FvH4_7g01710

strictosidine

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0000674
Physical Location & Seq
Forward (+)
2078158 .. 2079120
963 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g01710.t1

Sequence Viewer

Length: 873 bp
ATGAAATCAGCTCTTGTTATTGTCTTCTTCTTGTTCTTTTTGTCCGGTAACCTAGCCCATGCAGCATATTTTTCACTCCCAAACCTGAACCATCATGAAATACCCATCCATGTACCAGAACCCGAAAGCATTGCTTTCGACTGCAATGGCGCACCCTACGTGGGTGTGGCTGATGGCAGGATCTTCAAGTGGCTAGGACTCCCGTTTGGGCCACTTGGTTTGAAATTTGACCTCACAACTTGCGAACTGTATATCGCAGATGCATACTTCGGGCTTCTAAAGACAGGACCATATGGTGGAGCAGCTCAAGCACTTGCAACATCGGCTGAAGGATTTCCTTTCCGTTTCTTGAATGGTGTGGACATTGATCACCACGGTGGAATGGTCTACTTCACAGACACCAGTACTATTTATCAGAGAAGGAATTGGGAGTTGTCAGTCAGTACAGGTGATAAAACTGGAAGGTTGATGCGATACGATCCATCAACAAAGAATGTGACAGTGTTGCTTCATGGATTGTCTTTTGCAAACGGGGTGGCACTAAGCAAAGATGGTGATTTTGTTCTGGTGACTCAAACCACTGCAAAGAACATCTTGAGGTATTGGCTTCGAGGGCCTAGAGCCAACACTGTTGATACTTTTTTCAACTTGCGAGGAGCCCCAGACAACATCAACAGAAACATCAATGGAGGATTCTGGGTCGCCGAAAACAGTGCTGGAATAGGTAAAGCGGTGAAGCTTGACGCCCAAGGAAATTTTCTGGATGTGGTAGATGGACTCAACCCTATGAGTCATGTTGAGCAGCGTTATGGAAACCTTTGGATAGGATCTGTTGTTAATAACTTTGTTGGGGTTATTCTTCATTTTGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Str_synth

Protein Analysis

291

Amino Acids

31.82

Weight (kDa)

6.49

Isoelectric Point (pI)

