Rh6DG195700

Cytochrome p450

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6D
OG0000398
Physical Location & Seq
Forward (+)
35042628 .. 35044758
2131 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6DG195700.1

Sequence Viewer

Length: 987 bp
ATGGCTTTCATTTGGGTGATAGTTGGATTGCTTGGACTTGTTTACGTCCTGCAATCCTGGTCATGGAGAAGCAAGAAGAGGTTGCCTCCTGGTCCGAGAGGGTTTCCCATTTTCGGAAGCCTCAACTTGCTAGGGAAGTTCCCTCACAAAGATCTGCATCAACTGGCCCGAAAGTACGGTGACATCATGTACATGCGGTTAGGCCTTGTGCCTGTCATTGTCGTCTCTTCCCCTCGAGCAGCCGAGCTGTTCCTCAAAACCCACGACCTTGTGTTTGCGAGCAGGCCGCCTCACGAAGGTTCAAAGCATATTTCTTATGGCCAAAGGAACTTGACTCTTGCTGAGTATGGCTCTTACTGGCGAGACATGCGCAAGATGTGTACTCTTGAGTTACTTAGCAACCACAAAATCAACTCCTTCAAGTCAACGAGGAAAGAAGAGGTCGCCCTCATGGTGGATTCTATTCGAGAGTCTGCAGCCAAGGAACAACGTGTTTCGGTTGATCTGAGCTCCAAGGTGTCATCTCTTAGCGCAGACATGACTTGCAGGATGGTTTTTGGGAAGAAATACTCGGATGAGGAATTTAGTGAGAGGGGTTTTAAATCCGTGATTCAAGAAGGCTTGCAATTAGGAGCTGCCCCTAACTTGGGAGATTACATTCCTTGCATTGCTCCACTTGATCTCCAAGGATTCACTAAACGAATGAAGGCTGTTCACAAGGTCTTTGATGACTTTTTGGAGAAGATTGTGGAGGAGCATCTTCAATCTAGGGATCATGGAGAAAGAAGAACCAAGGACTTTGTTGATGTCATGCTGGGCTTCATGGAGTCTGAAGAATCTGCGTACCAAATCGGACGCTCAAACATCAAAGCCATTATATTGGTAAGTATATATACAATTAAGCACGTTCACATATCTGTCTTGGTATTCTTGTCTGAGTTTCATTTTATTTTCTATTTAGGTTCCTTCTATTTTTTGTTTTGCTGA

Protein Analysis

328

Amino Acids

37.59

Weight (kDa)

9.18

Isoelectric Point (pI)

44.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 29 - 293 2.7e-37 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000398)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g21960 FvH4_3g24140 FvH4_3g24150 FvH4_7g09230 FvH4_7g09260
malus_domestica MD00G1169700.v1.1 MD00G1169800.v1.1 MD01G1016400.v1.1 MD03G1188000.v1.1 MD03G1188100.v1.1 MD11G1219700.v1.1 MD11G1219900.v1.1 MD11G1220200.v1.1 MD11G1220400.v1.1 MD11G1220600.v1.1
prunus_persica Prupe.2G006500_v2.0.a1 Prupe.4G235500_v2.0.a1 Prupe.4G235600_v2.0.a1 Prupe.4G235700_v2.0.a1 Prupe.4G235700_v2.0.a1 Prupe.4G235800_v2.0.a1 Prupe.4G235900_v2.0.a1 Prupe.6G176500_v2.0.a1
pyrus_communis pycom01g04750 pycom03g14060 pycom11g19360 pycom11g19370 pycom11g19380 pycom11g19390 pycom11g19400 pycom11g19460
rosa_chinensis RchiOBHm_Chr1g0345321 RchiOBHm_Chr1g0345511 RchiOBHm_Chr1g0345781 RchiOBHm_Chr2g0115981 RchiOBHm_Chr5g0043151 RchiOBHm_Chr5g0043161 RchiOBHm_Chr5g0043901 RchiOBHm_Chr6g0274841
rosa_laevigata RLG00000013516 RLG00000018229 RLG00000028840 RLG00000028923 RLG00000034169 RLG00000034223
rosa_multiflora Rmu_co8474237.1_g000001 Rmu_sc0000698.1_g000004 Rmu_sc0000698.1_g000021 Rmu_sc0000698.1_g000023 Rmu_sc0000698.1_g000056 Rmu_sc0000698.1_g000057 Rmu_sc0000698.1_g000122 Rmu_sc0000698.1_g000123 Rmu_sc0000698.1_g000124 Rmu_sc0001292.1_g000005 Rmu_sc0001292.1_g000006 Rmu_sc0001349.1_g000002 Rmu_sc0001692.1_g000035 Rmu_sc0002079.1_g000035 Rmu_sc0002079.1_g000045 Rmu_sc0002329.1_g000017 Rmu_sc0008148.1_g000056 Rmu_sc0008148.1_g000057 Rmu_sc0008148.1_g000058 Rmu_sc0008148.1_g000062 Rmu_sc0008611.1_g000010 Rmu_sc0009227.1_g000007
rosa_roxburghii Rroxscaffold_1G00011260 Rroxscaffold_1G00037590 Rroxscaffold_2G00127610 Rroxscaffold_4G00309120 Rroxscaffold_7G00193880
rosa_rugosa Rorug01G0177400 Rorug02G0202400 Rorug05G0204600 Rorug05G0209000 Rorug06G0085100 Rorug06G0085200
rosa_samantha Rh1BG154400 Rh1DG186600 Rh2CG265200 Rh2DG267000 Rh5AG289700 Rh5AG289800 Rh5AG290000 Rh5AG293200 Rh5CG326500 Rh5DG305700 Rh5DG305800 Rh5DG310100 Rh6BG204200 Rh6CG204500 Rh6DG195700
rosa_wichuraiana Rw1G016100 Rw1G016470 Rw2G020290 Rw5G026830 Rw5G026910 Rw5G026920 Rw5G026930 Rw6G017460

