Cytochrome p450
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc36I | ACCTGC | 2 cut(s) | 590, 729 |
| AccBSI | CCGCTC | 1 cut(s) | 284 |
| AccII | CGCG | 1 cut(s) | 1197 |
| AciI | CCGC | 5 cut(s) | 276, 284, 627, 713, 935 |
| AclI | AACGTT | 1 cut(s) | 1287 |
| AclWI | GGATC | 1 cut(s) | 204 |
| AcoI | YGGCCR | 1 cut(s) | 285 |
| AcuI | CTGAAG | 2 cut(s) | 885, 1505 |
| AcyI | GRCGYC | 1 cut(s) | 519 |
| AdeI | CACNNNGTG | 1 cut(s) | 1384 |
| AfaI | GTAC | 5 cut(s) | 236, 608, 678, 1263, 1377 |
| AfiI | CCNNNNNNNGG | 5 cut(s) | 158, 193, 341, 686, 858 |
| AflIII | ACRYGT | 4 cut(s) | 918, 1195, 1209, 1431 |
| AgsI | TTSAA | 7 cut(s) | 8, 466, 512, 664, 803, 895, 1482 |
| AjnI | CCWGG | 1 cut(s) | 133 |
| AluBI | AGCT | 5 cut(s) | 292, 375, 381, 421, 1396 |
| AluI | AGCT | 5 cut(s) | 292, 375, 381, 421, 1396 |
| Alw26I | GTCTC | 2 cut(s) | 274, 1221 |
| AlwI | GGATC | 1 cut(s) | 204 |
| Ama87I | CYCGRG | 1 cut(s) | 566 |
| AoxI | GGCC | 5 cut(s) | 247, 285, 849, 927, 1485 |
| ApeKI | GCWGC | 2 cut(s) | 162, 1145 |
| ArsI | GACNNNNNNTTYG | 4 cut(s) | 302, 334, 605, 637 |
| AspLEI | GCGC | 1 cut(s) | 967 |
| AspS9I | GGNCC | 2 cut(s) | 137, 1485 |
| AsuHPI | GGTGA | 1 cut(s) | 236 |
| AvaI | CYCGRG | 1 cut(s) | 566 |
| AvaII | GGWCC | 1 cut(s) | 137 |
| BanII | GRGCYC | 1 cut(s) | 732 |
| BauI | CACGAG | 1 cut(s) | 279 |
| BbsI | GAAGAC | 1 cut(s) | 1345 |
| BbvCI | CCTCAGC | 1 cut(s) | 931 |
| BbvI | GCAGC | 2 cut(s) | 174, 1157 |
| BccI | CCATC | 4 cut(s) | 20, 583, 1157, 1163 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 405, 439 |
| BciT130I | CCWGG | 1 cut(s) | 135 |
| BcoDI | GTCTC | 2 cut(s) | 274, 1221 |
| BfaI | CTAG | 2 cut(s) | 209, 668 |
| BfoI | RGCGCY | 1 cut(s) | 968 |
| BfuAI | ACCTGC | 2 cut(s) | 590, 729 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 848 |
| BisI | GCNGC | 3 cut(s) | 163, 285, 1146 |
| BlpI | GCTNAGC | 1 cut(s) | 1392 |
| BlsI | GCNGC | 3 cut(s) | 164, 286, 1147 |
| Bme1390I | CCNGG | 1 cut(s) | 135 |
| Bme18I | GGWCC | 1 cut(s) | 137 |
| BmeT110I | CYCGRG | 1 cut(s) | 566 |
| BmgT120I | GGNCC | 2 cut(s) | 137, 1485 |
| BmrFI | CCNGG | 1 cut(s) | 135 |
| BmsI | GCATC | 6 cut(s) | 82, 802, 805, 829, 994, 1237 |
| BpiI | GAAGAC | 1 cut(s) | 1345 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 380, 412 |
| BpmI | CTGGAG | 1 cut(s) | 236 |
| Bpu10I | CCTNAGC | 1 cut(s) | 931 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 1392 |
| BpuEI | CTTGAG | 2 cut(s) | 259, 397 |
| BsaHI | GRCGYC | 1 cut(s) | 519 |
| BsaI | GGTCTC | 1 cut(s) | 1221 |
