Cytochrome p450
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccB7I | CCANNNNNTGG | 1 cut(s) | 454 |
| AciI | CCGC | 2 cut(s) | 196, 287 |
| AclWI | GGATC | 1 cut(s) | 780 |
| AcoI | YGGCCR | 1 cut(s) | 319 |
| AcsI | RAATTY | 3 cut(s) | 581, 1260, 1364 |
| AcuI | CTGAAG | 2 cut(s) | 1080, 1173 |
| AdeI | CACNNNGTG | 1 cut(s) | 454 |
| AfaI | GTAC | 2 cut(s) | 191, 986 |
| AfiI | CCNNNNNNNGG | 6 cut(s) | 63, 113, 296, 454, 646, 647 |
| AflIII | ACRYGT | 3 cut(s) | 366, 490, 1332 |
| AgsI | TTSAA | 4 cut(s) | 421, 614, 764, 1265 |
| AjiI | CACGTC | 1 cut(s) | 538 |
| AjnI | CCWGG | 3 cut(s) | 56, 88, 1255 |
| AloI | GAACNNNNNNTCC | 2 cut(s) | 1362, 1394 |
| AluBI | AGCT | 4 cut(s) | 247, 510, 635, 1222 |
| AluI | AGCT | 4 cut(s) | 247, 510, 635, 1222 |
| Alw21I | GWGCWC | 1 cut(s) | 383 |
| Alw26I | GTCTC | 4 cut(s) | 229, 528, 880, 1128 |
| Alw44I | GTGCAC | 1 cut(s) | 379 |
| AlwI | GGATC | 1 cut(s) | 780 |
| Ama87I | CYCGRG | 1 cut(s) | 234 |
| AoxI | GGCC | 5 cut(s) | 165, 202, 284, 319, 1386 |
| ApaLI | GTGCAC | 1 cut(s) | 379 |
| ApeKI | GCWGC | 4 cut(s) | 239, 476, 635, 1251 |
| ApoI | RAATTY | 3 cut(s) | 581, 1260, 1364 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 257, 289 |
| AspA2I | CCTAGG | 1 cut(s) | 1382 |
| AspLEI | GCGC | 3 cut(s) | 37, 533, 1251 |
| AspS9I | GGNCC | 3 cut(s) | 92, 166, 1386 |
| AsuHPI | GGTGA | 3 cut(s) | 191, 913, 1329 |
| AvaI | CYCGRG | 1 cut(s) | 234 |
| AvaII | GGWCC | 1 cut(s) | 92 |
| AvrII | CCTAGG | 1 cut(s) | 1382 |
| BaeGI | GKGCMC | 1 cut(s) | 383 |
| BalI | TGGCCA | 1 cut(s) | 321 |
| BarI | GAAGNNNNNNTAC | 2 cut(s) | 968, 1000 |
| Bbv12I | GWGCWC | 1 cut(s) | 383 |
| BbvI | GCAGC | 4 cut(s) | 251, 488, 622, 1238 |
| BccI | CCATC | 4 cut(s) | 544, 946, 1056, 1417 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 1233, 1267 |
| BciT130I | CCWGG | 3 cut(s) | 58, 90, 1257 |
| BcoDI | GTCTC | 4 cut(s) | 229, 528, 880, 1128 |
| BfaI | CTAG | 3 cut(s) | 768, 1268, 1383 |
| BfmI | CTRYAG | 1 cut(s) | 474 |
| BfoI | RGCGCY | 1 cut(s) | 1252 |
| BglII | AGATCT | 2 cut(s) | 151, 1198 |
| BisI | GCNGC | 5 cut(s) | 240, 287, 477, 636, 1252 |
| BlnI | CCTAGG | 1 cut(s) | 1382 |
| BlsI | GCNGC | 5 cut(s) | 241, 288, 478, 637, 1253 |
| BmcAI | AGTACT | 1 cut(s) | 986 |
