Rh4BG358600

strictosidine

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4B
OG0000674
Physical Location & Seq
Reverse (-)
51846462 .. 51849031
2570 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4BG358600.1

Sequence Viewer

Length: 1056 bp
ATGAAGCTACATTTTCTTTCACTCTTGTTCTTCTTTTCGTTAATCCTAGCATCATCCACACCAGCATCAGCTTTCTTTGTTGTCAACCTCACAAATTACCATCAGATCGAGCTCCCAAAAAGCGTAGTTGGTCCGGAAAGCATTGCGTTCGACTGCCGTGGAAAAGGACCTTATGTTGGAGTGTCAGATGGAAGAATTCTCAAATGGCAAGGGCCTTGCCGGGGCTGGACGGAGTTTGCCTTTACCTCCCCAACCAGGCCAAGGAAAATATGTGATGGTTCAACCGATAATACGACTGAACCAATATGTGGAAGGCCACTAGGCTTGAAGTTCAACCCAAAAACTTGTGAACTCTATATAGCAGATGCCTACTTCGGGCTGTTAAAGATAGGTCGCAATGGTGGTCGTCCTCAACAACTTGCCACTTCCCTAAATGGAGTCCCTTTCCGTTTTCTGAATGCTCTGGACATTGATTGTCAAAGTGGAATTGTTTATTTTACAGACACCAGCACAGTTTACCAAAGAAGGTTGTGGCCTTTGTCAATTGCCACCGGTGACAAAACCGGAAGGTTATTGCAATACGACCCTTGTACAAAGAAGGTGACTGTGTTGCTCGATAACTTGGCTTTTGCAAATGGTGTGGCTCTAAGCAAGGACAGTTCTTTCCTCCTAGTAGCCGAATCCGCCACATTCAAAATCTTCAAGCTTTGGCTTCGAGGACCCAAAGCTCACGACGTAGAACTCTTCTCTCAACTGAAAAGGCCAGCCGATAACATTAAGAGGACCAACAGTGGTGAATTTTGGGCTGCACTCAATAGTCTAAGAGGATTACAGGGTAACCAGACTTCTCCATCCTTGTGGTTGACGAAAGATCCGGTGGGAGTGAAGTTTGATGAACAAGGAAACGTAATGGAGGTGTTAGATGGAGAGGGTGGTCCGACGCTTGAGTCCATTAGTGAGGTTGAAGAACACAACGGAAAGTTGTGGATTGGATCGGTAGTGAAGTCCTATGTTGGAGTTATCAAGTCATACATAATATATCCACATGCACTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Str_synth

Protein Analysis

351

Amino Acids

38.8

Weight (kDa)

8.85

Isoelectric Point (pI)

