Rw3G012510

strictosidine

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr3
OG0000674
Physical Location & Seq
Reverse (-)
12261340 .. 12262323
984 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw3G012510.1

Sequence Viewer

Length: 984 bp
ATGAGTGTGAGATCAAGTTTCGTGGTTTTCTCGTTTTTCTTTTTGGTTACAATAGCCTCATCTATTTCTCAAGCAGAAGCTCTTTCAATTGGTGGTTTTGAACACCATGAAATTCCTGTTCCAGTAGTTGGCCCTGAGAGTGTGGCGTTTGACTGCAGCGGAGCAGCCTACGTCGGGGTTGCGGATGGGTGGATTTTTAAATGGCTTGGGAGGCAATATGGATGGGCGGAGTTTGCCACAACCTCCCCATCTAGGCCAAGAGCATTGTGCGACGGCTCCACCAACAAAGACAATGAGCCAACTTGTGGGAGACCCCTCGGCCTCAAGTTCCACCCCACCACATGCGAGCTTTACATAGCCGATGCCTATTTCGGGCTCTTGAAGACAGGACCTAACGGCGGGGTAGCCCGACAACTTGCAACTTCAGCAGAAGGAATCCCTTTTCAGTTTACAAATTCTGTGGATATTAATCCCGTAACTGGAATGGTTTATTTCACAGATTCAAGCTCGGTTTATCAAAGAAGGAATTGGGAGTCGTCATTCAACACCGGTGACAAAACTGGAAGACTAATGCGATATAATCCAAGTGCAAACCGCGTGGAAGTTTTGCTCCGTGGTTTGTCTTTTGCAAATGGTGTAGCTGTAAGTCTAGATGGTAATTTCGTGCTGGTAACCCAAACAACAAGCAAAAACATTCTAAGGTATTGGGTTCGAGGACCAAGAGCTACTAATTCAGGTGTAGGAGAACTTTTTGCACGAACACAAGGCTTCCCTGACAATATTAACAGAAACATAAACGGAGAGTTTTGGGTTGCAGAGAATAGTAATGGAAGAGGTAAAGCAGCCAAGTTCGATGCCTACGGGAACTTCATGGAAGCCTTGGATGGGCTTAATCCTATTAGCCATGCCCAAGAATTTCATCATAATCTTTGGATAGGTTCGATTGTCAACCCCTTTGTGGGCGTTGTTCGCAACATTTATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Str_synth

Protein Analysis

327

Amino Acids

35.81

Weight (kDa)

7.01

Isoelectric Point (pI)

