Cytochrome p450
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc36I | ACCTGC | 1 cut(s) | 138 |
| AccB1I | GGYRCC | 1 cut(s) | 1087 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 1348 |
| AccII | CGCG | 1 cut(s) | 955 |
| AciI | CCGC | 2 cut(s) | 953, 955 |
| AclWI | GGATC | 2 cut(s) | 168, 607 |
| AcoI | YGGCCR | 1 cut(s) | 1297 |
| AcsI | RAATTY | 2 cut(s) | 337, 584 |
| AcuI | CTGAAG | 1 cut(s) | 1469 |
| AfaI | GTAC | 4 cut(s) | 48, 572, 842, 1043 |
| AfiI | CCNNNNNNNGG | 4 cut(s) | 122, 157, 650, 1348 |
| AflIII | ACRYGT | 1 cut(s) | 135 |
| AgsI | TTSAA | 6 cut(s) | 305, 430, 476, 604, 767, 1277 |
| AjiI | CACGTC | 1 cut(s) | 1268 |
| AjnI | CCWGG | 2 cut(s) | 97, 687 |
| AluBI | AGCT | 7 cut(s) | 256, 308, 451, 546, 869, 1345, 1384 |
| AluI | AGCT | 7 cut(s) | 256, 308, 451, 546, 869, 1345, 1384 |
| Alw21I | GWGCWC | 1 cut(s) | 392 |
| Alw26I | GTCTC | 3 cut(s) | 531, 1058, 1185 |
| Alw44I | GTGCAC | 1 cut(s) | 388 |
| AlwI | GGATC | 2 cut(s) | 168, 607 |
| AoxI | GGCC | 3 cut(s) | 211, 293, 1297 |
| ApaLI | GTGCAC | 1 cut(s) | 388 |
| ApeKI | GCWGC | 9 cut(s) | 248, 308, 408, 482, 546, 635, 1096, 1109, 1489 |
| ApoI | RAATTY | 2 cut(s) | 337, 584 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 266, 298 |
| Asp700I | GAANNNNTTC | 2 cut(s) | 762, 1136 |
| AspA2I | CCTAGG | 1 cut(s) | 1445 |
| AspLEI | GCGC | 3 cut(s) | 207, 705, 896 |
| AspS9I | GGNCC | 2 cut(s) | 101, 1339 |
| AsuC2I | CCSGG | 2 cut(s) | 158, 1314 |
| AsuHPI | GGTGA | 3 cut(s) | 146, 665, 970 |
| AvaII | GGWCC | 2 cut(s) | 101, 1339 |
| AvrII | CCTAGG | 1 cut(s) | 1445 |
| AxyI | CCTNAGG | 1 cut(s) | 243 |
| BaeGI | GKGCMC | 1 cut(s) | 392 |
| BanI | GGYRCC | 1 cut(s) | 1087 |
| BarI | GAAGNNNNNNTAC | 2 cut(s) | 1025, 1057 |
| BauI | CACGAG | 1 cut(s) | 1158 |
| Bbv12I | GWGCWC | 1 cut(s) | 392 |
| BbvI | GCAGC | 8 cut(s) | 235, 295, 420, 469, 533, 647, 1083, 1121 |
| BccI | CCATC | 2 cut(s) | 547, 1411 |
| BceAI | ACGGC | 1 cut(s) | 202 |
| BciT130I | CCWGG | 2 cut(s) | 99, 689 |
| BcnI | CCSGG | 2 cut(s) | 158, 1314 |
| BcoDI | GTCTC | 3 cut(s) | 531, 1058, 1185 |
| BfaI | CTAG | 3 cut(s) | 173, 1197, 1446 |
| BfmI | CTRYAG | 1 cut(s) | 771 |
| BfuAI | ACCTGC | 1 cut(s) | 138 |
| BisI | GCNGC | 9 cut(s) | 249, 309, 409, 483, 547, 636, 1097, 1110, 1490 |
| BlnI | CCTAGG | 1 cut(s) | 1445 |
| BlpI | GCTNAGC | 3 cut(s) | 252, 404, 1452 |
| BlsI | GCNGC | 9 cut(s) | 250, 310, 410, 484, 548, 637, 1098, 1111, 1491 |
| BmcAI | AGTACT | 1 cut(s) | 1043 |
