FvH4_3g24140

Cytochrome p450

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0000398
Physical Location & Seq
Reverse (-)
17152487 .. 17155244
2758 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g24140.t1

Sequence Viewer

Length: 1110 bp
ATGAGTTGCAAGATAGTGTTTGGGAAGAAATATGCAGACAAGGAATTCGACAACAAAGGTTTCAAGTCTGTGATCCAACAAGGCATGCAATTTGCAGCTGCACCGAACTTGGGAGATTACATCCGTTGTATTGCTCCACTTGATCTGCAAGGGTTCACTAAGCGCATGAAGGCTGTTAGCAAGGTGTTCAATGAGTTTTTCGAGAAGATCATTGATGAACATCTTCAATCGAAGGATGAGGGAAAGACGAAGGACTTTGTATTGCTCCACTTGATCTGCAAGGAATCTAAATACCTAATTGAACGTTCCAACATCAAAGCAATCATGTTGGACATGCTGGCGGGCTCGATGGACACATCAGTGACAACAGTTAAGTGGGCCCTCTTTGAACTCATAAAGCATCCACAACTAATGAAGAAGAATGGTGGAGGATCAGACCTGGAGAAGCTGGAGTACCTGGACATGGTAGTGAAAGAAACCTTGAGGCTACATCCAGTGGCACCATTGTTGCTTCCCTATGCATCCACTGAGGATTGCTCTGTTAATGGCTTCCACATACCCAAAAAATCGCGTATTATCATAAACGTGTATGCAATTGGAAGAGACCCTAATGCTTGGACCGATGCAGAGGAGTTCATACCCGAAAGGTTTGTAGGGAGTAATATAGACATGAGGGGGAACCATTTTCAGCTTATTCCATTCGGGTCTGGTCGTAGAGGTTGCCCTGGAATTCGATCAGTTGGGGCTAGTTGTGAGATTCTGAATCTGCCGCTCACCTTGTTGACAGATGAAGCCATTGAGAAAGTGGAGTGGACGTCGGGTCATGTCGATGTTTGTGGTTTGTTAGAGCACTATGAGTCCGGCTGCGGTGGAGGACCAGTTCTGAAACTTAAGCCCATCGAGATTGAGTCGATGAAAACACCTTTTTTGACTTTGGAGGGGCGGCTAGGCGGATCATCTTCTGGTAATCCGTTTGCAGAGAGTGAGGTCGTCAACCTGTTTGCTGCATCAGGCTCAACAGATTTGGCTGAAAGTGGGATCCCTAATCTGTCGTTCTATATCCTTGGTATTTCCGCCTTTGAACTTGTAGCGCAATTGGCACATTTCTGA

Protein Analysis

370

Amino Acids

40.74

Weight (kDa)

5.91

Isoelectric Point (pI)

