Rroxscaffold_5G00377800

strictosidine

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0000674
Physical Location & Seq
Reverse (-)
58400927 .. 58404011
3085 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00377800.1

Sequence Viewer

Length: 1044 bp
ATGAACCCTCCTCCTTTTGCTTCATTCTACTTGCTCTTGCCTTGTTTACTGATCATCCTAGTATCAACCTCGACATCCAAACCAGCATTAGCTTTCTCCATTGCTAGCCTCCGGAAATACCACCAACTCGAGCTCCCTTATAACGTAGTCGGCCCTGAAAGCGTTGCGTTCGACTGCCGTGGAGAAGGACCTTATGTTGGGGTTTCAGATGGTAGAATTCTCAAATGGGAGGAACCGCACAACGGCTGGAGAGAGTTTGGCTACACTTCCCCGAATAGGCAGAGGAAGTTATGCGATGGCTCAACCAACAAGATAAATGAACCGATATGCGGAAGGCCACTGGGTCTAAAGTTTAACCCTACAACATGTGAACTGTATATAGCAGATGCCTATTTTGGACTCTTGAAGACGGGACCAAATGGTGGTCGTCCTCATCAACTCGCCACTTCTGCAGGAGGAGTCCCCTTCCATTTCTTAAATGCTTTGGACATCGATGACGAATCTGGAATGGTTTATTTTACGGACACTAGCCTTTATTTCCAAAGAAGTGTTTGGATATTGTCCATCATCTCCGGTGACAGAACTGGAAGGTTAATGCAATACGACCCTAGTACAAAGAAGGTGACTGTGTTGCTCAAGGGATTGGCATTTCCAAATGGTGTGGCTCTAACTAAGGACAAATCTTTCCTCCTCCTAGCCGAAACTGGCACGCTGAAAATCCTCAGGTTGTGGCTTCGAGGACCCAAATCTAATGCCTTGGAACTTTTTGCTCAACTAGTCGGGTTCCCAGATAACATCAAGAGAAACAACAATGGAGAATTCTGGGTTGCGATCAACAACGGAAGAGGAAGATCAATATCTCGGACTCTGCTAGGAGATCCTGTAGGAGCAAAGTTTGATGAAGAAGGAAACGTTTTGGAGGTGTTAGATGGAGAGGATAGTACGGCAGCGTTGCTTCAGTCTGTTAGTGAAGTTGAAGAACACAACGGAAGGTTGTGGATAGGATCGGTGGAAAATTCATATGTTGGTGTTGGCTTGTTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Str_synth

Protein Analysis

347

Amino Acids

38.19

Weight (kDa)

6.25

Isoelectric Point (pI)