| Bme1390I | CCNGG | 4 cut(s) | 99, 158, 689, 1314 |
| Bme18I | GGWCC | 2 cut(s) | 101, 1339 |
| BmgBI | CACGTC | 1 cut(s) | 1268 |
| BmgT120I | GGNCC | 2 cut(s) | 101, 1339 |
| BmiI | GGNNCC | 3 cut(s) | 103, 361, 1089 |
| BmrFI | CCNGG | 4 cut(s) | 99, 158, 689, 1314 |
| BmsI | GCATC | 5 cut(s) | 175, 721, 958, 1201, 1465 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 79, 111 |
| BpmI | CTGGAG | 1 cut(s) | 671 |
| Bpu10I | CCTNAGC | 1 cut(s) | 208 |
| Bpu1102I | GCTNAGC | 3 cut(s) | 252, 404, 1452 |
| BpuEI | CTTGAG | 1 cut(s) | 416 |
| BpuMI | CCSGG | 2 cut(s) | 158, 1314 |
| Bsa29I | ATCGAT | 1 cut(s) | 1254 |
| BsaBI | GATNNNNATC | 1 cut(s) | 165 |
| BsaI | GGTCTC | 2 cut(s) | 1058, 1185 |
| BsaJI | CCNNGG | 4 cut(s) | 688, 953, 1312, 1445 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 669, 699 |
| Bsc4I | CCNNNNNNNGG | 4 cut(s) | 122, 157, 650, 1348 |
| Bse118I | RCCGGY | 1 cut(s) | 1299 |
| Bse1I | ACTGG | 2 cut(s) | 486, 1082 |
| Bse21I | CCTNAGG | 1 cut(s) | 243 |
| Bse3DI | GCAATG | 3 cut(s) | 903, 1091, 1132 |
| Bse8I | GATNNNNATC | 1 cut(s) | 165 |
| BseBI | CCWGG | 2 cut(s) | 99, 689 |
| BseCI | ATCGAT | 1 cut(s) | 1254 |
| BseDI | CCNNGG | 4 cut(s) | 688, 953, 1312, 1445 |
| BseGI | GGATG | 3 cut(s) | 236, 949, 1078 |
| BseJI | GATNNNNATC | 1 cut(s) | 165 |
| BseLI | CCNNNNNNNGG | 4 cut(s) | 122, 157, 650, 1348 |
| BseMI | GCAATG | 3 cut(s) | 903, 1091, 1132 |
| BseMII | CTCAG | 5 cut(s) | 243, 257, 418, 523, 1398 |
| BseNI | ACTGG | 2 cut(s) | 486, 1082 |
| BseRI | GAGGAG | 1 cut(s) | 1439 |
| BseSI | GKGCMC | 1 cut(s) | 392 |
| BseXI | GCAGC | 8 cut(s) | 235, 295, 420, 469, 533, 647, 1083, 1121 |
| BseYI | CCCAGC | 1 cut(s) | 811 |
| BsgI | GTGCAG | 1 cut(s) | 116 |
| Bsh1236I | CGCG | 1 cut(s) | 955 |
| BshFI | GGCC | 3 cut(s) | 213, 295, 1299 |
| BshNI | GGYRCC | 1 cut(s) | 1087 |
| BshVI | ATCGAT | 1 cut(s) | 1254 |
| BsiHKAI | GWGCWC | 1 cut(s) | 392 |
| BsiSI | CCGG | 3 cut(s) | 157, 1300, 1314 |
| BslFI | GGGAC | 2 cut(s) | 87, 1278 |
| BslI | CCNNNNNNNGG | 4 cut(s) | 122, 157, 650, 1348 |
| BsmAI | GTCTC | 3 cut(s) | 531, 1058, 1185 |
| BsmBI | CGTCTC | 1 cut(s) | 531 |
| BsmFI | GGGAC | 2 cut(s) | 87, 1278 |
| BsmI | GAATGC | 1 cut(s) | 1323 |
| BsnI | GGCC | 3 cut(s) | 213, 295, 1299 |
| Bso31I | GGTCTC | 2 cut(s) | 1058, 1185 |
| Bsp1286I | GDGCHC | 1 cut(s) | 392 |
| Bsp143I | GATC | 6 cut(s) | 160, 490, 505, 612, 747, 775 |
| Bsp1720I | GCTNAGC | 3 cut(s) | 252, 404, 1452 |