38.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 2 - 244 3.5e-56 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000398)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g21960 FvH4_3g24140 FvH4_3g24150 FvH4_7g09230 FvH4_7g09260
malus_domestica MD00G1169700.v1.1 MD00G1169800.v1.1 MD01G1016400.v1.1 MD03G1188000.v1.1 MD03G1188100.v1.1 MD11G1219700.v1.1 MD11G1219900.v1.1 MD11G1220200.v1.1 MD11G1220400.v1.1 MD11G1220600.v1.1
prunus_persica Prupe.2G006500_v2.0.a1 Prupe.4G235500_v2.0.a1 Prupe.4G235600_v2.0.a1 Prupe.4G235700_v2.0.a1 Prupe.4G235700_v2.0.a1 Prupe.4G235800_v2.0.a1 Prupe.4G235900_v2.0.a1 Prupe.6G176500_v2.0.a1
pyrus_communis pycom01g04750 pycom03g14060 pycom11g19360 pycom11g19370 pycom11g19380 pycom11g19390 pycom11g19400 pycom11g19460
rosa_chinensis RchiOBHm_Chr1g0345321 RchiOBHm_Chr1g0345511 RchiOBHm_Chr1g0345781 RchiOBHm_Chr2g0115981 RchiOBHm_Chr5g0043151 RchiOBHm_Chr5g0043161 RchiOBHm_Chr5g0043901 RchiOBHm_Chr6g0274841
rosa_laevigata RLG00000013516 RLG00000018229 RLG00000028840 RLG00000028923 RLG00000034169 RLG00000034223
rosa_multiflora Rmu_co8474237.1_g000001 Rmu_sc0000698.1_g000004 Rmu_sc0000698.1_g000021 Rmu_sc0000698.1_g000023 Rmu_sc0000698.1_g000056 Rmu_sc0000698.1_g000057 Rmu_sc0000698.1_g000122 Rmu_sc0000698.1_g000123 Rmu_sc0000698.1_g000124 Rmu_sc0001292.1_g000005 Rmu_sc0001292.1_g000006 Rmu_sc0001349.1_g000002 Rmu_sc0001692.1_g000035 Rmu_sc0002079.1_g000035 Rmu_sc0002079.1_g000045 Rmu_sc0002329.1_g000017 Rmu_sc0008148.1_g000056 Rmu_sc0008148.1_g000057 Rmu_sc0008148.1_g000058 Rmu_sc0008148.1_g000062 Rmu_sc0008611.1_g000010 Rmu_sc0009227.1_g000007
rosa_roxburghii Rroxscaffold_1G00011260 Rroxscaffold_1G00037590 Rroxscaffold_2G00127610 Rroxscaffold_4G00309120 Rroxscaffold_7G00193880
rosa_rugosa Rorug01G0177400 Rorug02G0202400 Rorug05G0204600 Rorug05G0209000 Rorug06G0085100 Rorug06G0085200
rosa_samantha Rh1BG154400 Rh1DG186600 Rh2CG265200 Rh2DG267000 Rh5AG289700 Rh5AG289800 Rh5AG290000 Rh5AG293200 Rh5CG326500 Rh5DG305700 Rh5DG305800 Rh5DG310100 Rh6BG204200 Rh6CG204500 Rh6DG195700
rosa_wichuraiana Rw1G016100 Rw1G016470 Rw2G020290 Rw5G026830 Rw5G026910 Rw5G026920 Rw5G026930 Rw6G017460

