FvH4_7g06670

strictosidine

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0000674
Physical Location & Seq
Reverse (-)
6710455 .. 6711661
1207 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g06670.t1

Sequence Viewer

Length: 966 bp
ATGAAGAAGTCAGCTCTTGTTATTGTCTTCTTCTTTTTCTTTTTGTCTAGTAACCAAGCCCATGCAGCATATTTTTCACTCCCAAACCTAAACCACCATGAAATACCCATCCATGTACCACAACCCGAAAGCATTGCTTTCGACTGCAATGGCGCACCCTACGTGGGTGTGGCTGATGGCAGGATATTCAAGTGGCTGGGACGCCCATTTGGGTGGACAGAATTCACAGTCACCGCACCCAATAGGCCGAGAGAAGTATGTGATCTCTCAACGAGCAAAGGCAGCGAACCGATATGCGGGAGGCCACTTGGTTTAAAATTTGACCTCAACACATGCGAACTGTATATTGCAGATGCCTATTTCGGGCTTCTAAAGACAGGTCCAAATGGTGGAGTAGCTCAAACACTTGCAACATCTGCTGAAGGATTCCCTTTCCGTTTCTTAAATGGTGTGGACGTTGATCAAGAAACTGGAATGGTTTACTTTACAGACACCAGTGCTATTTATCAAAGAAGGAACTGGGAATTGTCAGTCAGCACAGGTGATAAAACTGGAAGGTTAATGCTATATGATCCATCGACAAAGAATGTGACGGTGTTGCTTCATGGCTTGGCTTTTGCAAACGGGGTGGCACTAAGTAAGGATGGTGATTTTGTTCTAGTGACCCAAACCACTGCAAAGAACATTTTGAGGTATTGGCTTCGAGGGCCTAGAGCCAACACTGTTGATACTTTTTTCAACCTCCAAGGAGCCCCAGACAACATCAACAGAAATCTAAATGGAGAATTTTGGGTTGCTGAAAACAGTGCTGGAAGAGGTAAAGCGGTGAAGCTTGATGCCCAAGGAAATTTTCTGGATGTTGTAGATGGACTCAACCCTGTGAGTCATGTTGAGCAGCGTCATGGAAACCTTTGGATAGGATCTGTTGTTAATAACTTTGTTGGGGTCATTCTTAATTTTGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Str_synth

Protein Analysis

322

Amino Acids

35.35

Weight (kDa)

6.38

Isoelectric Point (pI)