| BspACI | CCGC | 2 cut(s) | 953, 955 |
| BspANI | GGCC | 3 cut(s) | 213, 295, 1299 |
| BspCNI | CTCAG | 5 cut(s) | 244, 256, 417, 522, 1399 |
| BspDI | ATCGAT | 1 cut(s) | 1254 |
| BspFNI | CGCG | 1 cut(s) | 955 |
| BspLI | GGNNCC | 3 cut(s) | 103, 361, 1089 |
| BspMI | ACCTGC | 1 cut(s) | 138 |
| BspPI | GGATC | 2 cut(s) | 168, 607 |
| BspQI | GCTCTTC | 1 cut(s) | 441 |
| BspT107I | GGYRCC | 1 cut(s) | 1087 |
| BspTNI | GGTCTC | 2 cut(s) | 1058, 1185 |
| BsrDI | GCAATG | 3 cut(s) | 903, 1091, 1132 |
| BsrFI | RCCGGY | 1 cut(s) | 1299 |
| BsrI | ACTGG | 2 cut(s) | 486, 1082 |
| BssAI | RCCGGY | 1 cut(s) | 1299 |
| BssECI | CCNNGG | 4 cut(s) | 688, 953, 1312, 1445 |
| BssMI | GATC | 6 cut(s) | 160, 490, 505, 612, 747, 775 |
| BssSI | CACGAG | 1 cut(s) | 1158 |
| BssT1I | CCWWGG | 1 cut(s) | 1445 |
| Bst2BI | CACGAG | 1 cut(s) | 1158 |
| Bst2UI | CCWGG | 2 cut(s) | 99, 689 |
| Bst4CI | ACNGT | 3 cut(s) | 502, 1244, 1441 |
| Bst6I | CTCTTC | 3 cut(s) | 80, 306, 441 |
| BstC8I | GCNNGC | 7 cut(s) | 33, 54, 453, 626, 888, 892, 1301 |
| BstDEI | CTNAG | 9 cut(s) | 208, 243, 252, 404, 465, 509, 699, 1407, 1452 |
| BstDSI | CCRYGG | 1 cut(s) | 953 |
| BstF5I | GGATG | 3 cut(s) | 236, 949, 1078 |
| BstFNI | CGCG | 1 cut(s) | 955 |
| BstHHI | GCGC | 3 cut(s) | 207, 705, 896 |
| BstKTI | GATC | 6 cut(s) | 163, 493, 508, 615, 750, 778 |
| BstMAI | GTCTC | 3 cut(s) | 531, 1058, 1185 |
| BstMBI | GATC | 6 cut(s) | 160, 490, 505, 612, 747, 775 |
| BstMWI | GCNNNNNNNGC | 3 cut(s) | 58, 376, 700 |
| BstNI | CCWGG | 2 cut(s) | 99, 689 |
| BstNSI | RCATGY | 3 cut(s) | 139, 628, 886 |
| BstSCI | CCNGG | 4 cut(s) | 97, 156, 687, 1312 |
| BstSFI | CTRYAG | 1 cut(s) | 771 |
| BstSLI | GKGCMC | 1 cut(s) | 392 |
| BstUI | CGCG | 1 cut(s) | 955 |
| BstV1I | GCAGC | 8 cut(s) | 235, 295, 420, 469, 533, 647, 1083, 1121 |
| BstX2I | RGATCY | 1 cut(s) | 160 |
| BstYI | RGATCY | 1 cut(s) | 160 |
| Bsu15I | ATCGAT | 1 cut(s) | 1254 |
| Bsu36I | CCTNAGG | 1 cut(s) | 243 |
| BsuRI | GGCC | 3 cut(s) | 213, 295, 1299 |
| BsuTUI | ATCGAT | 1 cut(s) | 1254 |
| BtgI | CCRYGG | 1 cut(s) | 953 |
| BtrI | CACGTC | 1 cut(s) | 1268 |
| BtsCI | GGATG | 3 cut(s) | 236, 949, 1078 |
| BtsI | GCAGTG | 1 cut(s) | 531 |
| BtsIMutI | CAGTG | 5 cut(s) | 493, 517, 531, 1089, 1446 |
| BveI | ACCTGC | 1 cut(s) | 138 |
| Cac8I | GCNNGC | 7 cut(s) | 33, 54, 453, 626, 888, 892, 1301 |
| CfoI | GCGC | 3 cut(s) | 207, 705, 896 |
| Cfr10I | RCCGGY | 1 cut(s) | 1299 |
