Cytochrome p450
Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc36I | ACCTGC | 1 cut(s) | 699 |
| Acc65I | GGTACC | 1 cut(s) | 1093 |
| AccB1I | GGYRCC | 1 cut(s) | 1093 |
| AccBSI | CCGCTC | 1 cut(s) | 251 |
| AccI | GTMKAC | 1 cut(s) | 295 |
| AccII | CGCG | 4 cut(s) | 375, 861, 896, 1165 |
| AciI | CCGC | 5 cut(s) | 208, 251, 614, 683, 896 |
| AclI | AACGTT | 1 cut(s) | 228 |
| AclWI | GGATC | 2 cut(s) | 171, 1211 |
| AcoI | YGGCCR | 1 cut(s) | 252 |
| AcsI | RAATTY | 2 cut(s) | 5, 1159 |
| AcuI | CTGAAG | 2 cut(s) | 855, 1475 |
| AfaI | GTAC | 6 cut(s) | 203, 578, 1049, 1095, 1233, 1290 |
| AfiI | CCNNNNNNNGG | 5 cut(s) | 125, 160, 250, 308, 883 |
| AflIII | ACRYGT | 3 cut(s) | 859, 888, 1401 |
| AgsI | TTSAA | 5 cut(s) | 433, 470, 479, 658, 773 |
| AjnI | CCWGG | 1 cut(s) | 100 |
| AleI | CACNNNNGTG | 1 cut(s) | 614 |
| AluBI | AGCT | 4 cut(s) | 259, 348, 988, 1285 |
| AluI | AGCT | 4 cut(s) | 259, 348, 988, 1285 |
| Alw26I | GTCTC | 2 cut(s) | 1010, 1191 |
| AlwI | GGATC | 2 cut(s) | 171, 1211 |
| AoxI | GGCC | 6 cut(s) | 214, 252, 435, 820, 873, 897 |
| ApeKI | GCWGC | 3 cut(s) | 129, 644, 1251 |
| ApoI | RAATTY | 2 cut(s) | 5, 1159 |
| ArsI | GACNNNNNNTTYG | 6 cut(s) | 269, 301, 575, 607, 1447, 1479 |
| Asp718I | GGTACC | 1 cut(s) | 1093 |
| AspLEI | GCGC | 4 cut(s) | 375, 682, 1167, 1319 |
| AspS9I | GGNCC | 5 cut(s) | 104, 151, 820, 874, 1455 |
| AsuHPI | GGTGA | 2 cut(s) | 628, 694 |
| AvaII | GGWCC | 3 cut(s) | 104, 151, 1455 |
| AxyI | CCTNAGG | 1 cut(s) | 950 |
| BaeI | ACNNNNGTAYC | 2 cut(s) | 1085, 1118 |
| BanI | GGYRCC | 1 cut(s) | 1093 |
| BanII | GRGCYC | 1 cut(s) | 702 |
| BauI | CACGAG | 1 cut(s) | 246 |
| BbsI | GAAGAC | 2 cut(s) | 722, 1315 |
| BbvI | GCAGC | 3 cut(s) | 141, 656, 1263 |
| BccI | CCATC | 5 cut(s) | 553, 774, 922, 1127, 1133 |
| BceAI | ACGGC | 2 cut(s) | 205, 627 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 372, 406 |
| BciT130I | CCWGG | 1 cut(s) | 102 |
| BclI | TGATCA | 1 cut(s) | 661 |
| BcoDI | GTCTC | 2 cut(s) | 1010, 1191 |
| BfaI | CTAG | 4 cut(s) | 176, 212, 989, 1452 |
| BfuAI | ACCTGC | 1 cut(s) | 699 |
| BglII | AGATCT | 1 cut(s) | 525 |
| BisI | GCNGC | 7 cut(s) | 130, 209, 252, 645, 683, 897, 1252 |
| BlsI | GCNGC | 7 cut(s) | 131, 210, 253, 646, 684, 898, 1253 |