20.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Str_synth PF03088 149 - 236 1.5e-31 Strictosidine synthase
SGL PF08450 180 - 289 2.5e-09 SMP-30/Gluconolactonase/LRE-like region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 389
AciI CCGC 3 cut(s) 234, 297, 824
AclWI GGATC 2 cut(s) 566, 928
AcsI RAATTY 4 cut(s) 221, 317, 785, 847
AcuI CTGAAG 1 cut(s) 441
AcyI GRCGYC 1 cut(s) 202
AfaI GTAC 1 cut(s) 117
AfiI CCNNNNNNNGG 4 cut(s) 164, 296, 363, 389
AgsI TTSAA 2 cut(s) 190, 739
AjuI GAANNNNNNNTTGG 2 cut(s) 834, 866
AluBI AGCT 3 cut(s) 14, 398, 832
AluI AGCT 3 cut(s) 14, 398, 832
AlwI GGATC 2 cut(s) 566, 928
AoxI GGCC 3 cut(s) 245, 302, 707
ApeKI GCWGC 3 cut(s) 65, 282, 895
ApoI RAATTY 4 cut(s) 221, 317, 785, 847
AspLEI GCGC 1 cut(s) 155
AspS9I GGNCC 2 cut(s) 380, 707
AsuHPI GGTGA 4 cut(s) 223, 554, 659, 838
AvaII GGWCC 1 cut(s) 380
BanII GRGCYC 1 cut(s) 754
BbsI GAAGAC 1 cut(s) 19
BbvI GCAGC 3 cut(s) 77, 294, 907
BccI CCATC 5 cut(s) 116, 170, 583, 638, 860
BcgI CGANNNNNNTGC 4 cut(s) 116, 121, 150, 155
BclI TGATCA 1 cut(s) 460
BfaI CTAG 3 cut(s) 48, 659, 711
BisI GCNGC 3 cut(s) 66, 283, 896
BlsI GCNGC 3 cut(s) 67, 284, 897
Bme18I GGWCC 1 cut(s) 380
BmgT120I GGNCC 2 cut(s) 380, 707
BmiI GGNNCC 1 cut(s) 751
BmrI ACTGGG 1 cut(s) 529
BmsI GCATC 2 cut(s) 343, 826
BmuI ACTGGG 1 cut(s) 529
BpiI GAAGAC 1 cut(s) 19
BsaAI YACGTR 1 cut(s) 163
BsaHI GRCGYC 1 cut(s) 202
BsaJI CCNNGG 2 cut(s) 745, 841
Bsc4I CCNNNNNNNGG 4 cut(s) 164, 296, 363, 389
Bse1I ACTGG 4 cut(s) 475, 495, 524, 556
Bse3DI GCAATG 2 cut(s) 132, 154
BseDI CCNNGG 2 cut(s) 745, 841
BseGI GGATG 3 cut(s) 108, 649, 862
BseLI CCNNNNNNNGG 4 cut(s) 164, 296, 363, 389
BseMI GCAATG 2 cut(s) 132, 154
BseNI ACTGG 4 cut(s) 475, 495, 524, 556
BseXI GCAGC 3 cut(s) 77, 294, 907
BseYI CCCAGC 1 cut(s) 196
BshFI GGCC 3 cut(s) 247, 304, 709
BslFI GGGAC 1 cut(s) 213
BslI CCNNNNNNNGG 4 cut(s) 164, 296, 363, 389
BsmFI GGGAC 1 cut(s) 213
BsnI GGCC 3 cut(s) 247, 304, 709
Bsp1286I GDGCHC 1 cut(s) 754
Bsp143I GATC 4 cut(s) 262, 460, 571, 920
BspACI CCGC 3 cut(s) 234, 297, 824
BspANI GGCC 3 cut(s) 247, 304, 709
BspLI GGNNCC 1 cut(s) 751
BspPI GGATC 2 cut(s) 566, 928
BsrDI GCAATG 2 cut(s) 132, 154
BsrI ACTGG 4 cut(s) 475, 495, 524, 556
BssECI CCNNGG 2 cut(s) 745, 841
BssMI GATC 4 cut(s) 262, 460, 571, 920
BssNI GRCGYC 1 cut(s) 202
BssT1I CCWWGG 2 cut(s) 745, 841
Bst4CI ACNGT 5 cut(s) 229, 342, 595, 724, 806
Bst6I CTCTTC 1 cut(s) 808
BstACI GRCGYC 1 cut(s) 202