18.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Str_synth PF03088 118 - 205 2.1e-31 Strictosidine synthase
SGL PF08450 149 - 257 2.2e-10 SMP-30/Gluconolactonase/LRE-like region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 296
AccI GTMKAC 1 cut(s) 387
AciI CCGC 1 cut(s) 731
AclWI GGATC 3 cut(s) 188, 473, 835
AcsI RAATTY 2 cut(s) 224, 754
AcuI CTGAAG 1 cut(s) 348
AcyI GRCGYC 1 cut(s) 744
AfaI GTAC 3 cut(s) 114, 406, 445
AfiI CCNNNNNNNGG 2 cut(s) 161, 296
AgsI TTSAA 4 cut(s) 187, 223, 352, 646
AjuI GAANNNNNNNTTGG 2 cut(s) 741, 773
AleI CACNNNNGTG 1 cut(s) 375
AluBI AGCT 3 cut(s) 11, 305, 739
AluI AGCT 3 cut(s) 11, 305, 739
AlwI GGATC 3 cut(s) 188, 473, 835
AoxI GGCC 2 cut(s) 209, 614
ApeKI GCWGC 3 cut(s) 62, 302, 802
ApoI RAATTY 2 cut(s) 224, 754
Asp700I GAANNNNTTC 1 cut(s) 333
AspLEI GCGC 1 cut(s) 152
AspS9I GGNCC 3 cut(s) 209, 287, 614
AsuHPI GGTGA 5 cut(s) 362, 461, 566, 580, 745
AvaII GGWCC 1 cut(s) 287
BanII GRGCYC 1 cut(s) 661
BbsI GAAGAC 1 cut(s) 16
BbvI GCAGC 3 cut(s) 74, 314, 814
BccI CCATC 6 cut(s) 99, 113, 167, 490, 545, 767
BcgI CGANNNNNNTGC 6 cut(s) 113, 118, 147, 152, 695, 729
BclI TGATCA 1 cut(s) 367
BfaI CTAG 3 cut(s) 53, 194, 618
BisI GCNGC 3 cut(s) 63, 303, 803
BlsI GCNGC 3 cut(s) 64, 304, 804
BmcAI AGTACT 1 cut(s) 406
Bme18I GGWCC 1 cut(s) 287
BmgT120I GGNCC 3 cut(s) 209, 287, 614
BmiI GGNNCC 1 cut(s) 658
BmsI GCATC 2 cut(s) 250, 459
BpiI GAAGAC 1 cut(s) 16
BpuEI CTTGAG 2 cut(s) 291, 616
BsaAI YACGTR 1 cut(s) 160
BsaHI GRCGYC 1 cut(s) 744
BsaJI CCNNGG 2 cut(s) 373, 748
BsaWI WCCGGW 1 cut(s) 44
Bsc4I CCNNNNNNNGG 2 cut(s) 161, 296
Bse1I ACTGG 2 cut(s) 402, 463
Bse3DI GCAATG 2 cut(s) 129, 151
BseDI CCNNGG 2 cut(s) 373, 748
BseGI GGATG 2 cut(s) 105, 769
BseLI CCNNNNNNNGG 2 cut(s) 161, 296
BseMI GCAATG 2 cut(s) 129, 151
BseNI ACTGG 2 cut(s) 402, 463
BseRI GAGGAG 1 cut(s) 669
BseXI GCAGC 3 cut(s) 74, 314, 814
BshFI GGCC 2 cut(s) 211, 616
BsiSI CCGG 1 cut(s) 45
BslI CCNNNNNNNGG 2 cut(s) 161, 296
BsnI GGCC 2 cut(s) 211, 616
Bsp1286I GDGCHC 1 cut(s) 661
Bsp143I GATC 4 cut(s) 180, 367, 478, 827
BspACI CCGC 1 cut(s) 731
BspANI GGCC 2 cut(s) 211, 616
BspHI TCATGA 1 cut(s) 94
BspLI GGNNCC 1 cut(s) 658
BspPI GGATC 3 cut(s) 188, 473, 835
BsrDI GCAATG 2 cut(s) 129, 151
BsrI ACTGG 2 cut(s) 402, 463
BssECI CCNNGG 2 cut(s) 373, 748
BssMI GATC 4 cut(s) 180, 367, 478, 827
BssNI GRCGYC 1 cut(s) 744
BssT1I CCWWGG 1 cut(s) 748
Bst4CI ACNGT 5 cut(s) 249, 377, 502, 631, 713
BstACI GRCGYC 1 cut(s) 744
BstBAI YACGTR 1 cut(s) 160
BstDEI CTNAG 1 cut(s) 542
BstDSI CCRYGG 1 cut(s) 373