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 371
AciI CCGC 2 cut(s) 196, 287
AclWI GGATC 1 cut(s) 780
AcoI YGGCCR 1 cut(s) 319
AcsI RAATTY 1 cut(s) 581
AcuI CTGAAG 1 cut(s) 852
AfaI GTAC 4 cut(s) 176, 191, 382, 845
AfiI CCNNNNNNNGG 6 cut(s) 63, 113, 296, 454, 646, 647
AflIII ACRYGT 1 cut(s) 490
AgsI TTSAA 4 cut(s) 303, 421, 614, 764
AjnI CCWGG 2 cut(s) 56, 88
AluBI AGCT 3 cut(s) 247, 510, 635
AluI AGCT 3 cut(s) 247, 510, 635
Alw21I GWGCWC 1 cut(s) 512
Alw26I GTCTC 2 cut(s) 229, 357
AlwI GGATC 1 cut(s) 780
Ama87I CYCGRG 1 cut(s) 234
AoxI GGCC 4 cut(s) 165, 202, 284, 319
ApeKI GCWGC 3 cut(s) 239, 476, 635
ApoI RAATTY 1 cut(s) 581
ArsI GACNNNNNNTTYG 2 cut(s) 257, 289
AspLEI GCGC 2 cut(s) 372, 533
AspS9I GGNCC 2 cut(s) 92, 166
AsuHPI GGTGA 2 cut(s) 28, 191
AvaI CYCGRG 1 cut(s) 234
AvaII GGWCC 1 cut(s) 92
BalI TGGCCA 1 cut(s) 321
BanII GRGCYC 1 cut(s) 512
Bbv12I GWGCWC 1 cut(s) 512
BbvI GCAGC 3 cut(s) 251, 488, 622
BccI CCATC 1 cut(s) 544
BciT130I CCWGG 2 cut(s) 58, 90
BcoDI GTCTC 2 cut(s) 229, 357
BfaI CTAG 2 cut(s) 131, 768
BfmI CTRYAG 1 cut(s) 474
BglII AGATCT 1 cut(s) 151
BisI GCNGC 4 cut(s) 240, 287, 477, 636
BlsI GCNGC 4 cut(s) 241, 288, 478, 637
Bme1390I CCNGG 2 cut(s) 58, 90
Bme18I GGWCC 1 cut(s) 92
BmeT110I CYCGRG 1 cut(s) 234
BmgT120I GGNCC 2 cut(s) 92, 166
BmiI GGNNCC 1 cut(s) 964
BmrFI CCNGG 2 cut(s) 58, 90
BmsI GCATC 2 cut(s) 166, 766
BplI GAGNNNNNCTC 4 cut(s) 70, 102, 335, 367
BpuEI CTTGAG 1 cut(s) 407
BsaBI GATNNNNATC 1 cut(s) 156
BsaJI CCNNGG 4 cut(s) 480, 513, 685, 792
BsaXI ACNNNNNCTCC 2 cut(s) 666, 696
Bsc4I CCNNNNNNNGG 6 cut(s) 63, 113, 296, 454, 646, 647
Bse1I ACTGG 2 cut(s) 168, 362
Bse3DI GCAATG 1 cut(s) 666
Bse8I GATNNNNATC 1 cut(s) 156
BseBI CCWGG 2 cut(s) 58, 90
BseDI CCNNGG 4 cut(s) 480, 513, 685, 792
BseGI GGATG 2 cut(s) 555, 580
BseJI GATNNNNATC 1 cut(s) 156
BseLI CCNNNNNNNGG 6 cut(s) 63, 113, 296, 454, 646, 647
BseMI GCAATG 1 cut(s) 666
BseMII CTCAG 3 cut(s) 333, 497, 927
BseNI ACTGG 2 cut(s) 168, 362
BseRI GAGGAG 1 cut(s) 767
BseXI GCAGC 3 cut(s) 251, 488, 622
BseYI CCCAGC 1 cut(s) 814
BshFI GGCC 4 cut(s) 167, 204, 286, 321
BsiHKAI GWGCWC 1 cut(s) 512
BsiHKCI CYCGRG 1 cut(s) 234
BslI CCNNNNNNNGG 6 cut(s) 63, 113, 296, 454, 646, 647