32.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Str_synth PF03088 160 - 247 4.2e-29 Strictosidine synthase
SGL PF08450 193 - 282 2.4e-07 SMP-30/Gluconolactonase/LRE-like region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 141
AccB7I CCANNNNNTGG 1 cut(s) 422
AccIII TCCGGA 1 cut(s) 111
AciI CCGC 2 cut(s) 236, 330
AclI AACGTT 1 cut(s) 912
AclWI GGATC 2 cut(s) 872, 1012
AcsI RAATTY 3 cut(s) 216, 818, 1015
AcuI CTGAAG 1 cut(s) 941
AfaI GTAC 2 cut(s) 613, 943
AfiI CCNNNNNNNGG 5 cut(s) 197, 242, 276, 329, 422
AflIII ACRYGT 1 cut(s) 365
AgsI TTSAA 2 cut(s) 406, 977
AhlI ACTAGT 1 cut(s) 775
AluBI AGCT 2 cut(s) 92, 133
AluI AGCT 2 cut(s) 92, 133
Alw21I GWGCWC 1 cut(s) 135
AlwI GGATC 2 cut(s) 872, 1012
Ama87I CYCGRG 1 cut(s) 128
Aor13HI TCCGGA 1 cut(s) 111
AoxI GGCC 2 cut(s) 151, 335
ApeKI GCWGC 1 cut(s) 947
ApoI RAATTY 3 cut(s) 216, 818, 1015
AspS9I GGNCC 4 cut(s) 152, 188, 413, 740
AsuHPI GGTGA 2 cut(s) 587, 634
AsuNHI GCTAGC 1 cut(s) 104
AvaI CYCGRG 1 cut(s) 128
AvaII GGWCC 3 cut(s) 188, 413, 740
AxyI CCTNAGG 1 cut(s) 722
BanII GRGCYC 1 cut(s) 135
BbsI GAAGAC 1 cut(s) 413
Bbv12I GWGCWC 1 cut(s) 135
BbvI GCAGC 1 cut(s) 959
BccI CCATC 4 cut(s) 203, 290, 572, 923
BceAI ACGGC 3 cut(s) 162, 259, 960
BclI TGATCA 1 cut(s) 51
BcuI ACTAGT 1 cut(s) 775
BfaI CTAG 7 cut(s) 59, 105, 528, 609, 695, 776, 872
BfmI CTRYAG 2 cut(s) 450, 882
BisI GCNGC 1 cut(s) 948
BlsI GCNGC 1 cut(s) 949
Bme18I GGWCC 3 cut(s) 188, 413, 740
BmeT110I CYCGRG 1 cut(s) 128
BmgT120I GGNCC 4 cut(s) 152, 188, 413, 740
BmiI GGNNCC 4 cut(s) 234, 414, 742, 785
BmrI ACTGGG 1 cut(s) 350
BmsI GCATC 1 cut(s) 376
BmtI GCTAGC 1 cut(s) 108
BmuI ACTGGG 1 cut(s) 350
BpiI GAAGAC 1 cut(s) 413
BpmI CTGGAG 1 cut(s) 268
BpuEI CTTGAG 1 cut(s) 620
Bsa29I ATCGAT 1 cut(s) 492
BsaBI GATNNNNATC 1 cut(s) 856
BsaJI CCNNGG 2 cut(s) 178, 756
BsaWI WCCGGW 2 cut(s) 111, 572
BsaXI ACNNNNNCTCC 4 cut(s) 117, 147, 867, 897
Bsc4I CCNNNNNNNGG 5 cut(s) 197, 242, 276, 329, 422
Bse1I ACTGG 3 cut(s) 345, 589, 709
Bse21I CCTNAGG 1 cut(s) 722
Bse3DI GCAATG 1 cut(s) 99
Bse8I GATNNNNATC 1 cut(s) 856
BseAI TCCGGA 1 cut(s) 111
BseCI ATCGAT 1 cut(s) 492
BseDI CCNNGG 2 cut(s) 178, 756
BseGI GGATG 2 cut(s) 54, 74
BseJI GATNNNNATC 1 cut(s) 856
BseLI CCNNNNNNNGG 5 cut(s) 197, 242, 276, 329, 422
BseMI GCAATG 1 cut(s) 99
BseMII CTCAG 1 cut(s) 736
BseNI ACTGG 3 cut(s) 345, 589, 709
BseRI GAGGAG 2 cut(s) 471, 680
BseXI GCAGC 1 cut(s) 959
BshFI GGCC 2 cut(s) 153, 337
BshVI ATCGAT 1 cut(s) 492
BsiHKAI GWGCWC 1 cut(s) 135
BsiHKCI CYCGRG 1 cut(s) 128
BsiSI CCGG 2 cut(s) 112, 573
BslFI GGGAC 2 cut(s) 426, 446
BslI CCNNNNNNNGG 5 cut(s) 197, 242, 276, 329, 422
BsmFI GGGAC 2 cut(s) 426, 446
BsnI GGCC 2 cut(s) 153, 337
BsoBI CYCGRG 1 cut(s) 128
Bsp1286I GDGCHC 1 cut(s) 135
Bsp13I TCCGGA 1 cut(s) 111
Bsp143I GATC 5 cut(s) 51, 831, 851, 877, 1004
BspACI CCGC 2 cut(s) 236, 330
BspANI GGCC 2 cut(s) 153, 337