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 818
AccB1I GGYRCC 1 cut(s) 499
AccBSI CCGCTC 1 cut(s) 772
AccII CGCG 1 cut(s) 571
AciI CCGC 6 cut(s) 341, 770, 867, 943, 951, 1074
AclI AACGTT 1 cut(s) 304
AclWI GGATC 5 cut(s) 67, 439, 961, 1033, 1046
AcsI RAATTY 2 cut(s) 44, 729
AcyI GRCGYC 1 cut(s) 815
AfaI GTAC 1 cut(s) 455
AfiI CCNNNNNNNGG 2 cut(s) 110, 463
AflII CTTAAG 1 cut(s) 890
AflIII ACRYGT 1 cut(s) 585
AgsI TTSAA 6 cut(s) 64, 190, 227, 302, 389, 1082
AhdI GACNNNNNGTC 1 cut(s) 819
AjnI CCWGG 3 cut(s) 438, 456, 724
AleI CACNNNNGTG 1 cut(s) 359
AloI GAACNNNNNNTCC 2 cut(s) 864, 896
AluBI AGCT 3 cut(s) 98, 448, 691
AluI AGCT 3 cut(s) 98, 448, 691
Alw21I GWGCWC 1 cut(s) 852
Alw26I GTCTC 1 cut(s) 597
AlwI GGATC 5 cut(s) 67, 439, 961, 1033, 1046
AoxI GGCC 1 cut(s) 378
ApaI GGGCCC 1 cut(s) 382
ApeKI GCWGC 4 cut(s) 95, 98, 864, 1004
ApoI RAATTY 2 cut(s) 44, 729
ArsI GACNNNNNNTTYG 2 cut(s) 29, 61
Asp700I GAANNNNTTC 1 cut(s) 222
AspLEI GCGC 2 cut(s) 165, 1093
AspS9I GGNCC 4 cut(s) 378, 379, 618, 875
AsuHPI GGTGA 1 cut(s) 766
AvaII GGWCC 2 cut(s) 618, 875
BaeGI GKGCMC 1 cut(s) 382
BamHI GGATCC 1 cut(s) 1038
BanI GGYRCC 1 cut(s) 499
BanII GRGCYC 2 cut(s) 347, 382
BarI GAAGNNNNNNTAC 2 cut(s) 437, 469
Bbv12I GWGCWC 1 cut(s) 852
BbvI GCAGC 4 cut(s) 85, 107, 851, 991
BccI CCATC 2 cut(s) 343, 905
BciT130I CCWGG 3 cut(s) 440, 458, 726
BcoDI GTCTC 1 cut(s) 597
BfaI CTAG 2 cut(s) 747, 947
BfrI CTTAAG 1 cut(s) 890
BisI GCNGC 6 cut(s) 96, 99, 770, 865, 944, 1005
BlsI GCNGC 6 cut(s) 97, 100, 771, 866, 945, 1006
Bme1390I CCNGG 3 cut(s) 440, 458, 726
Bme18I GGWCC 2 cut(s) 618, 875
BmeRI GACNNNNNGTC 1 cut(s) 819
BmgT120I GGNCC 4 cut(s) 378, 379, 618, 875
BmiI GGNNCC 4 cut(s) 380, 501, 680, 1040
BmrFI CCNGG 3 cut(s) 440, 458, 726
BmsI GCATC 4 cut(s) 409, 530, 613, 1016
BplI GAGNNNNNCTC 2 cut(s) 521, 553
BpmI CTGGAG 2 cut(s) 461, 470
BpuEI CTTGAG 1 cut(s) 502
BsaBI GATNNNNATC 1 cut(s) 219
BsaHI GRCGYC 1 cut(s) 815
BsaI GGTCTC 1 cut(s) 597
BsaJI CCNNGG 2 cut(s) 724, 1063
BsaXI ACNNNNNCTCC 4 cut(s) 800, 830, 864, 894
Bsc4I CCNNNNNNNGG 2 cut(s) 110, 463
Bse1I ACTGG 2 cut(s) 494, 878
Bse8I GATNNNNATC 1 cut(s) 219
BseBI CCWGG 3 cut(s) 440, 458, 726
BseDI CCNNGG 2 cut(s) 724, 1063
BseGI GGATG 5 cut(s) 120, 241, 400, 490, 521
BseJI GATNNNNATC 1 cut(s) 219
BseLI CCNNNNNNNGG 2 cut(s) 110, 463
BseMII CTCAG 1 cut(s) 519
BseNI ACTGG 2 cut(s) 494, 878
BseRI GAGGAG 1 cut(s) 644
BseSI GKGCMC 1 cut(s) 382
