 404, 542, 587
BclI TGATCA 1 cut(s) 765
BcoDI GTCTC 1 cut(s) 290
BfaI CTAG 2 cut(s) 48, 383
BfmI CTRYAG 1 cut(s) 265
BmrI ACTGGG 1 cut(s) 780
BmsI GCATC 1 cut(s) 76
BmuI ACTGGG 1 cut(s) 780
Bpu10I CCTNAGC 1 cut(s) 789
BpuEI CTTGAG 1 cut(s) 821
BsaJI CCNNGG 1 cut(s) 316
BsaXI ACNNNNNCTCC 4 cut(s) 87, 117, 516, 546
Bsc4I CCNNNNNNNGG 2 cut(s) 668, 794
Bse1I ACTGG 2 cut(s) 44, 775
Bse3DI GCAATG 1 cut(s) 812
BseDI CCNNGG 1 cut(s) 316
BseGI GGATG 2 cut(s) 136, 709
BseLI CCNNNNNNNGG 2 cut(s) 668, 794
BseMI GCAATG 1 cut(s) 812
BseMII CTCAG 2 cut(s) 468, 803
BseNI ACTGG 2 cut(s) 44, 775
BseRI GAGGAG 1 cut(s) 540
BseSI GKGCMC 1 cut(s) 788
BseYI CCCAGC 1 cut(s) 149
BsgI GTGCAG 1 cut(s) 421
BsiHKAI GWGCWC 1 cut(s) 455
BsiSI CCGG 1 cut(s) 755
BslI CCNNNNNNNGG 2 cut(s) 668, 794
BsmAI GTCTC 1 cut(s) 290
Bsp1286I GDGCHC 2 cut(s) 455, 788
Bsp143I GATC 4 cut(s) 10, 385, 581, 765
Bsp19I CCATGG 1 cut(s) 316
BspACI CCGC 1 cut(s) 696
BspCNI CTCAG 2 cut(s) 467, 802
BspMAI CTGCAG 1 cut(s) 269
BspPI GGATC 1 cut(s) 589
BsrDI GCAATG 1 cut(s) 812
BsrI ACTGG 2 cut(s) 44, 775
BssECI CCNNGG 1 cut(s) 316
BssMI GATC 4 cut(s) 10, 385, 581, 765
BssT1I CCWWGG 1 cut(s) 316
Bst4CI ACNGT 4 cut(s) 234, 428, 466, 682
Bst6I CTCTTC 4 cut(s) 156, 204, 234, 652
BstC8I GCNNGC 2 cut(s) 380, 694
BstDEI CTNAG 2 cut(s) 454, 789
BstDSI CCRYGG 1 cut(s) 316
BstF5I GGATG 2 cut(s) 136, 709
BstKTI GATC 4 cut(s) 13, 388, 584, 768
BstMAI GTCTC 1 cut(s) 290
BstMBI GATC 4 cut(s) 10, 385, 581, 765
BstSFI CTRYAG 1 cut(s) 265
BstSLI GKGCMC 1 cut(s) 788
BtgI CCRYGG 1 cut(s) 316
BtsCI GGATG 2 cut(s) 136, 709
BtsIMutI CAGTG 1 cut(s) 768
Cac8I GCNNGC 2 cut(s) 380, 694
CaiI CAGNNNCTG 1 cut(s) 39
CviAII CATG 2 cut(s) 317, 338
CviJI RGCY 9 cut(s) 22, 51, 64, 83, 354, 382, 453, 722, 793
CviKI_1 RGCY 9 cut(s) 22, 51, 64, 83, 354, 382, 453, 722, 793
DdeI CTNAG 2 cut(s) 454, 789
DpnI GATC 4 cut(s) 12, 387, 583, 767
DpnII GATC 4 cut(s) 10, 385, 581, 765
Eam1104I CTCTTC 4 cut(s) 156, 204, 234, 652
EarI CTCTTC 4 cut(s) 156, 204, 234, 652
Ecl136II GAGCTC 1 cut(s) 453
Eco130I CCWWGG 1 cut(s) 316
Eco24I GRGCYC 1 cut(s) 455
Eco53kI GAGCTC 1 cut(s) 453
Eco57I CTGAAG 1 cut(s) 340
EcoICRI GAGCTC 1 cut(s) 453
EcoT14I CCWWGG 1 cut(s) 316
EcoT38I GRGCYC 1 cut(s) 455
ErhI CCWWGG 1 cut(s) 316
FaeI CATG 2 cut(s) 320, 341
FalI AAGNNNNNCTT 2 cut(s) 706, 738
FatI CATG 2 cut(s) 316, 337
FbaI TGATCA 1 cut(s) 765
FokI GGATG 2 cut(s) 143, 716
