Rh5AG289800

Cytochrome p450

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0000398
Physical Location & Seq
Reverse (-)
39634408 .. 39635295
888 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG289800.1

Sequence Viewer

Length: 888 bp
ATGATGGTTTGGAATTGGGCAGTAATAATTGCATTGCTTGCTGTTGTTTATGTTCTGGAACCATGGAGAAGCAAGAAGAAGAGGTTACCTCCTGGTCCAAGAGGGTTTCCTATTTTCGGAAGCCTCAACTTGTTAGGGGAGTTTCCTTACAAAGACCTACACAGACTGGCTCAAAAGTATGGTGACATCATGCACATGCGTTTAGGTCTCGTGTCTGCCATCGTCGTCTCCTCCCCTCAAGCAGCCGAGCTCTTCCTCAAGACCCACGACCTTGTTTTCGCAAGCAGGCCACCTCACGAAGGCGCAAAGCACATCTCTTTCGGGCAGAGGAACTTGACCTTTTCCAAGTATGGCTCTTATTGGCGAGACATGCGCAAGATGTGCACCCTCGAGTTGCTCAGCAACCACAAAATCAATTCTTTCAAGGAAATGAGGAGAGAAGAGATTGTCCTTTTCAAAGAGGCCATTAAAGAGGCAGCTGCAACCAGCCACGAACCAGTCGATCTCAGCTCTATGGTGTCATCTCTTAGTGCGGATATGAGCTGCAGGATGGTGTTTGGAAAGAAGTACACGGATAAAGAATTCGACGACAAGGGTTTCAAGTCTGTGATTCAACAAGGCAAACAATTAGCAGCTGCACCTAACTTGGGCGATTACATCCCTTGTATTGCTCCACTTGACCTCCAAGGTTTCACTAAGCGCATGAAGGCTATTAACAAGGTGTTTGATGACTTTTTCGAGAAGATTATTGATGAACATCTCCAGTCGAATGATGAGGGAAAGACGAAGGACTTTGTTGATGTAATGCTCAGCTTTATGGGGACTCAAGAATCTGAATACCTGATTGAACGCTCCAATATCAAAGCAATCATTTTGGTAAGTAACTAG

Protein Analysis

295

Amino Acids

33.63

Weight (kDa)

8.93

Isoelectric Point (pI)

40.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 30 - 290 3.5e-36 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000398)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g21960 FvH4_3g24140 FvH4_3g24150 FvH4_7g09230 FvH4_7g09260
malus_domestica MD00G1169700.v1.1 MD00G1169800.v1.1 MD01G1016400.v1.1 MD03G1188000.v1.1 MD03G1188100.v1.1 MD11G1219700.v1.1 MD11G1219900.v1.1 MD11G1220200.v1.1 MD11G1220400.v1.1 MD11G1220600.v1.1
prunus_persica Prupe.2G006500_v2.0.a1 Prupe.4G235500_v2.0.a1 Prupe.4G235600_v2.0.a1 Prupe.4G235700_v2.0.a1 Prupe.4G235700_v2.0.a1 Prupe.4G235800_v2.0.a1 Prupe.4G235900_v2.0.a1 Prupe.6G176500_v2.0.a1
pyrus_communis pycom01g04750 pycom03g14060 pycom11g19360 pycom11g19370 pycom11g19380 pycom11g19390 pycom11g19400 pycom11g19460
rosa_chinensis RchiOBHm_Chr1g0345321 RchiOBHm_Chr1g0345511 RchiOBHm_Chr1g0345781 RchiOBHm_Chr2g0115981 RchiOBHm_Chr5g0043151 RchiOBHm_Chr5g0043161 RchiOBHm_Chr5g0043901 RchiOBHm_Chr6g0274841
rosa_laevigata RLG00000013516 RLG00000018229 RLG00000028840 RLG00000028923 RLG00000034169 RLG00000034223
rosa_multiflora Rmu_co8474237.1_g000001 Rmu_sc0000698.1_g000004 Rmu_sc0000698.1_g000021 Rmu_sc0000698.1_g000023 Rmu_sc0000698.1_g000056 Rmu_sc0000698.1_g000057 Rmu_sc0000698.1_g000122 Rmu_sc0000698.1_g000123 Rmu_sc0000698.1_g000124 Rmu_sc0001292.1_g000005 Rmu_sc0001292.1_g000006 Rmu_sc0001349.1_g000002 Rmu_sc0001692.1_g000035 Rmu_sc0002079.1_g000035 Rmu_sc0002079.1_g000045 Rmu_sc0002329.1_g000017 Rmu_sc0008148.1_g000056 Rmu_sc0008148.1_g000057 Rmu_sc0008148.1_g000058 Rmu_sc0008148.1_g000062 Rmu_sc0008611.1_g000010 Rmu_sc0009227.1_g000007
rosa_roxburghii Rroxscaffold_1G00011260 Rroxscaffold_1G00037590 Rroxscaffold_2G00127610 Rroxscaffold_4G00309120 Rroxscaffold_7G00193880
rosa_rugosa Rorug01G0177400 Rorug02G0202400 Rorug05G0204600 Rorug05G0209000 Rorug06G0085100 Rorug06G0085200
rosa_samantha Rh1BG154400 Rh1DG186600 Rh2CG265200 Rh2DG267000 Rh5AG289700 Rh5AG289800 Rh5AG290000 Rh5AG293200 Rh5CG326500 Rh5DG305700 Rh5DG305800 Rh5DG310100 Rh6BG204200 Rh6CG204500 Rh6DG195700
rosa_wichuraiana Rw1G016100 Rw1G016470 Rw2G020290 Rw5G026830 Rw5G026910 Rw5G026920 Rw5G026930 Rw6G017460