BstAPI GCANNNNNTGC 1 cut(s) 416
BstBAI YACGTR 1 cut(s) 163
BstDEI CTNAG 1 cut(s) 635
BstF5I GGATG 3 cut(s) 108, 649, 862
BstHHI GCGC 1 cut(s) 155
BstKTI GATC 4 cut(s) 265, 463, 574, 923
BstMBI GATC 4 cut(s) 262, 460, 571, 920
BstMWI GCNNNNNNNGC 4 cut(s) 65, 282, 416, 706
BstNSI RCATGY 1 cut(s) 336
BstV1I GCAGC 3 cut(s) 77, 294, 907
BstV2I GAAGAC 1 cut(s) 19
BstX2I RGATCY 1 cut(s) 920
BstXI CCANNNNNNTGG 1 cut(s) 213
BstYI RGATCY 1 cut(s) 920
BsuRI GGCC 3 cut(s) 247, 304, 709
BtsCI GGATG 3 cut(s) 108, 649, 862
BtsI GCAGTG 1 cut(s) 672
BtsIMutI CAGTG 4 cut(s) 502, 672, 720, 811
CfoI GCGC 1 cut(s) 155
Cfr13I GGNCC 2 cut(s) 380, 707
CseI GACGC 2 cut(s) 210, 887
Csp6I GTAC 1 cut(s) 116
CspCI CAANNNNNGTGG 2 cut(s) 609, 644
CviAII CATG 7 cut(s) 62, 98, 113, 333, 605, 887, 902
CviQI GTAC 1 cut(s) 116
DdeI CTNAG 1 cut(s) 635
DpnI GATC 4 cut(s) 264, 462, 573, 922
DpnII GATC 4 cut(s) 262, 460, 571, 920
DraI TTTAAA 1 cut(s) 315
Eam1104I CTCTTC 1 cut(s) 808
EarI CTCTTC 1 cut(s) 808
Eco130I CCWWGG 2 cut(s) 745, 841
Eco24I GRGCYC 1 cut(s) 754
Eco47I GGWCC 1 cut(s) 380
Eco57I CTGAAG 1 cut(s) 441
EcoO109I RGGNCCY 1 cut(s) 707
EcoRI GAATTC 1 cut(s) 221
EcoT14I CCWWGG 2 cut(s) 745, 841
EcoT38I GRGCYC 1 cut(s) 754
ErhI CCWWGG 2 cut(s) 745, 841
FaeI CATG 7 cut(s) 65, 101, 116, 336, 608, 890, 905
FalI AAGNNNNNCTT 2 cut(s) 121, 153
FaqI GGGAC 1 cut(s) 213
FatI CATG 7 cut(s) 61, 97, 112, 332, 604, 886, 901
FauI CCCGC 1 cut(s) 290
FbaI TGATCA 1 cut(s) 460
Fnu4HI GCNGC 3 cut(s) 66, 283, 896
FokI GGATG 3 cut(s) 95, 656, 869
FriOI GRGCYC 1 cut(s) 754
Fsp4HI GCNGC 3 cut(s) 66, 283, 896
FspBI CTAG 3 cut(s) 48, 659, 711
GlaI GCGC 1 cut(s) 154
GluI GCNGC 3 cut(s) 66, 283, 896
GsaI CCCAGC 1 cut(s) 200
HaeIII GGCC 3 cut(s) 247, 304, 709
HgaI GACGC 2 cut(s) 210, 887
HhaI GCGC 1 cut(s) 155
Hin1I GRCGYC 1 cut(s) 202
Hin1II CATG 7 cut(s) 65, 101, 116, 336, 608, 890, 905
Hin6I GCGC 1 cut(s) 153
HinP1I GCGC 1 cut(s) 153
HindIII AAGCTT 1 cut(s) 830
HinfI GANTC 3 cut(s) 426, 870, 883
HphI GGTGA 4 cut(s) 223, 554, 659, 838
Hpy166II GTNNAC 3 cut(s) 216, 454, 481
Hpy188III TCNNGA 2 cut(s) 464, 854
Hpy8I GTNNAC 3 cut(s) 216, 454, 481
HpyAV CCTTC 3 cut(s) 416, 507, 549
HpyCH4III ACNGT 5 cut(s) 229, 342, 595, 724, 806
HpyCH4IV ACGT 2 cut(s) 162, 456
HpyCH4V TGCA 6 cut(s) 65, 147, 350, 410, 620, 677
HpyF10VI GCNNNNNNNGC 4 cut(s) 65, 282, 416, 706
HpyF3I CTNAG 1 cut(s) 635
HpySE526I ACGT 2 cut(s) 162, 456
Hsp92I GRCGYC 1 cut(s) 202
