Rh5AG289700

Cytochrome p450

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0000398
Physical Location & Seq
Reverse (-)
39607506 .. 39625000
17495 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG289700.1

Sequence Viewer

Length: 842 bp
ATGATGGTTTGGATTTGGGCAGTAATAATTGCATCGCTTGCTGTTGTTTATGTTCTGGAACCATGGAGAAGCAAGAAGAAGAGGTTACCTCCTGGTCCAAGAGGGTTTCCTATTTTCGGAAGCCTCAACTTGTTAGGGGAGTTTCCTTACAAAGACCTACACGGACTGGCTCAAAAGTATGGTGACATCATGCACATGCGTTTAGGCCTCGTGTCTGCCATCGTCGTCTCCTCCCCTCAAGCTGCTGAGCTCTTCCTCAAGACCCACGACCTTGTTTTCGCAAGCAGGCCACCTCACGAAGGCGCAAAGCACATCTCTTTCGGGCAGAGGAACTTGAGCTTTTCCAAGTATGGCTCTTATTGGCGAGACATGCGCAAGATGTGCACCCTCGAGTTGCTCAGCAACCACAAAATCAATACTTACAAGGAAATGAGGAGAGAAGAGGTTGCTCTTTTCATAGAGGCCATTAAAGAGGCAGCTGCAACCAGCCACGAACCAGTCAATCTCAGCGCTAAGGTGATTGACTTACCAAATCAACTTTCTGTGGGCTATCTATGGAGAATAATATTCACAGACCTACATCTCTCAAGCGGCGCAACAAAAAATTATATGATGAAGTACTTCAGAGAGGATCTGCATAGGCAGCTTCTTAGTATAGATTTTAAGAAGCAAGTCGATGGGCTTGAGATGCTGCAGATGAGCCAAGTAGCAAACCCTGGCATACCTAGCCACCCAAGAGCCGCCGCCCTCAGCAGGACCTCCCTCACCACTGCCGCCATAGCCTTTCCCATCGCAGCCGCAATCCAGCATAGGAGAGAGATCACCATCACCACCGTCACAGT

Protein Analysis

280

Amino Acids

31.67

Weight (kDa)

9.73

Isoelectric Point (pI)

38.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 30 - 169 1.7e-19 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000398)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g21960 FvH4_3g24140 FvH4_3g24150 FvH4_7g09230 FvH4_7g09260
malus_domestica MD00G1169700.v1.1 MD00G1169800.v1.1 MD01G1016400.v1.1 MD03G1188000.v1.1 MD03G1188100.v1.1 MD11G1219700.v1.1 MD11G1219900.v1.1 MD11G1220200.v1.1 MD11G1220400.v1.1 MD11G1220600.v1.1
prunus_persica Prupe.2G006500_v2.0.a1 Prupe.4G235500_v2.0.a1 Prupe.4G235600_v2.0.a1 Prupe.4G235700_v2.0.a1 Prupe.4G235700_v2.0.a1 Prupe.4G235800_v2.0.a1 Prupe.4G235900_v2.0.a1 Prupe.6G176500_v2.0.a1
pyrus_communis pycom01g04750 pycom03g14060 pycom11g19360 pycom11g19370 pycom11g19380 pycom11g19390 pycom11g19400 pycom11g19460
rosa_chinensis RchiOBHm_Chr1g0345321 RchiOBHm_Chr1g0345511 RchiOBHm_Chr1g0345781 RchiOBHm_Chr2g0115981 RchiOBHm_Chr5g0043151 RchiOBHm_Chr5g0043161 RchiOBHm_Chr5g0043901 RchiOBHm_Chr6g0274841
rosa_laevigata RLG00000013516 RLG00000018229 RLG00000028840 RLG00000028923 RLG00000034169 RLG00000034223
rosa_multiflora Rmu_co8474237.1_g000001 Rmu_sc0000698.1_g000004 Rmu_sc0000698.1_g000021 Rmu_sc0000698.1_g000023 Rmu_sc0000698.1_g000056 Rmu_sc0000698.1_g000057 Rmu_sc0000698.1_g000122 Rmu_sc0000698.1_g000123 Rmu_sc0000698.1_g000124 Rmu_sc0001292.1_g000005 Rmu_sc0001292.1_g000006 Rmu_sc0001349.1_g000002 Rmu_sc0001692.1_g000035 Rmu_sc0002079.1_g000035 Rmu_sc0002079.1_g000045 Rmu_sc0002329.1_g000017 Rmu_sc0008148.1_g000056 Rmu_sc0008148.1_g000057 Rmu_sc0008148.1_g000058 Rmu_sc0008148.1_g000062 Rmu_sc0008611.1_g000010 Rmu_sc0009227.1_g000007
rosa_roxburghii Rroxscaffold_1G00011260 Rroxscaffold_1G00037590 Rroxscaffold_2G00127610 Rroxscaffold_4G00309120 Rroxscaffold_7G00193880
rosa_rugosa Rorug01G0177400 Rorug02G0202400 Rorug05G0204600 Rorug05G0209000 Rorug06G0085100 Rorug06G0085200
rosa_samantha Rh1BG154400 Rh1DG186600 Rh2CG265200 Rh2DG267000 Rh5AG289700 Rh5AG289800 Rh5AG290000 Rh5AG293200 Rh5CG326500 Rh5DG305700 Rh5DG305800 Rh5DG310100 Rh6BG204200 Rh6CG204500 Rh6DG195700
rosa_wichuraiana Rw1G016100 Rw1G016470 Rw2G020290 Rw5G026830 Rw5G026910 Rw5G026920 Rw5G026930 Rw6G017460

