FvH4_4g16620

strictosidine

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0000674
Physical Location & Seq
Forward (+)
20493475 .. 20494732
1258 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g16620.t1

Sequence Viewer

Length: 696 bp
ATGTTGATGGACAACTCTTACTTTTGTGGCTTTTATGATAGCTTCTGCGGGCAAAGGATATGTGATGGTTCAACCGACAATAAGACTGAACCAATATGTGGAAGGCCTCTAGGTTTGAAGCTCAACCCAAAAACTTGTGAACTCTATATTGCAGATGCCTACTTCGGTCTCTTAAAGATAGGTCGCAACGGTGGTCATCTTCAACGACTTGCCACTTCCCTGAATGGAGTCCCTTTCCGTTTTCTGAATGCTCTGGACATTGATACTCAAACTGGAATTCTTTATTTTACAGACACCAGCACAGTTTACCAAAGAAGGTTGTGGCCTTTGTCATTCGTAACCGGTGACAAAACCGGAAGGTTATTGCATTACGATCCTCGTAGAAAGAAGGTGTCTGTGCTGCTCAATAACTTGGCTTTTGCAGACGGTGTGGCTCTAAGCAAGGACAGATCTTTTCTCCTAGTAGCCGAAACTGCTACGTTCAAAATCTTCAAGTTTTGGCTTCGAGGACCCAAAGCTGGTTTGTGGTTGACGAAAGATCCGGTGGGAGTAAGGTTTGATGAAGAAGGAAACGTTGTGGAGGTGTTGGATGGAGAGGGTGGTCCGACGCTTGAGTCTGTTACTGAGGTTAACGAACACAATGGGAAGCTGTGGATTGGATCGTCAGCAAAGCCCTATGTTGGTGTTATCAATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Str_synth

Protein Analysis

232

Amino Acids

25.71

Weight (kDa)

8.22

Isoelectric Point (pI)