BseXI GCAGC 4 cut(s) 85, 107, 851, 991
BsgI GTGCAG 1 cut(s) 84
Bsh1236I CGCG 1 cut(s) 571
BshFI GGCC 1 cut(s) 380
BshNI GGYRCC 1 cut(s) 499
BsiHKAI GWGCWC 1 cut(s) 852
BsiSI CCGG 1 cut(s) 861
BslI CCNNNNNNNGG 2 cut(s) 110, 463
BsmAI GTCTC 1 cut(s) 597
BsnI GGCC 1 cut(s) 380
Bso31I GGTCTC 1 cut(s) 597
Bsp120I GGGCCC 1 cut(s) 378
Bsp1286I GDGCHC 3 cut(s) 347, 382, 852
Bsp143I GATC 8 cut(s) 72, 142, 207, 273, 431, 734, 953, 1038
BspACI CCGC 6 cut(s) 341, 770, 867, 943, 951, 1074
BspANI GGCC 1 cut(s) 380
BspCNI CTCAG 1 cut(s) 520
BspFNI CGCG 1 cut(s) 571
BspLI GGNNCC 4 cut(s) 380, 501, 680, 1040
BspPI GGATC 5 cut(s) 67, 439, 961, 1033, 1046
BspT107I GGYRCC 1 cut(s) 499
BspTI CTTAAG 1 cut(s) 890
BspTNI GGTCTC 1 cut(s) 597
BsrBI CCGCTC 1 cut(s) 772
BsrI ACTGG 2 cut(s) 494, 878
BssECI CCNNGG 2 cut(s) 724, 1063
BssMI GATC 8 cut(s) 72, 142, 207, 273, 431, 734, 953, 1038
BssNI GRCGYC 1 cut(s) 815
BssT1I CCWWGG 1 cut(s) 1063
Bst2UI CCWGG 3 cut(s) 440, 458, 726
Bst4CI ACNGT 1 cut(s) 370
Bst6I CTCTTC 1 cut(s) 595
BstACI GRCGYC 1 cut(s) 815
BstAFI CTTAAG 1 cut(s) 890
BstC8I GCNNGC 3 cut(s) 86, 339, 343
BstDEI CTNAG 2 cut(s) 159, 528
BstF5I GGATG 5 cut(s) 120, 241, 400, 490, 521
BstFNI CGCG 1 cut(s) 571
BstHHI GCGC 2 cut(s) 165, 1093
BstKTI GATC 8 cut(s) 75, 145, 210, 276, 434, 737, 956, 1041
BstMAI GTCTC 1 cut(s) 597
BstMBI GATC 8 cut(s) 72, 142, 207, 273, 431, 734, 953, 1038
BstMWI GCNNNNNNNGC 1 cut(s) 1097
BstNI CCWGG 3 cut(s) 440, 458, 726
BstNSI RCATGY 2 cut(s) 88, 337
BstSCI CCNGG 3 cut(s) 438, 456, 724
BstSLI GKGCMC 1 cut(s) 382
BstUI CGCG 1 cut(s) 571
BstV1I GCAGC 4 cut(s) 85, 107, 851, 991
BstX2I RGATCY 1 cut(s) 1038
BstYI RGATCY 1 cut(s) 1038
BsuRI GGCC 1 cut(s) 380
BtsCI GGATG 5 cut(s) 120, 241, 400, 490, 521
BtsIMutI CAGTG 3 cut(s) 366, 501, 525
Cac8I GCNNGC 3 cut(s) 86, 339, 343
CfoI GCGC 2 cut(s) 165, 1093
Cfr13I GGNCC 4 cut(s) 378, 379, 618, 875
Csp6I GTAC 1 cut(s) 454
CviAII CATG 7 cut(s) 85, 166, 325, 334, 463, 670, 824
CviQI GTAC 1 cut(s) 454
DdeI CTNAG 2 cut(s) 159, 528
DpnI GATC 8 cut(s) 74, 144, 209, 275, 433, 736, 955, 1040
DpnII GATC 8 cut(s) 72, 142, 207, 273, 431, 734, 953, 1038
DriI GACNNNNNGTC 1 cut(s) 819
Eam1104I CTCTTC 1 cut(s) 595
Eam1105I GACNNNNNGTC 1 cut(s) 819
EarI CTCTTC 1 cut(s) 595
EciI GGCGGA 2 cut(s) 966, 1063
Eco130I CCWWGG 1 cut(s) 1063
Eco24I GRGCYC 2 cut(s) 347, 382
Eco31I GGTCTC 1 cut(s) 597
Eco47I GGWCC 2 cut(s) 618, 875
EcoO109I RGGNCCY 1 cut(s) 379
EcoRI GAATTC 2 cut(s) 44, 729