| Bme1390I | CCNGG | 3 cut(s) | 58, 90, 1257 |
| Bme18I | GGWCC | 1 cut(s) | 92 |
| BmeT110I | CYCGRG | 1 cut(s) | 234 |
| BmgBI | CACGTC | 1 cut(s) | 538 |
| BmgT120I | GGNCC | 3 cut(s) | 92, 166, 1386 |
| BmiI | GGNNCC | 1 cut(s) | 1380 |
| BmrFI | CCNGG | 3 cut(s) | 58, 90, 1257 |
| BmrI | ACTGGG | 1 cut(s) | 1324 |
| BmsI | GCATC | 2 cut(s) | 166, 766 |
| BmuI | ACTGGG | 1 cut(s) | 1324 |
| BplI | GAGNNNNNCTC | 4 cut(s) | 70, 102, 335, 367 |
| BpuEI | CTTGAG | 3 cut(s) | 352, 407, 1033 |
| BsaBI | GATNNNNATC | 1 cut(s) | 156 |
| BsaI | GGTCTC | 1 cut(s) | 1128 |
| BsaJI | CCNNGG | 8 cut(s) | 56, 57, 480, 513, 685, 792, 1255, 1382 |
| BsaWI | WCCGGW | 2 cut(s) | 1024, 1278 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 666, 696 |
| Bsc4I | CCNNNNNNNGG | 6 cut(s) | 63, 113, 296, 454, 646, 647 |
| Bse1I | ACTGG | 4 cut(s) | 168, 362, 1319, 1404 |
| Bse3DI | GCAATG | 1 cut(s) | 666 |
| Bse8I | GATNNNNATC | 1 cut(s) | 156 |
| BseBI | CCWGG | 3 cut(s) | 58, 90, 1257 |
| BseDI | CCNNGG | 8 cut(s) | 56, 57, 480, 513, 685, 792, 1255, 1382 |
| BseGI | GGATG | 4 cut(s) | 555, 580, 957, 1021 |
| BseJI | GATNNNNATC | 1 cut(s) | 156 |
| BseLI | CCNNNNNNNGG | 6 cut(s) | 63, 113, 296, 454, 646, 647 |
| BseMI | GCAATG | 1 cut(s) | 666 |
| BseMII | CTCAG | 2 cut(s) | 333, 1164 |
| BseNI | ACTGG | 4 cut(s) | 168, 362, 1319, 1404 |
| BseRI | GAGGAG | 2 cut(s) | 767, 974 |
| BseSI | GKGCMC | 1 cut(s) | 383 |
| BseXI | GCAGC | 4 cut(s) | 251, 488, 622, 1238 |
| BshFI | GGCC | 5 cut(s) | 167, 204, 286, 321, 1388 |
| BsiHKAI | GWGCWC | 1 cut(s) | 383 |
| BsiHKCI | CYCGRG | 1 cut(s) | 234 |
| BsiSI | CCGG | 2 cut(s) | 1025, 1279 |
| BslFI | GGGAC | 1 cut(s) | 839 |
| BslI | CCNNNNNNNGG | 6 cut(s) | 63, 113, 296, 454, 646, 647 |
| BsmAI | GTCTC | 4 cut(s) | 229, 528, 880, 1128 |
| BsmBI | CGTCTC | 1 cut(s) | 229 |
| BsmFI | GGGAC | 1 cut(s) | 839 |
| BsmI | GAATGC | 1 cut(s) | 892 |
| BsnI | GGCC | 5 cut(s) | 167, 204, 286, 321, 1388 |
| Bso31I | GGTCTC | 1 cut(s) | 1128 |
| BsoBI | CYCGRG | 1 cut(s) | 234 |
| Bsp1286I | GDGCHC | 1 cut(s) | 383 |
| Bsp1407I | TGTACA | 1 cut(s) | 189 |
| Bsp143I | GATC | 5 cut(s) | 151, 502, 679, 772, 1198 |
| BspACI | CCGC | 2 cut(s) | 196, 287 |
| BspANI | GGCC | 5 cut(s) | 167, 204, 286, 321, 1388 |
| BspCNI | CTCAG | 2 cut(s) | 334, 1165 |