27.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Str_synth PF03088 152 - 239 2.3e-29 Strictosidine synthase
SGL PF08450 183 - 292 2.3e-09 SMP-30/Gluconolactonase/LRE-like region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 2 cut(s) 128, 305
AccII CGCG 1 cut(s) 597
AciI CCGC 5 cut(s) 159, 182, 227, 399, 595
AcsI RAATTY 3 cut(s) 111, 454, 914
AcuI CTGAAG 1 cut(s) 408
AgeI ACCGGT 1 cut(s) 548
AgsI TTSAA 5 cut(s) 87, 101, 382, 504, 544
AluBI AGCT 5 cut(s) 80, 349, 507, 641, 725
AluI AGCT 5 cut(s) 80, 349, 507, 641, 725
Alw26I GTCTC 1 cut(s) 304
AoxI GGCC 3 cut(s) 130, 254, 319
ApeKI GCWGC 3 cut(s) 156, 164, 842
ApoI RAATTY 3 cut(s) 111, 454, 914
AseI ATTAAT 1 cut(s) 468
AsiGI ACCGGT 1 cut(s) 548
AspS9I GGNCC 3 cut(s) 131, 389, 716
AsuHPI GGTGA 1 cut(s) 563
AvaII GGWCC 2 cut(s) 389, 716
BanII GRGCYC 1 cut(s) 378
BbsI GAAGAC 2 cut(s) 389, 571
BbvI GCAGC 3 cut(s) 168, 176, 854
BccI CCATC 5 cut(s) 179, 216, 256, 647, 878
BceAI ACGGC 2 cut(s) 289, 412
BcoDI GTCTC 1 cut(s) 304
BfaI CTAG 2 cut(s) 252, 650
BfmI CTRYAG 1 cut(s) 154
BisI GCNGC 3 cut(s) 157, 165, 843
BlsI GCNGC 3 cut(s) 158, 166, 844
Bme18I GGWCC 2 cut(s) 389, 716
BmgT120I GGNCC 3 cut(s) 131, 389, 716
BmiI GGNNCC 1 cut(s) 277
BmsI GCATC 2 cut(s) 352, 844
BpiI GAAGAC 2 cut(s) 389, 571
BpuEI CTTGAG 2 cut(s) 54, 308
BsaBI GATNNNNATC 1 cut(s) 468
BsaI GGTCTC 1 cut(s) 304
BsaJI CCNNGG 3 cut(s) 316, 613, 879
BsaWI WCCGGW 1 cut(s) 548
Bse118I RCCGGY 1 cut(s) 548
Bse1I ACTGG 3 cut(s) 122, 484, 565
Bse8I GATNNNNATC 1 cut(s) 468
BseDI CCNNGG 3 cut(s) 316, 613, 879
BseGI GGATG 3 cut(s) 190, 227, 889
BseJI GATNNNNATC 1 cut(s) 468
BseMII CTCAG 1 cut(s) 126
BseNI ACTGG 3 cut(s) 122, 484, 565
BseXI GCAGC 3 cut(s) 168, 176, 854
Bsh1236I CGCG 1 cut(s) 597
BshFI GGCC 3 cut(s) 132, 256, 321
BshTI ACCGGT 1 cut(s) 548
BsiSI CCGG 1 cut(s) 549
BsmAI GTCTC 1 cut(s) 304
BsnI GGCC 3 cut(s) 132, 256, 321
Bso31I GGTCTC 1 cut(s) 304
Bsp1286I GDGCHC 1 cut(s) 378
Bsp143I GATC 1 cut(s) 11
BspACI CCGC 5 cut(s) 159, 182, 227, 399, 595
BspANI GGCC 3 cut(s) 132, 256, 321
BspCNI CTCAG 1 cut(s) 127
BspFNI CGCG 1 cut(s) 597
BspLI GGNNCC 1 cut(s) 277
BspMAI CTGCAG 1 cut(s) 158
BspTNI GGTCTC 1 cut(s) 304
BsrFI RCCGGY 1 cut(s) 548
BsrI ACTGG 3 cut(s) 122, 484, 565
BssAI RCCGGY 1 cut(s) 548
BssECI CCNNGG 3 cut(s) 316, 613, 879
BssMI GATC 1 cut(s) 11
BssT1I CCWWGG 1 cut(s) 879
