Rh1BG027100

strictosidine

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1B
OG0000674
Physical Location & Seq
Forward (+)
3819354 .. 3826617
7264 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1BG027100.1

Sequence Viewer

Length: 438 bp
ATGGTGTACTTCACAGACACCAGTGCTATTTACCAGAGAAGAAATTGGGAATTGTCAGTGAGTACAGGTGATAAAACCGGAAGGTTAATGCGATATGATCCATGGACAAAGAATGTGACTGTGTTGCGTGATGACTTGTCTTTTGCAAATGGGGTGGCACTAAGCAAAGACGGTGATTTTGTGCTAGTAACCCAAACCACTGCAAAGAACATTTTGAGGTACTGGCTTCGAGGGCCTAGAGCCAACACTGTTGATATTTTTTTCCAACTGCGAGGAGCCCCAGACAATATCATCCGAAACATAAATGGAGAATTTTGGGTTGCGGAAAATAGTGCCGGAAAAGGTAAAGCGGTCAGGCTTGACGCTCAAGGAAATTTTCTGGACGTGGTAGATGGACTCAACCCGCATTTTATGGCCAATCTTATTAAAACTTATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Str_synth

Protein Analysis

145

Amino Acids

16.44

Weight (kDa)

9.3

Isoelectric Point (pI)