30.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Str_synth PF03088 83 - 170 8.1e-29 Strictosidine synthase
SGL PF08450 114 - 171 9.7e-06 SMP-30/Gluconolactonase/LRE-like region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 613
AccB7I CCANNNNNTGG 1 cut(s) 98
AciI CCGC 1 cut(s) 48
AclI AACGTT 1 cut(s) 573
AclWI GGATC 3 cut(s) 368, 533, 667
AcsI RAATTY 1 cut(s) 276
AfiI CCNNNNNNNGG 3 cut(s) 98, 518, 680
AgeI ACCGGT 1 cut(s) 341
AgsI TTSAA 5 cut(s) 72, 118, 203, 484, 493
AluBI AGCT 4 cut(s) 42, 121, 518, 649
AluI AGCT 4 cut(s) 42, 121, 518, 649
Alw26I GTCTC 1 cut(s) 173
AlwI GGATC 3 cut(s) 368, 533, 667
AoxI GGCC 2 cut(s) 104, 323
ApeKI GCWGC 1 cut(s) 400
ApoI RAATTY 1 cut(s) 276
AsiGI ACCGGT 1 cut(s) 341
AspS9I GGNCC 2 cut(s) 509, 602
AsuHPI GGTGA 1 cut(s) 356
AvaII GGWCC 2 cut(s) 509, 602
BbvI GCAGC 1 cut(s) 387
BccI CCATC 2 cut(s) 59, 584
BcoDI GTCTC 1 cut(s) 173
BfaI CTAG 2 cut(s) 110, 461
BglII AGATCT 1 cut(s) 449
BisI GCNGC 1 cut(s) 401
BlsI GCNGC 1 cut(s) 402
Bme18I GGWCC 2 cut(s) 509, 602
BmgT120I GGNCC 2 cut(s) 509, 602
BmiI GGNNCC 1 cut(s) 511
BmsI GCATC 1 cut(s) 145
BpuEI CTTGAG 1 cut(s) 632
BsaI GGTCTC 1 cut(s) 173
BsaWI WCCGGW 3 cut(s) 341, 353, 541
Bsc4I CCNNNNNNNGG 3 cut(s) 98, 518, 680
Bse118I RCCGGY 1 cut(s) 341
Bse1I ACTGG 1 cut(s) 277
BseGI GGATG 1 cut(s) 595
BseLI CCNNNNNNNGG 3 cut(s) 98, 518, 680
BseMII CTCAG 1 cut(s) 615
BseNI ACTGG 1 cut(s) 277
BseXI GCAGC 1 cut(s) 387
BshFI GGCC 2 cut(s) 106, 325
BshTI ACCGGT 1 cut(s) 341
BsiSI CCGG 3 cut(s) 342, 354, 542
BslFI GGGAC 1 cut(s) 215
BslI CCNNNNNNNGG 3 cut(s) 98, 518, 680
BsmAI GTCTC 1 cut(s) 173
BsmFI GGGAC 1 cut(s) 215
BsmI GAATGC 1 cut(s) 253
BsnI GGCC 2 cut(s) 106, 325
Bso31I GGTCTC 1 cut(s) 173
Bsp143I GATC 4 cut(s) 373, 449, 538, 659
BspACI CCGC 1 cut(s) 48
BspANI GGCC 2 cut(s) 106, 325
BspCNI CTCAG 1 cut(s) 616
BspLI GGNNCC 1 cut(s) 511
BspPI GGATC 3 cut(s) 368, 533, 667
BspTNI GGTCTC 1 cut(s) 173
BsrFI RCCGGY 1 cut(s) 341
BsrI ACTGG 1 cut(s) 277
BssAI RCCGGY 1 cut(s) 341
BssMI GATC 4 cut(s) 373, 449, 538, 659
Bst4CI ACNGT 3 cut(s) 191, 304, 428
BstC8I GCNNGC 1 cut(s) 50
BstDEI CTNAG 2 cut(s) 437, 624
BstF5I GGATG 1 cut(s) 595
BstKTI GATC 4 cut(s) 376, 452, 541, 662
BstMAI GTCTC 1 cut(s) 173
BstMBI GATC 4 cut(s) 373, 449, 538, 659
BstMWI GCNNNNNNNGC 1 cut(s) 473
BstV1I GCAGC 1 cut(s) 387
BstX2I RGATCY 2 cut(s) 449, 538
BstYI RGATCY 2 cut(s) 449, 538
BsuRI GGCC 2 cut(s) 106, 325
BtsCI GGATG 1 cut(s) 595
Cac8I GCNNGC 1 cut(s) 50
Cfr10I RCCGGY 1 cut(s) 341
Cfr13I GGNCC 2 cut(s) 509, 602
CseI GACGC 1 cut(s) 616
CspAI ACCGGT 1 cut(s) 341
DdeI CTNAG 2 cut(s) 437, 624
DpnI GATC 4 cut(s) 375, 451, 540, 661
DpnII GATC 4 cut(s) 373, 449, 538, 659
DrdI GACNNNNNNGTC 1 cut(s) 613
DseDI GACNNNNNNGTC 1 cut(s) 613
Eco147I AGGCCT 1 cut(s) 106
Eco31I GGTCTC 1 cut(s) 173
Eco47I GGWCC 2 cut(s) 509, 602
EcoO109I RGGNCCY 1 cut(s) 509
EcoRI GAATTC 1 cut(s) 276
FaiI YATR 5 cut(s) 36, 61, 97, 147, 678