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 374
AciI CCGC 5 cut(s) 591, 741, 744, 774, 798
AclWI GGATC 1 cut(s) 639
AcuI CTGAAG 1 cut(s) 607
AfaI GTAC 1 cut(s) 620
AfeI AGCGCT 1 cut(s) 511
AfiI CCNNNNNNNGG 3 cut(s) 116, 299, 753
AjnI CCWGG 2 cut(s) 91, 715
AjuI GAANNNNNNNTTGG 2 cut(s) 523, 555
AluBI AGCT 5 cut(s) 242, 250, 339, 479, 646
AluI AGCT 5 cut(s) 242, 250, 339, 479, 646
Alw21I GWGCWC 2 cut(s) 252, 386
Alw26I GTCTC 2 cut(s) 232, 360
Alw44I GTGCAC 1 cut(s) 382
AlwI GGATC 1 cut(s) 639
Ama87I CYCGRG 1 cut(s) 389
Aor51HI AGCGCT 1 cut(s) 511
AoxI GGCC 3 cut(s) 205, 287, 462
ApaLI GTGCAC 1 cut(s) 382
ApeKI GCWGC 6 cut(s) 242, 476, 479, 643, 691, 794
ArsI GACNNNNNNTTYG 2 cut(s) 260, 292
Asp700I GAANNNNTTC 1 cut(s) 620
AspLEI GCGC 4 cut(s) 305, 375, 512, 596
AspS9I GGNCC 2 cut(s) 95, 756
AsuHPI GGTGA 5 cut(s) 194, 529, 757, 814, 820
AvaI CYCGRG 1 cut(s) 389
AvaII GGWCC 2 cut(s) 95, 756
BaeGI GKGCMC 1 cut(s) 386
BanII GRGCYC 1 cut(s) 252
BauI CACGAG 1 cut(s) 209
Bbv12I GWGCWC 2 cut(s) 252, 386
BbvCI CCTCAGC 1 cut(s) 749
BbvI GCAGC 6 cut(s) 229, 466, 488, 655, 678, 806
BccI CCATC 4 cut(s) 227, 671, 797, 833
BciT130I CCWGG 2 cut(s) 93, 717
BcoDI GTCTC 2 cut(s) 232, 360
BfaI CTAG 1 cut(s) 726
BfmI CTRYAG 1 cut(s) 692
BfoI RGCGCY 1 cut(s) 513
BlpI GCTNAGC 2 cut(s) 246, 398
BmcAI AGTACT 1 cut(s) 620
Bme1390I CCNGG 2 cut(s) 93, 717
Bme18I GGWCC 2 cut(s) 95, 756
BmeT110I CYCGRG 1 cut(s) 389
BmgT120I GGNCC 2 cut(s) 95, 756
BmiI GGNNCC 1 cut(s) 60
BmrFI CCNGG 2 cut(s) 93, 717
BmsI GCATC 2 cut(s) 41, 678
BplI GAGNNNNNCTC 2 cut(s) 73, 105
Bpu10I CCTNAGC 2 cut(s) 513, 749
Bpu1102I GCTNAGC 2 cut(s) 246, 398
BpuEI CTTGAG 5 cut(s) 222, 242, 355, 571, 704
BsaBI GATNNNNATC 1 cut(s) 824
BsaJI CCNNGG 2 cut(s) 62, 715
Bsc4I CCNNNNNNNGG 3 cut(s) 116, 299, 753
Bse1I ACTGG 2 cut(s) 171, 497
Bse8I GATNNNNATC 1 cut(s) 824
BseBI CCWGG 2 cut(s) 93, 717
BseDI CCNNGG 2 cut(s) 62, 715
BseJI GATNNNNATC 1 cut(s) 824
BseLI CCNNNNNNNGG 3 cut(s) 116, 299, 753
BseMII CTCAG 4 cut(s) 237, 412, 520, 763
BseNI ACTGG 2 cut(s) 171, 497
BseRI GAGGAG 2 cut(s) 220, 448
BseSI GKGCMC 1 cut(s) 386