| BsaJI | CCNNGG | 2 cut(s) | 192, 807 |
| Bsc4I | CCNNNNNNNGG | 5 cut(s) | 158, 193, 341, 686, 858 |
| Bse3DI | GCAATG | 1 cut(s) | 534 |
| BseBI | CCWGG | 1 cut(s) | 135 |
| BseDI | CCNNGG | 2 cut(s) | 192, 807 |
| BseGI | GGATG | 4 cut(s) | 594, 817, 985, 1114 |
| BseLI | CCNNNNNNNGG | 5 cut(s) | 158, 193, 341, 686, 858 |
| BseMI | GCAATG | 1 cut(s) | 534 |
| BseMII | CTCAG | 4 cut(s) | 378, 945, 1406, 1449 |
| BseRI | GAGGAG | 4 cut(s) | 490, 632, 646, 1475 |
| BseX3I | CGGCCG | 1 cut(s) | 285 |
| BseXI | GCAGC | 2 cut(s) | 174, 1157 |
| Bsh1236I | CGCG | 1 cut(s) | 1197 |
| Bsh1285I | CGRYCG | 1 cut(s) | 288 |
| BshFI | GGCC | 5 cut(s) | 249, 287, 851, 929, 1487 |
| BsiEI | CGRYCG | 1 cut(s) | 288 |
| BsiHKCI | CYCGRG | 1 cut(s) | 566 |
| BsiSI | CCGG | 1 cut(s) | 586 |
| BslI | CCNNNNNNNGG | 5 cut(s) | 158, 193, 341, 686, 858 |
| BsmAI | GTCTC | 2 cut(s) | 274, 1221 |
| BsmI | GAATGC | 1 cut(s) | 1359 |
| BsnI | GGCC | 5 cut(s) | 249, 287, 851, 929, 1487 |
| Bso31I | GGTCTC | 1 cut(s) | 1221 |
| BsoBI | CYCGRG | 1 cut(s) | 566 |
| Bsp1286I | GDGCHC | 1 cut(s) | 732 |
| Bsp143I | GATC | 2 cut(s) | 196, 453 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 1392 |
| Bsp19I | CCATGG | 1 cut(s) | 192 |
| BspACI | CCGC | 5 cut(s) | 276, 284, 627, 713, 935 |
| BspANI | GGCC | 5 cut(s) | 249, 287, 851, 929, 1487 |
| BspCNI | CTCAG | 4 cut(s) | 379, 944, 1405, 1450 |
| BspFNI | CGCG | 1 cut(s) | 1197 |
| BspHI | TCATGA | 1 cut(s) | 978 |
| BspMI | ACCTGC | 2 cut(s) | 590, 729 |
| BspPI | GGATC | 1 cut(s) | 204 |
| BspTNI | GGTCTC | 1 cut(s) | 1221 |
| BsrBI | CCGCTC | 1 cut(s) | 284 |
| BsrDI | GCAATG | 1 cut(s) | 534 |
| BssECI | CCNNGG | 2 cut(s) | 192, 807 |
| BssMI | GATC | 2 cut(s) | 196, 453 |
| BssNI | GRCGYC | 1 cut(s) | 519 |
| BssSI | CACGAG | 1 cut(s) | 279 |
| BssT1I | CCWWGG | 2 cut(s) | 192, 807 |
| Bst2BI | CACGAG | 1 cut(s) | 279 |
| Bst2UI | CCWGG | 1 cut(s) | 135 |
| Bst4CI | ACNGT | 6 cut(s) | 224, 262, 955, 1165, 1323, 1372 |
| Bst6I | CTCTTC | 2 cut(s) | 277, 477 |
| BstACI | GRCGYC | 1 cut(s) | 519 |
| BstC8I | GCNNGC | 6 cut(s) | 61, 82, 86, 428, 849, 1500 |
| BstDEI | CTNAG | 5 cut(s) | 387, 440, 931, 1392, 1458 |
| BstDSI | CCRYGG | 1 cut(s) | 192 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 339 |
| BstF5I | GGATG | 4 cut(s) | 594, 817, 985, 1114 |
| BstFNI | CGCG | 1 cut(s) | 1197 |
| BstH2I | RGCGCY | 1 cut(s) | 968 |
| BstHHI | GCGC | 1 cut(s) | 967 |
| BstKTI | GATC | 2 cut(s) | 199, 456 |
| BstMAI | GTCTC | 2 cut(s) | 274, 1221 |