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 374
AciI CCGC 1 cut(s) 533
AcsI RAATTY 1 cut(s) 581
AfaI GTAC 1 cut(s) 569
AfiI CCNNNNNNNGG 3 cut(s) 116, 299, 647
AgsI TTSAA 5 cut(s) 424, 457, 601, 614, 848
AjnI CCWGG 1 cut(s) 91
AluBI AGCT 6 cut(s) 250, 479, 510, 543, 635, 813
AluI AGCT 6 cut(s) 250, 479, 510, 543, 635, 813
Alw21I GWGCWC 2 cut(s) 252, 386
Alw26I GTCTC 3 cut(s) 212, 232, 360
Alw44I GTGCAC 1 cut(s) 382
Ama87I CYCGRG 1 cut(s) 389
AoxI GGCC 2 cut(s) 287, 462
ApaLI GTGCAC 1 cut(s) 382
ApeKI GCWGC 6 cut(s) 242, 476, 479, 543, 632, 635
ApoI RAATTY 1 cut(s) 581
ArsI GACNNNNNNTTYG 2 cut(s) 260, 292
AspLEI GCGC 3 cut(s) 305, 375, 702
AspS9I GGNCC 1 cut(s) 95
AsuHPI GGTGA 1 cut(s) 194
AvaI CYCGRG 1 cut(s) 389
AvaII GGWCC 1 cut(s) 95
BaeGI GKGCMC 1 cut(s) 386
BanII GRGCYC 1 cut(s) 252
BauI CACGAG 1 cut(s) 209
Bbv12I GWGCWC 2 cut(s) 252, 386
BbvI GCAGC 6 cut(s) 254, 466, 488, 530, 622, 644
BccI CCATC 2 cut(s) 227, 544
BciT130I CCWGG 1 cut(s) 93
BcoDI GTCTC 3 cut(s) 212, 232, 360
BfaI CTAG 1 cut(s) 886
BfmI CTRYAG 1 cut(s) 544
BisI GCNGC 6 cut(s) 243, 477, 480, 544, 633, 636
BlpI GCTNAGC 2 cut(s) 398, 809
BlsI GCNGC 6 cut(s) 244, 478, 481, 545, 634, 637
Bme1390I CCNGG 1 cut(s) 93
Bme18I GGWCC 1 cut(s) 95
BmeT110I CYCGRG 1 cut(s) 389
BmgT120I GGNCC 1 cut(s) 95
BmiI GGNNCC 1 cut(s) 60
BmrFI CCNGG 1 cut(s) 93
BplI GAGNNNNNCTC 2 cut(s) 73, 105
BpmI CTGGAG 1 cut(s) 746
Bpu1102I GCTNAGC 2 cut(s) 398, 809
BpuEI CTTGAG 3 cut(s) 222, 242, 810
BsaBI GATNNNNATC 1 cut(s) 756
BsaI GGTCTC 1 cut(s) 212
BsaJI CCNNGG 2 cut(s) 62, 685
BsaXI ACNNNNNCTCC 2 cut(s) 666, 696
Bsc4I CCNNNNNNNGG 3 cut(s) 116, 299, 647
Bse1I ACTGG 3 cut(s) 171, 497, 763
Bse3DI GCAATG 1 cut(s) 32
Bse8I GATNNNNATC 1 cut(s) 756
BseBI CCWGG 1 cut(s) 93
BseDI CCNNGG 2 cut(s) 62, 685
BseGI GGATG 2 cut(s) 555, 657
BseJI GATNNNNATC 1 cut(s) 756
BseLI CCNNNNNNNGG 3 cut(s) 116, 299, 647
BseMI GCAATG 1 cut(s) 32
BseMII CTCAG 3 cut(s) 412, 520, 823
BseNI ACTGG 3 cut(s) 171, 497, 763
BseRI GAGGAG 2 cut(s) 220, 448
BseSI GKGCMC 1 cut(s) 386
