FvH4_7g02310

strictosidine

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0000674
Physical Location & Seq
Forward (+)
2639208 .. 2640182
975 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g02310.t1

Sequence Viewer

Length: 975 bp
ATGAAGTCAGCTCTTGTTATTGTCTTCTTGTTTTTCTTGTTTTTCTTTTTGTCTGCTAATAACCAAGCCCATGCAGCATCTTTTTCACTTCCATACCTAAACCATCATGAAATACCCATCCATGTACCACAGCCCGAAAGCATAGCTTTCGACTGCAATGGAGCACCCTACGTTGGTGTGGCTGATGGCAGGATATTCAAGTGGCTAGGAAGCCCGTTTGGGTGGATAGAATTTGCAGTCACCTCCGCCAATAGGCCTAGAGAAGTGTGCGATCTCTCAACCAGCAAAGACAATGAGCCGATATGCGGAAGGCCACTTGGTTTGAAATTTGACCTCACAACATGCGAACTGTATATTGCAGATGCCTATTTCGGACTTCTAAAGACAGGACCATATGGTGGAGTAGCCCAAGTACTTGCGACATCTGCTGAAGGGTTTCCTTTCCGTTTCTTGAATGGAGTGGACATTGATCACCAAAGTGGAATGGTCTACTTCACAGACACCAGTGCTATTTACCAGAGAAGGAATTGGGAATTGTCAGTTAGTACAGGTGATAAAACTGGAAGGTTAATGCGATACGATCCATCGACAAAGAAAGTGACAGTGTTGCTTCATGGCTTGGCTTTTGCAAACGGGGTGGCACTAAGCAAAGACAGTGATTTTGTTCTGGTAACCCAAACCACTGCAAAGAACGTCTTGAGGTATTGGCTTCAAGGGCCTAGAGCCAACACTGTTGATACTTTTTTCAACCTGCGAGGAGCCCCAGACAACATCAACATAAACATCAATGGAGAATTTTGGGTCGCCGAAAACAGTGCTGGAAGAGGTAAAGCGGTGAAGCTTGACGCCCAAGGAAATTTTCTTGACGTGGTAGATGGACTCAACCCTGTGAGTCACGTTGAGCAGCGTTACGGAAACCTTTGGATAGGCTCTGTGGTTAATAAGTTTGTCGGGGTTATTCTTAATTTTGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Str_synth

Protein Analysis

325

Amino Acids

35.76

Weight (kDa)

6.38

Isoelectric Point (pI)

