Rw2G020290

Cytochrome p450

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0000398
Physical Location & Seq
Forward (+)
27517963 .. 27519808
1846 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G020290.1

Sequence Viewer

Length: 1500 bp
ATGGGTTGGATTTGGGCAATTGGGTTGCTTGCTATTGTTTATGTACTGCAAGCATGGATTTCCAAAAGCAATAACAAGAAGATCAAAAAATTACCTCCCGGTCCAAGAGGTTTTCCTATTCTCGGAAACCTCCTCATGTTAGGGGAGTTCCCTCATGTCGATCTTCACCGACTAGCCCAAAAGCACGGTGACATCATGCACCTGCGCTTAGGCCTGGTGCCTACCATTGTTGTCTCATCCCCTCAAGCGGCTGAGTTGTTTCTTAAAACGCACGACCTAGCTTTTGCCACTAGGCCACCGCATGAAGCTGCAAAGCACATTTCTTATGGCCAAAGGAACTTATCCTATGCCGAGTATGGGTCTTATTGGCGCACCATTCGCAAGATGTGCACCCTTGAATTACTCAGCAACCATAAAATCAATTCTTTCAAGTCCATGAGGAAAGAAGAGCTTGATCTTTTCATTAACTTCGTCCGAGAGGCTTCCCACGATCATGCTACGGTTGATCTCAGCGCCAAGGTTTCGTCTCTCAATGCAGACATGACGTGCCGAATGGTGTTTGGAAAGAAGTACATGGATAAGGAATTTGATGAGAAGGGTTTCCAGGCTTGGATTCAAGAGGGTATGCATCTAGCAGGCACCCCCAACTTTGGAGATTACCTTCCTTTTCTTGCTCCATTTGACCTCCAGGGTTTAACTAAGCGAATGAAGGCTGTTAGCAAGGTGTTTGATGACTTTTTTGAGAAGATAATTGACGAGCACATCGAATCTCAGGATGATCCAGAAAGGACCAAAGACTTTGTCGATGTCATGTTGGGTTTCATGGGATCGCAAGAATCTGAGTACAAAATTGAACGCTCCAATATCAAAGCCATAATTCTGGACATGGTTGCCGGTGCAATGGACACGTCAGCAACGGCAATTGAGTGGACACTTTCCGAACTCATCAGGCACCCTCGGGTGATGAAGAAACTCCAGAAAGAGTTAGAAGATGTAGTAGGCATGGAGAGAATGGTGGATGAATCAGACTTGGAGAAGTTAGAGTACTTGAACATGGTGTTGAAGGAAAACATGAGGCTACATCCAGTTGCTCCATTGTTGATCCCACATGCAGCCATTGAAGACTGCATTGTTAACGGCTTCCACATACCCCAAAAATCGCACGTCATCGTAAATATTTGGGCAGTTGGGAGAGACCCTAGTGCTTGGACTGATCCTGACAAGTTCATACCGGAAAGGTTTGCCGGTAGTAATATAGATCTCCGTGGACGGGACTTCCAACTCCTTCCGTTTGGTTCTGGCCGAAGAGGTTGCCCTGGAATGCATCTAGGGTTAACTGTGGTCCTTCTGGTGGTGGCGCAACTTATGCATTGCTTTAATTGGAAACTTCCGGATAATATGTTGCCAAATGAGTTGGACATGACTGAGGAGTTTGGTCTAACAGTTCCAAGGGCCAAACATCTACTAGCCATTCCTACTTATCGTCTCCGACAACAATAA

Protein Analysis

499

Amino Acids

56.94

Weight (kDa)

6.58

Isoelectric Point (pI)

