Rw1G002530

strictosidine

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0000674
Physical Location & Seq
Forward (+)
4571389 .. 4572348
960 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G002530.1

Sequence Viewer

Length: 960 bp
ATGAAATCAGCTCTTGTTATTCTCTTCTTCTTCTGTTTGTCTGTTCACCAATCCCAAGGAGCATCTTTTTCACTCCCAAACCTAAACCATCATGAAATACCCATCCACGTACCACAGCCCGAAAGCATTGCTTTCGACTGCAATGGCGCTCCCTACGTTGGTGTGGCTGATGGCAGGATATTCAAGTGGCTAGGATACCCTTTTGGGTGGAGAGAATTCGCAGTCACCTCCCCCAATAGGCCGAGAGGAGTATGTGATTTAACAACCAACAAAGACAATGAACCGATATGCGGGAGGCCACTTGGTTTGAAATTTGACCTCACAACATGCGAACTGTATATCGCAGATGCCTATTTTGGGCTTCTAAAGACAGGACCATATGGTGGAGTAGCCCAAATACTTGCGACATCTGCTGAAGGAGTTCCTTTCCGTTTCTTGAATGGTTTGGACATTGATCGCCAAAGTGGAATGGTGTACTTCACAGACACCAGTGCTATTTACCAGAGACGAAATTGGGAATTGTCAGTTAGTACAGGTGATAAAACTGGAAGGTTAATGCGATATGATCCATGGACAAAGAATGTCACTGTGTTGCGTAATGACTTGTCCTTTGCAAATGGGGTGGCACTAAGCAAAGACGGTGATTTTGTGCTAGTAACCCAAACCACCGCAAAGAACATTTTGAGGTACTGGCTTCGAGGGCCTAGAGCCAATACTGTTGATATTTTTTTCCAACTGCAAGGAGCCCCAGACAATATCATCCGAAACATAAATGGAGAATTTTGGGTTGCGGAAAATAGTGCCGGAAAAGGTAAAGCGGTCAGGCTTGACGCTCAAGGAAATTTTCTGGACGTGGTAGATGGACTCAACCCGGTGAGTCATGTTGAGCAGCGCTATAGAAATCTTTGGATAGGATCTGTGGTTAATAACTTTGTTGGGGTTATTCTTAATTTTGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Str_synth

Protein Analysis

319

Amino Acids

35.52

Weight (kDa)

7.64

Isoelectric Point (pI)