| BmcAI | AGTACT | 1 cut(s) | 1049 |
| Bme1390I | CCNGG | 1 cut(s) | 102 |
| Bme18I | GGWCC | 3 cut(s) | 104, 151, 1455 |
| BmgT120I | GGNCC | 5 cut(s) | 104, 151, 820, 874, 1455 |
| BmiI | GGNNCC | 3 cut(s) | 153, 504, 1095 |
| BmrFI | CCNGG | 1 cut(s) | 102 |
| BmsI | GCATC | 3 cut(s) | 81, 727, 964 |
| BpiI | GAAGAC | 2 cut(s) | 722, 1315 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 347, 379 |
| BpuEI | CTTGAG | 1 cut(s) | 1096 |
| BsaBI | GATNNNNATC | 1 cut(s) | 168 |
| BsaI | GGTCTC | 2 cut(s) | 1010, 1191 |
| BsaJI | CCNNGG | 2 cut(s) | 777, 1458 |
| Bsc4I | CCNNNNNNNGG | 5 cut(s) | 125, 160, 250, 308, 883 |
| Bse21I | CCTNAGG | 1 cut(s) | 950 |
| Bse8I | GATNNNNATC | 1 cut(s) | 168 |
| BseBI | CCWGG | 1 cut(s) | 102 |
| BseDI | CCNNGG | 2 cut(s) | 777, 1458 |
| BseGI | GGATG | 5 cut(s) | 48, 564, 787, 955, 1084 |
| BseJI | GATNNNNATC | 1 cut(s) | 168 |
| BseLI | CCNNNNNNNGG | 5 cut(s) | 125, 160, 250, 308, 883 |
| BseMII | CTCAG | 5 cut(s) | 345, 529, 915, 954, 1419 |
| BseRI | GAGGAG | 1 cut(s) | 457 |
| BseX3I | CGGCCG | 1 cut(s) | 252 |
| BseXI | GCAGC | 3 cut(s) | 141, 656, 1263 |
| BseYI | CCCAGC | 1 cut(s) | 817 |
| Bsh1236I | CGCG | 4 cut(s) | 375, 861, 896, 1165 |
| Bsh1285I | CGRYCG | 1 cut(s) | 255 |
| BshFI | GGCC | 6 cut(s) | 216, 254, 437, 822, 875, 899 |
| BshNI | GGYRCC | 1 cut(s) | 1093 |
| BsiEI | CGRYCG | 1 cut(s) | 255 |
| BslFI | GGGAC | 2 cut(s) | 137, 226 |
| BslI | CCNNNNNNNGG | 5 cut(s) | 125, 160, 250, 308, 883 |
| BsmAI | GTCTC | 2 cut(s) | 1010, 1191 |
| BsmFI | GGGAC | 2 cut(s) | 137, 226 |
| BsmI | GAATGC | 1 cut(s) | 1329 |
| BsnI | GGCC | 6 cut(s) | 216, 254, 437, 822, 875, 899 |
| Bso31I | GGTCTC | 2 cut(s) | 1010, 1191 |
| Bsp1286I | GDGCHC | 1 cut(s) | 702 |
| Bsp1407I | TGTACA | 1 cut(s) | 201 |
| Bsp143I | GATC | 5 cut(s) | 163, 420, 525, 661, 1216 |
| BspACI | CCGC | 5 cut(s) | 208, 251, 614, 683, 896 |
| BspANI | GGCC | 6 cut(s) | 216, 254, 437, 822, 875, 899 |
| BspCNI | CTCAG | 5 cut(s) | 346, 528, 914, 953, 1420 |
| BspFNI | CGCG | 4 cut(s) | 375, 861, 896, 1165 |
| BspHI | TCATGA | 1 cut(s) | 304 |
| BspLI | GGNNCC | 3 cut(s) | 153, 504, 1095 |
| BspMI | ACCTGC | 1 cut(s) | 699 |
| BspPI | GGATC | 2 cut(s) | 171, 1211 |
| BspT107I | GGYRCC | 1 cut(s) | 1093 |