39.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 32 - 487 1.8e-104 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000398)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g21960 FvH4_3g24140 FvH4_3g24150 FvH4_7g09230 FvH4_7g09260
malus_domestica MD00G1169700.v1.1 MD00G1169800.v1.1 MD01G1016400.v1.1 MD03G1188000.v1.1 MD03G1188100.v1.1 MD11G1219700.v1.1 MD11G1219900.v1.1 MD11G1220200.v1.1 MD11G1220400.v1.1 MD11G1220600.v1.1
prunus_persica Prupe.2G006500_v2.0.a1 Prupe.4G235500_v2.0.a1 Prupe.4G235600_v2.0.a1 Prupe.4G235700_v2.0.a1 Prupe.4G235700_v2.0.a1 Prupe.4G235800_v2.0.a1 Prupe.4G235900_v2.0.a1 Prupe.6G176500_v2.0.a1
pyrus_communis pycom01g04750 pycom03g14060 pycom11g19360 pycom11g19370 pycom11g19380 pycom11g19390 pycom11g19400 pycom11g19460
rosa_chinensis RchiOBHm_Chr1g0345321 RchiOBHm_Chr1g0345511 RchiOBHm_Chr1g0345781 RchiOBHm_Chr2g0115981 RchiOBHm_Chr5g0043151 RchiOBHm_Chr5g0043161 RchiOBHm_Chr5g0043901 RchiOBHm_Chr6g0274841
rosa_laevigata RLG00000013516 RLG00000018229 RLG00000028840 RLG00000028923 RLG00000034169 RLG00000034223
rosa_multiflora Rmu_co8474237.1_g000001 Rmu_sc0000698.1_g000004 Rmu_sc0000698.1_g000021 Rmu_sc0000698.1_g000023 Rmu_sc0000698.1_g000056 Rmu_sc0000698.1_g000057 Rmu_sc0000698.1_g000122 Rmu_sc0000698.1_g000123 Rmu_sc0000698.1_g000124 Rmu_sc0001292.1_g000005 Rmu_sc0001292.1_g000006 Rmu_sc0001349.1_g000002 Rmu_sc0001692.1_g000035 Rmu_sc0002079.1_g000035 Rmu_sc0002079.1_g000045 Rmu_sc0002329.1_g000017 Rmu_sc0008148.1_g000056 Rmu_sc0008148.1_g000057 Rmu_sc0008148.1_g000058 Rmu_sc0008148.1_g000062 Rmu_sc0008611.1_g000010 Rmu_sc0009227.1_g000007
rosa_roxburghii Rroxscaffold_1G00011260 Rroxscaffold_1G00037590 Rroxscaffold_2G00127610 Rroxscaffold_4G00309120 Rroxscaffold_7G00193880
rosa_rugosa Rorug01G0177400 Rorug02G0202400 Rorug05G0204600 Rorug05G0209000 Rorug06G0085100 Rorug06G0085200
rosa_samantha Rh1BG154400 Rh1DG186600 Rh2CG265200 Rh2DG267000 Rh5AG289700 Rh5AG289800 Rh5AG290000 Rh5AG293200 Rh5CG326500 Rh5DG305700 Rh5DG305800 Rh5DG310100 Rh6BG204200 Rh6CG204500 Rh6DG195700
rosa_wichuraiana Rw1G016100 Rw1G016470 Rw2G020290 Rw5G026830 Rw5G026910 Rw5G026920 Rw5G026930 Rw6G017460