31.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Str_synth PF03088 153 - 240 5.3e-28 Strictosidine synthase
SGL PF08450 184 - 273 1.3e-07 SMP-30/Gluconolactonase/LRE-like region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 946
AccB7I CCANNNNNTGG 1 cut(s) 308
AccIII TCCGGA 1 cut(s) 133
AciI CCGC 1 cut(s) 684
AclWI GGATC 2 cut(s) 866, 1000
AcsI RAATTY 2 cut(s) 195, 797
AfaI GTAC 1 cut(s) 592
AfiI CCNNNNNNNGG 6 cut(s) 176, 221, 255, 261, 308, 375
AgeI ACCGGT 1 cut(s) 551
AgsI TTSAA 6 cut(s) 282, 328, 334, 694, 703, 965
AjnI CCWGG 1 cut(s) 254
AluBI AGCT 5 cut(s) 7, 71, 112, 706, 728
AluI AGCT 5 cut(s) 7, 71, 112, 706, 728
Alw21I GWGCWC 1 cut(s) 114
AlwI GGATC 2 cut(s) 866, 1000
Aor13HI TCCGGA 1 cut(s) 133
AoxI GGCC 5 cut(s) 212, 257, 314, 533, 761
ApeKI GCWGC 1 cut(s) 806
ApoI RAATTY 2 cut(s) 195, 797
AsiGI ACCGGT 1 cut(s) 551
AspS9I GGNCC 6 cut(s) 131, 167, 212, 719, 783, 935
AsuC2I CCSGG 1 cut(s) 221
AsuHPI GGTGA 3 cut(s) 566, 613, 806
AvaII GGWCC 5 cut(s) 131, 167, 719, 783, 935
BanII GRGCYC 1 cut(s) 114
BarI GAAGNNNNNNTAC 2 cut(s) 829, 861
Bbv12I GWGCWC 1 cut(s) 114
BbvI GCAGC 1 cut(s) 793
BccI CCATC 5 cut(s) 108, 182, 269, 859, 917
BceAI ACGGC 1 cut(s) 141
BcgI CGANNNNNNTGC 2 cut(s) 130, 164
BciT130I CCWGG 1 cut(s) 256
BcnI CCSGG 1 cut(s) 221
BfaI CTAG 3 cut(s) 47, 320, 671
BisI GCNGC 1 cut(s) 807
BlsI GCNGC 1 cut(s) 808
Bme1390I CCNGG 2 cut(s) 221, 256
Bme18I GGWCC 5 cut(s) 131, 167, 719, 783, 935
BmgT120I GGNCC 6 cut(s) 131, 167, 212, 719, 783, 935
BmiI GGNNCC 1 cut(s) 721
BmrFI CCNGG 2 cut(s) 221, 256
BmsI GCATC 3 cut(s) 59, 74, 355
BpuEI CTTGAG 1 cut(s) 965
BpuMI CCSGG 1 cut(s) 221
BsaJI CCNNGG 3 cut(s) 157, 220, 260
BsaWI WCCGGW 4 cut(s) 133, 551, 563, 874
Bsc4I CCNNNNNNNGG 6 cut(s) 176, 221, 255, 261, 308, 375
Bse118I RCCGGY 1 cut(s) 551
Bse3DI GCAATG 2 cut(s) 141, 403
BseAI TCCGGA 1 cut(s) 133
BseBI CCWGG 1 cut(s) 256
BseDI CCNNGG 3 cut(s) 157, 220, 260
BseGI GGATG 2 cut(s) 53, 851
BseLI CCNNNNNNNGG 6 cut(s) 176, 221, 255, 261, 308, 375
BseMI GCAATG 2 cut(s) 141, 403
BseXI GCAGC 1 cut(s) 793
BsgI GTGCAG 1 cut(s) 792
BshFI GGCC 5 cut(s) 214, 259, 316, 535, 763
BshTI ACCGGT 1 cut(s) 551
BsiHKAI GWGCWC 1 cut(s) 114
BsiSI CCGG 5 cut(s) 134, 220, 552, 564, 875
BslFI GGGAC 1 cut(s) 425
BslI CCNNNNNNNGG 6 cut(s) 176, 221, 255, 261, 308, 375
BsmFI GGGAC 1 cut(s) 425
BsmI GAATGC 1 cut(s) 463
BsnI GGCC 5 cut(s) 214, 259, 316, 535, 763
Bsp1286I GDGCHC 1 cut(s) 114
Bsp13I TCCGGA 1 cut(s) 133
Bsp1407I TGTACA 1 cut(s) 590
Bsp143I GATC 3 cut(s) 105, 871, 992
BspACI CCGC 1 cut(s) 684
BspANI GGCC 5 cut(s) 214, 259, 316, 535, 763
BspEI TCCGGA 1 cut(s) 133
BspLI GGNNCC 1 cut(s) 721
BspPI GGATC 2 cut(s) 866, 1000
BsrDI GCAATG 2 cut(s) 141, 403
BsrFI RCCGGY 1 cut(s) 551
BsrGI TGTACA 1 cut(s) 590
BssAI RCCGGY 1 cut(s) 551
BssECI CCNNGG 3 cut(s) 157, 220, 260