12.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Str_synth PF03088 2 - 78 1.3e-27 Strictosidine synthase
SGL PF08450 22 - 130 3.3e-12 SMP-30/Gluconolactonase/LRE-like region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 3 cut(s) 323, 350, 404
AclWI GGATC 1 cut(s) 92
AcoI YGGCCR 1 cut(s) 414
AcsI RAATTY 2 cut(s) 311, 373
AfaI GTAC 3 cut(s) 8, 64, 221
AjiI CACGTC 1 cut(s) 385
AlwI GGATC 1 cut(s) 92
AoxI GGCC 2 cut(s) 233, 414
ApoI RAATTY 2 cut(s) 311, 373
ArsI GACNNNNNNTTYG 4 cut(s) 125, 157, 161, 193
AspS9I GGNCC 1 cut(s) 233
AsuHPI GGTGA 2 cut(s) 80, 185
BalI TGGCCA 1 cut(s) 416
BanII GRGCYC 1 cut(s) 280
BccI CCATC 1 cut(s) 386
BfaI CTAG 2 cut(s) 185, 237
BmgBI CACGTC 1 cut(s) 385
BmgT120I GGNCC 1 cut(s) 233
BmiI GGNNCC 1 cut(s) 277
BpuEI CTTGAG 1 cut(s) 351
BsaJI CCNNGG 1 cut(s) 101
BsaWI WCCGGW 1 cut(s) 77
Bse1I ACTGG 2 cut(s) 21, 227
BseDI CCNNGG 1 cut(s) 101
BseGI GGATG 1 cut(s) 291
BseNI ACTGG 2 cut(s) 21, 227
BseRI GAGGAG 1 cut(s) 288
BshFI GGCC 2 cut(s) 235, 416
BsiSI CCGG 2 cut(s) 78, 336
BsnI GGCC 2 cut(s) 235, 416
Bsp1286I GDGCHC 1 cut(s) 280
Bsp143I GATC 1 cut(s) 97
Bsp19I CCATGG 1 cut(s) 101
BspACI CCGC 3 cut(s) 323, 350, 404
BspANI GGCC 2 cut(s) 235, 416
BspLI GGNNCC 1 cut(s) 277
BspPI GGATC 1 cut(s) 92
BsrI ACTGG 2 cut(s) 21, 227
BssECI CCNNGG 1 cut(s) 101
BssMI GATC 1 cut(s) 97
BssT1I CCWWGG 1 cut(s) 101
Bst4CI ACNGT 3 cut(s) 121, 173, 250
BstDEI CTNAG 1 cut(s) 161
BstDSI CCRYGG 1 cut(s) 101
BstF5I GGATG 1 cut(s) 291
BstKTI GATC 1 cut(s) 100
BstMBI GATC 1 cut(s) 97
BstMWI GCNNNNNNNGC 1 cut(s) 232
BsuRI GGCC 2 cut(s) 235, 416
BtgI CCRYGG 1 cut(s) 101
BtrI CACGTC 1 cut(s) 385
BtsCI GGATG 1 cut(s) 291
BtsI GCAGTG 1 cut(s) 198
BtsIMutI CAGTG 4 cut(s) 28, 63, 198, 246
Cfr13I GGNCC 1 cut(s) 233
CseI GACGC 1 cut(s) 371
Csp6I GTAC 3 cut(s) 7, 63, 220
CspCI CAANNNNNGTGG 2 cut(s) 135, 170
CviAII CATG 1 cut(s) 102
CviJI RGCY 6 cut(s) 226, 235, 242, 278, 358, 416
CviKI_1 RGCY 6 cut(s) 226, 235, 242, 278, 358, 416
CviQI GTAC 3 cut(s) 7, 63, 220
DdeI CTNAG 1 cut(s) 161
DpnI GATC 1 cut(s) 99
DpnII GATC 1 cut(s) 97
EaeI YGGCCR 1 cut(s) 414
Eco130I CCWWGG 1 cut(s) 101
Eco24I GRGCYC 1 cut(s) 280
EcoO109I RGGNCCY 1 cut(s) 233
EcoT14I CCWWGG 1 cut(s) 101
EcoT38I GRGCYC 1 cut(s) 280
ErhI CCWWGG 1 cut(s) 101
FaeI CATG 1 cut(s) 105
FaiI YATR 4 cut(s) 96, 103, 302, 413
FatI CATG 1 cut(s) 101
FauI CCCGC 1 cut(s) 411
FokI GGATG 1 cut(s) 278
FriOI GRGCYC 1 cut(s) 280
FspBI CTAG 2 cut(s) 185, 237
HaeIII GGCC 2 cut(s) 235, 416
HapII CCGG 2 cut(s) 78, 336
HgaI GACGC 1 cut(s) 371
Hin1II CATG 1 cut(s) 105
HinfI GANTC 1 cut(s) 396
HpaII CCGG 2 cut(s) 78, 336
HphI GGTGA 2 cut(s) 80, 185
Hpy166II GTNNAC 1 cut(s) 7
Hpy188I TCNGA 1 cut(s) 296
Hpy188III TCNNGA 1 cut(s) 380
Hpy8I GTNNAC 1 cut(s) 7
HpyAV CCTTC 1 cut(s) 75
HpyCH4III ACNGT 3 cut(s) 121, 173, 250
HpyCH4IV ACGT 1 cut(s) 384
HpyCH4V TGCA 2 cut(s) 146, 203
HpyF10VI GCNNNNNNNGC 1 cut(s) 232
HpyF3I CTNAG 1 cut(s) 161
HpySE526I ACGT 1 cut(s) 384
Hsp92II CATG 1 cut(s) 105
Kzo9I GATC 1 cut(s) 97
LmnI GCTCC 1 cut(s) 275
LpnPI CCDG 9 cut(s) 34, 47, 51, 91, 208, 294, 340, 349, 365
MaeI CTAG 2 cut(s) 185, 237
MaeII ACGT 1 cut(s) 384
MaeIII GTNAC 2 cut(s) 115, 187
MalI GATC 1 cut(s) 99
MboI GATC 1 cut(s) 97
MboII GAAGA 1 cut(s) 51
MhlI GDGCHC 1 cut(s) 280
MlsI TGGCCA 1 cut(s) 416
MluCI AATT 4 cut(s) 43, 50, 311, 373
MluNI TGGCCA 1 cut(s) 416
MlyI GAGTC 1 cut(s) 390
MmeI TCCRAC 1 cut(s) 289
MnlI CCTC 3 cut(s) 210, 224, 266
Mox20I TGGCCA 1 cut(s) 416
MscI TGGCCA 1 cut(s) 416
MseI TTAA 3 cut(s) 86, 426, 436
Msp20I TGGCCA 1 cut(s) 416
MspI CCGG 2 cut(s) 78, 336
MwoI GCNNNNNNNGC 1 cut(s) 232
NcoI CCATGG 1 cut(s) 101
NdeII GATC 1 cut(s) 97
NlaIII CATG 1 cut(s) 105
NlaIV GGNNCC 1 cut(s) 277
NmuCI GTSAC 1 cut(s) 115
PleI GAGTC 1 cut(s) 390
PpsI GAGTC 1 cut(s) 390
PspN4I GGNNCC 1 cut(s) 277
PspPI GGNCC 1 cut(s) 233
RsaI GTAC 3 cut(s) 8, 64, 221
RsaNI GTAC 3 cut(s) 7, 63, 220
SaqAI TTAA 3 cut(s) 86, 426, 436
Sau3AI GATC 1 cut(s) 97
Sau96I GGNCC 1 cut(s) 233
SchI GAGTC 1 cut(s) 390
SduI GDGCHC 1 cut(s) 280
SetI ASST 5 cut(s) 70, 86, 221, 346, 387
SmlI CTYRAG 1 cut(s) 366
SmoI CTYRAG 1 cut(s) 366
Sse9I AATT 4 cut(s) 43, 50, 311, 373
SsiI CCGC 3 cut(s) 323, 350, 404
SspMI CTAG 2 cut(s) 185, 237
StyI CCWWGG 1 cut(s) 101
TaaI ACNGT 3 cut(s) 121, 173, 250
TaiI ACGT 1 cut(s) 387
TaqI TCGA 1 cut(s) 229
TasI AATT 4 cut(s) 43, 50, 311, 373
TatI WGTACW 2 cut(s) 6, 62
Tru1I TTAA 3 cut(s) 86, 426, 436
Tru9I TTAA 3 cut(s) 86, 426, 436
TscAI CASTG 4 cut(s) 28, 63, 205, 253
TseFI GTSAC 1 cut(s) 115
Tsp45I GTSAC 1 cut(s) 115
TspRI CASTG 4 cut(s) 28, 63, 205, 253
XapI RAATTY 2 cut(s) 311, 373
XspI CTAG 2 cut(s) 185, 237
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.