| Cfr13I | GGNCC | 2 cut(s) | 101, 1339 |
| Cfr42I | CCGCGG | 1 cut(s) | 956 |
| ClaI | ATCGAT | 1 cut(s) | 1254 |
| CseI | GACGC | 1 cut(s) | 513 |
| Csp6I | GTAC | 4 cut(s) | 47, 571, 841, 1042 |
| CviQI | GTAC | 4 cut(s) | 47, 571, 841, 1042 |
| DdeI | CTNAG | 9 cut(s) | 208, 243, 252, 404, 465, 509, 699, 1407, 1452 |
| DpnI | GATC | 6 cut(s) | 162, 492, 507, 614, 749, 777 |
| DpnII | GATC | 6 cut(s) | 160, 490, 505, 612, 747, 775 |
| EaeI | YGGCCR | 1 cut(s) | 1297 |
| Eam1104I | CTCTTC | 3 cut(s) | 80, 306, 441 |
| EarI | CTCTTC | 3 cut(s) | 80, 306, 441 |
| Eco130I | CCWWGG | 1 cut(s) | 1445 |
| Eco147I | AGGCCT | 1 cut(s) | 213 |
| Eco31I | GGTCTC | 2 cut(s) | 1058, 1185 |
| Eco47I | GGWCC | 2 cut(s) | 101, 1339 |
| Eco57I | CTGAAG | 1 cut(s) | 1469 |
| Eco81I | CCTNAGG | 1 cut(s) | 243 |
| EcoRII | CCWGG | 2 cut(s) | 97, 687 |
| EcoT14I | CCWWGG | 1 cut(s) | 1445 |
| EcoT22I | ATGCAT | 2 cut(s) | 630, 736 |
| ErhI | CCWWGG | 1 cut(s) | 1445 |
| Esp3I | CGTCTC | 1 cut(s) | 531 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 435, 467 |
| FaqI | GGGAC | 2 cut(s) | 87, 1278 |
| FauI | CCCGC | 1 cut(s) | 948 |
| FauNDI | CATATG | 1 cut(s) | 355 |
| Fnu4HI | GCNGC | 9 cut(s) | 249, 309, 409, 483, 547, 636, 1097, 1110, 1490 |
| FokI | GGATG | 3 cut(s) | 223, 936, 1065 |
| Fsp4HI | GCNGC | 9 cut(s) | 249, 309, 409, 483, 547, 636, 1097, 1110, 1490 |
| FspBI | CTAG | 3 cut(s) | 173, 1197, 1446 |
| GlaI | GCGC | 3 cut(s) | 206, 704, 895 |
| GluI | GCNGC | 9 cut(s) | 249, 309, 409, 483, 547, 636, 1097, 1110, 1490 |
| GsaI | CCCAGC | 1 cut(s) | 815 |
| GsuI | CTGGAG | 1 cut(s) | 671 |
| HaeIII | GGCC | 3 cut(s) | 213, 295, 1299 |
| HapII | CCGG | 3 cut(s) | 157, 1300, 1314 |
| HgaI | GACGC | 1 cut(s) | 513 |
| HhaI | GCGC | 3 cut(s) | 207, 705, 896 |
| Hin6I | GCGC | 3 cut(s) | 205, 703, 894 |
| HinP1I | GCGC | 3 cut(s) | 205, 703, 894 |
| HinfI | GANTC | 3 cut(s) | 833, 846, 1019 |
| HpaII | CCGG | 3 cut(s) | 157, 1300, 1314 |
| HphI | GGTGA | 3 cut(s) | 146, 665, 970 |
| Hpy166II | GTNNAC | 2 cut(s) | 390, 927 |
| Hpy188I | TCNGA | 6 cut(s) | 125, 829, 838, 937, 1024, 1408 |
| Hpy188III | TCNNGA | 4 cut(s) | 460, 476, 779, 973 |
| Hpy8I | GTNNAC | 2 cut(s) | 390, 927 |
| Hpy99I | CGWCG | 1 cut(s) | 1244 |
| HpyAV | CCTTC | 4 cut(s) | 355, 703, 1054, 1493 |
| HpyCH4III | ACNGT | 3 cut(s) | 502, 1244, 1441 |
| HpyCH4IV | ACGT | 2 cut(s) | 990, 1267 |
| HpyF10VI | GCNNNNNNNGC | 3 cut(s) | 58, 376, 700 |
| HpyF3I | CTNAG | 9 cut(s) | 208, 243, 252, 404, 465, 509, 699, 1407, 1452 |
