RchiOBHm_Chr4g0436721

strictosidine

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0000674
Physical Location & Seq
Reverse (-)
59960283 .. 59965421
5139 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ40504

Sequence Viewer

Length: 1044 bp
ATGAACCCTCCTCCTTTTGCTTCATTCTACTTGCTCCTGTCTTGTCTACTGATCATCCTAGTATCAACCTCGACATCCAAACCAGCATTAGCTTTCTCCATTGCTAGCCTCAGGAAATACCACCAACTCGAGCTCCCTTATAACGTAGTCGGCCCCGAAAGCGTTGCGTTCGACTGCCGTGGAGAAGGACCTTATGTTGGGGTTTCAGATGGTAGAATTCTCAAATGGGAGGAACCTCACAACGGCTGGAGAGAGTTTGGCTACACTTCCCCGAATAGGCAGAGGAAATTATGTGATGGCTCAACCGACAAGATAAATGAACCGATATGCGGAAGGCCACTGGGTCTAAAGTTTAACCCTACAACATGTGAACTGTATATAGCAGATGCCTATTTTGGACTCTTGAAGACGGGACCAAATGGTGGCCGTCCTCATCAACTCGCCACTTCTGCAGGAGGAGTCCCCTTCCATTTCTTAAATGCTTTGGACATCGATGACGAATCTGGAATGGTTTATTTTACGGACACTAGCATTAATTTCCAAAGAAGTGTTTGGATATTGTCCATCATCTCCGGTGACAGAACTGGAAGGTTAATGCAATACGACCCTAGTACAAAGAAGGTGACTGTGTTGCTCAAGGGATTGGCATTTCCAAATGGTGTGGCTCTAACTAAGGACAAATCTTTCCTCCTCCTAGCCGAAACTGGGACGCTGAAAATCCTCCGGTTGTGGCTTCGAGGACCCAAATCTAATGCCTTGGAACTTTTTGCTCAACTAGTCGGGTTCCCAGATAACATCAAGAGAAACAACAATGGAGAATTCTGGGTTGCGATCAACAACGGAAGAGGAAGATCAATATCTCGGACTCTGCTAGGAGATCCTGTAGGAGCAAAGTTTGATGAAGAAGGAAACGTTTTGGAGGTGTTAGATGGAGAGGATAGTACGGCAGCGTTGCTTCAGTCTGTTAGTGAAGTTGAAGAACACAACGGAAAGTTGTGGATGGGATCGGTGGAAAATTCATATGTTGGTGTTGGCTTGTTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Str_synth

Protein Analysis

347

Amino Acids

38.13

Weight (kDa)

6.02

Isoelectric Point (pI)