BseXI GCAGC 6 cut(s) 229, 466, 488, 655, 678, 806
BshFI GGCC 3 cut(s) 207, 289, 464
BsiHKAI GWGCWC 2 cut(s) 252, 386
BsiHKCI CYCGRG 1 cut(s) 389
BslI CCNNNNNNNGG 3 cut(s) 116, 299, 753
BsmAI GTCTC 2 cut(s) 232, 360
BsmBI CGTCTC 1 cut(s) 232
BsnI GGCC 3 cut(s) 207, 289, 464
BsoBI CYCGRG 1 cut(s) 389
Bsp1286I GDGCHC 2 cut(s) 252, 386
Bsp143I GATC 2 cut(s) 631, 819
Bsp1720I GCTNAGC 2 cut(s) 246, 398
Bsp19I CCATGG 1 cut(s) 62
BspACI CCGC 5 cut(s) 591, 741, 744, 774, 798
BspANI GGCC 3 cut(s) 207, 289, 464
BspCNI CTCAG 4 cut(s) 238, 411, 519, 762
BspLI GGNNCC 1 cut(s) 60
BspMAI CTGCAG 1 cut(s) 696
BspPI GGATC 1 cut(s) 639
BspQI GCTCTTC 1 cut(s) 257
BsrI ACTGG 2 cut(s) 171, 497
BssECI CCNNGG 2 cut(s) 62, 715
BssMI GATC 2 cut(s) 631, 819
BssSI CACGAG 1 cut(s) 209
BssT1I CCWWGG 1 cut(s) 62
Bst2BI CACGAG 1 cut(s) 209
Bst2UI CCWGG 2 cut(s) 93, 717
Bst4CI ACNGT 2 cut(s) 835, 841
Bst6I CTCTTC 3 cut(s) 74, 257, 435
BstAPI GCANNNNNTGC 2 cut(s) 38, 381
BstC8I GCNNGC 3 cut(s) 39, 283, 287
BstDEI CTNAG 6 cut(s) 246, 398, 506, 513, 650, 749
BstDSI CCRYGG 1 cut(s) 62
BstEII GGTNACC 1 cut(s) 84
BstENI CCTNNNNNAGG 1 cut(s) 297
BstH2I RGCGCY 1 cut(s) 513
BstHHI GCGC 4 cut(s) 305, 375, 512, 596
BstKTI GATC 2 cut(s) 634, 822
BstMAI GTCTC 2 cut(s) 232, 360
BstMBI GATC 2 cut(s) 631, 819
BstMWI GCNNNNNNNGC 7 cut(s) 38, 370, 381, 643, 688, 726, 779
BstNI CCWGG 2 cut(s) 93, 717
BstNSI RCATGY 2 cut(s) 199, 373
BstPI GGTNACC 1 cut(s) 84
BstSCI CCNGG 2 cut(s) 91, 715
BstSFI CTRYAG 1 cut(s) 692
BstSLI GKGCMC 1 cut(s) 386
BstV1I GCAGC 6 cut(s) 229, 466, 488, 655, 678, 806
BstX2I RGATCY 1 cut(s) 631
BstYI RGATCY 1 cut(s) 631
BsuRI GGCC 3 cut(s) 207, 289, 464
BtgI CCRYGG 1 cut(s) 62
BtgZI GCGATG 2 cut(s) 18, 775
BtsI GCAGTG 1 cut(s) 768
BtsIMutI CAGTG 1 cut(s) 768
Cac8I GCNNGC 3 cut(s) 39, 283, 287
CfoI GCGC 4 cut(s) 305, 375, 512, 596
Cfr13I GGNCC 2 cut(s) 95, 756
Csp6I GTAC 1 cut(s) 619
CviAII CATG 4 cut(s) 63, 190, 196, 370
CviQI GTAC 1 cut(s) 619
DdeI CTNAG 6 cut(s) 246, 398, 506, 513, 650, 749
DpnI GATC 2 cut(s) 633, 821
DpnII GATC 2 cut(s) 631, 819
Eam1104I CTCTTC 3 cut(s) 74, 257, 435
EarI CTCTTC 3 cut(s) 74, 257, 435