| HpySE526I | ACGT | 2 cut(s) | 990, 1267 |
| HspAI | GCGC | 3 cut(s) | 205, 703, 894 |
| KroI | GCCGGC | 1 cut(s) | 1299 |
| KroNI | GCCGGC | 1 cut(s) | 1301 |
| KspI | CCGCGG | 1 cut(s) | 956 |
| Kzo9I | GATC | 6 cut(s) | 160, 490, 505, 612, 747, 775 |
| LguI | GCTCTTC | 1 cut(s) | 441 |
| LmnI | GCTCC | 5 cut(s) | 223, 365, 679, 860, 1381 |
| Lsp1109I | GCAGC | 8 cut(s) | 235, 295, 420, 469, 533, 647, 1083, 1121 |
| LweI | GCATC | 5 cut(s) | 175, 721, 958, 1201, 1465 |
| MaeI | CTAG | 3 cut(s) | 173, 1197, 1446 |
| MaeII | ACGT | 2 cut(s) | 990, 1267 |
| MaeIII | GTNAC | 2 cut(s) | 496, 1268 |
| MalI | GATC | 6 cut(s) | 162, 492, 507, 614, 749, 777 |
| MboI | GATC | 6 cut(s) | 160, 490, 505, 612, 747, 775 |
| MboII | GAAGA | 9 cut(s) | 94, 97, 293, 458, 577, 757, 842, 976, 1028 |
| MfeI | CAATTG | 2 cut(s) | 918, 1182 |
| MflI | RGATCY | 1 cut(s) | 160 |
| MhlI | GDGCHC | 1 cut(s) | 392 |
| MluCI | AATT | 7 cut(s) | 337, 584, 873, 918, 1182, 1277, 1322 |
| MmeI | TCCRAC | 3 cut(s) | 882, 1017, 1392 |
| Mph1103I | ATGCAT | 2 cut(s) | 630, 736 |
| MroNI | GCCGGC | 1 cut(s) | 1299 |
| MroXI | GAANNNNTTC | 2 cut(s) | 762, 1136 |
| MslI | CAYNNNNRTG | 2 cut(s) | 200, 1055 |
| MspA1I | CMGCKG | 1 cut(s) | 955 |
| MspI | CCGG | 3 cut(s) | 157, 1300, 1314 |
| MspR9I | CCNGG | 4 cut(s) | 99, 158, 689, 1314 |
| MunI | CAATTG | 2 cut(s) | 918, 1182 |
| Mva1269I | GAATGC | 1 cut(s) | 1323 |
| MvaI | CCWGG | 2 cut(s) | 99, 689 |
| MvnI | CGCG | 1 cut(s) | 955 |
| MwoI | GCNNNNNNNGC | 3 cut(s) | 58, 376, 700 |
| NaeI | GCCGGC | 1 cut(s) | 1301 |
| NciI | CCSGG | 2 cut(s) | 158, 1314 |
| NdeI | CATATG | 1 cut(s) | 355 |
| NdeII | GATC | 6 cut(s) | 160, 490, 505, 612, 747, 775 |
| NgoMIV | GCCGGC | 1 cut(s) | 1299 |
| NlaIV | GGNNCC | 3 cut(s) | 103, 361, 1089 |
| NmuCI | GTSAC | 1 cut(s) | 1268 |
| NsiI | ATGCAT | 2 cut(s) | 630, 736 |
| NspI | RCATGY | 3 cut(s) | 139, 628, 886 |
| PaeI | GCATGC | 1 cut(s) | 628 |
| PceI | AGGCCT | 1 cut(s) | 213 |
| PciI | ACATGT | 1 cut(s) | 135 |
| PciSI | GCTCTTC | 1 cut(s) | 441 |
| PctI | GAATGC | 1 cut(s) | 1323 |
| PdiI | GCCGGC | 1 cut(s) | 1301 |
| PdmI | GAANNNNTTC | 2 cut(s) | 762, 1136 |
| PfeI | GAWTC | 3 cut(s) | 833, 846, 1019 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 1348 |
| PkrI | GCNGC | 9 cut(s) | 250, 310, 410, 484, 548, 637, 1098, 1111, 1491 |
| PscI | ACATGT | 1 cut(s) | 135 |
| Psp6I | CCWGG | 2 cut(s) | 97, 687 |
| PspFI | CCCAGC | 1 cut(s) | 811 |