| BstMBI | GATC | 2 cut(s) | 196, 453 |
| BstMCI | CGRYCG | 1 cut(s) | 288 |
| BstMWI | GCNNNNNNNGC | 4 cut(s) | 284, 427, 721, 848 |
| BstNI | CCWGG | 1 cut(s) | 135 |
| BstNSI | RCATGY | 5 cut(s) | 88, 415, 430, 922, 1435 |
| BstSCI | CCNGG | 1 cut(s) | 133 |
| BstUI | CGCG | 1 cut(s) | 1197 |
| BstV1I | GCAGC | 2 cut(s) | 174, 1157 |
| BstV2I | GAAGAC | 1 cut(s) | 1345 |
| BstX2I | RGATCY | 1 cut(s) | 196 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 1239 |
| BstYI | RGATCY | 1 cut(s) | 196 |
| BstZI | CGGCCG | 1 cut(s) | 285 |
| BsuRI | GGCC | 5 cut(s) | 249, 287, 851, 929, 1487 |
| BtgI | CCRYGG | 1 cut(s) | 192 |
| BtsCI | GGATG | 4 cut(s) | 594, 817, 985, 1114 |
| BtsIMutI | CAGTG | 2 cut(s) | 546, 1161 |
| BveI | ACCTGC | 2 cut(s) | 590, 729 |
| Cac8I | GCNNGC | 6 cut(s) | 61, 82, 86, 428, 849, 1500 |
| CciI | TCATGA | 1 cut(s) | 978 |
| CfoI | GCGC | 1 cut(s) | 967 |
| Cfr13I | GGNCC | 2 cut(s) | 137, 1485 |
| CseI | GACGC | 2 cut(s) | 527, 540 |
| Csp6I | GTAC | 5 cut(s) | 235, 607, 677, 1262, 1376 |
| CviQI | GTAC | 5 cut(s) | 235, 607, 677, 1262, 1376 |
| DdeI | CTNAG | 5 cut(s) | 387, 440, 931, 1392, 1458 |
| DpnI | GATC | 2 cut(s) | 198, 455 |
| DpnII | GATC | 2 cut(s) | 196, 453 |
| DraIII | CACNNNGTG | 1 cut(s) | 1384 |
| EaeI | YGGCCR | 1 cut(s) | 285 |
| EagI | CGGCCG | 1 cut(s) | 285 |
| Eam1104I | CTCTTC | 2 cut(s) | 277, 477 |
| EarI | CTCTTC | 2 cut(s) | 277, 477 |
| EclXI | CGGCCG | 1 cut(s) | 285 |
| Eco130I | CCWWGG | 2 cut(s) | 192, 807 |
| Eco147I | AGGCCT | 1 cut(s) | 249 |
| Eco24I | GRGCYC | 1 cut(s) | 732 |
| Eco31I | GGTCTC | 1 cut(s) | 1221 |
| Eco47I | GGWCC | 1 cut(s) | 137 |
| Eco52I | CGGCCG | 1 cut(s) | 285 |
| Eco57I | CTGAAG | 2 cut(s) | 885, 1505 |
| Eco88I | CYCGRG | 1 cut(s) | 566 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 339 |
| EcoRII | CCWGG | 1 cut(s) | 133 |
| EcoT14I | CCWWGG | 2 cut(s) | 192, 807 |
| EcoT22I | ATGCAT | 1 cut(s) | 90 |
| EcoT38I | GRGCYC | 1 cut(s) | 732 |
| ErhI | CCWWGG | 2 cut(s) | 192, 807 |
| FauI | CCCGC | 1 cut(s) | 620 |
| Fnu4HI | GCNGC | 3 cut(s) | 163, 285, 1146 |
| FokI | GGATG | 4 cut(s) | 601, 824, 972, 1101 |
| FriOI | GRGCYC | 1 cut(s) | 732 |
| Fsp4HI | GCNGC | 3 cut(s) | 163, 285, 1146 |
| FspBI | CTAG | 2 cut(s) | 209, 668 |
| GlaI | GCGC | 1 cut(s) | 966 |
| GluI | GCNGC | 3 cut(s) | 163, 285, 1146 |
| GsuI | CTGGAG | 1 cut(s) | 236 |
| HaeII | RGCGCY | 1 cut(s) | 968 |
| HaeIII | GGCC | 5 cut(s) | 249, 287, 851, 929, 1487 |
| HapII | CCGG | 1 cut(s) | 586 |
| HgaI | GACGC | 2 cut(s) | 527, 540 |