| BspHI | TCATGA | 1 cut(s) | 292 |
| BspLI | GGNNCC | 1 cut(s) | 1380 |
| BspMAI | CTGCAG | 1 cut(s) | 478 |
| BspPI | GGATC | 1 cut(s) | 780 |
| BspTNI | GGTCTC | 1 cut(s) | 1128 |
| BsrDI | GCAATG | 1 cut(s) | 666 |
| BsrGI | TGTACA | 1 cut(s) | 189 |
| BsrI | ACTGG | 4 cut(s) | 168, 362, 1319, 1404 |
| BssECI | CCNNGG | 8 cut(s) | 56, 57, 480, 513, 685, 792, 1255, 1382 |
| BssMI | GATC | 5 cut(s) | 151, 502, 679, 772, 1198 |
| BssT1I | CCWWGG | 4 cut(s) | 480, 513, 792, 1382 |
| Bst2UI | CCWGG | 3 cut(s) | 58, 90, 1257 |
| Bst4CI | ACNGT | 2 cut(s) | 1072, 1378 |
| Bst6I | CTCTTC | 3 cut(s) | 71, 232, 432 |
| BstAPI | GCANNNNNTGC | 2 cut(s) | 378, 1300 |
| BstAUI | TGTACA | 1 cut(s) | 189 |
| BstC8I | GCNNGC | 5 cut(s) | 33, 280, 284, 623, 834 |
| BstDEI | CTNAG | 5 cut(s) | 342, 395, 506, 527, 1173 |
| BstDSI | CCRYGG | 1 cut(s) | 685 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 294 |
| BstF5I | GGATG | 4 cut(s) | 555, 580, 957, 1021 |
| BstH2I | RGCGCY | 1 cut(s) | 1252 |
| BstHHI | GCGC | 3 cut(s) | 37, 533, 1251 |
| BstKTI | GATC | 5 cut(s) | 154, 505, 682, 775, 1201 |
| BstMAI | GTCTC | 4 cut(s) | 229, 528, 880, 1128 |
| BstMBI | GATC | 5 cut(s) | 151, 502, 679, 772, 1198 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 378, 1300 |
| BstNI | CCWGG | 3 cut(s) | 58, 90, 1257 |
| BstNSI | RCATGY | 4 cut(s) | 196, 370, 832, 1336 |
| BstSCI | CCNGG | 3 cut(s) | 56, 88, 1255 |
| BstSFI | CTRYAG | 1 cut(s) | 474 |
| BstSLI | GKGCMC | 1 cut(s) | 383 |
| BstV1I | GCAGC | 4 cut(s) | 251, 488, 622, 1238 |
| BstX2I | RGATCY | 2 cut(s) | 151, 1198 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 1146 |
| BstYI | RGATCY | 2 cut(s) | 151, 1198 |
| BsuRI | GGCC | 5 cut(s) | 167, 204, 286, 321, 1388 |
| BtgI | CCRYGG | 1 cut(s) | 685 |
| BtrI | CACGTC | 1 cut(s) | 538 |
| BtsCI | GGATG | 4 cut(s) | 555, 580, 957, 1021 |
| BtsIMutI | CAGTG | 1 cut(s) | 1068 |
| Cac8I | GCNNGC | 5 cut(s) | 33, 280, 284, 623, 834 |
| CciI | TCATGA | 1 cut(s) | 292 |
| CfoI | GCGC | 3 cut(s) | 37, 533, 1251 |
| Cfr13I | GGNCC | 3 cut(s) | 92, 166, 1386 |
| Csp6I | GTAC | 2 cut(s) | 190, 985 |
| CviQI | GTAC | 2 cut(s) | 190, 985 |
| DdeI | CTNAG | 5 cut(s) | 342, 395, 506, 527, 1173 |
| DpnI | GATC | 5 cut(s) | 153, 504, 681, 774, 1200 |