FriOI GRGCYC 1 cut(s) 455
FspBI CTAG 2 cut(s) 48, 383
GsaI CCCAGC 1 cut(s) 153
HapII CCGG 1 cut(s) 755
Hin1II CATG 2 cut(s) 320, 341
HincII GTYRAC 1 cut(s) 520
HindII GTYRAC 1 cut(s) 520
HindIII AAGCTT 1 cut(s) 352
HinfI GANTC 4 cut(s) 368, 434, 500, 811
HpaI GTTAAC 1 cut(s) 520
HpaII CCGG 1 cut(s) 755
HphI GGTGA 1 cut(s) 353
Hpy166II GTNNAC 3 cut(s) 422, 520, 643
Hpy188I TCNGA 8 cut(s) 19, 35, 238, 248, 390, 457, 505, 589
Hpy188III TCNNGA 2 cut(s) 8, 287
Hpy8I GTNNAC 3 cut(s) 422, 520, 643
HpyAV CCTTC 1 cut(s) 121
HpyCH4III ACNGT 4 cut(s) 234, 428, 466, 682
HpyCH4V TGCA 6 cut(s) 267, 310, 378, 402, 473, 483
HpyF3I CTNAG 2 cut(s) 454, 789
Hsp92II CATG 2 cut(s) 320, 341
Ksp22I TGATCA 1 cut(s) 765
KspAI GTTAAC 1 cut(s) 520
Kzo9I GATC 4 cut(s) 10, 385, 581, 765
LpnPI CCDG 8 cut(s) 21, 25, 163, 277, 431, 675, 756, 768
LweI GCATC 1 cut(s) 76
MaeI CTAG 2 cut(s) 48, 383
MalI GATC 4 cut(s) 12, 387, 583, 767
MboI GATC 4 cut(s) 10, 385, 581, 765
MboII GAAGA 7 cut(s) 173, 221, 238, 251, 488, 602, 639
MhlI GDGCHC 2 cut(s) 455, 788
MluCI AATT 2 cut(s) 510, 620
MnlI CCTC 8 cut(s) 75, 157, 163, 172, 205, 271, 518, 798
MseI TTAA 2 cut(s) 509, 519
MspA1I CMGCKG 1 cut(s) 83
MspI CCGG 1 cut(s) 755
NcoI CCATGG 1 cut(s) 316
NdeII GATC 4 cut(s) 10, 385, 581, 765
NlaIII CATG 2 cut(s) 320, 341
PfeI GAWTC 4 cut(s) 368, 434, 500, 811
PflMI CCANNNNNTGG 1 cut(s) 668
PsiI TTATAA 1 cut(s) 710
Psp124BI GAGCTC 1 cut(s) 455
PspFI CCCAGC 1 cut(s) 149
PstI CTGCAG 1 cut(s) 269
PstNI CAGNNNCTG 1 cut(s) 39
PvuII CAGCTG 1 cut(s) 83
SacI GAGCTC 1 cut(s) 455
SaqAI TTAA 2 cut(s) 509, 519
Sau3AI GATC 4 cut(s) 10, 385, 581, 765
SduI GDGCHC 2 cut(s) 455, 788
SetI ASST 8 cut(s) 53, 85, 216, 263, 356, 384, 455, 795
SfaNI GCATC 1 cut(s) 76
SfcI CTRYAG 1 cut(s) 265
SmlI CTYRAG 1 cut(s) 800
SmoI CTYRAG 1 cut(s) 800
Sse9I AATT 2 cut(s) 510, 620
SsiI CCGC 1 cut(s) 696
SspI AATATT 1 cut(s) 742
SspMI CTAG 2 cut(s) 48, 383
SstI GAGCTC 1 cut(s) 455
StyI CCWWGG 1 cut(s) 316
TaaI ACNGT 4 cut(s) 234, 428, 466, 682
TaqI TCGA 1 cut(s) 288
TasI AATT 2 cut(s) 510, 620
TfiI GAWTC 4 cut(s) 368, 434, 500, 811
Tru1I TTAA 2 cut(s) 509, 519
Tru9I TTAA 2 cut(s) 509, 519
TscAI CASTG 1 cut(s) 775
TspDTI ATGAA 3 cut(s) 147, 589, 734
TspGWI ACGGA 1 cut(s) 537
TspRI CASTG 1 cut(s) 775
Van91I CCANNNNNTGG 1 cut(s) 668
XapI RAATTY 1 cut(s) 620
XspI CTAG 2 cut(s) 48, 383
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.