BseXI GCAGC 6 cut(s) 254, 466, 488, 530, 622, 644
BsgI GTGCAG 1 cut(s) 621
BshFI GGCC 2 cut(s) 289, 464
BsiHKAI GWGCWC 2 cut(s) 252, 386
BsiHKCI CYCGRG 1 cut(s) 389
BslFI GGGAC 1 cut(s) 835
BslI CCNNNNNNNGG 3 cut(s) 116, 299, 647
BsmAI GTCTC 3 cut(s) 212, 232, 360
BsmBI CGTCTC 1 cut(s) 232
BsmFI GGGAC 1 cut(s) 835
BsnI GGCC 2 cut(s) 289, 464
Bso31I GGTCTC 1 cut(s) 212
BsoBI CYCGRG 1 cut(s) 389
Bsp1286I GDGCHC 2 cut(s) 252, 386
Bsp143I GATC 1 cut(s) 502
Bsp1720I GCTNAGC 2 cut(s) 398, 809
Bsp19I CCATGG 1 cut(s) 62
BspACI CCGC 1 cut(s) 533
BspANI GGCC 2 cut(s) 289, 464
BspCNI CTCAG 3 cut(s) 411, 519, 822
BspLI GGNNCC 1 cut(s) 60
BspMAI CTGCAG 1 cut(s) 548
BspQI GCTCTTC 1 cut(s) 257
BspTNI GGTCTC 1 cut(s) 212
BsrDI GCAATG 1 cut(s) 32
BsrI ACTGG 3 cut(s) 171, 497, 763
BssECI CCNNGG 2 cut(s) 62, 685
BssMI GATC 1 cut(s) 502
BssSI CACGAG 1 cut(s) 209
BssT1I CCWWGG 2 cut(s) 62, 685
Bst2BI CACGAG 1 cut(s) 209
Bst2UI CCWGG 1 cut(s) 93
Bst6I CTCTTC 3 cut(s) 74, 257, 435
BstAPI GCANNNNNTGC 2 cut(s) 38, 381
BstC8I GCNNGC 3 cut(s) 39, 283, 287
BstDEI CTNAG 5 cut(s) 398, 506, 527, 696, 809
BstDSI CCRYGG 1 cut(s) 62
BstEII GGTNACC 1 cut(s) 84
BstENI CCTNNNNNAGG 1 cut(s) 297
BstF5I GGATG 2 cut(s) 555, 657
BstHHI GCGC 3 cut(s) 305, 375, 702
BstKTI GATC 1 cut(s) 505
BstMAI GTCTC 3 cut(s) 212, 232, 360
BstMBI GATC 1 cut(s) 502
BstMWI GCNNNNNNNGC 3 cut(s) 38, 370, 381
BstNI CCWGG 1 cut(s) 93
BstNSI RCATGY 2 cut(s) 199, 373
BstPI GGTNACC 1 cut(s) 84
BstSCI CCNGG 1 cut(s) 91
BstSFI CTRYAG 1 cut(s) 544
BstSLI GKGCMC 1 cut(s) 386
BstV1I GCAGC 6 cut(s) 254, 466, 488, 530, 622, 644
BsuRI GGCC 2 cut(s) 289, 464
BtgI CCRYGG 1 cut(s) 62
BtsCI GGATG 2 cut(s) 555, 657
Cac8I GCNNGC 3 cut(s) 39, 283, 287
CfoI GCGC 3 cut(s) 305, 375, 702
Cfr13I GGNCC 1 cut(s) 95
Csp6I GTAC 1 cut(s) 568
CviAII CATG 5 cut(s) 63, 190, 196, 370, 703
CviQI GTAC 1 cut(s) 568
DdeI CTNAG 5 cut(s) 398, 506, 527, 696, 809
DpnI GATC 1 cut(s) 504
DpnII GATC 1 cut(s) 502
Eam1104I CTCTTC 3 cut(s) 74, 257, 435
EarI CTCTTC 3 cut(s) 74, 257, 435
Ecl136II GAGCTC 1 cut(s) 250