Ecl136II GAGCTC 1 cut(s) 250
Eco130I CCWWGG 1 cut(s) 62
Eco147I AGGCCT 1 cut(s) 207
Eco24I GRGCYC 1 cut(s) 252
Eco47I GGWCC 2 cut(s) 95, 756
Eco47III AGCGCT 1 cut(s) 511
Eco53kI GAGCTC 1 cut(s) 250
Eco57I CTGAAG 1 cut(s) 607
Eco88I CYCGRG 1 cut(s) 389
Eco91I GGTNACC 1 cut(s) 84
EcoICRI GAGCTC 1 cut(s) 250
EcoNI CCTNNNNNAGG 1 cut(s) 297
EcoO109I RGGNCCY 1 cut(s) 756
EcoO65I GGTNACC 1 cut(s) 84
EcoRII CCWGG 2 cut(s) 91, 715
EcoT14I CCWWGG 1 cut(s) 62
EcoT38I GRGCYC 1 cut(s) 252
ErhI CCWWGG 1 cut(s) 62
Esp3I CGTCTC 1 cut(s) 232
FaeI CATG 4 cut(s) 66, 193, 199, 373
FatI CATG 4 cut(s) 62, 189, 195, 369
FriOI GRGCYC 1 cut(s) 252
FspBI CTAG 1 cut(s) 726
FspI TGCGCA 1 cut(s) 374
GlaI GCGC 4 cut(s) 304, 374, 511, 595
HaeII RGCGCY 1 cut(s) 513
HaeIII GGCC 3 cut(s) 207, 289, 464
HhaI GCGC 4 cut(s) 305, 375, 512, 596
Hin1II CATG 4 cut(s) 66, 193, 199, 373
Hin6I GCGC 4 cut(s) 303, 373, 510, 594
HinP1I GCGC 4 cut(s) 303, 373, 510, 594
HphI GGTGA 5 cut(s) 194, 529, 757, 814, 820
Hpy166II GTNNAC 1 cut(s) 384
Hpy188I TCNGA 2 cut(s) 119, 626
Hpy188III TCNNGA 3 cut(s) 56, 259, 296
Hpy8I GTNNAC 1 cut(s) 384
Hpy99I CGWCG 1 cut(s) 227
HpyAV CCTTC 1 cut(s) 293
HpyCH4III ACNGT 2 cut(s) 835, 841
HpyCH4V TGCA 6 cut(s) 32, 193, 384, 482, 637, 694
HpyF10VI GCNNNNNNNGC 7 cut(s) 38, 370, 381, 643, 688, 726, 779
HpyF3I CTNAG 6 cut(s) 246, 398, 506, 513, 650, 749
Hsp92II CATG 4 cut(s) 66, 193, 199, 373
HspAI GCGC 4 cut(s) 303, 373, 510, 594
Kzo9I GATC 2 cut(s) 631, 819
LguI GCTCTTC 1 cut(s) 257
Lsp1109I GCAGC 6 cut(s) 229, 466, 488, 655, 678, 806
LweI GCATC 2 cut(s) 41, 678
MaeI CTAG 1 cut(s) 726
MaeIII GTNAC 3 cut(s) 84, 182, 835
MalI GATC 2 cut(s) 633, 821
MboI GATC 2 cut(s) 631, 819
MboII GAAGA 4 cut(s) 88, 91, 244, 452
MflI RGATCY 1 cut(s) 631
MhlI GDGCHC 2 cut(s) 252, 386
MluCI AATT 2 cut(s) 27, 604
MroXI GAANNNNTTC 1 cut(s) 620
MseI TTAA 2 cut(s) 468, 663
MslI CAYNNNNRTG 1 cut(s) 194
MspA1I CMGCKG 1 cut(s) 479
MspR9I CCNGG 2 cut(s) 93, 717
MvaI CCWGG 2 cut(s) 93, 717
MwoI GCNNNNNNNGC 7 cut(s) 38, 370, 381, 643, 688, 726, 779
NcoI CCATGG 1 cut(s) 62
NdeII GATC 2 cut(s) 631, 819