32.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Str_synth PF03088 160 - 247 1.8e-29 Strictosidine synthase
SGL PF08450 166 - 282 1.7e-08 SMP-30/Gluconolactonase/LRE-like region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 141
AccB7I CCANNNNNTGG 1 cut(s) 422
AccI GTMKAC 1 cut(s) 46
AciI CCGC 1 cut(s) 330
AclI AACGTT 1 cut(s) 912
AclWI GGATC 2 cut(s) 872, 1012
AcoI YGGCCR 1 cut(s) 424
AcsI RAATTY 3 cut(s) 216, 818, 1015
AcuI CTGAAG 1 cut(s) 941
AfaI GTAC 2 cut(s) 613, 943
AfiI CCNNNNNNNGG 6 cut(s) 197, 242, 276, 329, 422, 705
AflIII ACRYGT 1 cut(s) 365
AgsI TTSAA 2 cut(s) 406, 977
AhlI ACTAGT 1 cut(s) 775
AluBI AGCT 2 cut(s) 92, 133
AluI AGCT 2 cut(s) 92, 133
Alw21I GWGCWC 1 cut(s) 135
AlwI GGATC 2 cut(s) 872, 1012
Ama87I CYCGRG 1 cut(s) 128
AoxI GGCC 3 cut(s) 151, 335, 424
ApeKI GCWGC 1 cut(s) 947
ApoI RAATTY 3 cut(s) 216, 818, 1015
AseI ATTAAT 1 cut(s) 534
AspS9I GGNCC 4 cut(s) 152, 188, 413, 740
AsuHPI GGTGA 2 cut(s) 587, 634
AsuNHI GCTAGC 1 cut(s) 104
AvaI CYCGRG 1 cut(s) 128
AvaII GGWCC 3 cut(s) 188, 413, 740
AxyI CCTNAGG 1 cut(s) 110
BanII GRGCYC 1 cut(s) 135
BbsI GAAGAC 1 cut(s) 413
Bbv12I GWGCWC 1 cut(s) 135
BbvI GCAGC 1 cut(s) 959
BccI CCATC 5 cut(s) 203, 290, 572, 923, 994
BceAI ACGGC 4 cut(s) 162, 259, 411, 960
BcgI CGANNNNNNTGC 2 cut(s) 146, 180
BclI TGATCA 1 cut(s) 51
BcuI ACTAGT 1 cut(s) 775
BfaI CTAG 7 cut(s) 59, 105, 528, 609, 695, 776, 872
BfmI CTRYAG 2 cut(s) 450, 882
BisI GCNGC 1 cut(s) 948
BlsI GCNGC 1 cut(s) 949
Bme18I GGWCC 3 cut(s) 188, 413, 740
BmeT110I CYCGRG 1 cut(s) 128
BmgT120I GGNCC 4 cut(s) 152, 188, 413, 740
BmiI GGNNCC 5 cut(s) 154, 234, 414, 742, 785
BmrI ACTGGG 2 cut(s) 350, 714
BmsI GCATC 1 cut(s) 376
BmtI GCTAGC 1 cut(s) 108
BmuI ACTGGG 2 cut(s) 350, 714
BpiI GAAGAC 1 cut(s) 413
BpmI CTGGAG 1 cut(s) 268
BpuEI CTTGAG 1 cut(s) 620
Bsa29I ATCGAT 1 cut(s) 492
BsaBI GATNNNNATC 1 cut(s) 856
BsaJI CCNNGG 2 cut(s) 178, 756
BsaWI WCCGGW 2 cut(s) 572, 723
BsaXI ACNNNNNCTCC 4 cut(s) 117, 147, 867, 897
Bsc4I CCNNNNNNNGG 6 cut(s) 197, 242, 276, 329, 422, 705
Bse1I ACTGG 3 cut(s) 345, 589, 709
Bse21I CCTNAGG 1 cut(s) 110
Bse3DI GCAATG 1 cut(s) 99
Bse8I GATNNNNATC 1 cut(s) 856
BseCI ATCGAT 1 cut(s) 492
BseDI CCNNGG 2 cut(s) 178, 756