27.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Str_synth PF03088 147 - 234 3.9e-30 Strictosidine synthase
SGL PF08450 178 - 287 3.7e-11 SMP-30/Gluconolactonase/LRE-like region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 383
AciI CCGC 4 cut(s) 291, 669, 791, 818
AclWI GGATC 2 cut(s) 560, 922
AcsI RAATTY 4 cut(s) 215, 311, 779, 841
AcuI CTGAAG 1 cut(s) 435
AfaI GTAC 4 cut(s) 111, 476, 532, 689
AfeI AGCGCT 1 cut(s) 893
AfiI CCNNNNNNNGG 5 cut(s) 158, 237, 290, 357, 383
AgsI TTSAA 3 cut(s) 184, 310, 439
AjiI CACGTC 1 cut(s) 853
AluBI AGCT 1 cut(s) 11
AluI AGCT 1 cut(s) 11
Alw26I GTCTC 1 cut(s) 499
AlwI GGATC 2 cut(s) 560, 922
Aor51HI AGCGCT 1 cut(s) 893
AoxI GGCC 3 cut(s) 239, 296, 701
ApeKI GCWGC 1 cut(s) 889
ApoI RAATTY 4 cut(s) 215, 311, 779, 841
ArsI GACNNNNNNTTYG 4 cut(s) 593, 625, 629, 661
Asp700I GAANNNNTTC 1 cut(s) 420
AspLEI GCGC 2 cut(s) 149, 894
AspS9I GGNCC 2 cut(s) 374, 701
AsuC2I CCSGG 1 cut(s) 872
AsuHPI GGTGA 5 cut(s) 38, 217, 548, 653, 886
AvaII GGWCC 1 cut(s) 374
BanII GRGCYC 1 cut(s) 748
BbvI GCAGC 1 cut(s) 901
BccI CCATC 4 cut(s) 96, 110, 164, 854
BcgI CGANNNNNNTGC 4 cut(s) 110, 115, 144, 149
BciVI GTATCC 1 cut(s) 188
BcnI CCSGG 1 cut(s) 872
BcoDI GTCTC 1 cut(s) 499
BfaI CTAG 3 cut(s) 191, 653, 705
BfmI CTRYAG 1 cut(s) 895
BfoI RGCGCY 2 cut(s) 150, 895
BfuI GTATCC 1 cut(s) 188
BisI GCNGC 1 cut(s) 890
BlsI GCNGC 1 cut(s) 891
Bme1390I CCNGG 1 cut(s) 872
Bme18I GGWCC 1 cut(s) 374
BmgBI CACGTC 1 cut(s) 853
BmgT120I GGNCC 2 cut(s) 374, 701
BmiI GGNNCC 1 cut(s) 745
BmrFI CCNGG 1 cut(s) 872
BmsI GCATC 2 cut(s) 71, 337
BpuEI CTTGAG 1 cut(s) 819
BpuMI CCSGG 1 cut(s) 872
BsaAI YACGTR 1 cut(s) 109
BsaJI CCNNGG 2 cut(s) 55, 569
Bsc4I CCNNNNNNNGG 5 cut(s) 158, 237, 290, 357, 383
Bse1I ACTGG 3 cut(s) 489, 550, 695
Bse3DI GCAATG 2 cut(s) 126, 148
BseDI CCNNGG 2 cut(s) 55, 569
BseGI GGATG 2 cut(s) 102, 759
BseLI CCNNNNNNNGG 5 cut(s) 158, 237, 290, 357, 383
BseMI GCAATG 2 cut(s) 126, 148
BseNI ACTGG 3 cut(s) 489, 550, 695
BseRI GAGGAG 1 cut(s) 261
BseXI GCAGC 1 cut(s) 901
BshFI GGCC 3 cut(s) 241, 298, 703
BsiSI CCGG 2 cut(s) 804, 872
BslI CCNNNNNNNGG 5 cut(s) 158, 237, 290, 357, 383
BsmAI GTCTC 1 cut(s) 499
BsmBI CGTCTC 1 cut(s) 499
BsnI GGCC 3 cut(s) 241, 298, 703
Bsp1286I GDGCHC 1 cut(s) 748
Bsp143I GATC 3 cut(s) 454, 565, 914
Bsp19I CCATGG 1 cut(s) 569
BspACI CCGC 4 cut(s) 291, 669, 791, 818
BspANI GGCC 3 cut(s) 241, 298, 703
BspHI TCATGA 1 cut(s) 91
BspLI GGNNCC 1 cut(s) 745
BspPI GGATC 2 cut(s) 560, 922
BsrDI GCAATG 2 cut(s) 126, 148
BsrI ACTGG 3 cut(s) 489, 550, 695
BssECI CCNNGG 2 cut(s) 55, 569