Eco130I CCWWGG 2 cut(s) 62, 685
Eco24I GRGCYC 1 cut(s) 252
Eco31I GGTCTC 1 cut(s) 212
Eco47I GGWCC 1 cut(s) 95
Eco53kI GAGCTC 1 cut(s) 250
Eco88I CYCGRG 1 cut(s) 389
Eco91I GGTNACC 1 cut(s) 84
EcoICRI GAGCTC 1 cut(s) 250
EcoNI CCTNNNNNAGG 1 cut(s) 297
EcoO65I GGTNACC 1 cut(s) 84
EcoRI GAATTC 1 cut(s) 581
EcoRII CCWGG 1 cut(s) 91
EcoT14I CCWWGG 2 cut(s) 62, 685
EcoT38I GRGCYC 1 cut(s) 252
ErhI CCWWGG 2 cut(s) 62, 685
Esp3I CGTCTC 1 cut(s) 232
FaeI CATG 5 cut(s) 66, 193, 199, 373, 706
FaqI GGGAC 1 cut(s) 835
FatI CATG 5 cut(s) 62, 189, 195, 369, 702
Fnu4HI GCNGC 6 cut(s) 243, 477, 480, 544, 633, 636
FokI GGATG 2 cut(s) 562, 644
FriOI GRGCYC 1 cut(s) 252
Fsp4HI GCNGC 6 cut(s) 243, 477, 480, 544, 633, 636
FspBI CTAG 1 cut(s) 886
FspI TGCGCA 1 cut(s) 374
GlaI GCGC 3 cut(s) 304, 374, 701
GluI GCNGC 6 cut(s) 243, 477, 480, 544, 633, 636
GsuI CTGGAG 1 cut(s) 746
HaeIII GGCC 2 cut(s) 289, 464
HhaI GCGC 3 cut(s) 305, 375, 702
Hin1II CATG 5 cut(s) 66, 193, 199, 373, 706
Hin6I GCGC 3 cut(s) 303, 373, 700
HinP1I GCGC 3 cut(s) 303, 373, 700
HinfI GANTC 3 cut(s) 610, 823, 830
HphI GGTGA 1 cut(s) 194
Hpy166II GTNNAC 2 cut(s) 384, 570
Hpy188I TCNGA 2 cut(s) 119, 835
Hpy188III TCNNGA 5 cut(s) 56, 259, 296, 739, 827
Hpy8I GTNNAC 2 cut(s) 384, 570
Hpy99I CGWCG 2 cut(s) 227, 590
HpyAV CCTTC 3 cut(s) 293, 700, 781
HpyCH4V TGCA 6 cut(s) 32, 193, 384, 482, 546, 638
HpyF10VI GCNNNNNNNGC 3 cut(s) 38, 370, 381
HpyF3I CTNAG 5 cut(s) 398, 506, 527, 696, 809
Hsp92II CATG 5 cut(s) 66, 193, 199, 373, 706
HspAI GCGC 3 cut(s) 303, 373, 700
Kzo9I GATC 1 cut(s) 502
LguI GCTCTTC 1 cut(s) 257
LmnI GCTCC 2 cut(s) 676, 857
Lsp1109I GCAGC 6 cut(s) 254, 466, 488, 530, 622, 644
MaeI CTAG 1 cut(s) 886
MaeIII GTNAC 3 cut(s) 84, 182, 881
MalI GATC 1 cut(s) 504
MboI GATC 1 cut(s) 502
MboII GAAGA 5 cut(s) 88, 91, 244, 452, 754
MhlI GDGCHC 2 cut(s) 252, 386
MluCI AATT 5 cut(s) 13, 27, 415, 581, 626
MlyI GAGTC 1 cut(s) 817
MseI TTAA 2 cut(s) 468, 714
MslI CAYNNNNRTG 1 cut(s) 194
MspA1I CMGCKG 2 cut(s) 479, 635
MspR9I CCNGG 1 cut(s) 93