BseGI GGATG 3 cut(s) 54, 74, 1005
BseJI GATNNNNATC 1 cut(s) 856
BseLI CCNNNNNNNGG 6 cut(s) 197, 242, 276, 329, 422, 705
BseMI GCAATG 1 cut(s) 99
BseMII CTCAG 1 cut(s) 124
BseNI ACTGG 3 cut(s) 345, 589, 709
BseRI GAGGAG 2 cut(s) 471, 680
BseXI GCAGC 1 cut(s) 959
BshFI GGCC 3 cut(s) 153, 337, 426
BshVI ATCGAT 1 cut(s) 492
BsiHKAI GWGCWC 1 cut(s) 135
BsiHKCI CYCGRG 1 cut(s) 128
BsiSI CCGG 2 cut(s) 573, 724
BslFI GGGAC 3 cut(s) 426, 446, 721
BslI CCNNNNNNNGG 6 cut(s) 197, 242, 276, 329, 422, 705
BsmFI GGGAC 3 cut(s) 426, 446, 721
BsnI GGCC 3 cut(s) 153, 337, 426
BsoBI CYCGRG 1 cut(s) 128
Bsp1286I GDGCHC 1 cut(s) 135
Bsp143I GATC 5 cut(s) 51, 831, 851, 877, 1004
BspACI CCGC 1 cut(s) 330
BspANI GGCC 3 cut(s) 153, 337, 426
BspCNI CTCAG 1 cut(s) 123
BspDI ATCGAT 1 cut(s) 492
BspLI GGNNCC 5 cut(s) 154, 234, 414, 742, 785
BspMAI CTGCAG 1 cut(s) 454
BspOI GCTAGC 1 cut(s) 108
BspPI GGATC 2 cut(s) 872, 1012
BsrDI GCAATG 1 cut(s) 99
BsrI ACTGG 3 cut(s) 345, 589, 709
BssECI CCNNGG 2 cut(s) 178, 756
BssMI GATC 5 cut(s) 51, 831, 851, 877, 1004
BssT1I CCWWGG 1 cut(s) 756
Bst4CI ACNGT 2 cut(s) 375, 628
Bst6I CTCTTC 1 cut(s) 838
BstC8I GCNNGC 1 cut(s) 106
BstDEI CTNAG 2 cut(s) 110, 672
BstDSI CCRYGG 1 cut(s) 178
BstF5I GGATG 3 cut(s) 54, 74, 1005
BstKTI GATC 5 cut(s) 54, 834, 854, 880, 1007
BstMBI GATC 5 cut(s) 51, 831, 851, 877, 1004
BstMWI GCNNNNNNNGC 2 cut(s) 159, 449
BstNSI RCATGY 1 cut(s) 369
BstSFI CTRYAG 2 cut(s) 450, 882
BstV1I GCAGC 1 cut(s) 959
BstV2I GAAGAC 1 cut(s) 413
BstX2I RGATCY 1 cut(s) 877
BstYI RGATCY 1 cut(s) 877
Bsu15I ATCGAT 1 cut(s) 492
Bsu36I CCTNAGG 1 cut(s) 110
BsuRI GGCC 3 cut(s) 153, 337, 426
BsuTUI ATCGAT 1 cut(s) 492
BtgI CCRYGG 1 cut(s) 178
BtsCI GGATG 3 cut(s) 54, 74, 1005
BtsIMutI CAGTG 1 cut(s) 338
Cac8I GCNNGC 1 cut(s) 106
Cfr13I GGNCC 4 cut(s) 152, 188, 413, 740
ClaI ATCGAT 1 cut(s) 492
CseI GACGC 1 cut(s) 718
Csp6I GTAC 2 cut(s) 612, 942
CspCI CAANNNNNGTGG 2 cut(s) 642, 677
CviAII CATG 1 cut(s) 366
CviQI GTAC 2 cut(s) 612, 942
DdeI CTNAG 2 cut(s) 110, 672
DpnI GATC 5 cut(s) 53, 833, 853, 879, 1006
DpnII GATC 5 cut(s) 51, 831, 851, 877, 1004
EaeI YGGCCR 1 cut(s) 424
Eam1104I CTCTTC 1 cut(s) 838
EarI CTCTTC 1 cut(s) 838