| BspTNI | GGTCTC | 2 cut(s) | 1010, 1191 |
| BsrBI | CCGCTC | 1 cut(s) | 251 |
| BsrGI | TGTACA | 1 cut(s) | 201 |
| BssECI | CCNNGG | 2 cut(s) | 777, 1458 |
| BssMI | GATC | 5 cut(s) | 163, 420, 525, 661, 1216 |
| BssSI | CACGAG | 1 cut(s) | 246 |
| BssT1I | CCWWGG | 2 cut(s) | 777, 1458 |
| Bst2BI | CACGAG | 1 cut(s) | 246 |
| Bst2UI | CCWGG | 1 cut(s) | 102 |
| Bst4CI | ACNGT | 4 cut(s) | 1135, 1293, 1342, 1350 |
| Bst6I | CTCTTC | 1 cut(s) | 465 |
| BstAUI | TGTACA | 1 cut(s) | 201 |
| BstC8I | GCNNGC | 3 cut(s) | 36, 395, 863 |
| BstDEI | CTNAG | 7 cut(s) | 354, 407, 515, 901, 940, 950, 1428 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 306 |
| BstF5I | GGATG | 5 cut(s) | 48, 564, 787, 955, 1084 |
| BstFNI | CGCG | 4 cut(s) | 375, 861, 896, 1165 |
| BstHHI | GCGC | 4 cut(s) | 375, 682, 1167, 1319 |
| BstKTI | GATC | 5 cut(s) | 166, 423, 528, 664, 1219 |
| BstMAI | GTCTC | 2 cut(s) | 1010, 1191 |
| BstMBI | GATC | 5 cut(s) | 163, 420, 525, 661, 1216 |
| BstMCI | CGRYCG | 1 cut(s) | 255 |
| BstMWI | GCNNNNNNNGC | 5 cut(s) | 379, 691, 737, 1282, 1333 |
| BstNI | CCWGG | 1 cut(s) | 102 |
| BstNSI | RCATGY | 5 cut(s) | 208, 397, 869, 892, 1405 |
| BstSCI | CCNGG | 1 cut(s) | 100 |
| BstUI | CGCG | 4 cut(s) | 375, 861, 896, 1165 |
| BstV1I | GCAGC | 3 cut(s) | 141, 656, 1263 |
| BstV2I | GAAGAC | 2 cut(s) | 722, 1315 |
| BstX2I | RGATCY | 3 cut(s) | 163, 525, 1216 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 1209 |
| BstYI | RGATCY | 3 cut(s) | 163, 525, 1216 |
| BstZI | CGGCCG | 1 cut(s) | 252 |
| Bsu36I | CCTNAGG | 1 cut(s) | 950 |
| BsuRI | GGCC | 6 cut(s) | 216, 254, 437, 822, 875, 899 |
| BtgZI | GCGATG | 1 cut(s) | 516 |
| BtsCI | GGATG | 5 cut(s) | 48, 564, 787, 955, 1084 |
| BtsIMutI | CAGTG | 3 cut(s) | 523, 909, 1131 |
| BveI | ACCTGC | 1 cut(s) | 699 |
| Cac8I | GCNNGC | 3 cut(s) | 36, 395, 863 |
| CciI | TCATGA | 1 cut(s) | 304 |
| CfoI | GCGC | 4 cut(s) | 375, 682, 1167, 1319 |
| Cfr13I | GGNCC | 5 cut(s) | 104, 151, 820, 874, 1455 |
| Csp6I | GTAC | 6 cut(s) | 202, 577, 1048, 1094, 1232, 1289 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 499, 534 |
| CviQI | GTAC | 6 cut(s) | 202, 577, 1048, 1094, 1232, 1289 |
| DdeI | CTNAG | 7 cut(s) | 354, 407, 515, 901, 940, 950, 1428 |