BssMI GATC 3 cut(s) 105, 871, 992
BssT1I CCWWGG 1 cut(s) 260
Bst2UI CCWGG 1 cut(s) 256
Bst4CI ACNGT 4 cut(s) 514, 607, 659, 791
Bst6I CTCTTC 1 cut(s) 749
BstAUI TGTACA 1 cut(s) 590
BstC8I GCNNGC 1 cut(s) 765
BstDEI CTNAG 2 cut(s) 647, 821
BstDSI CCRYGG 1 cut(s) 157
BstEII GGTNACC 1 cut(s) 836
BstF5I GGATG 2 cut(s) 53, 851
BstKTI GATC 3 cut(s) 108, 874, 995
BstMBI GATC 3 cut(s) 105, 871, 992
BstMWI GCNNNNNNNGC 1 cut(s) 683
BstNI CCWGG 1 cut(s) 256
BstNSI RCATGY 1 cut(s) 1049
BstPI GGTNACC 1 cut(s) 836
BstSCI CCNGG 2 cut(s) 219, 254
BstV1I GCAGC 1 cut(s) 793
BstX2I RGATCY 1 cut(s) 871
BstXI CCANNNNNNTGG 1 cut(s) 858
BstYI RGATCY 1 cut(s) 871
BsuRI GGCC 5 cut(s) 214, 259, 316, 535, 763
BtgI CCRYGG 1 cut(s) 157
BtsCI GGATG 2 cut(s) 53, 851
BtsIMutI CAGTG 1 cut(s) 796
Cac8I GCNNGC 1 cut(s) 765
Cfr10I RCCGGY 1 cut(s) 551
Cfr13I GGNCC 6 cut(s) 131, 167, 212, 719, 783, 935
CseI GACGC 1 cut(s) 949
Csp6I GTAC 1 cut(s) 591
CspAI ACCGGT 1 cut(s) 551
CspCI CAANNNNNGTGG 4 cut(s) 306, 341, 621, 656
CviAII CATG 1 cut(s) 1046
CviQI GTAC 1 cut(s) 591
DdeI CTNAG 2 cut(s) 647, 821
DpnI GATC 3 cut(s) 107, 873, 994
DpnII GATC 3 cut(s) 105, 871, 992
DrdI GACNNNNNNGTC 1 cut(s) 946
DseDI GACNNNNNNGTC 1 cut(s) 946
Eam1104I CTCTTC 1 cut(s) 749
EarI CTCTTC 1 cut(s) 749
EciI GGCGGA 1 cut(s) 673
Ecl136II GAGCTC 1 cut(s) 112
Eco130I CCWWGG 1 cut(s) 260
Eco24I GRGCYC 1 cut(s) 114
Eco47I GGWCC 5 cut(s) 131, 167, 719, 783, 935
Eco53kI GAGCTC 1 cut(s) 112
Eco91I GGTNACC 1 cut(s) 836
EcoICRI GAGCTC 1 cut(s) 112
EcoO109I RGGNCCY 3 cut(s) 167, 212, 719
EcoO65I GGTNACC 1 cut(s) 836
EcoRI GAATTC 1 cut(s) 195
EcoRII CCWGG 1 cut(s) 254
EcoT14I CCWWGG 1 cut(s) 260
EcoT38I GRGCYC 1 cut(s) 114
ErhI CCWWGG 1 cut(s) 260
FaeI CATG 1 cut(s) 1049
FaqI GGGAC 1 cut(s) 425
FatI CATG 1 cut(s) 1045
Fnu4HI GCNGC 1 cut(s) 807
FokI GGATG 2 cut(s) 40, 838
FriOI GRGCYC 1 cut(s) 114
Fsp4HI GCNGC 1 cut(s) 807
FspBI CTAG 3 cut(s) 47, 320, 671
GluI GCNGC 1 cut(s) 807
HaeIII GGCC 5 cut(s) 214, 259, 316, 535, 763
HapII CCGG 5 cut(s) 134, 220, 552, 564, 875
HgaI GACGC 1 cut(s) 949
Hin1II CATG 1 cut(s) 1049
HincII GTYRAC 2 cut(s) 85, 864
HindII GTYRAC 2 cut(s) 85, 864
HindIII AAGCTT 1 cut(s) 704
HinfI GANTC 3 cut(s) 438, 680, 947
HpaII CCGG 5 cut(s) 134, 220, 552, 564, 875
HphI GGTGA 3 cut(s) 566, 613, 806
Hpy166II GTNNAC 4 cut(s) 85, 350, 517, 864
Hpy188I TCNGA 4 cut(s) 105, 187, 456, 939
Hpy188III TCNNGA 3 cut(s) 134, 464, 731
Hpy8I GTNNAC 4 cut(s) 85, 350, 517, 864
Hpy99I CGWCG 2 cut(s) 737, 943
HpyAV CCTTC 4 cut(s) 306, 519, 561, 592
HpyCH4III ACNGT 4 cut(s) 514, 607, 659, 791
HpyCH4IV ACGT 2 cut(s) 735, 906
HpyCH4V TGCA 4 cut(s) 577, 632, 809, 1049
HpyF10VI GCNNNNNNNGC 1 cut(s) 683
HpyF3I CTNAG 2 cut(s) 647, 821
HpySE526I ACGT 2 cut(s) 735, 906
Hsp92II CATG 1 cut(s) 1049
Kpn2I TCCGGA 1 cut(s) 133