NlaIII CATG 4 cut(s) 66, 193, 199, 373
NlaIV GGNNCC 1 cut(s) 60
NmuCI GTSAC 2 cut(s) 182, 835
NsbI TGCGCA 1 cut(s) 374
NspI RCATGY 2 cut(s) 199, 373
PaeR7I CTCGAG 1 cut(s) 389
PceI AGGCCT 1 cut(s) 207
PciSI GCTCTTC 1 cut(s) 257
PdmI GAANNNNTTC 1 cut(s) 620
PpuMI RGGWCCY 1 cut(s) 756
Psp124BI GAGCTC 1 cut(s) 252
Psp5II RGGWCCY 1 cut(s) 756
Psp6I CCWGG 2 cut(s) 91, 715
PspEI GGTNACC 1 cut(s) 84
PspGI CCWGG 2 cut(s) 91, 715
PspN4I GGNNCC 1 cut(s) 60
PspPI GGNCC 2 cut(s) 95, 756
PspPPI RGGWCCY 1 cut(s) 756
PspXI VCTCGAGB 1 cut(s) 389
PstI CTGCAG 1 cut(s) 696
PsuI RGATCY 1 cut(s) 631
PvuII CAGCTG 1 cut(s) 479
RsaI GTAC 1 cut(s) 620
RsaNI GTAC 1 cut(s) 619
RseI CAYNNNNRTG 1 cut(s) 194
SacI GAGCTC 1 cut(s) 252
SapI GCTCTTC 1 cut(s) 257
SaqAI TTAA 2 cut(s) 468, 663
Sau3AI GATC 2 cut(s) 631, 819
Sau96I GGNCC 2 cut(s) 95, 756
ScaI AGTACT 1 cut(s) 620
ScrFI CCNGG 2 cut(s) 93, 717
SduI GDGCHC 2 cut(s) 252, 386
SfaNI GCATC 2 cut(s) 41, 678
SfcI CTRYAG 1 cut(s) 692
Sfr274I CTCGAG 1 cut(s) 389
SinI GGWCC 2 cut(s) 95, 756
SlaI CTCGAG 1 cut(s) 389
SmiMI CAYNNNNRTG 1 cut(s) 194
SmlI CTYRAG 6 cut(s) 237, 257, 334, 389, 586, 683
SmoI CTYRAG 6 cut(s) 237, 257, 334, 389, 586, 683
Sse9I AATT 2 cut(s) 27, 604
SseBI AGGCCT 1 cut(s) 207
SsiI CCGC 5 cut(s) 591, 741, 744, 774, 798
SspI AATATT 1 cut(s) 567
SspMI CTAG 1 cut(s) 726
SstI GAGCTC 1 cut(s) 252
StuI AGGCCT 1 cut(s) 207
StyD4I CCNGG 2 cut(s) 91, 715
StyI CCWWGG 1 cut(s) 62
TaaI ACNGT 2 cut(s) 835, 841
TaqI TCGA 2 cut(s) 390, 675
TasI AATT 2 cut(s) 27, 604
TatI WGTACW 1 cut(s) 618
TauI GCSGC 5 cut(s) 594, 743, 746, 776, 800
Tru1I TTAA 2 cut(s) 468, 663
Tru9I TTAA 2 cut(s) 468, 663
TscAI CASTG 1 cut(s) 775
TseFI GTSAC 2 cut(s) 182, 835
TseI GCWGC 6 cut(s) 242, 476, 479, 643, 691, 794
Tsp45I GTSAC 2 cut(s) 182, 835
TspDTI ATGAA 2 cut(s) 445, 629
TspGWI ACGGA 1 cut(s) 177
TspRI CASTG 1 cut(s) 775
VneI GTGCAC 1 cut(s) 382
VpaK11BI GGWCC 2 cut(s) 95, 756
XagI CCTNNNNNAGG 1 cut(s) 297
XceI RCATGY 2 cut(s) 199, 373
XhoI CTCGAG 1 cut(s) 389
XmnI GAANNNNTTC 1 cut(s) 620
XspI CTAG 1 cut(s) 726
ZrmI AGTACT 1 cut(s) 620
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.