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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AcsI | RAATTY | 1 cut(s) | 250 |
| AgsI | TTSAA | 1 cut(s) | 215 |
| AluBI | AGCT | 2 cut(s) | 23, 120 |
| AluI | AGCT | 2 cut(s) | 23, 120 |
| ApeKI | GCWGC | 1 cut(s) | 20 |
| ApoI | RAATTY | 1 cut(s) | 250 |
| AsuHPI | GGTGA | 1 cut(s) | 302 |
| BbvI | GCAGC | 1 cut(s) | 32 |
| BccI | CCATC | 1 cut(s) | 149 |
| BciVI | GTATCC | 1 cut(s) | 139 |
| BfaI | CTAG | 1 cut(s) | 201 |
| BfuI | GTATCC | 1 cut(s) | 139 |
| BisI | GCNGC | 1 cut(s) | 21 |
| BlsI | GCNGC | 1 cut(s) | 22 |
| BpuEI | CTTGAG | 2 cut(s) | 27, 100 |
| Bse1I | ACTGG | 1 cut(s) | 113 |
| BseNI | ACTGG | 1 cut(s) | 113 |
| BseXI | GCAGC | 1 cut(s) | 32 |
| BslFI | GGGAC | 1 cut(s) | 64 |
| BsmFI | GGGAC | 1 cut(s) | 64 |
| BspQI | GCTCTTC | 1 cut(s) | 30 |
| BsrI | ACTGG | 1 cut(s) | 113 |
| Bst4CI | ACNGT | 2 cut(s) | 303, 363 |
| Bst6I | CTCTTC | 2 cut(s) | 30, 231 |
| BstC8I | GCNNGC | 1 cut(s) | 315 |
| BstDEI | CTNAG | 1 cut(s) | 344 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 354 |
| BstV1I | GCAGC | 1 cut(s) | 32 |
| BsuI | GTATCC | 1 cut(s) | 139 |
| BtsI | GCAGTG | 1 cut(s) | 274 |
| BtsIMutI | CAGTG | 1 cut(s) | 274 |
| Cac8I | GCNNGC | 1 cut(s) | 315 |
| CseI | GACGC | 1 cut(s) | 335 |
| CviJI | RGCY | 5 cut(s) | 23, 91, 120, 200, 313 |
| CviKI_1 | RGCY | 5 cut(s) | 23, 91, 120, 200, 313 |
| DdeI | CTNAG | 1 cut(s) | 344 |
| Eam1104I | CTCTTC | 2 cut(s) | 30, 231 |
| EarI | CTCTTC | 2 cut(s) | 30, 231 |
| FaiI | YATR | 4 cut(s) | 99, 123, 129, 260 |
| FaqI | GGGAC | 1 cut(s) | 64 |
| Fnu4HI | GCNGC | 1 cut(s) | 21 |
| Fsp4HI | GCNGC | 1 cut(s) | 21 |
| FspBI | CTAG | 1 cut(s) | 201 |
| GluI | GCNGC | 1 cut(s) | 21 |
| HgaI | GACGC | 1 cut(s) | 335 |
| HinfI | GANTC | 3 cut(s) | 68, 131, 167 |
| HphI | GGTGA | 1 cut(s) | 302 |
| Hpy188I | TCNGA | 1 cut(s) | 88 |
| Hpy188III | TCNNGA | 1 cut(s) | 306 |
| HpyAV | CCTTC | 2 cut(s) | 147, 190 |
| HpyCH4III | ACNGT | 2 cut(s) | 303, 363 |
| HpyCH4V | TGCA | 2 cut(s) | 177, 317 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 354 |
| HpyF3I | CTNAG | 1 cut(s) | 344 |
| LguI | GCTCTTC | 1 cut(s) | 30 |
| LpnPI | CCDG | 3 cut(s) | 42, 94, 291 |
| Lsp1109I | GCAGC | 1 cut(s) | 32 |
| MaeI | CTAG | 1 cut(s) | 201 |
| MaeIII | GTNAC | 2 cut(s) | 69, 323 |
| MboII | GAAGA | 3 cut(s) | 17, 248, 307 |
| MluCI | AATT | 2 cut(s) | 250, 319 |
| MlyI | GAGTC | 1 cut(s) | 77 |
| MnlI | CCTC | 4 cut(s) | 232, 240, 274, 302 |
| MseI | TTAA | 1 cut(s) | 48 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 354 |
| NmuCI | GTSAC | 2 cut(s) | 69, 323 |
| PciSI | GCTCTTC | 1 cut(s) | 30 |
| PfeI | GAWTC | 2 cut(s) | 131, 167 |
| PkrI | GCNGC | 1 cut(s) | 22 |
| PleI | GAGTC | 1 cut(s) | 76 |
| PpsI | GAGTC | 1 cut(s) | 76 |
| SapI | GCTCTTC | 1 cut(s) | 30 |
| SaqAI | TTAA | 1 cut(s) | 48 |
| SatI | GCNGC | 1 cut(s) | 21 |
| SchI | GAGTC | 1 cut(s) | 77 |
| SetI | ASST | 5 cut(s) | 8, 25, 61, 122, 285 |
| SmlI | CTYRAG | 2 cut(s) | 42, 115 |
| SmoI | CTYRAG | 2 cut(s) | 42, 115 |
| Sse9I | AATT | 2 cut(s) | 250, 319 |
| SspMI | CTAG | 1 cut(s) | 201 |
| TaaI | ACNGT | 2 cut(s) | 303, 363 |
| TasI | AATT | 2 cut(s) | 250, 319 |
| TfiI | GAWTC | 2 cut(s) | 131, 167 |
| Tru1I | TTAA | 1 cut(s) | 48 |
| Tru9I | TTAA | 1 cut(s) | 48 |
| TscAI | CASTG | 1 cut(s) | 274 |
| TseFI | GTSAC | 2 cut(s) | 69, 323 |
| TseI | GCWGC | 1 cut(s) | 20 |
| Tsp45I | GTSAC | 2 cut(s) | 69, 323 |
| TspDTI | ATGAA | 3 cut(s) | 129, 144, 249 |
| TspGWI | ACGGA | 1 cut(s) | 66 |
| TspRI | CASTG | 1 cut(s) | 274 |
| XapI | RAATTY | 1 cut(s) | 250 |
| XspI | CTAG | 1 cut(s) | 201 |