Ecl136II GAGCTC 1 cut(s) 133
Eco130I CCWWGG 1 cut(s) 756
Eco24I GRGCYC 1 cut(s) 135
Eco47I GGWCC 3 cut(s) 188, 413, 740
Eco53kI GAGCTC 1 cut(s) 133
Eco57I CTGAAG 1 cut(s) 941
Eco81I CCTNAGG 1 cut(s) 110
Eco88I CYCGRG 1 cut(s) 128
EcoICRI GAGCTC 1 cut(s) 133
EcoO109I RGGNCCY 2 cut(s) 188, 740
EcoRI GAATTC 2 cut(s) 216, 818
EcoT14I CCWWGG 1 cut(s) 756
EcoT38I GRGCYC 1 cut(s) 135
ErhI CCWWGG 1 cut(s) 756
FaeI CATG 1 cut(s) 369
FaiI YATR 9 cut(s) 141, 195, 292, 328, 367, 378, 380, 1021, 1023
FaqI GGGAC 3 cut(s) 426, 446, 721
FatI CATG 1 cut(s) 365
FauNDI CATATG 1 cut(s) 1021
FbaI TGATCA 1 cut(s) 51
FblI GTMKAC 1 cut(s) 46
Fnu4HI GCNGC 1 cut(s) 948
FokI GGATG 3 cut(s) 41, 61, 1012
FriOI GRGCYC 1 cut(s) 135
Fsp4HI GCNGC 1 cut(s) 948
FspBI CTAG 7 cut(s) 59, 105, 528, 609, 695, 776, 872
GluI GCNGC 1 cut(s) 948
GsuI CTGGAG 1 cut(s) 268
HaeIII GGCC 3 cut(s) 153, 337, 426
HapII CCGG 2 cut(s) 573, 724
HgaI GACGC 1 cut(s) 718
Hin1II CATG 1 cut(s) 369
HinfI GANTC 4 cut(s) 399, 459, 500, 865
HpaII CCGG 2 cut(s) 573, 724
HphI GGTGA 2 cut(s) 587, 634
Hpy166II GTNNAC 2 cut(s) 47, 371
Hpy188I TCNGA 2 cut(s) 208, 864
Hpy188III TCNNGA 4 cut(s) 112, 403, 504, 799
Hpy8I GTNNAC 2 cut(s) 47, 371
HpyAV CCTTC 6 cut(s) 179, 327, 475, 582, 613, 899
HpyCH4III ACNGT 2 cut(s) 375, 628
HpyCH4IV ACGT 2 cut(s) 144, 912
HpyCH4V TGCA 2 cut(s) 452, 598
HpyF10VI GCNNNNNNNGC 2 cut(s) 159, 449
HpyF3I CTNAG 2 cut(s) 110, 672
HpySE526I ACGT 2 cut(s) 144, 912
Hsp92II CATG 1 cut(s) 369
Ksp22I TGATCA 1 cut(s) 51
Kzo9I GATC 5 cut(s) 51, 831, 851, 877, 1004
LmnI GCTCC 3 cut(s) 39, 138, 887
Lsp1109I GCAGC 1 cut(s) 959
LweI GCATC 1 cut(s) 376
MaeI CTAG 7 cut(s) 59, 105, 528, 609, 695, 776, 872
MaeII ACGT 2 cut(s) 144, 912
MaeIII GTNAC 2 cut(s) 575, 622
MalI GATC 5 cut(s) 53, 833, 853, 879, 1006
MboI GATC 5 cut(s) 51, 831, 851, 877, 1004
MboII GAAGA 5 cut(s) 418, 855, 861, 914, 989
MflI RGATCY 1 cut(s) 877
MhlI GDGCHC 1 cut(s) 135
MluCI AATT 5 cut(s) 216, 287, 535, 818, 1015
MlyI GAGTC 3 cut(s) 393, 468, 859
MseI TTAA 4 cut(s) 354, 476, 534, 593
MspI CCGG 2 cut(s) 573, 724
MwoI GCNNNNNNNGC 2 cut(s) 159, 449
NdeI CATATG 1 cut(s) 1021
NdeII GATC 5 cut(s) 51, 831, 851, 877, 1004
NheI GCTAGC 1 cut(s) 104
NlaIII CATG 1 cut(s) 369