BspCNI CTCAG 1 cut(s) 735
BspDI ATCGAT 1 cut(s) 492
BspEI TCCGGA 1 cut(s) 111
BspLI GGNNCC 4 cut(s) 234, 414, 742, 785
BspMAI CTGCAG 1 cut(s) 454
BspOI GCTAGC 1 cut(s) 108
BspPI GGATC 2 cut(s) 872, 1012
BsrDI GCAATG 1 cut(s) 99
BsrI ACTGG 3 cut(s) 345, 589, 709
BssECI CCNNGG 2 cut(s) 178, 756
BssMI GATC 5 cut(s) 51, 831, 851, 877, 1004
BssT1I CCWWGG 1 cut(s) 756
Bst4CI ACNGT 2 cut(s) 375, 628
Bst6I CTCTTC 1 cut(s) 838
BstC8I GCNNGC 2 cut(s) 106, 710
BstDEI CTNAG 2 cut(s) 672, 722
BstDSI CCRYGG 1 cut(s) 178
BstF5I GGATG 2 cut(s) 54, 74
BstKTI GATC 5 cut(s) 54, 834, 854, 880, 1007
BstMBI GATC 5 cut(s) 51, 831, 851, 877, 1004
BstMWI GCNNNNNNNGC 2 cut(s) 159, 449
BstNSI RCATGY 1 cut(s) 369
BstSFI CTRYAG 2 cut(s) 450, 882
BstV1I GCAGC 1 cut(s) 959
BstV2I GAAGAC 1 cut(s) 413
BstX2I RGATCY 1 cut(s) 877
BstYI RGATCY 1 cut(s) 877
Bsu15I ATCGAT 1 cut(s) 492
Bsu36I CCTNAGG 1 cut(s) 722
BsuRI GGCC 2 cut(s) 153, 337
BsuTUI ATCGAT 1 cut(s) 492
BtgI CCRYGG 1 cut(s) 178
BtgZI GCGATG 1 cut(s) 309
BtsCI GGATG 2 cut(s) 54, 74
BtsIMutI CAGTG 1 cut(s) 338
Cac8I GCNNGC 2 cut(s) 106, 710
Cfr13I GGNCC 4 cut(s) 152, 188, 413, 740
ClaI ATCGAT 1 cut(s) 492
Csp6I GTAC 2 cut(s) 612, 942
CspCI CAANNNNNGTGG 2 cut(s) 642, 677
CviAII CATG 1 cut(s) 366
CviQI GTAC 2 cut(s) 612, 942
DdeI CTNAG 2 cut(s) 672, 722
DpnI GATC 5 cut(s) 53, 833, 853, 879, 1006
DpnII GATC 5 cut(s) 51, 831, 851, 877, 1004
Eam1104I CTCTTC 1 cut(s) 838
EarI CTCTTC 1 cut(s) 838
Ecl136II GAGCTC 1 cut(s) 133
Eco130I CCWWGG 1 cut(s) 756
Eco24I GRGCYC 1 cut(s) 135
Eco47I GGWCC 3 cut(s) 188, 413, 740
Eco53kI GAGCTC 1 cut(s) 133
Eco57I CTGAAG 1 cut(s) 941
Eco81I CCTNAGG 1 cut(s) 722
Eco88I CYCGRG 1 cut(s) 128
EcoICRI GAGCTC 1 cut(s) 133
EcoO109I RGGNCCY 2 cut(s) 188, 740
EcoRI GAATTC 2 cut(s) 216, 818
EcoT14I CCWWGG 1 cut(s) 756
EcoT38I GRGCYC 1 cut(s) 135
ErhI CCWWGG 1 cut(s) 756
FaeI CATG 1 cut(s) 369
FaiI YATR 9 cut(s) 141, 195, 292, 328, 367, 378, 380, 1021, 1023
FaqI GGGAC 2 cut(s) 426, 446
FatI CATG 1 cut(s) 365
FauNDI CATATG 1 cut(s) 1021
FbaI TGATCA 1 cut(s) 51
Fnu4HI GCNGC 1 cut(s) 948
FokI GGATG 2 cut(s) 41, 61
FriOI GRGCYC 1 cut(s) 135
Fsp4HI GCNGC 1 cut(s) 948
FspBI CTAG 7 cut(s) 59, 105, 528, 609, 695, 776, 872
GluI GCNGC 1 cut(s) 948
GsuI CTGGAG 1 cut(s) 268
HaeIII GGCC 2 cut(s) 153, 337
HapII CCGG 2 cut(s) 112, 573
Hin1II CATG 1 cut(s) 369
HinfI GANTC 4 cut(s) 399, 459, 500, 865
HpaII CCGG 2 cut(s) 112, 573
HphI GGTGA 2 cut(s) 587, 634
Hpy166II GTNNAC 2 cut(s) 47, 371
Hpy188I TCNGA 2 cut(s) 208, 864
Hpy188III TCNNGA 4 cut(s) 112, 403, 504, 799
Hpy8I GTNNAC 2 cut(s) 47, 371
HpyAV CCTTC 7 cut(s) 179, 327, 475, 582, 613, 899, 984
HpyCH4III ACNGT 2 cut(s) 375, 628
HpyCH4IV ACGT 2 cut(s) 144, 912
HpyCH4V TGCA 2 cut(s) 452, 598
HpyF10VI GCNNNNNNNGC 2 cut(s) 159, 449
HpyF3I CTNAG 2 cut(s) 672, 722
HpySE526I ACGT 2 cut(s) 144, 912
Hsp92II CATG 1 cut(s) 369