| DpnII | GATC | 5 cut(s) | 151, 502, 679, 772, 1198 |
| DraI | TTTAAA | 1 cut(s) | 601 |
| DraIII | CACNNNGTG | 1 cut(s) | 454 |
| EaeI | YGGCCR | 1 cut(s) | 319 |
| Eam1104I | CTCTTC | 3 cut(s) | 71, 232, 432 |
| EarI | CTCTTC | 3 cut(s) | 71, 232, 432 |
| Eco130I | CCWWGG | 4 cut(s) | 480, 513, 792, 1382 |
| Eco147I | AGGCCT | 1 cut(s) | 204 |
| Eco31I | GGTCTC | 1 cut(s) | 1128 |
| Eco47I | GGWCC | 1 cut(s) | 92 |
| Eco57I | CTGAAG | 2 cut(s) | 1080, 1173 |
| Eco88I | CYCGRG | 1 cut(s) | 234 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 294 |
| EcoRI | GAATTC | 1 cut(s) | 1260 |
| EcoRII | CCWGG | 3 cut(s) | 56, 88, 1255 |
| EcoT14I | CCWWGG | 4 cut(s) | 480, 513, 792, 1382 |
| ErhI | CCWWGG | 4 cut(s) | 480, 513, 792, 1382 |
| Esp3I | CGTCTC | 1 cut(s) | 229 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 995, 1027 |
| FaqI | GGGAC | 1 cut(s) | 839 |
| Fnu4HI | GCNGC | 5 cut(s) | 240, 287, 477, 636, 1252 |
| FokI | GGATG | 4 cut(s) | 562, 587, 964, 1008 |
| Fsp4HI | GCNGC | 5 cut(s) | 240, 287, 477, 636, 1252 |
| FspBI | CTAG | 3 cut(s) | 768, 1268, 1383 |
| GlaI | GCGC | 3 cut(s) | 36, 532, 1250 |
| GluI | GCNGC | 5 cut(s) | 240, 287, 477, 636, 1252 |
| HaeII | RGCGCY | 1 cut(s) | 1252 |
| HaeIII | GGCC | 5 cut(s) | 167, 204, 286, 321, 1388 |
| HapII | CCGG | 2 cut(s) | 1025, 1279 |
| HhaI | GCGC | 3 cut(s) | 37, 533, 1251 |
| Hin6I | GCGC | 3 cut(s) | 35, 531, 1249 |
| HinP1I | GCGC | 3 cut(s) | 35, 531, 1249 |
| HinfI | GANTC | 5 cut(s) | 458, 470, 610, 690, 962 |
| HpaII | CCGG | 2 cut(s) | 1025, 1279 |
| HphI | GGTGA | 3 cut(s) | 191, 913, 1329 |
| Hpy166II | GTNNAC | 2 cut(s) | 381, 715 |
| Hpy188I | TCNGA | 5 cut(s) | 96, 880, 898, 941, 967 |
| Hpy188III | TCNNGA | 6 cut(s) | 293, 386, 467, 614, 1239, 1325 |
| Hpy8I | GTNNAC | 2 cut(s) | 381, 715 |
| HpyAV | CCTTC | 7 cut(s) | 290, 427, 611, 700, 997, 1239, 1430 |
| HpyCH4III | ACNGT | 2 cut(s) | 1072, 1378 |
| HpyCH4IV | ACGT | 2 cut(s) | 490, 537 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 378, 1300 |
| HpyF3I | CTNAG | 5 cut(s) | 342, 395, 506, 527, 1173 |
| HpySE526I | ACGT | 2 cut(s) | 490, 537 |
| HspAI | GCGC | 3 cut(s) | 35, 531, 1249 |
| Kzo9I | GATC | 5 cut(s) | 151, 502, 679, 772, 1198 |
| LmnI | GCTCC | 4 cut(s) | 515, 632, 676, 754 |