BstEII GGTNACC 1 cut(s) 47
BstF5I GGATG 2 cut(s) 105, 769
BstHHI GCGC 1 cut(s) 152
BstKTI GATC 4 cut(s) 183, 370, 481, 830
BstMBI GATC 4 cut(s) 180, 367, 478, 827
BstMWI GCNNNNNNNGC 4 cut(s) 62, 308, 323, 613
BstPI GGTNACC 1 cut(s) 47
BstV1I GCAGC 3 cut(s) 74, 314, 814
BstV2I GAAGAC 1 cut(s) 16
BstX2I RGATCY 2 cut(s) 180, 827
BstYI RGATCY 2 cut(s) 180, 827
BsuRI GGCC 2 cut(s) 211, 616
BtgI CCRYGG 1 cut(s) 373
BtsCI GGATG 2 cut(s) 105, 769
BtsI GCAGTG 1 cut(s) 579
BtsIMutI CAGTG 4 cut(s) 507, 579, 627, 718
CciI TCATGA 1 cut(s) 94
CfoI GCGC 1 cut(s) 152
Cfr13I GGNCC 3 cut(s) 209, 287, 614
CseI GACGC 1 cut(s) 752
Csp6I GTAC 3 cut(s) 113, 405, 444
CspCI CAANNNNNGTGG 4 cut(s) 201, 236, 516, 551
CviAII CATG 5 cut(s) 59, 95, 110, 512, 794
CviQI GTAC 3 cut(s) 113, 405, 444
DdeI CTNAG 1 cut(s) 542
DpnI GATC 4 cut(s) 182, 369, 480, 829
DpnII GATC 4 cut(s) 180, 367, 478, 827
Eco130I CCWWGG 1 cut(s) 748
Eco24I GRGCYC 1 cut(s) 661
Eco47I GGWCC 1 cut(s) 287
Eco57I CTGAAG 1 cut(s) 348
Eco91I GGTNACC 1 cut(s) 47
EcoO109I RGGNCCY 1 cut(s) 614
EcoO65I GGTNACC 1 cut(s) 47
EcoT14I CCWWGG 1 cut(s) 748
EcoT22I ATGCAT 1 cut(s) 265
EcoT38I GRGCYC 1 cut(s) 661
ErhI CCWWGG 1 cut(s) 748
FaeI CATG 5 cut(s) 62, 98, 113, 515, 797
FalI AAGNNNNNCTT 4 cut(s) 118, 150, 578, 610
FatI CATG 5 cut(s) 58, 94, 109, 511, 793
FauNDI CATATG 1 cut(s) 292
FbaI TGATCA 1 cut(s) 367
FblI GTMKAC 1 cut(s) 387
Fnu4HI GCNGC 3 cut(s) 63, 303, 803
FokI GGATG 2 cut(s) 92, 776
FriOI GRGCYC 1 cut(s) 661
Fsp4HI GCNGC 3 cut(s) 63, 303, 803
FspBI CTAG 3 cut(s) 53, 194, 618
GlaI GCGC 1 cut(s) 151
GluI GCNGC 3 cut(s) 63, 303, 803
HaeIII GGCC 2 cut(s) 211, 616
HapII CCGG 1 cut(s) 45
HgaI GACGC 1 cut(s) 752
HhaI GCGC 1 cut(s) 152
Hin1I GRCGYC 1 cut(s) 744
Hin1II CATG 5 cut(s) 62, 98, 113, 515, 797
Hin6I GCGC 1 cut(s) 150
HinP1I GCGC 1 cut(s) 150
HindIII AAGCTT 1 cut(s) 737
HinfI GANTC 5 cut(s) 198, 571, 693, 777, 790
HpaII CCGG 1 cut(s) 45
HphI GGTGA 5 cut(s) 362, 461, 566, 580, 745
Hpy166II GTNNAC 2 cut(s) 361, 388
Hpy188I TCNGA 1 cut(s) 417
Hpy188III TCNNGA 4 cut(s) 95, 349, 595, 761
Hpy8I GTNNAC 2 cut(s) 361, 388
HpyAV CCTTC 3 cut(s) 323, 414, 456
HpyCH4III ACNGT 5 cut(s) 249, 377, 502, 631, 713
HpyCH4IV ACGT 1 cut(s) 159
HpyCH4V TGCA 6 cut(s) 62, 144, 263, 317, 527, 584
HpyF10VI GCNNNNNNNGC 4 cut(s) 62, 308, 323, 613
HpyF3I CTNAG 1 cut(s) 542
HpySE526I ACGT 1 cut(s) 159
Hsp92I GRCGYC 1 cut(s) 744
Hsp92II CATG 5 cut(s) 62, 98, 113, 515, 797
HspAI GCGC 1 cut(s) 150
Ksp22I TGATCA 1 cut(s) 367
Kzo9I GATC 4 cut(s) 180, 367, 478, 827
LmnI GCTCC 2 cut(s) 299, 656