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 210
Acc36I ACCTGC 1 cut(s) 210
AccB1I GGYRCC 3 cut(s) 217, 638, 951
AccIII TCCGGA 1 cut(s) 1390
AciI CCGC 2 cut(s) 248, 299
AclWI GGATC 4 cut(s) 773, 835, 1096, 1208
AcoI YGGCCR 2 cut(s) 328, 1300
AcsI RAATTY 1 cut(s) 584
AfaI GTAC 4 cut(s) 45, 572, 845, 1046
AfiI CCNNNNNNNGG 6 cut(s) 122, 247, 357, 650, 1270, 1351
AflIII ACRYGT 1 cut(s) 906
AgsI TTSAA 7 cut(s) 398, 430, 617, 854, 1051, 1063, 1121
AjiI CACGTC 3 cut(s) 546, 909, 1165
AjnI CCWGG 4 cut(s) 213, 603, 687, 1315
AluBI AGCT 3 cut(s) 281, 308, 451
AluI AGCT 3 cut(s) 281, 308, 451
Alw21I GWGCWC 2 cut(s) 392, 762
Alw26I GTCTC 4 cut(s) 238, 531, 1188, 1490
Alw44I GTGCAC 1 cut(s) 388
AlwI GGATC 4 cut(s) 773, 835, 1096, 1208
Ama87I CYCGRG 1 cut(s) 957
Aor13HI TCCGGA 1 cut(s) 1390
AoxI GGCC 5 cut(s) 211, 293, 328, 1300, 1452
ApaLI GTGCAC 1 cut(s) 388
ApeKI GCWGC 2 cut(s) 308, 1112
ApoI RAATTY 1 cut(s) 584
ArsI GACNNNNNNTTYG 4 cut(s) 266, 298, 786, 818
Asp700I GAANNNNTTC 1 cut(s) 599
AspLEI GCGC 4 cut(s) 207, 372, 515, 1360
AspS9I GGNCC 4 cut(s) 101, 789, 1342, 1452
AsuC2I CCSGG 1 cut(s) 99
AsuHPI GGTGA 3 cut(s) 158, 200, 973
AvaI CYCGRG 1 cut(s) 957
AvaII GGWCC 3 cut(s) 101, 789, 1342
BaeGI GKGCMC 1 cut(s) 392
BalI TGGCCA 1 cut(s) 330
BanI GGYRCC 3 cut(s) 217, 638, 951
BarI GAAGNNNNNNTAC 2 cut(s) 1028, 1060
BbsI GAAGAC 1 cut(s) 1128
Bbv12I GWGCWC 2 cut(s) 392, 762
BbvI GCAGC 2 cut(s) 295, 1124
BceAI ACGGC 2 cut(s) 933, 1153
BcgI CGANNNNNNTGC 2 cut(s) 1293, 1327
BciT130I CCWGG 4 cut(s) 215, 605, 689, 1317
BcnI CCSGG 1 cut(s) 99
BcoDI GTCTC 4 cut(s) 238, 531, 1188, 1490
BfaI CTAG 7 cut(s) 173, 278, 291, 632, 1200, 1328, 1466
BfoI RGCGCY 1 cut(s) 516
BfuAI ACCTGC 1 cut(s) 210
BglII AGATCT 1 cut(s) 1258
BisI GCNGC 3 cut(s) 249, 309, 1113
BlsI GCNGC 3 cut(s) 250, 310, 1114
BmcAI AGTACT 1 cut(s) 1046
Bme1390I CCNGG 5 cut(s) 99, 215, 605, 689, 1317
Bme18I GGWCC 3 cut(s) 101, 789, 1342
BmeT110I CYCGRG 1 cut(s) 957
BmgBI CACGTC 3 cut(s) 546, 909, 1165
BmgT120I GGNCC 4 cut(s) 101, 789, 1342, 1452
BmiI GGNNCC 3 cut(s) 219, 640, 953
BmrFI CCNGG 5 cut(s) 99, 215, 605, 689, 1317
BmsI GCATC 2 cut(s) 637, 1333
BpiI GAAGAC 1 cut(s) 1128
BpmI CTGGAG 2 cut(s) 671, 959
Bpu10I CCTNAGC 1 cut(s) 208
BpuEI CTTGAG 1 cut(s) 228
BpuMI CCSGG 1 cut(s) 99
BsaI GGTCTC 1 cut(s) 1188
BsaJI CCNNGG 6 cut(s) 516, 688, 956, 1264, 1315, 1448
BsaWI WCCGGW 2 cut(s) 1231, 1390
Bsc4I CCNNNNNNNGG 6 cut(s) 122, 247, 357, 650, 1270, 1351
Bse118I RCCGGY 2 cut(s) 893, 1244
Bse1I ACTGG 1 cut(s) 1085
Bse3DI GCAATG 2 cut(s) 906, 1369
BseAI TCCGGA 1 cut(s) 1390
BseBI CCWGG 4 cut(s) 215, 605, 689, 1317