Hsp92II CATG 7 cut(s) 65, 101, 116, 336, 608, 890, 905
HspAI GCGC 1 cut(s) 153
Ksp22I TGATCA 1 cut(s) 460
Kzo9I GATC 4 cut(s) 262, 460, 571, 920
LmnI GCTCC 1 cut(s) 749
Lsp1109I GCAGC 3 cut(s) 77, 294, 907
LweI GCATC 2 cut(s) 343, 826
MaeI CTAG 3 cut(s) 48, 659, 711
MaeII ACGT 2 cut(s) 162, 456
MaeIII GTNAC 4 cut(s) 50, 229, 589, 661
MalI GATC 4 cut(s) 264, 462, 573, 922
MboI GATC 4 cut(s) 262, 460, 571, 920
MboII GAAGA 4 cut(s) 16, 19, 22, 825
MflI RGATCY 1 cut(s) 920
MhlI GDGCHC 1 cut(s) 754
MluCI AATT 6 cut(s) 221, 317, 524, 785, 847, 955
MlyI GAGTC 2 cut(s) 864, 892
MnlI CCTC 6 cut(s) 294, 335, 684, 698, 752, 809
MseI TTAA 5 cut(s) 314, 443, 560, 930, 954
MslI CAYNNNNRTG 1 cut(s) 211
MwoI GCNNNNNNNGC 4 cut(s) 65, 282, 416, 706
NdeII GATC 4 cut(s) 262, 460, 571, 920
NlaIII CATG 7 cut(s) 65, 101, 116, 336, 608, 890, 905
NlaIV GGNNCC 1 cut(s) 751
NmeAIII GCCGAG 1 cut(s) 273
NmuCI GTSAC 3 cut(s) 229, 589, 661
NspI RCATGY 1 cut(s) 336
PfeI GAWTC 1 cut(s) 426
PflMI CCANNNNNTGG 1 cut(s) 389
PkrI GCNGC 3 cut(s) 67, 284, 897
PleI GAGTC 2 cut(s) 864, 891
PpsI GAGTC 2 cut(s) 864, 891
Ppu21I YACGTR 1 cut(s) 163
PspFI CCCAGC 1 cut(s) 196
PspN4I GGNNCC 1 cut(s) 751
PspPI GGNCC 2 cut(s) 380, 707
PsuI RGATCY 1 cut(s) 920
RsaI GTAC 1 cut(s) 117
RsaNI GTAC 1 cut(s) 116
RseI CAYNNNNRTG 1 cut(s) 211
SaqAI TTAA 5 cut(s) 314, 443, 560, 930, 954
SatI GCNGC 3 cut(s) 66, 283, 896
Sau3AI GATC 4 cut(s) 262, 460, 571, 920
Sau96I GGNCC 2 cut(s) 380, 707
SchI GAGTC 2 cut(s) 864, 892
SduI GDGCHC 1 cut(s) 754
SfaNI GCATC 2 cut(s) 343, 826
SinI GGWCC 1 cut(s) 380
SmiMI CAYNNNNRTG 1 cut(s) 211
Sse9I AATT 6 cut(s) 221, 317, 524, 785, 847, 955
SsiI CCGC 3 cut(s) 234, 297, 824
SspMI CTAG 3 cut(s) 48, 659, 711
StyI CCWWGG 2 cut(s) 745, 841
TaaI ACNGT 5 cut(s) 229, 342, 595, 724, 806
TaiI ACGT 2 cut(s) 165, 459
TaqI TCGA 3 cut(s) 141, 578, 703
TasI AATT 6 cut(s) 221, 317, 524, 785, 847, 955
TfiI GAWTC 1 cut(s) 426
Tru1I TTAA 5 cut(s) 314, 443, 560, 930, 954
Tru9I TTAA 5 cut(s) 314, 443, 560, 930, 954
TscAI CASTG 4 cut(s) 502, 679, 727, 811
TseFI GTSAC 3 cut(s) 229, 589, 661
TseI GCWGC 3 cut(s) 65, 282, 895
Tsp45I GTSAC 3 cut(s) 229, 589, 661
TspDTI ATGAA 3 cut(s) 17, 114, 593
TspGWI ACGGA 1 cut(s) 425
TspRI CASTG 4 cut(s) 502, 679, 727, 811
Van91I CCANNNNNTGG 1 cut(s) 389
VpaK11BI GGWCC 1 cut(s) 380
XapI RAATTY 4 cut(s) 221, 317, 785, 847
XceI RCATGY 1 cut(s) 336
XspI CTAG 3 cut(s) 48, 659, 711
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.