EcoRII CCWGG 3 cut(s) 438, 456, 724
EcoT14I CCWWGG 1 cut(s) 1063
EcoT22I ATGCAT 1 cut(s) 523
EcoT38I GRGCYC 2 cut(s) 347, 382
ErhI CCWWGG 1 cut(s) 1063
FaeI CATG 7 cut(s) 88, 169, 328, 337, 466, 673, 827
FatI CATG 7 cut(s) 84, 165, 324, 333, 462, 669, 823
FauI CCCGC 1 cut(s) 334
Fnu4HI GCNGC 6 cut(s) 96, 99, 770, 865, 944, 1005
FokI GGATG 5 cut(s) 107, 248, 387, 477, 508
FriOI GRGCYC 2 cut(s) 347, 382
Fsp4HI GCNGC 6 cut(s) 96, 99, 770, 865, 944, 1005
FspBI CTAG 2 cut(s) 747, 947
GlaI GCGC 2 cut(s) 164, 1092
GluI GCNGC 6 cut(s) 96, 99, 770, 865, 944, 1005
GsuI CTGGAG 2 cut(s) 461, 470
HaeIII GGCC 1 cut(s) 380
HapII CCGG 1 cut(s) 861
HhaI GCGC 2 cut(s) 165, 1093
Hin1I GRCGYC 1 cut(s) 815
Hin1II CATG 7 cut(s) 88, 169, 328, 337, 466, 673, 827
Hin6I GCGC 2 cut(s) 163, 1091
HinP1I GCGC 2 cut(s) 163, 1091
HincII GTYRAC 2 cut(s) 783, 994
HindII GTYRAC 2 cut(s) 783, 994
HinfI GANTC 5 cut(s) 284, 757, 763, 857, 908
HpaII CCGG 1 cut(s) 861
HphI GGTGA 1 cut(s) 766
Hpy166II GTNNAC 4 cut(s) 156, 783, 813, 994
Hpy188I TCNGA 4 cut(s) 436, 762, 885, 1109
Hpy188III TCNNGA 2 cut(s) 202, 901
Hpy8I GTNNAC 4 cut(s) 156, 783, 813, 994
Hpy99I CGWCG 1 cut(s) 820
HpyAV CCTTC 3 cut(s) 163, 226, 244
HpyCH4III ACNGT 1 cut(s) 370
HpyCH4IV ACGT 3 cut(s) 304, 585, 815
HpyF10VI GCNNNNNNNGC 1 cut(s) 1097
HpyF3I CTNAG 2 cut(s) 159, 528
HpySE526I ACGT 3 cut(s) 304, 585, 815
Hsp92I GRCGYC 1 cut(s) 815
Hsp92II CATG 7 cut(s) 88, 169, 328, 337, 466, 673, 827
HspAI GCGC 2 cut(s) 163, 1091
Kzo9I GATC 8 cut(s) 72, 142, 207, 273, 431, 734, 953, 1038
LmnI GCTCC 2 cut(s) 139, 270
Lsp1109I GCAGC 4 cut(s) 85, 107, 851, 991
LweI GCATC 4 cut(s) 409, 530, 613, 1016
MaeI CTAG 2 cut(s) 747, 947
MaeII ACGT 3 cut(s) 304, 585, 815
MaeIII GTNAC 1 cut(s) 361
MalI GATC 8 cut(s) 74, 144, 209, 275, 433, 736, 955, 1040
MbiI CCGCTC 1 cut(s) 772
MboI GATC 8 cut(s) 72, 142, 207, 273, 431, 734, 953, 1038
MboII GAAGA 7 cut(s) 37, 215, 217, 427, 430, 612, 951
MfeI CAATTG 2 cut(s) 594, 1094
MflI RGATCY 1 cut(s) 1038
MhlI GDGCHC 3 cut(s) 347, 382, 852
MluCI AATT 6 cut(s) 44, 89, 297, 594, 729, 1094
MlyI GAGTC 2 cut(s) 866, 917
MmeI TCCRAC 3 cut(s) 100, 309, 333
Mph1103I ATGCAT 1 cut(s) 523
MroXI GAANNNNTTC 1 cut(s) 222
MseI TTAA 3 cut(s) 372, 543, 891
MslI CAYNNNNRTG 4 cut(s) 359, 467, 584, 828
MspA1I CMGCKG 1 cut(s) 98
MspCI CTTAAG 1 cut(s) 890
MspI CCGG 1 cut(s) 861
MspR9I CCNGG 3 cut(s) 440, 458, 726
MunI CAATTG 2 cut(s) 594, 1094