BsmAI GTCTC 2 cut(s) 229, 357
BsmBI CGTCTC 1 cut(s) 229
BsnI GGCC 4 cut(s) 167, 204, 286, 321
BsoBI CYCGRG 1 cut(s) 234
Bsp1286I GDGCHC 1 cut(s) 512
Bsp1407I TGTACA 1 cut(s) 189
Bsp143I GATC 4 cut(s) 151, 502, 679, 772
BspACI CCGC 2 cut(s) 196, 287
BspANI GGCC 4 cut(s) 167, 204, 286, 321
BspCNI CTCAG 3 cut(s) 334, 498, 928
BspLI GGNNCC 1 cut(s) 964
BspMAI CTGCAG 1 cut(s) 478
BspPI GGATC 1 cut(s) 780
BsrDI GCAATG 1 cut(s) 666
BsrGI TGTACA 1 cut(s) 189
BsrI ACTGG 2 cut(s) 168, 362
BssECI CCNNGG 4 cut(s) 480, 513, 685, 792
BssMI GATC 4 cut(s) 151, 502, 679, 772
BssT1I CCWWGG 4 cut(s) 480, 513, 685, 792
Bst2UI CCWGG 2 cut(s) 58, 90
Bst4CI ACNGT 1 cut(s) 179
Bst6I CTCTTC 3 cut(s) 71, 232, 432
BstAUI TGTACA 1 cut(s) 189
BstC8I GCNNGC 3 cut(s) 280, 284, 623
BstDEI CTNAG 5 cut(s) 342, 395, 506, 527, 936
BstENI CCTNNNNNAGG 1 cut(s) 294
BstF5I GGATG 2 cut(s) 555, 580
BstHHI GCGC 2 cut(s) 372, 533
BstKTI GATC 4 cut(s) 154, 505, 682, 775
BstMAI GTCTC 2 cut(s) 229, 357
BstMBI GATC 4 cut(s) 151, 502, 679, 772
BstMWI GCNNNNNNNGC 1 cut(s) 367
BstNI CCWGG 2 cut(s) 58, 90
BstNSI RCATGY 2 cut(s) 196, 370
BstSCI CCNGG 2 cut(s) 56, 88
BstSFI CTRYAG 1 cut(s) 474
BstV1I GCAGC 3 cut(s) 251, 488, 622
BstX2I RGATCY 1 cut(s) 151
BstXI CCANNNNNNTGG 1 cut(s) 880
BstYI RGATCY 1 cut(s) 151
BsuRI GGCC 4 cut(s) 167, 204, 286, 321
BtsCI GGATG 2 cut(s) 555, 580
Cac8I GCNNGC 3 cut(s) 280, 284, 623
CfoI GCGC 2 cut(s) 372, 533
Cfr13I GGNCC 2 cut(s) 92, 166
CseI GACGC 1 cut(s) 864
Csp6I GTAC 4 cut(s) 175, 190, 381, 844
CviAII CATG 9 cut(s) 63, 187, 193, 367, 451, 538, 776, 811, 823
CviQI GTAC 4 cut(s) 175, 190, 381, 844
DdeI CTNAG 5 cut(s) 342, 395, 506, 527, 936
DpnI GATC 4 cut(s) 153, 504, 681, 774
DpnII GATC 4 cut(s) 151, 502, 679, 772
DraI TTTAAA 1 cut(s) 601
EaeI YGGCCR 1 cut(s) 319
Eam1104I CTCTTC 3 cut(s) 71, 232, 432
EarI CTCTTC 3 cut(s) 71, 232, 432
Ecl136II GAGCTC 1 cut(s) 510
Eco130I CCWWGG 4 cut(s) 480, 513, 685, 792
Eco147I AGGCCT 1 cut(s) 204
Eco24I GRGCYC 1 cut(s) 512
Eco47I GGWCC 1 cut(s) 92
Eco53kI GAGCTC 1 cut(s) 510
Eco57I CTGAAG 1 cut(s) 852
Eco88I CYCGRG 1 cut(s) 234
EcoICRI GAGCTC 1 cut(s) 510
EcoNI CCTNNNNNAGG 1 cut(s) 294
EcoRII CCWGG 2 cut(s) 56, 88