| HhaI | GCGC | 1 cut(s) | 967 |
| Hin1I | GRCGYC | 1 cut(s) | 519 |
| Hin6I | GCGC | 1 cut(s) | 965 |
| HinP1I | GCGC | 1 cut(s) | 965 |
| HincII | GTYRAC | 3 cut(s) | 46, 206, 754 |
| HindII | GTYRAC | 3 cut(s) | 46, 206, 754 |
| HindIII | AAGCTT | 2 cut(s) | 373, 419 |
| HinfI | GANTC | 5 cut(s) | 869, 882, 1055, 1315, 1492 |
| HpaI | GTTAAC | 2 cut(s) | 46, 754 |
| HpaII | CCGG | 1 cut(s) | 586 |
| HphI | GGTGA | 1 cut(s) | 236 |
| Hpy166II | GTNNAC | 5 cut(s) | 46, 206, 754, 1207, 1378 |
| Hpy188I | TCNGA | 9 cut(s) | 141, 865, 874, 916, 973, 1038, 1060, 1314, 1524 |
| Hpy188III | TCNNGA | 7 cut(s) | 23, 496, 512, 568, 979, 1222, 1424 |
| Hpy8I | GTNNAC | 5 cut(s) | 46, 206, 754, 1207, 1378 |
| Hpy99I | CGWCG | 1 cut(s) | 534 |
| HpyAV | CCTTC | 5 cut(s) | 335, 739, 820, 1090, 1529 |
| HpyCH4III | ACNGT | 6 cut(s) | 224, 262, 955, 1165, 1323, 1372 |
| HpyCH4IV | ACGT | 3 cut(s) | 1209, 1287, 1342 |
| HpyF10VI | GCNNNNNNNGC | 4 cut(s) | 284, 427, 721, 848 |
| HpyF3I | CTNAG | 5 cut(s) | 387, 440, 931, 1392, 1458 |
| HpySE526I | ACGT | 3 cut(s) | 1209, 1287, 1342 |
| Hsp92I | GRCGYC | 1 cut(s) | 519 |
| HspAI | GCGC | 1 cut(s) | 965 |
| KspAI | GTTAAC | 2 cut(s) | 46, 754 |
| Kzo9I | GATC | 2 cut(s) | 196, 453 |
| Lsp1109I | GCAGC | 2 cut(s) | 174, 1157 |
| LweI | GCATC | 6 cut(s) | 82, 802, 805, 829, 994, 1237 |
| MaeI | CTAG | 2 cut(s) | 209, 668 |
| MaeII | ACGT | 3 cut(s) | 1209, 1287, 1342 |
| MaeIII | GTNAC | 4 cut(s) | 126, 224, 1122, 1191 |
| MalI | GATC | 2 cut(s) | 198, 455 |
| MbiI | CCGCTC | 1 cut(s) | 284 |
| MboI | GATC | 2 cut(s) | 196, 453 |
| MfeI | CAATTG | 1 cut(s) | 49 |
| MflI | RGATCY | 1 cut(s) | 196 |
| MhlI | GDGCHC | 1 cut(s) | 732 |
| MluCI | AATT | 5 cut(s) | 49, 316, 434, 1070, 1525 |
| MluI | ACGCGT | 1 cut(s) | 1195 |
| MmeI | TCCRAC | 3 cut(s) | 894, 1330, 1428 |
| Mph1103I | ATGCAT | 1 cut(s) | 90 |
| MseI | TTAA | 3 cut(s) | 45, 315, 753 |
| MslI | CAYNNNNRTG | 1 cut(s) | 923 |
| MspA1I | CMGCKG | 1 cut(s) | 935 |
| MspI | CCGG | 1 cut(s) | 586 |
| MspR9I | CCNGG | 1 cut(s) | 135 |
| MunI | CAATTG | 1 cut(s) | 49 |
| Mva1269I | GAATGC | 1 cut(s) | 1359 |
| MvaI | CCWGG | 1 cut(s) | 135 |
| MvnI | CGCG | 1 cut(s) | 1197 |
| MwoI | GCNNNNNNNGC | 4 cut(s) | 284, 427, 721, 848 |
| NcoI | CCATGG | 1 cut(s) | 192 |
| NdeII | GATC | 2 cut(s) | 196, 453 |
| NmeAIII | GCCGAG | 1 cut(s) | 313 |
| NmuCI | GTSAC | 2 cut(s) | 224, 1191 |
| NsiI | ATGCAT | 1 cut(s) | 90 |
| NspI | RCATGY | 5 cut(s) | 88, 415, 430, 922, 1435 |
| PaeI | GCATGC | 2 cut(s) | 88, 430 |
| PagI | TCATGA | 1 cut(s) | 978 |