Bst6I CTCTTC 1 cut(s) 826
BstC8I GCNNGC 1 cut(s) 347
BstDEI CTNAG 2 cut(s) 135, 698
BstDSI CCRYGG 1 cut(s) 613
BstEII GGTNACC 1 cut(s) 670
BstF5I GGATG 3 cut(s) 190, 227, 889
BstFNI CGCG 1 cut(s) 597
BstKTI GATC 1 cut(s) 14
BstMAI GTCTC 1 cut(s) 304
BstMBI GATC 1 cut(s) 11
BstMWI GCNNNNNNNGC 4 cut(s) 211, 233, 425, 969
BstNSI RCATGY 1 cut(s) 345
BstPI GGTNACC 1 cut(s) 670
BstSFI CTRYAG 1 cut(s) 154
BstUI CGCG 1 cut(s) 597
BstV1I GCAGC 3 cut(s) 168, 176, 854
BstV2I GAAGAC 2 cut(s) 389, 571
BsuRI GGCC 3 cut(s) 132, 256, 321
BtgI CCRYGG 1 cut(s) 613
BtsCI GGATG 3 cut(s) 190, 227, 889
Cac8I GCNNGC 1 cut(s) 347
Cfr10I RCCGGY 1 cut(s) 548
Cfr13I GGNCC 3 cut(s) 131, 389, 716
CspAI ACCGGT 1 cut(s) 548
CspCI CAANNNNNGTGG 5 cut(s) 38, 441, 476, 579, 614
CviAII CATG 4 cut(s) 107, 342, 871, 905
DdeI CTNAG 2 cut(s) 135, 698
DpnI GATC 1 cut(s) 13
DpnII GATC 1 cut(s) 11
DraI TTTAAA 1 cut(s) 199
Eam1104I CTCTTC 1 cut(s) 826
EarI CTCTTC 1 cut(s) 826
EciI GGCGGA 1 cut(s) 242
Eco130I CCWWGG 1 cut(s) 879
Eco24I GRGCYC 1 cut(s) 378
Eco31I GGTCTC 1 cut(s) 304
Eco47I GGWCC 2 cut(s) 389, 716
Eco57I CTGAAG 1 cut(s) 408
Eco91I GGTNACC 1 cut(s) 670
EcoO109I RGGNCCY 1 cut(s) 389
EcoO65I GGTNACC 1 cut(s) 670
EcoT14I CCWWGG 1 cut(s) 879
EcoT38I GRGCYC 1 cut(s) 378
ErhI CCWWGG 1 cut(s) 879
FaeI CATG 4 cut(s) 110, 345, 874, 908
FaiI YATR 9 cut(s) 108, 219, 343, 356, 579, 794, 872, 906, 924
FatI CATG 4 cut(s) 106, 341, 870, 904
FauI CCCGC 1 cut(s) 392
Fnu4HI GCNGC 3 cut(s) 157, 165, 843
FokI GGATG 3 cut(s) 197, 234, 896
FriOI GRGCYC 1 cut(s) 378
Fsp4HI GCNGC 3 cut(s) 157, 165, 843
FspBI CTAG 2 cut(s) 252, 650
GluI GCNGC 3 cut(s) 157, 165, 843
HaeIII GGCC 3 cut(s) 132, 256, 321
HapII CCGG 1 cut(s) 549
Hin1II CATG 4 cut(s) 110, 345, 874, 908
HincII GTYRAC 1 cut(s) 949
HindII GTYRAC 1 cut(s) 949
HinfI GANTC 3 cut(s) 435, 500, 533
HpaII CCGG 1 cut(s) 549
HphI GGTGA 1 cut(s) 563
Hpy166II GTNNAC 2 cut(s) 450, 949
Hpy188III TCNNGA 2 cut(s) 379, 650
Hpy8I GTNNAC 2 cut(s) 450, 949
Hpy99I CGWCG 2 cut(s) 176, 275
HpyAV CCTTC 2 cut(s) 425, 516
HpyCH4IV ACGT 1 cut(s) 171
HpyCH4V TGCA 6 cut(s) 156, 419, 590, 629, 755, 815
HpyF10VI GCNNNNNNNGC 4 cut(s) 211, 233, 425, 969
HpyF3I CTNAG 2 cut(s) 135, 698
HpySE526I ACGT 1 cut(s) 171
Hsp92II CATG 4 cut(s) 110, 345, 874, 908
Kzo9I GATC 1 cut(s) 11
LmnI GCTCC 3 cut(s) 161, 281, 615