NlaIV GGNNCC 5 cut(s) 154, 234, 414, 742, 785
NmuCI GTSAC 2 cut(s) 575, 622
NspI RCATGY 1 cut(s) 369
PaeR7I CTCGAG 1 cut(s) 128
PciI ACATGT 1 cut(s) 365
PfeI GAWTC 1 cut(s) 500
PflMI CCANNNNNTGG 1 cut(s) 422
PkrI GCNGC 1 cut(s) 949
PleI GAGTC 3 cut(s) 393, 467, 859
PpsI GAGTC 3 cut(s) 393, 467, 859
PpuMI RGGWCCY 2 cut(s) 188, 740
PscI ACATGT 1 cut(s) 365
PshBI ATTAAT 1 cut(s) 534
PsiI TTATAA 1 cut(s) 141
Psp124BI GAGCTC 1 cut(s) 135
Psp1406I AACGTT 1 cut(s) 912
Psp5II RGGWCCY 2 cut(s) 188, 740
PspN4I GGNNCC 5 cut(s) 154, 234, 414, 742, 785
PspPI GGNCC 4 cut(s) 152, 188, 413, 740
PspPPI RGGWCCY 2 cut(s) 188, 740
PspXI VCTCGAGB 1 cut(s) 128
PstI CTGCAG 1 cut(s) 454
PsuI RGATCY 1 cut(s) 877
RsaI GTAC 2 cut(s) 613, 943
RsaNI GTAC 2 cut(s) 612, 942
SacI GAGCTC 1 cut(s) 135
SaqAI TTAA 4 cut(s) 354, 476, 534, 593
SatI GCNGC 1 cut(s) 948
Sau3AI GATC 5 cut(s) 51, 831, 851, 877, 1004
Sau96I GGNCC 4 cut(s) 152, 188, 413, 740
SchI GAGTC 3 cut(s) 393, 468, 859
SduI GDGCHC 1 cut(s) 135
SfaNI GCATC 1 cut(s) 376
SfcI CTRYAG 2 cut(s) 450, 882
Sfr274I CTCGAG 1 cut(s) 128
SinI GGWCC 3 cut(s) 188, 413, 740
SlaI CTCGAG 1 cut(s) 128
SmlI CTYRAG 2 cut(s) 128, 635
SmoI CTYRAG 2 cut(s) 128, 635
SpeI ACTAGT 1 cut(s) 775
Sse9I AATT 5 cut(s) 216, 287, 535, 818, 1015
SsiI CCGC 1 cut(s) 330
SspMI CTAG 7 cut(s) 59, 105, 528, 609, 695, 776, 872
SstI GAGCTC 1 cut(s) 135
StyI CCWWGG 1 cut(s) 756
TaaI ACNGT 2 cut(s) 375, 628
TaiI ACGT 2 cut(s) 147, 915
TaqI TCGA 5 cut(s) 71, 129, 171, 492, 736
TasI AATT 5 cut(s) 216, 287, 535, 818, 1015
TatI WGTACW 1 cut(s) 611
TfiI GAWTC 1 cut(s) 500
Tru1I TTAA 4 cut(s) 354, 476, 534, 593
Tru9I TTAA 4 cut(s) 354, 476, 534, 593
TscAI CASTG 1 cut(s) 345
TseFI GTSAC 2 cut(s) 575, 622
TseI GCWGC 1 cut(s) 947
Tsp45I GTSAC 2 cut(s) 575, 622
TspDTI ATGAA 5 cut(s) 12, 17, 333, 915, 1008
TspGWI ACGGA 3 cut(s) 536, 855, 1002
TspRI CASTG 1 cut(s) 345
Van91I CCANNNNNTGG 1 cut(s) 422
VpaK11BI GGWCC 3 cut(s) 188, 413, 740
VspI ATTAAT 1 cut(s) 534
XapI RAATTY 3 cut(s) 216, 818, 1015
XceI RCATGY 1 cut(s) 369
XhoI CTCGAG 1 cut(s) 128
XmiI GTMKAC 1 cut(s) 46
XspI CTAG 7 cut(s) 59, 105, 528, 609, 695, 776, 872
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.