| Lsp1109I | GCAGC | 4 cut(s) | 251, 488, 622, 1238 |
| LweI | GCATC | 2 cut(s) | 166, 766 |
| MaeI | CTAG | 3 cut(s) | 768, 1268, 1383 |
| MaeII | ACGT | 2 cut(s) | 490, 537 |
| MaeIII | GTNAC | 6 cut(s) | 138, 179, 362, 390, 538, 1335 |
| MalI | GATC | 5 cut(s) | 153, 504, 681, 774, 1200 |
| MboI | GATC | 5 cut(s) | 151, 502, 679, 772, 1198 |
| MfeI | CAATTG | 2 cut(s) | 861, 1125 |
| MflI | RGATCY | 2 cut(s) | 151, 1198 |
| MhlI | GDGCHC | 1 cut(s) | 383 |
| MlsI | TGGCCA | 1 cut(s) | 321 |
| MluCI | AATT | 7 cut(s) | 581, 626, 861, 1125, 1260, 1364, 1404 |
| MluNI | TGGCCA | 1 cut(s) | 321 |
| MlyI | GAGTC | 2 cut(s) | 479, 971 |
| MmeI | TCCRAC | 2 cut(s) | 919, 960 |
| Mox20I | TGGCCA | 1 cut(s) | 321 |
| MscI | TGGCCA | 1 cut(s) | 321 |
| MseI | TTAA | 3 cut(s) | 600, 1074, 1311 |
| MslI | CAYNNNNRTG | 3 cut(s) | 191, 297, 998 |
| Msp20I | TGGCCA | 1 cut(s) | 321 |
| MspI | CCGG | 2 cut(s) | 1025, 1279 |
| MspR9I | CCNGG | 3 cut(s) | 58, 90, 1257 |
| MunI | CAATTG | 2 cut(s) | 861, 1125 |
| Mva1269I | GAATGC | 1 cut(s) | 892 |
| MvaI | CCWGG | 3 cut(s) | 58, 90, 1257 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 378, 1300 |
| NdeII | GATC | 5 cut(s) | 151, 502, 679, 772, 1198 |
| NlaIV | GGNNCC | 1 cut(s) | 1380 |
| NmeAIII | GCCGAG | 1 cut(s) | 268 |
| NmuCI | GTSAC | 4 cut(s) | 179, 362, 538, 1335 |
| NspI | RCATGY | 4 cut(s) | 196, 370, 832, 1336 |
| PaeR7I | CTCGAG | 1 cut(s) | 234 |
| PagI | TCATGA | 1 cut(s) | 292 |
| PasI | CCCWGGG | 1 cut(s) | 57 |
| PceI | AGGCCT | 1 cut(s) | 204 |
| PciI | ACATGT | 2 cut(s) | 366, 1332 |
| PctI | GAATGC | 1 cut(s) | 892 |
| PfeI | GAWTC | 3 cut(s) | 458, 610, 690 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 454 |
| PkrI | GCNGC | 5 cut(s) | 241, 288, 478, 637, 1253 |
| PleI | GAGTC | 2 cut(s) | 478, 970 |
| PpsI | GAGTC | 2 cut(s) | 478, 970 |
| PscI | ACATGT | 2 cut(s) | 366, 1332 |
| Psp6I | CCWGG | 3 cut(s) | 56, 88, 1255 |
| PspGI | CCWGG | 3 cut(s) | 56, 88, 1255 |
| PspN4I | GGNNCC | 1 cut(s) | 1380 |
| PspPI | GGNCC | 3 cut(s) | 92, 166, 1386 |
| PspXI | VCTCGAGB | 1 cut(s) | 234 |
| PstI | CTGCAG | 1 cut(s) | 478 |
| PsuI | RGATCY | 2 cut(s) | 151, 1198 |
| RsaI | GTAC | 2 cut(s) | 191, 986 |
| RsaNI | GTAC | 2 cut(s) | 190, 985 |