22.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Str_synth PF03088 152 - 239 6e-31 Strictosidine synthase
SGL PF08450 183 - 292 1.9e-11 SMP-30/Gluconolactonase/LRE-like region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 759
AccB7I CCANNNNNTGG 1 cut(s) 398
AccI GTMKAC 1 cut(s) 489
AciI CCGC 3 cut(s) 246, 306, 833
AclWI GGATC 1 cut(s) 575
AcsI RAATTY 4 cut(s) 230, 326, 794, 856
AcuI CTGAAG 1 cut(s) 450
AcyI GRCGYC 1 cut(s) 846
AfaI GTAC 3 cut(s) 126, 414, 547
AfiI CCNNNNNNNGG 4 cut(s) 173, 252, 305, 398
AgsI TTSAA 5 cut(s) 199, 325, 454, 713, 748
AjiI CACGTC 1 cut(s) 868
AjuI GAANNNNNNNTTGG 2 cut(s) 843, 875
AleI CACNNNNGTG 1 cut(s) 477
AluBI AGCT 3 cut(s) 11, 146, 841
AluI AGCT 3 cut(s) 11, 146, 841
Alw21I GWGCWC 1 cut(s) 166
AlwI GGATC 1 cut(s) 575
AoxI GGCC 3 cut(s) 254, 311, 716
ApeKI GCWGC 2 cut(s) 74, 904
ApoI RAATTY 4 cut(s) 230, 326, 794, 856
ArsI GACNNNNNNTTYG 2 cut(s) 644, 676
Asp700I GAANNNNTTC 1 cut(s) 435
AspS9I GGNCC 2 cut(s) 389, 716
AsuHPI GGTGA 4 cut(s) 232, 464, 563, 847
AvaII GGWCC 1 cut(s) 389
BanII GRGCYC 1 cut(s) 763
BbsI GAAGAC 1 cut(s) 16
Bbv12I GWGCWC 1 cut(s) 166
BbvI GCAGC 2 cut(s) 86, 916
BccI CCATC 5 cut(s) 111, 125, 179, 592, 869
BcgI CGANNNNNNTGC 4 cut(s) 130, 164, 797, 831
BclI TGATCA 1 cut(s) 469
BfaI CTAG 3 cut(s) 206, 258, 720
BfuAI ACCTGC 1 cut(s) 759
BisI GCNGC 2 cut(s) 75, 905
BlsI GCNGC 2 cut(s) 76, 906
BmcAI AGTACT 1 cut(s) 414
Bme18I GGWCC 1 cut(s) 389
BmgBI CACGTC 1 cut(s) 868
BmgT120I GGNCC 2 cut(s) 389, 716
BmiI GGNNCC 1 cut(s) 760
BmsI GCATC 2 cut(s) 86, 352
BpiI GAAGAC 1 cut(s) 16
BpuEI CTTGAG 1 cut(s) 718
BsaHI GRCGYC 1 cut(s) 846
BsaJI CCNNGG 1 cut(s) 850
Bsc4I CCNNNNNNNGG 4 cut(s) 173, 252, 305, 398
Bse1I ACTGG 2 cut(s) 504, 565
Bse3DI GCAATG 1 cut(s) 163
BseDI CCNNGG 1 cut(s) 850
BseGI GGATG 1 cut(s) 117
BseLI CCNNNNNNNGG 4 cut(s) 173, 252, 305, 398
BseMI GCAATG 1 cut(s) 163
BseNI ACTGG 2 cut(s) 504, 565
BseRI GAGGAG 1 cut(s) 771
BseXI GCAGC 2 cut(s) 86, 916
BshFI GGCC 3 cut(s) 256, 313, 718
BsiHKAI GWGCWC 1 cut(s) 166
BslI CCNNNNNNNGG 4 cut(s) 173, 252, 305, 398
BsnI GGCC 3 cut(s) 256, 313, 718
Bsp1286I GDGCHC 2 cut(s) 166, 763
Bsp143I GATC 3 cut(s) 271, 469, 580
BspACI CCGC 3 cut(s) 246, 306, 833
BspANI GGCC 3 cut(s) 256, 313, 718
BspHI TCATGA 1 cut(s) 106
BspLI GGNNCC 1 cut(s) 760
BspMI ACCTGC 1 cut(s) 759
BspPI GGATC 1 cut(s) 575
BsrDI GCAATG 1 cut(s) 163
BsrI ACTGG 2 cut(s) 504, 565
BssECI CCNNGG 1 cut(s) 850
BssMI GATC 3 cut(s) 271, 469, 580
BssNI GRCGYC 1 cut(s) 846
BssT1I CCWWGG 1 cut(s) 850