EcoT14I CCWWGG 4 cut(s) 480, 513, 685, 792
EcoT38I GRGCYC 1 cut(s) 512
ErhI CCWWGG 4 cut(s) 480, 513, 685, 792
Esp3I CGTCTC 1 cut(s) 229
FaeI CATG 9 cut(s) 66, 190, 196, 370, 454, 541, 779, 814, 826
FatI CATG 9 cut(s) 62, 186, 192, 366, 450, 537, 775, 810, 822
Fnu4HI GCNGC 4 cut(s) 240, 287, 477, 636
FokI GGATG 2 cut(s) 562, 587
FriOI GRGCYC 1 cut(s) 512
Fsp4HI GCNGC 4 cut(s) 240, 287, 477, 636
FspBI CTAG 2 cut(s) 131, 768
FspI TGCGCA 1 cut(s) 371
GlaI GCGC 2 cut(s) 371, 532
GluI GCNGC 4 cut(s) 240, 287, 477, 636
GsaI CCCAGC 1 cut(s) 818
HaeIII GGCC 4 cut(s) 167, 204, 286, 321
HgaI GACGC 1 cut(s) 864
HhaI GCGC 2 cut(s) 372, 533
Hin1II CATG 9 cut(s) 66, 190, 196, 370, 454, 541, 779, 814, 826
Hin6I GCGC 2 cut(s) 370, 531
HinP1I GCGC 2 cut(s) 370, 531
HincII GTYRAC 1 cut(s) 426
HindII GTYRAC 1 cut(s) 426
HinfI GANTC 7 cut(s) 334, 458, 470, 610, 690, 827, 836
HphI GGTGA 2 cut(s) 28, 191
Hpy166II GTNNAC 5 cut(s) 43, 381, 426, 715, 910
Hpy188I TCNGA 7 cut(s) 96, 116, 507, 574, 832, 854, 937
Hpy188III TCNNGA 4 cut(s) 293, 386, 467, 614
Hpy8I GTNNAC 5 cut(s) 43, 381, 426, 715, 910
HpyAV CCTTC 5 cut(s) 290, 427, 611, 700, 976
HpyCH4III ACNGT 1 cut(s) 179
HpyCH4IV ACGT 3 cut(s) 45, 490, 906
HpyCH4V TGCA 6 cut(s) 52, 157, 476, 546, 625, 666
HpyF10VI GCNNNNNNNGC 1 cut(s) 367
HpyF3I CTNAG 5 cut(s) 342, 395, 506, 527, 936
HpySE526I ACGT 3 cut(s) 45, 490, 906
Hsp92II CATG 9 cut(s) 66, 190, 196, 370, 454, 541, 779, 814, 826
HspAI GCGC 2 cut(s) 370, 531
Kzo9I GATC 4 cut(s) 151, 502, 679, 772
LmnI GCTCC 4 cut(s) 515, 632, 676, 754
Lsp1109I GCAGC 3 cut(s) 251, 488, 622
LweI GCATC 2 cut(s) 166, 766
MaeI CTAG 2 cut(s) 131, 768
MaeII ACGT 3 cut(s) 45, 490, 906
MaeIII GTNAC 2 cut(s) 179, 390
MalI GATC 4 cut(s) 153, 504, 681, 774
MboI GATC 4 cut(s) 151, 502, 679, 772
MboII GAAGA 8 cut(s) 88, 219, 449, 574, 752, 754, 798, 845
MflI RGATCY 1 cut(s) 151
MhlI GDGCHC 1 cut(s) 512
MlsI TGGCCA 1 cut(s) 321
MluCI AATT 3 cut(s) 581, 626, 897
MluNI TGGCCA 1 cut(s) 321
MlyI GAGTC 3 cut(s) 328, 479, 836
MmeI TCCRAC 1 cut(s) 4
Mox20I TGGCCA 1 cut(s) 321
MscI TGGCCA 1 cut(s) 321
MseI TTAA 2 cut(s) 600, 900
MslI CAYNNNNRTG 2 cut(s) 14, 191
Msp20I TGGCCA 1 cut(s) 321
MspR9I CCNGG 2 cut(s) 58, 90