BseDI CCNNGG 6 cut(s) 516, 688, 956, 1264, 1315, 1448
BseGI GGATG 4 cut(s) 236, 781, 1024, 1081
BseLI CCNNNNNNNGG 6 cut(s) 122, 247, 357, 650, 1270, 1351
BseMI GCAATG 2 cut(s) 906, 1369
BseMII CTCAG 6 cut(s) 243, 418, 523, 785, 831, 1416
BseNI ACTGG 1 cut(s) 1085
BseRI GAGGAG 2 cut(s) 122, 1442
BseSI GKGCMC 1 cut(s) 392
BseXI GCAGC 2 cut(s) 295, 1124
BshFI GGCC 5 cut(s) 213, 295, 330, 1302, 1454
BshNI GGYRCC 3 cut(s) 217, 638, 951
BsiHKAI GWGCWC 2 cut(s) 392, 762
BsiHKCI CYCGRG 1 cut(s) 957
BsiSI CCGG 5 cut(s) 99, 894, 1232, 1245, 1391
BslFI GGGAC 1 cut(s) 1286
BslI CCNNNNNNNGG 6 cut(s) 122, 247, 357, 650, 1270, 1351
BsmAI GTCTC 4 cut(s) 238, 531, 1188, 1490
BsmBI CGTCTC 2 cut(s) 531, 1490
BsmFI GGGAC 1 cut(s) 1286
BsmI GAATGC 1 cut(s) 1326
BsnI GGCC 5 cut(s) 213, 295, 330, 1302, 1454
Bso31I GGTCTC 1 cut(s) 1188
BsoBI CYCGRG 1 cut(s) 957
Bsp1286I GDGCHC 2 cut(s) 392, 762
Bsp13I TCCGGA 1 cut(s) 1390
BspACI CCGC 2 cut(s) 248, 299
BspANI GGCC 5 cut(s) 213, 295, 330, 1302, 1454
BspCNI CTCAG 6 cut(s) 244, 417, 522, 784, 832, 1417
BspEI TCCGGA 1 cut(s) 1390
BspLI GGNNCC 3 cut(s) 219, 640, 953
BspMI ACCTGC 1 cut(s) 210
BspPI GGATC 4 cut(s) 773, 835, 1096, 1208
BspQI GCTCTTC 1 cut(s) 441
BspT107I GGYRCC 3 cut(s) 217, 638, 951
BspTNI GGTCTC 1 cut(s) 1188
BsrDI GCAATG 2 cut(s) 906, 1369
BsrFI RCCGGY 2 cut(s) 893, 1244
BsrI ACTGG 1 cut(s) 1085
BssAI RCCGGY 2 cut(s) 893, 1244
BssECI CCNNGG 6 cut(s) 516, 688, 956, 1264, 1315, 1448
BssT1I CCWWGG 2 cut(s) 516, 1448
Bst2UI CCWGG 4 cut(s) 215, 605, 689, 1317
Bst4CI ACNGT 4 cut(s) 188, 502, 1339, 1444
Bst6I CTCTTC 2 cut(s) 441, 1300
BstAPI GCANNNNNTGC 2 cut(s) 387, 1366
BstC8I GCNNGC 3 cut(s) 30, 51, 637
BstDEI CTNAG 8 cut(s) 208, 252, 404, 509, 699, 771, 840, 1425
BstDSI CCRYGG 1 cut(s) 1264
BstF5I GGATG 4 cut(s) 236, 781, 1024, 1081
BstH2I RGCGCY 1 cut(s) 516
BstHHI GCGC 4 cut(s) 207, 372, 515, 1360
BstMAI GTCTC 4 cut(s) 238, 531, 1188, 1490
BstMWI GCNNNNNNNGC 3 cut(s) 378, 387, 1366
BstNI CCWGG 4 cut(s) 215, 605, 689, 1317
BstNSI RCATGY 1 cut(s) 1112
BstSCI CCNGG 5 cut(s) 97, 213, 603, 687, 1315
BstSLI GKGCMC 1 cut(s) 392
BstV1I GCAGC 2 cut(s) 295, 1124
BstV2I GAAGAC 1 cut(s) 1128
BstX2I RGATCY 1 cut(s) 1258
BstXI CCANNNNNNTGG 1 cut(s) 880
BstYI RGATCY 1 cut(s) 1258
BsuRI GGCC 5 cut(s) 213, 295, 330, 1302, 1454
BtgI CCRYGG 1 cut(s) 1264
BtrI CACGTC 3 cut(s) 546, 909, 1165
BtsCI GGATG 4 cut(s) 236, 781, 1024, 1081
BveI ACCTGC 1 cut(s) 210
Cac8I GCNNGC 3 cut(s) 30, 51, 637
CfoI GCGC 4 cut(s) 207, 372, 515, 1360
Cfr10I RCCGGY 2 cut(s) 893, 1244
Cfr13I GGNCC 4 cut(s) 101, 789, 1342, 1452