Kpn2I TCCGGA 1 cut(s) 111
Ksp22I TGATCA 1 cut(s) 51
Kzo9I GATC 5 cut(s) 51, 831, 851, 877, 1004
LmnI GCTCC 2 cut(s) 138, 887
Lsp1109I GCAGC 1 cut(s) 959
LweI GCATC 1 cut(s) 376
MaeI CTAG 7 cut(s) 59, 105, 528, 609, 695, 776, 872
MaeII ACGT 2 cut(s) 144, 912
MaeIII GTNAC 2 cut(s) 575, 622
MalI GATC 5 cut(s) 53, 833, 853, 879, 1006
MboI GATC 5 cut(s) 51, 831, 851, 877, 1004
MboII GAAGA 5 cut(s) 418, 855, 861, 914, 989
MflI RGATCY 1 cut(s) 877
MhlI GDGCHC 1 cut(s) 135
MluCI AATT 3 cut(s) 216, 818, 1015
MlyI GAGTC 3 cut(s) 393, 468, 859
MroI TCCGGA 1 cut(s) 111
MseI TTAA 3 cut(s) 354, 476, 593
MspI CCGG 2 cut(s) 112, 573
MwoI GCNNNNNNNGC 2 cut(s) 159, 449
NdeI CATATG 1 cut(s) 1021
NdeII GATC 5 cut(s) 51, 831, 851, 877, 1004
NheI GCTAGC 1 cut(s) 104
NlaIII CATG 1 cut(s) 369
NlaIV GGNNCC 4 cut(s) 234, 414, 742, 785
NmuCI GTSAC 2 cut(s) 575, 622
NspI RCATGY 1 cut(s) 369
PaeR7I CTCGAG 1 cut(s) 128
PciI ACATGT 1 cut(s) 365
PfeI GAWTC 1 cut(s) 500
PflMI CCANNNNNTGG 1 cut(s) 422
PkrI GCNGC 1 cut(s) 949
PleI GAGTC 3 cut(s) 393, 467, 859
PpsI GAGTC 3 cut(s) 393, 467, 859
PpuMI RGGWCCY 2 cut(s) 188, 740
PscI ACATGT 1 cut(s) 365
PsiI TTATAA 1 cut(s) 141
Psp124BI GAGCTC 1 cut(s) 135
Psp1406I AACGTT 1 cut(s) 912
Psp5II RGGWCCY 2 cut(s) 188, 740
PspN4I GGNNCC 4 cut(s) 234, 414, 742, 785
PspPI GGNCC 4 cut(s) 152, 188, 413, 740
PspPPI RGGWCCY 2 cut(s) 188, 740
PspXI VCTCGAGB 1 cut(s) 128
PstI CTGCAG 1 cut(s) 454
PsuI RGATCY 1 cut(s) 877
RsaI GTAC 2 cut(s) 613, 943
RsaNI GTAC 2 cut(s) 612, 942
SacI GAGCTC 1 cut(s) 135
SaqAI TTAA 3 cut(s) 354, 476, 593
SatI GCNGC 1 cut(s) 948
Sau3AI GATC 5 cut(s) 51, 831, 851, 877, 1004
Sau96I GGNCC 4 cut(s) 152, 188, 413, 740
SchI GAGTC 3 cut(s) 393, 468, 859
SduI GDGCHC 1 cut(s) 135
SfaNI GCATC 1 cut(s) 376
SfcI CTRYAG 2 cut(s) 450, 882
Sfr274I CTCGAG 1 cut(s) 128
SinI GGWCC 3 cut(s) 188, 413, 740
SlaI CTCGAG 1 cut(s) 128
SmlI CTYRAG 2 cut(s) 128, 635
SmoI CTYRAG 2 cut(s) 128, 635
SpeI ACTAGT 1 cut(s) 775
Sse9I AATT 3 cut(s) 216, 818, 1015
SsiI CCGC 2 cut(s) 236, 330
SspMI CTAG 7 cut(s) 59, 105, 528, 609, 695, 776, 872
SstI GAGCTC 1 cut(s) 135
StyI CCWWGG 1 cut(s) 756
TaaI ACNGT 2 cut(s) 375, 628
TaiI ACGT 2 cut(s) 147, 915
TaqI TCGA 5 cut(s) 71, 129, 171, 492, 736
TasI AATT 3 cut(s) 216, 818, 1015
TatI WGTACW 1 cut(s) 611
TfiI GAWTC 1 cut(s) 500
Tru1I TTAA 3 cut(s) 354, 476, 593
Tru9I TTAA 3 cut(s) 354, 476, 593
TscAI CASTG 1 cut(s) 345
TseFI GTSAC 2 cut(s) 575, 622
TseI GCWGC 1 cut(s) 947
Tsp45I GTSAC 2 cut(s) 575, 622
TspDTI ATGAA 5 cut(s) 12, 17, 333, 915, 1008
TspGWI ACGGA 3 cut(s) 536, 855, 1002
TspRI CASTG 1 cut(s) 345
Van91I CCANNNNNTGG 1 cut(s) 422
VpaK11BI GGWCC 3 cut(s) 188, 413, 740
XapI RAATTY 3 cut(s) 216, 818, 1015
XceI RCATGY 1 cut(s) 369
XhoI CTCGAG 1 cut(s) 128
XspI CTAG 7 cut(s) 59, 105, 528, 609, 695, 776, 872
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.