| DpnI | GATC | 5 cut(s) | 165, 422, 527, 663, 1218 |
| DpnII | GATC | 5 cut(s) | 163, 420, 525, 661, 1216 |
| EaeI | YGGCCR | 1 cut(s) | 252 |
| EagI | CGGCCG | 1 cut(s) | 252 |
| Eam1104I | CTCTTC | 1 cut(s) | 465 |
| EarI | CTCTTC | 1 cut(s) | 465 |
| EclXI | CGGCCG | 1 cut(s) | 252 |
| Eco130I | CCWWGG | 2 cut(s) | 777, 1458 |
| Eco147I | AGGCCT | 1 cut(s) | 216 |
| Eco24I | GRGCYC | 1 cut(s) | 702 |
| Eco31I | GGTCTC | 2 cut(s) | 1010, 1191 |
| Eco32I | GATATC | 1 cut(s) | 66 |
| Eco47I | GGWCC | 3 cut(s) | 104, 151, 1455 |
| Eco52I | CGGCCG | 1 cut(s) | 252 |
| Eco57I | CTGAAG | 2 cut(s) | 855, 1475 |
| Eco81I | CCTNAGG | 1 cut(s) | 950 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 306 |
| EcoO109I | RGGNCCY | 2 cut(s) | 151, 874 |
| EcoRII | CCWGG | 1 cut(s) | 100 |
| EcoRV | GATATC | 1 cut(s) | 66 |
| EcoT14I | CCWWGG | 2 cut(s) | 777, 1458 |
| EcoT38I | GRGCYC | 1 cut(s) | 702 |
| ErhI | CCWWGG | 2 cut(s) | 777, 1458 |
| FalI | AAGNNNNNCTT | 4 cut(s) | 444, 476, 1058, 1090 |
| FaqI | GGGAC | 2 cut(s) | 137, 226 |
| FbaI | TGATCA | 1 cut(s) | 661 |
| FblI | GTMKAC | 1 cut(s) | 295 |
| Fnu4HI | GCNGC | 7 cut(s) | 130, 209, 252, 645, 683, 897, 1252 |
| FokI | GGATG | 5 cut(s) | 35, 571, 794, 942, 1071 |
| FriOI | GRGCYC | 1 cut(s) | 702 |
| Fsp4HI | GCNGC | 7 cut(s) | 130, 209, 252, 645, 683, 897, 1252 |
| FspBI | CTAG | 4 cut(s) | 176, 212, 989, 1452 |
| GlaI | GCGC | 4 cut(s) | 374, 681, 1166, 1318 |
| GluI | GCNGC | 7 cut(s) | 130, 209, 252, 645, 683, 897, 1252 |
| GsaI | CCCAGC | 1 cut(s) | 821 |
| HaeIII | GGCC | 6 cut(s) | 216, 254, 437, 822, 875, 899 |
| HhaI | GCGC | 4 cut(s) | 375, 682, 1167, 1319 |
| Hin6I | GCGC | 4 cut(s) | 373, 680, 1165, 1317 |
| HinP1I | GCGC | 4 cut(s) | 373, 680, 1165, 1317 |
| HincII | GTYRAC | 1 cut(s) | 544 |
| HindII | GTYRAC | 1 cut(s) | 544 |
| HinfI | GANTC | 4 cut(s) | 466, 839, 852, 1025 |
| HphI | GGTGA | 2 cut(s) | 628, 694 |
| Hpy166II | GTNNAC | 4 cut(s) | 46, 296, 544, 907 |
| Hpy188I | TCNGA | 9 cut(s) | 108, 465, 558, 835, 844, 943, 1030, 1155, 1494 |
| Hpy188III | TCNNGA | 4 cut(s) | 305, 479, 1220, 1394 |
| Hpy8I | GTNNAC | 4 cut(s) | 46, 296, 544, 907 |
| Hpy99I | CGWCG | 2 cut(s) | 501, 764 |
| HpyAV | CCTTC | 5 cut(s) | 142, 302, 1060, 1488, 1499 |