Lsp1109I GCAGC 3 cut(s) 74, 314, 814
LweI GCATC 2 cut(s) 250, 459
MaeI CTAG 3 cut(s) 53, 194, 618
MaeII ACGT 1 cut(s) 159
MaeIII GTNAC 3 cut(s) 47, 496, 568
MalI GATC 4 cut(s) 182, 369, 480, 829
MboI GATC 4 cut(s) 180, 367, 478, 827
MboII GAAGA 4 cut(s) 16, 19, 175, 851
MflI RGATCY 2 cut(s) 180, 827
MhlI GDGCHC 1 cut(s) 661
MluCI AATT 3 cut(s) 224, 424, 754
MlyI GAGTC 4 cut(s) 192, 565, 771, 799
MnlI CCTC 5 cut(s) 242, 591, 605, 647, 683
Mph1103I ATGCAT 1 cut(s) 265
MroXI GAANNNNTTC 1 cut(s) 333
MseI TTAA 1 cut(s) 837
MslI CAYNNNNRTG 1 cut(s) 375
MspI CCGG 1 cut(s) 45
MwoI GCNNNNNNNGC 4 cut(s) 62, 308, 323, 613
NdeI CATATG 1 cut(s) 292
NdeII GATC 4 cut(s) 180, 367, 478, 827
NlaIII CATG 5 cut(s) 62, 98, 113, 515, 797
NlaIV GGNNCC 1 cut(s) 658
NmuCI GTSAC 2 cut(s) 496, 568
NsiI ATGCAT 1 cut(s) 265
OliI CACNNNNGTG 1 cut(s) 375
PagI TCATGA 1 cut(s) 94
PdmI GAANNNNTTC 1 cut(s) 333
PfeI GAWTC 1 cut(s) 693
PflMI CCANNNNNTGG 1 cut(s) 296
PkrI GCNGC 3 cut(s) 64, 304, 804
PleI GAGTC 4 cut(s) 192, 565, 771, 798
PpsI GAGTC 4 cut(s) 192, 565, 771, 798
Ppu21I YACGTR 1 cut(s) 160
PspEI GGTNACC 1 cut(s) 47
PspN4I GGNNCC 1 cut(s) 658
PspPI GGNCC 3 cut(s) 209, 287, 614
PsuI RGATCY 2 cut(s) 180, 827
RsaI GTAC 3 cut(s) 114, 406, 445
RsaNI GTAC 3 cut(s) 113, 405, 444
RseI CAYNNNNRTG 1 cut(s) 375
SaqAI TTAA 1 cut(s) 837
SatI GCNGC 3 cut(s) 63, 303, 803
Sau3AI GATC 4 cut(s) 180, 367, 478, 827
Sau96I GGNCC 3 cut(s) 209, 287, 614
ScaI AGTACT 1 cut(s) 406
SchI GAGTC 4 cut(s) 192, 565, 771, 799
SduI GDGCHC 1 cut(s) 661
SfaNI GCATC 2 cut(s) 250, 459
SinI GGWCC 1 cut(s) 287
SmiMI CAYNNNNRTG 1 cut(s) 375
SmlI CTYRAG 2 cut(s) 306, 595
SmoI CTYRAG 2 cut(s) 306, 595
Sse9I AATT 3 cut(s) 224, 424, 754
SsiI CCGC 1 cut(s) 731
SspMI CTAG 3 cut(s) 53, 194, 618
StyI CCWWGG 1 cut(s) 748
TaaI ACNGT 5 cut(s) 249, 377, 502, 631, 713
TaiI ACGT 1 cut(s) 162
TaqI TCGA 2 cut(s) 138, 610
TasI AATT 3 cut(s) 224, 424, 754
TatI WGTACW 2 cut(s) 404, 443
TfiI GAWTC 1 cut(s) 693
Tru1I TTAA 1 cut(s) 837
Tru9I TTAA 1 cut(s) 837
TscAI CASTG 4 cut(s) 507, 586, 634, 718
TseFI GTSAC 2 cut(s) 496, 568
TseI GCWGC 3 cut(s) 62, 302, 802
Tsp45I GTSAC 2 cut(s) 496, 568
TspDTI ATGAA 4 cut(s) 17, 111, 500, 851
TspGWI ACGGA 1 cut(s) 332
TspRI CASTG 4 cut(s) 507, 586, 634, 718
Van91I CCANNNNNTGG 1 cut(s) 296
VpaK11BI GGWCC 1 cut(s) 287
XapI RAATTY 2 cut(s) 224, 754
XmiI GTMKAC 1 cut(s) 387
XmnI GAANNNNTTC 1 cut(s) 333
XspI CTAG 3 cut(s) 53, 194, 618
ZrmI AGTACT 1 cut(s) 406
Zsp2I ATGCAT 1 cut(s) 265
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.