Lsp1109I GCAGC 3 cut(s) 168, 176, 854
LweI GCATC 2 cut(s) 352, 844
MaeI CTAG 2 cut(s) 252, 650
MaeII ACGT 1 cut(s) 171
MaeIII GTNAC 4 cut(s) 46, 475, 551, 670
MalI GATC 1 cut(s) 13
MboI GATC 1 cut(s) 11
MboII GAAGA 3 cut(s) 394, 576, 843
MfeI CAATTG 1 cut(s) 87
MhlI GDGCHC 1 cut(s) 378
MluCI AATT 7 cut(s) 87, 111, 454, 526, 658, 730, 914
MlyI GAGTC 1 cut(s) 542
MnlI CCTC 7 cut(s) 67, 204, 253, 326, 332, 707, 827
MseI TTAA 5 cut(s) 198, 468, 783, 891, 982
MspA1I CMGCKG 1 cut(s) 159
MspI CCGG 1 cut(s) 549
MunI CAATTG 1 cut(s) 87
MvnI CGCG 1 cut(s) 597
MwoI GCNNNNNNNGC 4 cut(s) 211, 233, 425, 969
NdeII GATC 1 cut(s) 11
NlaIII CATG 4 cut(s) 110, 345, 874, 908
NlaIV GGNNCC 1 cut(s) 277
NmeAIII GCCGAG 1 cut(s) 297
NmuCI GTSAC 1 cut(s) 551
NspI RCATGY 1 cut(s) 345
PfeI GAWTC 2 cut(s) 435, 500
PflMI CCANNNNNTGG 2 cut(s) 128, 305
PinAI ACCGGT 1 cut(s) 548
PkrI GCNGC 3 cut(s) 158, 166, 844
PleI GAGTC 1 cut(s) 541
PpsI GAGTC 1 cut(s) 541
PpuMI RGGWCCY 1 cut(s) 389
PshBI ATTAAT 1 cut(s) 468
Psp5II RGGWCCY 1 cut(s) 389
PspEI GGTNACC 1 cut(s) 670
PspN4I GGNNCC 1 cut(s) 277
PspPI GGNCC 3 cut(s) 131, 389, 716
PspPPI RGGWCCY 1 cut(s) 389
PstI CTGCAG 1 cut(s) 158
SaqAI TTAA 5 cut(s) 198, 468, 783, 891, 982
SatI GCNGC 3 cut(s) 157, 165, 843
Sau3AI GATC 1 cut(s) 11
Sau96I GGNCC 3 cut(s) 131, 389, 716
SchI GAGTC 1 cut(s) 542
SduI GDGCHC 1 cut(s) 378
SfaNI GCATC 2 cut(s) 352, 844
SfcI CTRYAG 1 cut(s) 154
SgrAI CRCCGGYG 1 cut(s) 548
SinI GGWCC 2 cut(s) 389, 716
SmlI CTYRAG 2 cut(s) 69, 323
SmoI CTYRAG 2 cut(s) 69, 323
Sse9I AATT 7 cut(s) 87, 111, 454, 526, 658, 730, 914
SsiI CCGC 5 cut(s) 159, 182, 227, 399, 595
SspI AATATT 1 cut(s) 781
SspMI CTAG 2 cut(s) 252, 650
StyI CCWWGG 1 cut(s) 879
TaiI ACGT 1 cut(s) 174
TaqI TCGA 3 cut(s) 712, 852, 941
TasI AATT 7 cut(s) 87, 111, 454, 526, 658, 730, 914
TfiI GAWTC 2 cut(s) 435, 500
Tru1I TTAA 5 cut(s) 198, 468, 783, 891, 982
Tru9I TTAA 5 cut(s) 198, 468, 783, 891, 982
TseFI GTSAC 1 cut(s) 551
TseI GCWGC 3 cut(s) 156, 164, 842
Tsp45I GTSAC 1 cut(s) 551
TspDTI ATGAA 3 cut(s) 123, 859, 908
TspGWI ACGGA 2 cut(s) 602, 813
Van91I CCANNNNNTGG 2 cut(s) 128, 305
VpaK11BI GGWCC 2 cut(s) 389, 716
VspI ATTAAT 1 cut(s) 468
XapI RAATTY 3 cut(s) 111, 454, 914
XbaI TCTAGA 1 cut(s) 649
XceI RCATGY 1 cut(s) 345
XspI CTAG 2 cut(s) 252, 650
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.