| HpyCH4III | ACNGT | 4 cut(s) | 1135, 1293, 1342, 1350 |
| HpyCH4IV | ACGT | 2 cut(s) | 228, 1312 |
| HpyF10VI | GCNNNNNNNGC | 5 cut(s) | 379, 691, 737, 1282, 1333 |
| HpyF3I | CTNAG | 7 cut(s) | 354, 407, 515, 901, 940, 950, 1428 |
| HpySE526I | ACGT | 2 cut(s) | 228, 1312 |
| HspAI | GCGC | 4 cut(s) | 373, 680, 1165, 1317 |
| KpnI | GGTACC | 1 cut(s) | 1097 |
| Ksp22I | TGATCA | 1 cut(s) | 661 |
| Kzo9I | GATC | 5 cut(s) | 163, 420, 525, 661, 1216 |
| LmnI | GCTCC | 3 cut(s) | 985, 1110, 1473 |
| Lsp1109I | GCAGC | 3 cut(s) | 141, 656, 1263 |
| LweI | GCATC | 3 cut(s) | 81, 727, 964 |
| MaeI | CTAG | 4 cut(s) | 176, 212, 989, 1452 |
| MaeII | ACGT | 2 cut(s) | 228, 1312 |
| MalI | GATC | 5 cut(s) | 165, 422, 527, 663, 1218 |
| MbiI | CCGCTC | 1 cut(s) | 251 |
| MboI | GATC | 5 cut(s) | 163, 420, 525, 661, 1216 |
| MfeI | CAATTG | 2 cut(s) | 24, 1188 |
| MflI | RGATCY | 3 cut(s) | 163, 525, 1216 |
| MhlI | GDGCHC | 1 cut(s) | 702 |
| MluCI | AATT | 7 cut(s) | 5, 24, 401, 635, 1040, 1159, 1188 |
| MluI | ACGCGT | 1 cut(s) | 859 |
| MmeI | TCCRAC | 3 cut(s) | 536, 864, 1398 |
| MseI | TTAA | 1 cut(s) | 1266 |
| MslI | CAYNNNNRTG | 4 cut(s) | 203, 309, 614, 1061 |
| MspR9I | CCNGG | 1 cut(s) | 102 |
| MunI | CAATTG | 2 cut(s) | 24, 1188 |
| Mva1269I | GAATGC | 1 cut(s) | 1329 |
| MvaI | CCWGG | 1 cut(s) | 102 |
| MvnI | CGCG | 4 cut(s) | 375, 861, 896, 1165 |
| MwoI | GCNNNNNNNGC | 5 cut(s) | 379, 691, 737, 1282, 1333 |
| NdeII | GATC | 5 cut(s) | 163, 420, 525, 661, 1216 |
| NlaIV | GGNNCC | 3 cut(s) | 153, 504, 1095 |
| NmeAIII | GCCGAG | 2 cut(s) | 280, 516 |
| NspI | RCATGY | 5 cut(s) | 208, 397, 869, 892, 1405 |
| OliI | CACNNNNGTG | 1 cut(s) | 614 |
| PaeI | GCATGC | 1 cut(s) | 397 |
| PagI | TCATGA | 1 cut(s) | 304 |
| PceI | AGGCCT | 1 cut(s) | 216 |
| PciI | ACATGT | 2 cut(s) | 888, 1401 |
| PctI | GAATGC | 1 cut(s) | 1329 |
| PfeI | GAWTC | 4 cut(s) | 466, 839, 852, 1025 |
| PkrI | GCNGC | 7 cut(s) | 131, 210, 253, 646, 684, 898, 1253 |
| PpuMI | RGGWCCY | 1 cut(s) | 151 |
| PscI | ACATGT | 2 cut(s) | 888, 1401 |
| Psp1406I | AACGTT | 1 cut(s) | 228 |
| Psp5II | RGGWCCY | 1 cut(s) | 151 |
| Psp6I | CCWGG | 1 cut(s) | 100 |
| PspFI | CCCAGC | 1 cut(s) | 817 |
| PspGI | CCWGG | 1 cut(s) | 100 |