Csp6I GTAC 4 cut(s) 44, 571, 844, 1045
CviQI GTAC 4 cut(s) 44, 571, 844, 1045
DdeI CTNAG 8 cut(s) 208, 252, 404, 509, 699, 771, 840, 1425
EaeI YGGCCR 2 cut(s) 328, 1300
Eam1104I CTCTTC 2 cut(s) 441, 1300
EarI CTCTTC 2 cut(s) 441, 1300
Eco130I CCWWGG 2 cut(s) 516, 1448
Eco147I AGGCCT 1 cut(s) 213
Eco31I GGTCTC 1 cut(s) 1188
Eco47I GGWCC 3 cut(s) 101, 789, 1342
Eco88I CYCGRG 1 cut(s) 957
EcoRII CCWGG 4 cut(s) 213, 603, 687, 1315
EcoT14I CCWWGG 2 cut(s) 516, 1448
EcoT22I ATGCAT 3 cut(s) 630, 1326, 1371
ErhI CCWWGG 2 cut(s) 516, 1448
Esp3I CGTCTC 2 cut(s) 531, 1490
FalI AAGNNNNNCTT 2 cut(s) 435, 467
FaqI GGGAC 1 cut(s) 1286
Fnu4HI GCNGC 3 cut(s) 249, 309, 1113
FokI GGATG 4 cut(s) 223, 788, 1031, 1068
Fsp4HI GCNGC 3 cut(s) 249, 309, 1113
FspBI CTAG 7 cut(s) 173, 278, 291, 632, 1200, 1328, 1466
GlaI GCGC 4 cut(s) 206, 371, 514, 1359
GluI GCNGC 3 cut(s) 249, 309, 1113
GsuI CTGGAG 2 cut(s) 671, 959
HaeII RGCGCY 1 cut(s) 516
HaeIII GGCC 5 cut(s) 213, 295, 330, 1302, 1454
HapII CCGG 5 cut(s) 99, 894, 1232, 1245, 1391
HhaI GCGC 4 cut(s) 207, 372, 515, 1360
Hin6I GCGC 4 cut(s) 205, 370, 513, 1358
HinP1I GCGC 4 cut(s) 205, 370, 513, 1358
HincII GTYRAC 2 cut(s) 1135, 1335
HindII GTYRAC 2 cut(s) 1135, 1335
HinfI GANTC 4 cut(s) 613, 767, 836, 1022
HpaI GTTAAC 2 cut(s) 1135, 1335
HpaII CCGG 5 cut(s) 99, 894, 1232, 1245, 1391
HphI GGTGA 3 cut(s) 158, 200, 973
Hpy166II GTNNAC 5 cut(s) 390, 930, 1135, 1268, 1335
Hpy188I TCNGA 6 cut(s) 125, 476, 841, 940, 1027, 1490
Hpy188III TCNNGA 7 cut(s) 617, 773, 782, 881, 976, 1217, 1391
Hpy8I GTNNAC 5 cut(s) 390, 930, 1135, 1268, 1335
HpyAV CCTTC 6 cut(s) 589, 671, 703, 1057, 1295, 1355
HpyCH4III ACNGT 4 cut(s) 188, 502, 1339, 1444
HpyCH4IV ACGT 3 cut(s) 545, 908, 1164
HpyF10VI GCNNNNNNNGC 3 cut(s) 378, 387, 1366
HpyF3I CTNAG 8 cut(s) 208, 252, 404, 509, 699, 771, 840, 1425
HpySE526I ACGT 3 cut(s) 545, 908, 1164
HspAI GCGC 4 cut(s) 205, 370, 513, 1358
Kpn2I TCCGGA 1 cut(s) 1390
KspAI GTTAAC 2 cut(s) 1135, 1335
LguI GCTCTTC 1 cut(s) 441
LmnI GCTCC 3 cut(s) 679, 863, 1096
Lsp1109I GCAGC 2 cut(s) 295, 1124
LweI GCATC 2 cut(s) 637, 1333
MaeI CTAG 7 cut(s) 173, 278, 291, 632, 1200, 1328, 1466
MaeII ACGT 3 cut(s) 545, 908, 1164
MaeIII GTNAC 1 cut(s) 188
MboII GAAGA 8 cut(s) 91, 155, 458, 757, 979, 1001, 1133, 1317
MfeI CAATTG 2 cut(s) 18, 921
MflI RGATCY 1 cut(s) 1258
MhlI GDGCHC 2 cut(s) 392, 762
MlsI TGGCCA 1 cut(s) 330
MluNI TGGCCA 1 cut(s) 330
MmeI TCCRAC 2 cut(s) 1303, 1395
Mox20I TGGCCA 1 cut(s) 330
Mph1103I ATGCAT 3 cut(s) 630, 1326, 1371
MroI TCCGGA 1 cut(s) 1390
MroXI GAANNNNTTC 1 cut(s) 599
MscI TGGCCA 1 cut(s) 330