FaqI GGGAC 1 cut(s) 215
FauI CCCGC 1 cut(s) 41
Fnu4HI GCNGC 1 cut(s) 401
FokI GGATG 1 cut(s) 602
Fsp4HI GCNGC 1 cut(s) 401
FspBI CTAG 2 cut(s) 110, 461
GluI GCNGC 1 cut(s) 401
HaeIII GGCC 2 cut(s) 106, 325
HapII CCGG 3 cut(s) 342, 354, 542
HgaI GACGC 1 cut(s) 616
HincII GTYRAC 2 cut(s) 531, 631
HindII GTYRAC 2 cut(s) 531, 631
HinfI GANTC 2 cut(s) 228, 614
HpaI GTTAAC 1 cut(s) 631
HpaII CCGG 3 cut(s) 342, 354, 542
HphI GGTGA 1 cut(s) 356
Hpy166II GTNNAC 4 cut(s) 140, 307, 531, 631
Hpy188I TCNGA 2 cut(s) 246, 606
Hpy188III TCNNGA 1 cut(s) 254
Hpy8I GTNNAC 4 cut(s) 140, 307, 531, 631
Hpy99I CGWCG 1 cut(s) 610
HpyAV CCTTC 5 cut(s) 96, 309, 351, 382, 560
HpyCH4III ACNGT 3 cut(s) 191, 304, 428
HpyCH4IV ACGT 2 cut(s) 479, 573
HpyCH4V TGCA 3 cut(s) 152, 367, 422
HpyF10VI GCNNNNNNNGC 1 cut(s) 473
HpyF3I CTNAG 2 cut(s) 437, 624
HpySE526I ACGT 2 cut(s) 479, 573
KspAI GTTAAC 1 cut(s) 631
Kzo9I GATC 4 cut(s) 373, 449, 538, 659
LpnPI CCDG 8 cut(s) 233, 239, 258, 310, 355, 367, 504, 555
Lsp1109I GCAGC 1 cut(s) 387
LweI GCATC 1 cut(s) 145
MaeI CTAG 2 cut(s) 110, 461
MaeII ACGT 2 cut(s) 479, 573
MaeIII GTNAC 3 cut(s) 337, 344, 619
MalI GATC 4 cut(s) 375, 451, 540, 661
MboI GATC 4 cut(s) 373, 449, 538, 659
MboII GAAGA 3 cut(s) 191, 481, 575
MflI RGATCY 2 cut(s) 449, 538
MluCI AATT 2 cut(s) 276, 691
MlyI GAGTC 2 cut(s) 237, 623
MmeI TCCRAC 2 cut(s) 567, 629
MnlI CCTC 6 cut(s) 117, 387, 500, 574, 589, 619
MseI TTAA 2 cut(s) 173, 630
MspI CCGG 3 cut(s) 342, 354, 542
Mva1269I GAATGC 1 cut(s) 253
MwoI GCNNNNNNNGC 1 cut(s) 473
NdeII GATC 4 cut(s) 373, 449, 538, 659
NlaIV GGNNCC 1 cut(s) 511
NmuCI GTSAC 1 cut(s) 344
PceI AGGCCT 1 cut(s) 106
PctI GAATGC 1 cut(s) 253
PflMI CCANNNNNTGG 1 cut(s) 98
PinAI ACCGGT 1 cut(s) 341
PkrI GCNGC 1 cut(s) 402
PleI GAGTC 2 cut(s) 236, 622
PpsI GAGTC 2 cut(s) 236, 622
PpuMI RGGWCCY 1 cut(s) 509
Psp1406I AACGTT 1 cut(s) 573
Psp5II RGGWCCY 1 cut(s) 509
PspN4I GGNNCC 1 cut(s) 511
PspPI GGNCC 2 cut(s) 509, 602
PspPPI RGGWCCY 1 cut(s) 509
PsuI RGATCY 2 cut(s) 449, 538
SaqAI TTAA 2 cut(s) 173, 630
SatI GCNGC 1 cut(s) 401
Sau3AI GATC 4 cut(s) 373, 449, 538, 659
Sau96I GGNCC 2 cut(s) 509, 602
SchI GAGTC 2 cut(s) 237, 623
SfaNI GCATC 1 cut(s) 145
SinI GGWCC 2 cut(s) 509, 602
SmlI CTYRAG 1 cut(s) 611
SmoI CTYRAG 1 cut(s) 611
Sse9I AATT 2 cut(s) 276, 691
SseBI AGGCCT 1 cut(s) 106
SsiI CCGC 1 cut(s) 48
SspMI CTAG 2 cut(s) 110, 461
StuI AGGCCT 1 cut(s) 106
TaaI ACNGT 3 cut(s) 191, 304, 428
TaiI ACGT 2 cut(s) 482, 576
TaqI TCGA 1 cut(s) 505
TaqII GACCGA 1 cut(s) 155
TasI AATT 2 cut(s) 276, 691
Tru1I TTAA 2 cut(s) 173, 630
Tru9I TTAA 2 cut(s) 173, 630
TseFI GTSAC 1 cut(s) 344
TseI GCWGC 1 cut(s) 400
Tsp45I GTSAC 1 cut(s) 344
TspDTI ATGAA 1 cut(s) 576
TspGWI ACGGA 1 cut(s) 227
Van91I CCANNNNNTGG 1 cut(s) 98
VpaK11BI GGWCC 2 cut(s) 509, 602
XapI RAATTY 1 cut(s) 276
XspI CTAG 2 cut(s) 110, 461
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.