BssMI GATC 3 cut(s) 454, 565, 914
BssT1I CCWWGG 2 cut(s) 55, 569
Bst4CI ACNGT 4 cut(s) 336, 589, 641, 718
Bst6I CTCTTC 1 cut(s) 29
BstBAI YACGTR 1 cut(s) 109
BstDEI CTNAG 1 cut(s) 629
BstDSI CCRYGG 1 cut(s) 569
BstF5I GGATG 2 cut(s) 102, 759
BstH2I RGCGCY 2 cut(s) 150, 895
BstHHI GCGC 2 cut(s) 149, 894
BstKTI GATC 3 cut(s) 457, 568, 917
BstMAI GTCTC 1 cut(s) 499
BstMBI GATC 3 cut(s) 454, 565, 914
BstMWI GCNNNNNNNGC 2 cut(s) 410, 700
BstNSI RCATGY 1 cut(s) 330
BstSCI CCNGG 1 cut(s) 870
BstSFI CTRYAG 1 cut(s) 895
BstV1I GCAGC 1 cut(s) 901
BstX2I RGATCY 1 cut(s) 914
BstYI RGATCY 1 cut(s) 914
BsuI GTATCC 1 cut(s) 188
BsuRI GGCC 3 cut(s) 241, 298, 703
BtgI CCRYGG 1 cut(s) 569
BtrI CACGTC 1 cut(s) 853
BtsCI GGATG 2 cut(s) 102, 759
BtsIMutI CAGTG 2 cut(s) 496, 585
CciI TCATGA 1 cut(s) 91
CfoI GCGC 2 cut(s) 149, 894
Cfr13I GGNCC 2 cut(s) 374, 701
CseI GACGC 1 cut(s) 839
Csp6I GTAC 4 cut(s) 110, 475, 531, 688
CspCI CAANNNNNGTGG 4 cut(s) 288, 323, 603, 638
CviAII CATG 4 cut(s) 92, 327, 570, 881
CviQI GTAC 4 cut(s) 110, 475, 531, 688
DdeI CTNAG 1 cut(s) 629
DpnI GATC 3 cut(s) 456, 567, 916
DpnII GATC 3 cut(s) 454, 565, 914
Eam1104I CTCTTC 1 cut(s) 29
EarI CTCTTC 1 cut(s) 29
Eco130I CCWWGG 2 cut(s) 55, 569
Eco24I GRGCYC 1 cut(s) 748
Eco47I GGWCC 1 cut(s) 374
Eco47III AGCGCT 1 cut(s) 893
Eco57I CTGAAG 1 cut(s) 435
EcoO109I RGGNCCY 1 cut(s) 701
EcoRI GAATTC 1 cut(s) 215
EcoT14I CCWWGG 2 cut(s) 55, 569
EcoT38I GRGCYC 1 cut(s) 748
ErhI CCWWGG 2 cut(s) 55, 569
Esp3I CGTCTC 1 cut(s) 499
FaeI CATG 4 cut(s) 95, 330, 573, 884
FalI AAGNNNNNCTT 2 cut(s) 115, 147
FatI CATG 4 cut(s) 91, 326, 569, 880
FauI CCCGC 1 cut(s) 284
FauNDI CATATG 1 cut(s) 379
Fnu4HI GCNGC 1 cut(s) 890
FokI GGATG 2 cut(s) 89, 746
FriOI GRGCYC 1 cut(s) 748
Fsp4HI GCNGC 1 cut(s) 890
FspBI CTAG 3 cut(s) 191, 653, 705
GlaI GCGC 2 cut(s) 148, 893
GluI GCNGC 1 cut(s) 890
HaeII RGCGCY 2 cut(s) 150, 895
HaeIII GGCC 3 cut(s) 241, 298, 703
HapII CCGG 2 cut(s) 804, 872
HgaI GACGC 1 cut(s) 839
HhaI GCGC 2 cut(s) 149, 894
Hin1II CATG 4 cut(s) 95, 330, 573, 884
Hin6I GCGC 2 cut(s) 147, 892
HinP1I GCGC 2 cut(s) 147, 892
HinfI GANTC 2 cut(s) 864, 877
HpaII CCGG 2 cut(s) 804, 872
HphI GGTGA 5 cut(s) 38, 217, 548, 653, 886
Hpy166II GTNNAC 2 cut(s) 46, 475
Hpy188I TCNGA 1 cut(s) 764
Hpy188III TCNNGA 3 cut(s) 92, 436, 848
Hpy8I GTNNAC 2 cut(s) 46, 475
HpyAV CCTTC 2 cut(s) 410, 543
HpyCH4III ACNGT 4 cut(s) 336, 589, 641, 718
HpyCH4IV ACGT 3 cut(s) 108, 156, 852
HpyCH4V TGCA 3 cut(s) 141, 614, 739
HpyF10VI GCNNNNNNNGC 2 cut(s) 410, 700
HpyF3I CTNAG 1 cut(s) 629
HpySE526I ACGT 3 cut(s) 108, 156, 852