Bst4CI ACNGT 5 cut(s) 351, 604, 656, 733, 815
Bst6I CTCTTC 1 cut(s) 817
BstACI GRCGYC 1 cut(s) 846
BstDEI CTNAG 1 cut(s) 644
BstEII GGTNACC 1 cut(s) 670
BstF5I GGATG 1 cut(s) 117
BstKTI GATC 3 cut(s) 274, 472, 583
BstMBI GATC 3 cut(s) 271, 469, 580
BstMWI GCNNNNNNNGC 3 cut(s) 74, 425, 715
BstNSI RCATGY 1 cut(s) 345
BstPI GGTNACC 1 cut(s) 670
BstV1I GCAGC 2 cut(s) 86, 916
BstV2I GAAGAC 1 cut(s) 16
BsuRI GGCC 3 cut(s) 256, 313, 718
BtrI CACGTC 1 cut(s) 868
BtsCI GGATG 1 cut(s) 117
BtsI GCAGTG 1 cut(s) 681
BtsIMutI CAGTG 6 cut(s) 511, 609, 661, 681, 729, 820
BveI ACCTGC 1 cut(s) 759
CciI TCATGA 1 cut(s) 106
Cfr13I GGNCC 2 cut(s) 389, 716
CseI GACGC 1 cut(s) 854
Csp6I GTAC 3 cut(s) 125, 413, 546
CspCI CAANNNNNGTGG 4 cut(s) 303, 338, 618, 653
CviAII CATG 5 cut(s) 71, 107, 122, 342, 614
CviQI GTAC 3 cut(s) 125, 413, 546
DdeI CTNAG 1 cut(s) 644
DpnI GATC 3 cut(s) 273, 471, 582
DpnII GATC 3 cut(s) 271, 469, 580
Eam1104I CTCTTC 1 cut(s) 817
EarI CTCTTC 1 cut(s) 817
EciI GGCGGA 1 cut(s) 235
Eco130I CCWWGG 1 cut(s) 850
Eco147I AGGCCT 1 cut(s) 256
Eco24I GRGCYC 1 cut(s) 763
Eco47I GGWCC 1 cut(s) 389
Eco57I CTGAAG 1 cut(s) 450
Eco91I GGTNACC 1 cut(s) 670
EcoO109I RGGNCCY 1 cut(s) 716
EcoO65I GGTNACC 1 cut(s) 670
EcoT14I CCWWGG 1 cut(s) 850
EcoT38I GRGCYC 1 cut(s) 763
ErhI CCWWGG 1 cut(s) 850
FaeI CATG 5 cut(s) 74, 110, 125, 345, 617
FalI AAGNNNNNCTT 4 cut(s) 130, 162, 680, 712
FatI CATG 5 cut(s) 70, 106, 121, 341, 613
FauNDI CATATG 1 cut(s) 394
FbaI TGATCA 1 cut(s) 469
FblI GTMKAC 1 cut(s) 489
Fnu4HI GCNGC 2 cut(s) 75, 905
FokI GGATG 1 cut(s) 104
FriOI GRGCYC 1 cut(s) 763
Fsp4HI GCNGC 2 cut(s) 75, 905
FspBI CTAG 3 cut(s) 206, 258, 720
GluI GCNGC 2 cut(s) 75, 905
HaeIII GGCC 3 cut(s) 256, 313, 718
HgaI GACGC 1 cut(s) 854
Hin1I GRCGYC 1 cut(s) 846
Hin1II CATG 5 cut(s) 74, 110, 125, 345, 617
HindIII AAGCTT 1 cut(s) 839
HinfI GANTC 2 cut(s) 879, 892
HphI GGTGA 4 cut(s) 232, 464, 563, 847
Hpy166II GTNNAC 2 cut(s) 463, 490
Hpy188I TCNGA 1 cut(s) 374
Hpy188III TCNNGA 4 cut(s) 107, 451, 697, 863
Hpy8I GTNNAC 2 cut(s) 463, 490
HpyAV CCTTC 4 cut(s) 303, 425, 516, 558
HpyCH4III ACNGT 5 cut(s) 351, 604, 656, 733, 815
HpyCH4IV ACGT 4 cut(s) 171, 693, 867, 897
HpyCH4V TGCA 6 cut(s) 74, 156, 236, 359, 629, 686
HpyF10VI GCNNNNNNNGC 3 cut(s) 74, 425, 715
HpyF3I CTNAG 1 cut(s) 644
HpySE526I ACGT 4 cut(s) 171, 693, 867, 897
Hsp92I GRCGYC 1 cut(s) 846
Hsp92II CATG 5 cut(s) 74, 110, 125, 345, 617
Ksp22I TGATCA 1 cut(s) 469
Kzo9I GATC 3 cut(s) 271, 469, 580
LmnI GCTCC 2 cut(s) 161, 758