MseI TTAA 6 cut(s) 264, 465, 695, 1134, 1334, 1377
MslI CAYNNNNRTG 1 cut(s) 492
Msp20I TGGCCA 1 cut(s) 330
MspI CCGG 5 cut(s) 99, 894, 1232, 1245, 1391
MspR9I CCNGG 5 cut(s) 99, 215, 605, 689, 1317
MunI CAATTG 2 cut(s) 18, 921
Mva1269I GAATGC 1 cut(s) 1326
MvaI CCWGG 4 cut(s) 215, 605, 689, 1317
MwoI GCNNNNNNNGC 3 cut(s) 378, 387, 1366
NciI CCSGG 1 cut(s) 99
NlaIV GGNNCC 3 cut(s) 219, 640, 953
NmeAIII GCCGAG 1 cut(s) 376
NmuCI GTSAC 1 cut(s) 188
NsiI ATGCAT 3 cut(s) 630, 1326, 1371
NspI RCATGY 1 cut(s) 1112
PaqCI CACCTGC 1 cut(s) 210
PceI AGGCCT 1 cut(s) 213
PciSI GCTCTTC 1 cut(s) 441
PcsI WCGNNNNNNNCGW 1 cut(s) 762
PctI GAATGC 1 cut(s) 1326
PdmI GAANNNNTTC 1 cut(s) 599
PfeI GAWTC 4 cut(s) 613, 767, 836, 1022
PflFI GACNNNGTC 1 cut(s) 800
PkrI GCNGC 3 cut(s) 250, 310, 1114
Psp6I CCWGG 4 cut(s) 213, 603, 687, 1315
PspGI CCWGG 4 cut(s) 213, 603, 687, 1315
PspN4I GGNNCC 3 cut(s) 219, 640, 953
PspPI GGNCC 4 cut(s) 101, 789, 1342, 1452
PsuI RGATCY 1 cut(s) 1258
PsyI GACNNNGTC 1 cut(s) 800
RsaI GTAC 4 cut(s) 45, 572, 845, 1046
RsaNI GTAC 4 cut(s) 44, 571, 844, 1045
RseI CAYNNNNRTG 1 cut(s) 492
SapI GCTCTTC 1 cut(s) 441
SaqAI TTAA 6 cut(s) 264, 465, 695, 1134, 1334, 1377
SatI GCNGC 3 cut(s) 249, 309, 1113
Sau96I GGNCC 4 cut(s) 101, 789, 1342, 1452
ScaI AGTACT 1 cut(s) 1046
ScrFI CCNGG 5 cut(s) 99, 215, 605, 689, 1317
SduI GDGCHC 2 cut(s) 392, 762
SfaNI GCATC 2 cut(s) 637, 1333
SinI GGWCC 3 cut(s) 101, 789, 1342
SmiMI CAYNNNNRTG 1 cut(s) 492
SmlI CTYRAG 1 cut(s) 243
SmoI CTYRAG 1 cut(s) 243
SseBI AGGCCT 1 cut(s) 213
SsiI CCGC 2 cut(s) 248, 299
SspI AATATT 1 cut(s) 1177
SspMI CTAG 7 cut(s) 173, 278, 291, 632, 1200, 1328, 1466
StuI AGGCCT 1 cut(s) 213
StyD4I CCNGG 5 cut(s) 97, 213, 603, 687, 1315
StyI CCWWGG 2 cut(s) 516, 1448
TaaI ACNGT 4 cut(s) 188, 502, 1339, 1444
TaiI ACGT 3 cut(s) 548, 911, 1167
TaqI TCGA 3 cut(s) 159, 765, 804
TatI WGTACW 4 cut(s) 43, 570, 843, 1044
TauI GCSGC 1 cut(s) 251
TfiI GAWTC 4 cut(s) 613, 767, 836, 1022
Tru1I TTAA 6 cut(s) 264, 465, 695, 1134, 1334, 1377
Tru9I TTAA 6 cut(s) 264, 465, 695, 1134, 1334, 1377
TseFI GTSAC 1 cut(s) 188
TseI GCWGC 2 cut(s) 308, 1112
Tsp45I GTSAC 1 cut(s) 188
TspDTI ATGAA 7 cut(s) 318, 451, 722, 811, 980, 1035, 1216
TspGWI ACGGA 2 cut(s) 1253, 1278
Tth111I GACNNNGTC 1 cut(s) 800
VneI GTGCAC 1 cut(s) 388
VpaK11BI GGWCC 3 cut(s) 101, 789, 1342
XapI RAATTY 1 cut(s) 584
XceI RCATGY 1 cut(s) 1112
XmnI GAANNNNTTC 1 cut(s) 599
XspI CTAG 7 cut(s) 173, 278, 291, 632, 1200, 1328, 1466
ZrmI AGTACT 1 cut(s) 1046
Zsp2I ATGCAT 3 cut(s) 630, 1326, 1371
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.