MvaI CCWGG 2 cut(s) 58, 90
MwoI GCNNNNNNNGC 1 cut(s) 367
NdeII GATC 4 cut(s) 151, 502, 679, 772
NlaIII CATG 9 cut(s) 66, 190, 196, 370, 454, 541, 779, 814, 826
NlaIV GGNNCC 1 cut(s) 964
NmeAIII GCCGAG 1 cut(s) 268
NmuCI GTSAC 1 cut(s) 179
NsbI TGCGCA 1 cut(s) 371
NspI RCATGY 2 cut(s) 196, 370
PaeR7I CTCGAG 1 cut(s) 234
PceI AGGCCT 1 cut(s) 204
PfeI GAWTC 4 cut(s) 458, 610, 690, 836
PkrI GCNGC 4 cut(s) 241, 288, 478, 637
PleI GAGTC 3 cut(s) 328, 478, 835
PpsI GAGTC 3 cut(s) 328, 478, 835
Psp124BI GAGCTC 1 cut(s) 512
Psp6I CCWGG 2 cut(s) 56, 88
PspFI CCCAGC 1 cut(s) 814
PspGI CCWGG 2 cut(s) 56, 88
PspN4I GGNNCC 1 cut(s) 964
PspPI GGNCC 2 cut(s) 92, 166
PspXI VCTCGAGB 1 cut(s) 234
PstI CTGCAG 1 cut(s) 478
PsuI RGATCY 1 cut(s) 151
RsaI GTAC 4 cut(s) 176, 191, 382, 845
RsaNI GTAC 4 cut(s) 175, 190, 381, 844
RseI CAYNNNNRTG 2 cut(s) 14, 191
SacI GAGCTC 1 cut(s) 512
SaqAI TTAA 2 cut(s) 600, 900
SatI GCNGC 4 cut(s) 240, 287, 477, 636
Sau3AI GATC 4 cut(s) 151, 502, 679, 772
Sau96I GGNCC 2 cut(s) 92, 166
SchI GAGTC 3 cut(s) 328, 479, 836
ScrFI CCNGG 2 cut(s) 58, 90
SduI GDGCHC 1 cut(s) 512
SfaNI GCATC 2 cut(s) 166, 766
SfcI CTRYAG 1 cut(s) 474
Sfr274I CTCGAG 1 cut(s) 234
SinI GGWCC 1 cut(s) 92
SlaI CTCGAG 1 cut(s) 234
SmiMI CAYNNNNRTG 2 cut(s) 14, 191
SmlI CTYRAG 2 cut(s) 234, 386
SmoI CTYRAG 2 cut(s) 234, 386
Sse9I AATT 3 cut(s) 581, 626, 897
SseBI AGGCCT 1 cut(s) 204
SsiI CCGC 2 cut(s) 196, 287
SspMI CTAG 2 cut(s) 131, 768
SstI GAGCTC 1 cut(s) 512
StuI AGGCCT 1 cut(s) 204
StyD4I CCNGG 2 cut(s) 56, 88
StyI CCWWGG 4 cut(s) 480, 513, 685, 792
TaaI ACNGT 1 cut(s) 179
TaiI ACGT 3 cut(s) 48, 493, 909
TaqI TCGA 2 cut(s) 235, 466
TasI AATT 3 cut(s) 581, 626, 897
TatI WGTACW 2 cut(s) 189, 380
TauI GCSGC 1 cut(s) 289
TfiI GAWTC 4 cut(s) 458, 610, 690, 836
Tru1I TTAA 2 cut(s) 600, 900
Tru9I TTAA 2 cut(s) 600, 900
TseFI GTSAC 1 cut(s) 179
TseI GCWGC 3 cut(s) 239, 476, 635
Tsp45I GTSAC 1 cut(s) 179
TspDTI ATGAA 3 cut(s) 719, 811, 932
TspGWI ACGGA 1 cut(s) 595
VpaK11BI GGWCC 1 cut(s) 92
XagI CCTNNNNNAGG 1 cut(s) 294
XapI RAATTY 1 cut(s) 581
XceI RCATGY 2 cut(s) 196, 370
XhoI CTCGAG 1 cut(s) 234
XspI CTAG 2 cut(s) 131, 768
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.