Lsp1109I GCAGC 2 cut(s) 86, 916
LweI GCATC 2 cut(s) 86, 352
MaeI CTAG 3 cut(s) 206, 258, 720
MaeII ACGT 4 cut(s) 171, 693, 867, 897
MaeIII GTNAC 5 cut(s) 238, 598, 670, 893, 908
MalI GATC 3 cut(s) 273, 471, 582
MboI GATC 3 cut(s) 271, 469, 580
MboII GAAGA 2 cut(s) 16, 834
MhlI GDGCHC 2 cut(s) 166, 763
MluCI AATT 7 cut(s) 230, 326, 526, 533, 794, 856, 964
MlyI GAGTC 2 cut(s) 873, 901
MnlI CCTC 5 cut(s) 253, 344, 693, 749, 818
MroXI GAANNNNTTC 1 cut(s) 435
MseI TTAA 3 cut(s) 569, 939, 963
MslI CAYNNNNRTG 1 cut(s) 477
MwoI GCNNNNNNNGC 3 cut(s) 74, 425, 715
NdeI CATATG 1 cut(s) 394
NdeII GATC 3 cut(s) 271, 469, 580
NlaIII CATG 5 cut(s) 74, 110, 125, 345, 617
NlaIV GGNNCC 1 cut(s) 760
NmuCI GTSAC 3 cut(s) 238, 598, 893
NspI RCATGY 1 cut(s) 345
OliI CACNNNNGTG 1 cut(s) 477
PagI TCATGA 1 cut(s) 106
PceI AGGCCT 1 cut(s) 256
PdmI GAANNNNTTC 1 cut(s) 435
PflMI CCANNNNNTGG 1 cut(s) 398
PkrI GCNGC 2 cut(s) 76, 906
PleI GAGTC 2 cut(s) 873, 900
PpsI GAGTC 2 cut(s) 873, 900
PspEI GGTNACC 1 cut(s) 670
PspN4I GGNNCC 1 cut(s) 760
PspPI GGNCC 2 cut(s) 389, 716
RsaI GTAC 3 cut(s) 126, 414, 547
RsaNI GTAC 3 cut(s) 125, 413, 546
RseI CAYNNNNRTG 1 cut(s) 477
SaqAI TTAA 3 cut(s) 569, 939, 963
SatI GCNGC 2 cut(s) 75, 905
Sau3AI GATC 3 cut(s) 271, 469, 580
Sau96I GGNCC 2 cut(s) 389, 716
ScaI AGTACT 1 cut(s) 414
SchI GAGTC 2 cut(s) 873, 901
SduI GDGCHC 2 cut(s) 166, 763
SfaNI GCATC 2 cut(s) 86, 352
SinI GGWCC 1 cut(s) 389
SmiMI CAYNNNNRTG 1 cut(s) 477
SmlI CTYRAG 1 cut(s) 697
SmoI CTYRAG 1 cut(s) 697
Sse9I AATT 7 cut(s) 230, 326, 526, 533, 794, 856, 964
SseBI AGGCCT 1 cut(s) 256
SsiI CCGC 3 cut(s) 246, 306, 833
SspMI CTAG 3 cut(s) 206, 258, 720
StuI AGGCCT 1 cut(s) 256
StyI CCWWGG 1 cut(s) 850
TaaI ACNGT 5 cut(s) 351, 604, 656, 733, 815
TaiI ACGT 4 cut(s) 174, 696, 870, 900
TaqI TCGA 2 cut(s) 150, 587
TasI AATT 7 cut(s) 230, 326, 526, 533, 794, 856, 964
TatI WGTACW 2 cut(s) 412, 545
Tru1I TTAA 3 cut(s) 569, 939, 963
Tru9I TTAA 3 cut(s) 569, 939, 963
TscAI CASTG 6 cut(s) 511, 609, 661, 688, 736, 820
TseFI GTSAC 3 cut(s) 238, 598, 893
TseI GCWGC 2 cut(s) 74, 904
Tsp45I GTSAC 3 cut(s) 238, 598, 893
TspDTI ATGAA 3 cut(s) 17, 123, 602
TspGWI ACGGA 2 cut(s) 434, 927
TspRI CASTG 6 cut(s) 511, 609, 661, 688, 736, 820
Van91I CCANNNNNTGG 1 cut(s) 398
VpaK11BI GGWCC 1 cut(s) 389
XapI RAATTY 4 cut(s) 230, 326, 794, 856
XceI RCATGY 1 cut(s) 345
XmiI GTMKAC 1 cut(s) 489
XmnI GAANNNNTTC 1 cut(s) 435
XspI CTAG 3 cut(s) 206, 258, 720
ZrmI AGTACT 1 cut(s) 414
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.