MvaI CCWGG 1 cut(s) 93
MwoI GCNNNNNNNGC 3 cut(s) 38, 370, 381
NcoI CCATGG 1 cut(s) 62
NdeII GATC 1 cut(s) 502
NlaIII CATG 5 cut(s) 66, 193, 199, 373, 706
NlaIV GGNNCC 1 cut(s) 60
NmeAIII GCCGAG 1 cut(s) 271
NmuCI GTSAC 1 cut(s) 182
NsbI TGCGCA 1 cut(s) 374
NspI RCATGY 2 cut(s) 199, 373
PaeR7I CTCGAG 1 cut(s) 389
PciSI GCTCTTC 1 cut(s) 257
PcsI WCGNNNNNNNCGW 1 cut(s) 498
PfeI GAWTC 2 cut(s) 610, 830
PkrI GCNGC 6 cut(s) 244, 478, 481, 545, 634, 637
PleI GAGTC 1 cut(s) 817
PpsI GAGTC 1 cut(s) 817
Psp124BI GAGCTC 1 cut(s) 252
Psp6I CCWGG 1 cut(s) 91
PspEI GGTNACC 1 cut(s) 84
PspGI CCWGG 1 cut(s) 91
PspN4I GGNNCC 1 cut(s) 60
PspPI GGNCC 1 cut(s) 95
PspXI VCTCGAGB 1 cut(s) 389
PstI CTGCAG 1 cut(s) 548
PvuII CAGCTG 2 cut(s) 479, 635
RsaI GTAC 1 cut(s) 569
RsaNI GTAC 1 cut(s) 568
RseI CAYNNNNRTG 1 cut(s) 194
SacI GAGCTC 1 cut(s) 252
SapI GCTCTTC 1 cut(s) 257
SaqAI TTAA 2 cut(s) 468, 714
SatI GCNGC 6 cut(s) 243, 477, 480, 544, 633, 636
Sau3AI GATC 1 cut(s) 502
Sau96I GGNCC 1 cut(s) 95
SchI GAGTC 1 cut(s) 817
ScrFI CCNGG 1 cut(s) 93
SduI GDGCHC 2 cut(s) 252, 386
SfcI CTRYAG 1 cut(s) 544
Sfr274I CTCGAG 1 cut(s) 389
SinI GGWCC 1 cut(s) 95
SlaI CTCGAG 1 cut(s) 389
SmiMI CAYNNNNRTG 1 cut(s) 194
SmlI CTYRAG 4 cut(s) 237, 257, 389, 825
SmoI CTYRAG 4 cut(s) 237, 257, 389, 825
Sse9I AATT 5 cut(s) 13, 27, 415, 581, 626
SsiI CCGC 1 cut(s) 533
SspMI CTAG 1 cut(s) 886
SstI GAGCTC 1 cut(s) 252
StyD4I CCNGG 1 cut(s) 91
StyI CCWWGG 2 cut(s) 62, 685
TaqI TCGA 5 cut(s) 390, 501, 585, 738, 767
TasI AATT 5 cut(s) 13, 27, 415, 581, 626
TatI WGTACW 1 cut(s) 567
TfiI GAWTC 2 cut(s) 610, 830
Tru1I TTAA 2 cut(s) 468, 714
Tru9I TTAA 2 cut(s) 468, 714
TseFI GTSAC 1 cut(s) 182
TseI GCWGC 6 cut(s) 242, 476, 479, 543, 632, 635
Tsp45I GTSAC 1 cut(s) 182
TspDTI ATGAA 2 cut(s) 719, 768
TspGWI ACGGA 1 cut(s) 587
VneI GTGCAC 1 cut(s) 382
VpaK11BI GGWCC 1 cut(s) 95
XagI CCTNNNNNAGG 1 cut(s) 297
XapI RAATTY 1 cut(s) 581
XceI RCATGY 2 cut(s) 199, 373
XhoI CTCGAG 1 cut(s) 389
XspI CTAG 1 cut(s) 886
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.