| PspGI | CCWGG | 2 cut(s) | 97, 687 |
| PspN4I | GGNNCC | 3 cut(s) | 103, 361, 1089 |
| PspPI | GGNCC | 2 cut(s) | 101, 1339 |
| PsuI | RGATCY | 1 cut(s) | 160 |
| RsaI | GTAC | 4 cut(s) | 48, 572, 842, 1043 |
| RsaNI | GTAC | 4 cut(s) | 47, 571, 841, 1042 |
| RseI | CAYNNNNRTG | 2 cut(s) | 200, 1055 |
| SacII | CCGCGG | 1 cut(s) | 956 |
| SapI | GCTCTTC | 1 cut(s) | 441 |
| SatI | GCNGC | 9 cut(s) | 249, 309, 409, 483, 547, 636, 1097, 1110, 1490 |
| Sau3AI | GATC | 6 cut(s) | 160, 490, 505, 612, 747, 775 |
| Sau96I | GGNCC | 2 cut(s) | 101, 1339 |
| ScaI | AGTACT | 1 cut(s) | 1043 |
| ScrFI | CCNGG | 4 cut(s) | 99, 158, 689, 1314 |
| SduI | GDGCHC | 1 cut(s) | 392 |
| SfaNI | GCATC | 5 cut(s) | 175, 721, 958, 1201, 1465 |
| SfcI | CTRYAG | 1 cut(s) | 771 |
| Sfr303I | CCGCGG | 1 cut(s) | 956 |
| SgrBI | CCGCGG | 1 cut(s) | 956 |
| SinI | GGWCC | 2 cut(s) | 101, 1339 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 200, 1055 |
| SmlI | CTYRAG | 1 cut(s) | 395 |
| SmoI | CTYRAG | 1 cut(s) | 395 |
| SphI | GCATGC | 1 cut(s) | 628 |
| Sse9I | AATT | 7 cut(s) | 337, 584, 873, 918, 1182, 1277, 1322 |
| SseBI | AGGCCT | 1 cut(s) | 213 |
| SsiI | CCGC | 2 cut(s) | 953, 955 |
| SspMI | CTAG | 3 cut(s) | 173, 1197, 1446 |
| StuI | AGGCCT | 1 cut(s) | 213 |
| StyD4I | CCNGG | 4 cut(s) | 97, 156, 687, 1312 |
| StyI | CCWWGG | 1 cut(s) | 1445 |
| TaaI | ACNGT | 3 cut(s) | 502, 1244, 1441 |
| TaiI | ACGT | 2 cut(s) | 993, 1270 |
| TaqI | TCGA | 4 cut(s) | 169, 849, 1239, 1254 |
| TasI | AATT | 7 cut(s) | 337, 584, 873, 918, 1182, 1277, 1322 |
| TatI | WGTACW | 3 cut(s) | 46, 570, 1041 |
| TfiI | GAWTC | 3 cut(s) | 833, 846, 1019 |
| TscAI | CASTG | 5 cut(s) | 493, 517, 538, 1089, 1446 |
| TseFI | GTSAC | 1 cut(s) | 1268 |
| TseI | GCWGC | 9 cut(s) | 248, 308, 408, 482, 546, 635, 1096, 1109, 1489 |
| Tsp45I | GTSAC | 1 cut(s) | 1268 |
| TspDTI | ATGAA | 9 cut(s) | 225, 649, 722, 771, 808, 977, 1067, 1213, 1355 |
| TspGWI | ACGGA | 1 cut(s) | 1436 |
| TspRI | CASTG | 5 cut(s) | 493, 517, 538, 1089, 1446 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 1348 |
| VneI | GTGCAC | 1 cut(s) | 388 |
| VpaK11BI | GGWCC | 2 cut(s) | 101, 1339 |
| XapI | RAATTY | 2 cut(s) | 337, 584 |
| XceI | RCATGY | 3 cut(s) | 139, 628, 886 |
| XmaJI | CCTAGG | 1 cut(s) | 1445 |
| XmnI | GAANNNNTTC | 2 cut(s) | 762, 1136 |
| XspI | CTAG | 3 cut(s) | 173, 1197, 1446 |
| ZrmI | AGTACT | 1 cut(s) | 1043 |
| Zsp2I | ATGCAT | 2 cut(s) | 630, 736 |