Hsp92II CATG 4 cut(s) 95, 330, 573, 884
HspAI GCGC 2 cut(s) 147, 892
Kzo9I GATC 3 cut(s) 454, 565, 914
LmnI GCTCC 3 cut(s) 59, 154, 743
Lsp1109I GCAGC 1 cut(s) 901
LweI GCATC 2 cut(s) 71, 337
MaeI CTAG 3 cut(s) 191, 653, 705
MaeII ACGT 3 cut(s) 108, 156, 852
MaeIII GTNAC 3 cut(s) 223, 583, 655
MalI GATC 3 cut(s) 456, 567, 916
MboI GATC 3 cut(s) 454, 565, 914
MboII GAAGA 3 cut(s) 16, 19, 22
MflI RGATCY 1 cut(s) 914
MhlI GDGCHC 1 cut(s) 748
MluCI AATT 7 cut(s) 215, 311, 511, 518, 779, 841, 949
MlyI GAGTC 2 cut(s) 858, 886
MmeI TCCRAC 1 cut(s) 757
MnlI CCTC 6 cut(s) 238, 239, 288, 329, 678, 692
MroXI GAANNNNTTC 1 cut(s) 420
MseI TTAA 4 cut(s) 260, 554, 924, 948
MspI CCGG 2 cut(s) 804, 872
MspR9I CCNGG 1 cut(s) 872
MwoI GCNNNNNNNGC 2 cut(s) 410, 700
NciI CCSGG 1 cut(s) 872
NcoI CCATGG 1 cut(s) 569
NdeI CATATG 1 cut(s) 379
NdeII GATC 3 cut(s) 454, 565, 914
NlaIII CATG 4 cut(s) 95, 330, 573, 884
NlaIV GGNNCC 1 cut(s) 745
NmeAIII GCCGAG 1 cut(s) 267
NmuCI GTSAC 2 cut(s) 223, 583
NspI RCATGY 1 cut(s) 330
PagI TCATGA 1 cut(s) 91
PdmI GAANNNNTTC 1 cut(s) 420
PflMI CCANNNNNTGG 1 cut(s) 383
PkrI GCNGC 1 cut(s) 891
PleI GAGTC 2 cut(s) 858, 885
PpsI GAGTC 2 cut(s) 858, 885
Ppu21I YACGTR 1 cut(s) 109
PspN4I GGNNCC 1 cut(s) 745
PspPI GGNCC 2 cut(s) 374, 701
PsuI RGATCY 1 cut(s) 914
RsaI GTAC 4 cut(s) 111, 476, 532, 689
RsaNI GTAC 4 cut(s) 110, 475, 531, 688
SaqAI TTAA 4 cut(s) 260, 554, 924, 948
SatI GCNGC 1 cut(s) 890
Sau3AI GATC 3 cut(s) 454, 565, 914
Sau96I GGNCC 2 cut(s) 374, 701
SchI GAGTC 2 cut(s) 858, 886
ScrFI CCNGG 1 cut(s) 872
SduI GDGCHC 1 cut(s) 748
SfaNI GCATC 2 cut(s) 71, 337
SfcI CTRYAG 1 cut(s) 895
SinI GGWCC 1 cut(s) 374
SmlI CTYRAG 1 cut(s) 834
SmoI CTYRAG 1 cut(s) 834
Sse9I AATT 7 cut(s) 215, 311, 511, 518, 779, 841, 949
SsiI CCGC 4 cut(s) 291, 669, 791, 818
SspMI CTAG 3 cut(s) 191, 653, 705
StyD4I CCNGG 1 cut(s) 870
StyI CCWWGG 2 cut(s) 55, 569
TaaI ACNGT 4 cut(s) 336, 589, 641, 718
TaiI ACGT 3 cut(s) 111, 159, 855
TaqI TCGA 2 cut(s) 135, 697
TasI AATT 7 cut(s) 215, 311, 511, 518, 779, 841, 949
TatI WGTACW 2 cut(s) 474, 530
Tru1I TTAA 4 cut(s) 260, 554, 924, 948
Tru9I TTAA 4 cut(s) 260, 554, 924, 948
TscAI CASTG 2 cut(s) 496, 592
TseFI GTSAC 2 cut(s) 223, 583
TseI GCWGC 1 cut(s) 889
Tsp45I GTSAC 2 cut(s) 223, 583
TspDTI ATGAA 3 cut(s) 17, 108, 294
TspGWI ACGGA 1 cut(s) 419
TspRI CASTG 2 cut(s) 496, 592
Van91I CCANNNNNTGG 1 cut(s) 383
VpaK11BI GGWCC 1 cut(s) 374
XapI RAATTY 4 cut(s) 215, 311, 779, 841
XceI RCATGY 1 cut(s) 330
XmnI GAANNNNTTC 1 cut(s) 420
XspI CTAG 3 cut(s) 191, 653, 705
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.