Kzo9I GATC 3 cut(s) 105, 871, 992
LmnI GCTCC 1 cut(s) 117
Lsp1109I GCAGC 1 cut(s) 793
LweI GCATC 3 cut(s) 59, 74, 355
MaeI CTAG 3 cut(s) 47, 320, 671
MaeII ACGT 2 cut(s) 735, 906
MaeIII GTNAC 3 cut(s) 554, 601, 836
MalI GATC 3 cut(s) 107, 873, 994
MboI GATC 3 cut(s) 105, 871, 992
MboII GAAGA 5 cut(s) 22, 204, 691, 736, 977
MfeI CAATTG 1 cut(s) 543
MflI RGATCY 1 cut(s) 871
MhlI GDGCHC 1 cut(s) 114
MluCI AATT 5 cut(s) 94, 195, 486, 543, 797
MlyI GAGTC 2 cut(s) 447, 956
MmeI TCCRAC 3 cut(s) 157, 962, 994
MroI TCCGGA 1 cut(s) 133
MseI TTAA 3 cut(s) 41, 383, 777
MslI CAYNNNNRTG 1 cut(s) 856
MspI CCGG 5 cut(s) 134, 220, 552, 564, 875
MspR9I CCNGG 2 cut(s) 221, 256
MunI CAATTG 1 cut(s) 543
Mva1269I GAATGC 1 cut(s) 463
MvaI CCWGG 1 cut(s) 256
MwoI GCNNNNNNNGC 1 cut(s) 683
NciI CCSGG 1 cut(s) 221
NdeII GATC 3 cut(s) 105, 871, 992
NlaIII CATG 1 cut(s) 1049
NlaIV GGNNCC 1 cut(s) 721
NmuCI GTSAC 2 cut(s) 554, 601
NspI RCATGY 1 cut(s) 1049
PctI GAATGC 1 cut(s) 463
PfeI GAWTC 1 cut(s) 680
PflMI CCANNNNNTGG 1 cut(s) 308
PinAI ACCGGT 1 cut(s) 551
PkrI GCNGC 1 cut(s) 808
PleI GAGTC 2 cut(s) 446, 955
PpsI GAGTC 2 cut(s) 446, 955
PpuMI RGGWCCY 2 cut(s) 167, 719
Psp124BI GAGCTC 1 cut(s) 114
Psp5II RGGWCCY 2 cut(s) 167, 719
Psp6I CCWGG 1 cut(s) 254
PspEI GGTNACC 1 cut(s) 836
PspGI CCWGG 1 cut(s) 254
PspN4I GGNNCC 1 cut(s) 721
PspPI GGNCC 6 cut(s) 131, 167, 212, 719, 783, 935
PspPPI RGGWCCY 2 cut(s) 167, 719
PsuI RGATCY 1 cut(s) 871
RsaI GTAC 1 cut(s) 592
RsaNI GTAC 1 cut(s) 591
RseI CAYNNNNRTG 1 cut(s) 856
SacI GAGCTC 1 cut(s) 114
SaqAI TTAA 3 cut(s) 41, 383, 777
SatI GCNGC 1 cut(s) 807
Sau3AI GATC 3 cut(s) 105, 871, 992
Sau96I GGNCC 6 cut(s) 131, 167, 212, 719, 783, 935
SchI GAGTC 2 cut(s) 447, 956
ScrFI CCNGG 2 cut(s) 221, 256
SduI GDGCHC 1 cut(s) 114
SfaNI GCATC 3 cut(s) 59, 74, 355
SgrAI CRCCGGYG 1 cut(s) 551
SinI GGWCC 5 cut(s) 131, 167, 719, 783, 935
SmiMI CAYNNNNRTG 1 cut(s) 856
SmlI CTYRAG 1 cut(s) 944
SmoI CTYRAG 1 cut(s) 944
Sse9I AATT 5 cut(s) 94, 195, 486, 543, 797
SsiI CCGC 1 cut(s) 684
SspMI CTAG 3 cut(s) 47, 320, 671
SstI GAGCTC 1 cut(s) 114
StyD4I CCNGG 2 cut(s) 219, 254
StyI CCWWGG 1 cut(s) 260
TaaI ACNGT 4 cut(s) 514, 607, 659, 791
TaiI ACGT 2 cut(s) 738, 909
TaqI TCGA 4 cut(s) 108, 150, 615, 715
TasI AATT 5 cut(s) 94, 195, 486, 543, 797
TatI WGTACW 1 cut(s) 590
TfiI GAWTC 1 cut(s) 680
Tru1I TTAA 3 cut(s) 41, 383, 777
Tru9I TTAA 3 cut(s) 41, 383, 777
TscAI CASTG 1 cut(s) 796
TseFI GTSAC 2 cut(s) 554, 601
TseI GCWGC 1 cut(s) 806
Tsp45I GTSAC 2 cut(s) 554, 601
TspDTI ATGAA 2 cut(s) 17, 909
TspGWI ACGGA 3 cut(s) 245, 437, 990
TspRI CASTG 1 cut(s) 796
Van91I CCANNNNNTGG 1 cut(s) 308
VpaK11BI GGWCC 5 cut(s) 131, 167, 719, 783, 935
XapI RAATTY 2 cut(s) 195, 797
XceI RCATGY 1 cut(s) 1049
XspI CTAG 3 cut(s) 47, 320, 671
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.