| PceI | AGGCCT | 1 cut(s) | 249 |
| PciI | ACATGT | 2 cut(s) | 918, 1431 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 404 |
| PctI | GAATGC | 1 cut(s) | 1359 |
| PfeI | GAWTC | 5 cut(s) | 869, 882, 1055, 1315, 1492 |
| PflFI | GACNNNGTC | 1 cut(s) | 554 |
| PkrI | GCNGC | 3 cut(s) | 164, 286, 1147 |
| PscI | ACATGT | 2 cut(s) | 918, 1431 |
| Psp1406I | AACGTT | 1 cut(s) | 1287 |
| Psp6I | CCWGG | 1 cut(s) | 133 |
| PspGI | CCWGG | 1 cut(s) | 133 |
| PspPI | GGNCC | 2 cut(s) | 137, 1485 |
| PsuI | RGATCY | 1 cut(s) | 196 |
| PsyI | GACNNNGTC | 1 cut(s) | 554 |
| RsaI | GTAC | 5 cut(s) | 236, 608, 678, 1263, 1377 |
| RsaNI | GTAC | 5 cut(s) | 235, 607, 677, 1262, 1376 |
| RseI | CAYNNNNRTG | 1 cut(s) | 923 |
| SaqAI | TTAA | 3 cut(s) | 45, 315, 753 |
| SatI | GCNGC | 3 cut(s) | 163, 285, 1146 |
| Sau3AI | GATC | 2 cut(s) | 196, 453 |
| Sau96I | GGNCC | 2 cut(s) | 137, 1485 |
| ScrFI | CCNGG | 1 cut(s) | 135 |
| SduI | GDGCHC | 1 cut(s) | 732 |
| SfaNI | GCATC | 6 cut(s) | 82, 802, 805, 829, 994, 1237 |
| SinI | GGWCC | 1 cut(s) | 137 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 923 |
| SmlI | CTYRAG | 2 cut(s) | 238, 376 |
| SmoI | CTYRAG | 2 cut(s) | 238, 376 |
| SphI | GCATGC | 2 cut(s) | 88, 430 |
| Sse9I | AATT | 5 cut(s) | 49, 316, 434, 1070, 1525 |
| SseBI | AGGCCT | 1 cut(s) | 249 |
| SsiI | CCGC | 5 cut(s) | 276, 284, 627, 713, 935 |
| SspMI | CTAG | 2 cut(s) | 209, 668 |
| StuI | AGGCCT | 1 cut(s) | 249 |
| StyD4I | CCNGG | 1 cut(s) | 133 |
| StyI | CCWWGG | 2 cut(s) | 192, 807 |
| TaaI | ACNGT | 6 cut(s) | 224, 262, 955, 1165, 1323, 1372 |
| TaiI | ACGT | 3 cut(s) | 1212, 1290, 1345 |
| TaqI | TCGA | 5 cut(s) | 432, 529, 885, 1152, 1167 |
| TasI | AATT | 5 cut(s) | 49, 316, 434, 1070, 1525 |
| TatI | WGTACW | 2 cut(s) | 234, 606 |
| TauI | GCSGC | 1 cut(s) | 287 |
| TfiI | GAWTC | 5 cut(s) | 869, 882, 1055, 1315, 1492 |
| Tru1I | TTAA | 3 cut(s) | 45, 315, 753 |
| Tru9I | TTAA | 3 cut(s) | 45, 315, 753 |
| TscAI | CASTG | 2 cut(s) | 553, 1168 |
| TseFI | GTSAC | 2 cut(s) | 224, 1191 |
| TseI | GCWGC | 2 cut(s) | 162, 1145 |
| Tsp45I | GTSAC | 2 cut(s) | 224, 1191 |
| TspDTI | ATGAA | 4 cut(s) | 354, 665, 758, 1013 |
| TspGWI | ACGGA | 1 cut(s) | 1079 |
| TspRI | CASTG | 2 cut(s) | 553, 1168 |
| Tth111I | GACNNNGTC | 1 cut(s) | 554 |
| VpaK11BI | GGWCC | 1 cut(s) | 137 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 339 |
| XceI | RCATGY | 5 cut(s) | 88, 415, 430, 922, 1435 |
| XspI | CTAG | 2 cut(s) | 209, 668 |
| Zsp2I | ATGCAT | 1 cut(s) | 90 |