MvaI CCWGG 3 cut(s) 440, 458, 726
MvnI CGCG 1 cut(s) 571
MwoI GCNNNNNNNGC 1 cut(s) 1097
NdeII GATC 8 cut(s) 72, 142, 207, 273, 431, 734, 953, 1038
NlaIII CATG 7 cut(s) 88, 169, 328, 337, 466, 673, 827
NlaIV GGNNCC 4 cut(s) 380, 501, 680, 1040
NmuCI GTSAC 1 cut(s) 361
NsiI ATGCAT 1 cut(s) 523
NspI RCATGY 2 cut(s) 88, 337
OliI CACNNNNGTG 1 cut(s) 359
PaeI GCATGC 1 cut(s) 88
PdmI GAANNNNTTC 1 cut(s) 222
PfeI GAWTC 3 cut(s) 284, 757, 763
PkrI GCNGC 6 cut(s) 97, 100, 771, 866, 945, 1006
PleI GAGTC 2 cut(s) 865, 916
PpsI GAGTC 2 cut(s) 865, 916
Psp1406I AACGTT 1 cut(s) 304
Psp6I CCWGG 3 cut(s) 438, 456, 724
PspGI CCWGG 3 cut(s) 438, 456, 724
PspN4I GGNNCC 4 cut(s) 380, 501, 680, 1040
PspOMI GGGCCC 1 cut(s) 378
PspPI GGNCC 4 cut(s) 378, 379, 618, 875
PsuI RGATCY 1 cut(s) 1038
PvuII CAGCTG 1 cut(s) 98
RsaI GTAC 1 cut(s) 455
RsaNI GTAC 1 cut(s) 454
RseI CAYNNNNRTG 4 cut(s) 359, 467, 584, 828
SaqAI TTAA 3 cut(s) 372, 543, 891
SatI GCNGC 6 cut(s) 96, 99, 770, 865, 944, 1005
Sau3AI GATC 8 cut(s) 72, 142, 207, 273, 431, 734, 953, 1038
Sau96I GGNCC 4 cut(s) 378, 379, 618, 875
SchI GAGTC 2 cut(s) 866, 917
ScrFI CCNGG 3 cut(s) 440, 458, 726
SduI GDGCHC 3 cut(s) 347, 382, 852
SfaNI GCATC 4 cut(s) 409, 530, 613, 1016
SinI GGWCC 2 cut(s) 618, 875
SmiMI CAYNNNNRTG 4 cut(s) 359, 467, 584, 828
SmlI CTYRAG 2 cut(s) 481, 890
SmoI CTYRAG 2 cut(s) 481, 890
SphI GCATGC 1 cut(s) 88
Sse9I AATT 6 cut(s) 44, 89, 297, 594, 729, 1094
SsiI CCGC 6 cut(s) 341, 770, 867, 943, 951, 1074
SspMI CTAG 2 cut(s) 747, 947
StyD4I CCNGG 3 cut(s) 438, 456, 724
StyI CCWWGG 1 cut(s) 1063
TaaI ACNGT 1 cut(s) 370
TaiI ACGT 3 cut(s) 307, 588, 818
TaqI TCGA 8 cut(s) 48, 201, 230, 347, 733, 828, 900, 911
TaqII GACCGA 1 cut(s) 635
TasI AATT 6 cut(s) 44, 89, 297, 594, 729, 1094
TauI GCSGC 2 cut(s) 772, 946
TfiI GAWTC 3 cut(s) 284, 757, 763
Tru1I TTAA 3 cut(s) 372, 543, 891
Tru9I TTAA 3 cut(s) 372, 543, 891
TscAI CASTG 3 cut(s) 366, 501, 532
TseFI GTSAC 1 cut(s) 361
TseI GCWGC 4 cut(s) 95, 98, 864, 1004
Tsp45I GTSAC 1 cut(s) 361
TspDTI ATGAA 6 cut(s) 182, 231, 428, 625, 804, 929
TspGWI ACGGA 2 cut(s) 113, 960
TspRI CASTG 3 cut(s) 366, 501, 532
Vha464I CTTAAG 1 cut(s) 890
VpaK11BI GGWCC 2 cut(s) 618, 875
XapI RAATTY 2 cut(s) 44, 729
XceI RCATGY 2 cut(s) 88, 337
XcmI CCANNNNNNNNNTGG 1 cut(s) 802
XmnI GAANNNNTTC 1 cut(s) 222
XspI CTAG 2 cut(s) 747, 947
ZraI GACGTC 1 cut(s) 816
Zsp2I ATGCAT 1 cut(s) 523
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.