| RseI | CAYNNNNRTG | 3 cut(s) | 191, 297, 998 |
| SaqAI | TTAA | 3 cut(s) | 600, 1074, 1311 |
| SatI | GCNGC | 5 cut(s) | 240, 287, 477, 636, 1252 |
| Sau3AI | GATC | 5 cut(s) | 151, 502, 679, 772, 1198 |
| Sau96I | GGNCC | 3 cut(s) | 92, 166, 1386 |
| ScaI | AGTACT | 1 cut(s) | 986 |
| SchI | GAGTC | 2 cut(s) | 479, 971 |
| ScrFI | CCNGG | 3 cut(s) | 58, 90, 1257 |
| SduI | GDGCHC | 1 cut(s) | 383 |
| SfaNI | GCATC | 2 cut(s) | 166, 766 |
| SfcI | CTRYAG | 1 cut(s) | 474 |
| Sfr274I | CTCGAG | 1 cut(s) | 234 |
| SinI | GGWCC | 1 cut(s) | 92 |
| SlaI | CTCGAG | 1 cut(s) | 234 |
| SmiMI | CAYNNNNRTG | 3 cut(s) | 191, 297, 998 |
| SmlI | CTYRAG | 4 cut(s) | 234, 331, 386, 1012 |
| SmoI | CTYRAG | 4 cut(s) | 234, 331, 386, 1012 |
| Sse9I | AATT | 7 cut(s) | 581, 626, 861, 1125, 1260, 1364, 1404 |
| SseBI | AGGCCT | 1 cut(s) | 204 |
| SsiI | CCGC | 2 cut(s) | 196, 287 |
| SspMI | CTAG | 3 cut(s) | 768, 1268, 1383 |
| StuI | AGGCCT | 1 cut(s) | 204 |
| StyD4I | CCNGG | 3 cut(s) | 56, 88, 1255 |
| StyI | CCWWGG | 4 cut(s) | 480, 513, 792, 1382 |
| TaaI | ACNGT | 2 cut(s) | 1072, 1378 |
| TaiI | ACGT | 2 cut(s) | 493, 540 |
| TaqI | TCGA | 3 cut(s) | 235, 466, 678 |
| TasI | AATT | 7 cut(s) | 581, 626, 861, 1125, 1260, 1364, 1404 |
| TatI | WGTACW | 2 cut(s) | 189, 984 |
| TauI | GCSGC | 1 cut(s) | 289 |
| TfiI | GAWTC | 3 cut(s) | 458, 610, 690 |
| Tru1I | TTAA | 3 cut(s) | 600, 1074, 1311 |
| Tru9I | TTAA | 3 cut(s) | 600, 1074, 1311 |
| TscAI | CASTG | 1 cut(s) | 1075 |
| TseFI | GTSAC | 4 cut(s) | 179, 362, 538, 1335 |
| TseI | GCWGC | 4 cut(s) | 239, 476, 635, 1251 |
| Tsp45I | GTSAC | 4 cut(s) | 179, 362, 538, 1335 |
| TspDTI | ATGAA | 5 cut(s) | 309, 719, 809, 923, 1156 |
| TspGWI | ACGGA | 2 cut(s) | 674, 1373 |
| TspRI | CASTG | 1 cut(s) | 1075 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 454 |
| VneI | GTGCAC | 1 cut(s) | 379 |
| VpaK11BI | GGWCC | 1 cut(s) | 92 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 294 |
| XapI | RAATTY | 3 cut(s) | 581, 1260, 1364 |
| XceI | RCATGY | 4 cut(s) | 196, 370, 832, 1336 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 1396 |
| XhoI | CTCGAG | 1 cut(s) | 234 |
| XmaJI | CCTAGG | 1 cut(s) | 1382 |
| XspI | CTAG | 3 cut(s) | 768, 1268, 1383 |
| ZrmI | AGTACT | 1 cut(s) | 986 |