| PspN4I | GGNNCC | 3 cut(s) | 153, 504, 1095 |
| PspPI | GGNCC | 5 cut(s) | 104, 151, 820, 874, 1455 |
| PspPPI | RGGWCCY | 1 cut(s) | 151 |
| PsuI | RGATCY | 3 cut(s) | 163, 525, 1216 |
| RsaI | GTAC | 6 cut(s) | 203, 578, 1049, 1095, 1233, 1290 |
| RsaNI | GTAC | 6 cut(s) | 202, 577, 1048, 1094, 1232, 1289 |
| RseI | CAYNNNNRTG | 4 cut(s) | 203, 309, 614, 1061 |
| SaqAI | TTAA | 1 cut(s) | 1266 |
| SatI | GCNGC | 7 cut(s) | 130, 209, 252, 645, 683, 897, 1252 |
| Sau3AI | GATC | 5 cut(s) | 163, 420, 525, 661, 1216 |
| Sau96I | GGNCC | 5 cut(s) | 104, 151, 820, 874, 1455 |
| ScaI | AGTACT | 1 cut(s) | 1049 |
| ScrFI | CCNGG | 1 cut(s) | 102 |
| SduI | GDGCHC | 1 cut(s) | 702 |
| SfaNI | GCATC | 3 cut(s) | 81, 727, 964 |
| SinI | GGWCC | 3 cut(s) | 104, 151, 1455 |
| SmiMI | CAYNNNNRTG | 4 cut(s) | 203, 309, 614, 1061 |
| SmlI | CTYRAG | 1 cut(s) | 1075 |
| SmoI | CTYRAG | 1 cut(s) | 1075 |
| SphI | GCATGC | 1 cut(s) | 397 |
| Sse9I | AATT | 7 cut(s) | 5, 24, 401, 635, 1040, 1159, 1188 |
| SseBI | AGGCCT | 1 cut(s) | 216 |
| SsiI | CCGC | 5 cut(s) | 208, 251, 614, 683, 896 |
| SspI | AATATT | 1 cut(s) | 1258 |
| SspMI | CTAG | 4 cut(s) | 176, 212, 989, 1452 |
| StuI | AGGCCT | 1 cut(s) | 216 |
| StyD4I | CCNGG | 1 cut(s) | 100 |
| StyI | CCWWGG | 2 cut(s) | 777, 1458 |
| TaaI | ACNGT | 4 cut(s) | 1135, 1293, 1342, 1350 |
| TaiI | ACGT | 2 cut(s) | 231, 1315 |
| TaqI | TCGA | 6 cut(s) | 399, 496, 759, 855, 1122, 1137 |
| TasI | AATT | 7 cut(s) | 5, 24, 401, 635, 1040, 1159, 1188 |
| TatI | WGTACW | 3 cut(s) | 201, 576, 1047 |
| TauI | GCSGC | 4 cut(s) | 211, 254, 685, 899 |
| TfiI | GAWTC | 4 cut(s) | 466, 839, 852, 1025 |
| Tru1I | TTAA | 1 cut(s) | 1266 |
| Tru9I | TTAA | 1 cut(s) | 1266 |
| TscAI | CASTG | 3 cut(s) | 523, 909, 1138 |
| TseI | GCWGC | 3 cut(s) | 129, 644, 1251 |
| TspDTI | ATGAA | 6 cut(s) | 321, 635, 728, 798, 983, 1470 |
| TspGWI | ACGGA | 1 cut(s) | 463 |
| TspRI | CASTG | 3 cut(s) | 523, 909, 1138 |
| VpaK11BI | GGWCC | 3 cut(s) | 104, 151, 1455 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 306 |
| XapI | RAATTY | 2 cut(s) | 5, 1159 |
| XceI | RCATGY | 5 cut(s) | 208, 397, 869, 892, 1405 |
| XmiI | GTMKAC | 1 cut(s) | 295 |
| XspI | CTAG | 4 cut(s) | 176, 212, 989, 1452 |
| ZrmI | AGTACT | 1 cut(s) | 1049 |