Rroxscaffold_4G00329460

strictosidine

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0000674
Physical Location & Seq
Reverse (-)
62773309 .. 62774274
966 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00329460.1

Sequence Viewer

Length: 966 bp
ATGAAATCAGCTCTTGTTACACTCTTCTTCTTCTTCCTCTTTTTGTCTGTTCACCAAGCCCAAGGAGCATCTTTTTCACTCCCAAACCTAAACCATCATGAAATACCCATCCATGTACCGCAGCCCGAAAGCATTGCTTTCGACTGCAATGGTGCACCCTACGTGGGTGTGGCTGATGGCAGGATATTCAAGTGGCTAGGATACCCTTTTGGGTGGAGAGAATTCGCAGTCACCGCCCCCAATAGACCGAGAGGATTATGTGATTTAACAACCAACAAAGACAATGAACCGATATGCGGGAGGCCACTTGGTTTAAAATTTGACCTCACAACATGTGAACTGTATATCGCAGATGCCTATTTTGGGCTTCTGAAGACAGGACCAAATGGTGGAGCAGCTCAAATACTTGCGACATCTGCTCAAGGAGTTCCTTTCCGTTTCTTAAACGGTGTCGACATTGATCGCCAAAGTGGAATGGTGTACTTCACAGACACCAGTGCTATTTACCAGAGAAGAAATTGGGAATTGTCAGTTAGTACAGGTGATAAAACTGGAAGGTTAATGCGATATGATCCATGGACAAAGAATGTCACTGTGTTGCGTCAAGACTTGTCTTTTGCAAACGGGGTGGCACTAAGCAAAGACGGTGATTTTGTGCTAGTAACCCAAACCACTGCAAAGAACATTTTGAGGTATTGGCTTCGAGGGCCTAGAGCCAACACTGTTGATATTTTTTTCCAACTGCAAGGAGCCCCGGACAATATCATCAGAAACATAAATGGAGAATTTTGGGTTGCAGAAAATAGTGCCGGAAAAGGTAAAGCGGTCAAGCTTGACGCTCAAGGAAAATTTCTGGACGTGGTGGATGGACTCAACCCTGTGAGTCATGTTGAGCAGCGTTATAGAAACCTTTGGATAGGATCTGTGGTTAATAACTTTGTTGGGGTTATTCTTAATTTTGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Str_synth

Protein Analysis

321

Amino Acids

35.7

Weight (kDa)

8.61

Isoelectric Point (pI)

23.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Str_synth PF03088 149 - 236 5e-30 Strictosidine synthase
SGL PF08450 180 - 289 1e-10 SMP-30/Gluconolactonase/LRE-like region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 389
AccI GTMKAC 1 cut(s) 453
AciI CCGC 4 cut(s) 119, 234, 297, 824
AclWI GGATC 2 cut(s) 566, 928
AcsI RAATTY 4 cut(s) 221, 317, 785, 848
AcuI CTGAAG 1 cut(s) 392
AfaI GTAC 3 cut(s) 117, 482, 538
AfiI CCNNNNNNNGG 4 cut(s) 164, 296, 363, 389
AflIII ACRYGT 1 cut(s) 332
AgsI TTSAA 1 cut(s) 190
AjiI CACGTC 1 cut(s) 859
AluBI AGCT 3 cut(s) 11, 398, 832
AluI AGCT 3 cut(s) 11, 398, 832
Alw21I GWGCWC 1 cut(s) 157
Alw44I GTGCAC 1 cut(s) 153
AlwI GGATC 2 cut(s) 566, 928
AoxI GGCC 2 cut(s) 302, 707
ApaLI GTGCAC 1 cut(s) 153
ApeKI GCWGC 3 cut(s) 121, 395, 895
ApoI RAATTY 4 cut(s) 221, 317, 785, 848
ArsI GACNNNNNNTTYG 4 cut(s) 599, 631, 635, 667
AspS9I GGNCC 2 cut(s) 380, 707
AsuC2I CCSGG 1 cut(s) 755
AsuHPI GGTGA 4 cut(s) 44, 223, 554, 659
AvaII GGWCC 1 cut(s) 380
BaeGI GKGCMC 1 cut(s) 157
BanII GRGCYC 1 cut(s) 754
BbsI GAAGAC 1 cut(s) 380
Bbv12I GWGCWC 1 cut(s) 157
BbvI GCAGC 3 cut(s) 133, 407, 907
BccI CCATC 4 cut(s) 102, 116, 170, 860
BcgI CGANNNNNNTGC 4 cut(s) 116, 121, 150, 155
BciVI GTATCC 1 cut(s) 194
BcnI CCSGG 1 cut(s) 755
BfaI CTAG 3 cut(s) 197, 659, 711
BfuI GTATCC 1 cut(s) 194
BisI GCNGC 3 cut(s) 122, 396, 896
BlsI GCNGC 3 cut(s) 123, 397, 897
Bme1390I CCNGG 1 cut(s) 755
Bme18I GGWCC 1 cut(s) 380
BmgBI CACGTC 1 cut(s) 859
BmgT120I GGNCC 2 cut(s) 380, 707
BmiI GGNNCC 1 cut(s) 751
BmrFI CCNGG 1 cut(s) 755
BmsI GCATC 2 cut(s) 77, 343
BpiI GAAGAC 1 cut(s) 380
BpuEI CTTGAG 2 cut(s) 405, 825
BpuMI CCSGG 1 cut(s) 755
BsaAI YACGTR 1 cut(s) 163
BsaJI CCNNGG 3 cut(s) 61, 575, 753
Bsc4I CCNNNNNNNGG 4 cut(s) 164, 296, 363, 389
Bse1I ACTGG 2 cut(s) 495, 556
Bse3DI GCAATG 2 cut(s) 132, 154
BseDI CCNNGG 3 cut(s) 61, 575, 753
BseGI GGATG 2 cut(s) 108, 871
BseLI CCNNNNNNNGG 4 cut(s) 164, 296, 363, 389
BseMI GCAATG 2 cut(s) 132, 154
BseNI ACTGG 2 cut(s) 495, 556
BseSI GKGCMC 1 cut(s) 157
BseXI GCAGC 3 cut(s) 133, 407, 907
BshFI GGCC 2 cut(s) 304, 709
BsiHKAI GWGCWC 1 cut(s) 157
BsiSI CCGG 2 cut(s) 755, 810
BslI CCNNNNNNNGG 4 cut(s) 164, 296, 363, 389
BsnI GGCC 2 cut(s) 304, 709
Bsp1286I GDGCHC 2 cut(s) 157, 754
Bsp143I GATC 3 cut(s) 460, 571, 920
Bsp19I CCATGG 1 cut(s) 575
BspACI CCGC 4 cut(s) 119, 234, 297, 824
BspANI GGCC 2 cut(s) 304, 709
BspHI TCATGA 1 cut(s) 97
BspLI GGNNCC 1 cut(s) 751
BspPI GGATC 2 cut(s) 566, 928
BsrDI GCAATG 2 cut(s) 132, 154
BsrI ACTGG 2 cut(s) 495, 556
BssECI CCNNGG 3 cut(s) 61, 575, 753
BssMI GATC 3 cut(s) 460, 571, 920
BssT1I CCWWGG 2 cut(s) 61, 575
Bst4CI ACNGT 5 cut(s) 342, 449, 595, 647, 724
Bst6I CTCTTC 1 cut(s) 29
BstBAI YACGTR 1 cut(s) 163
BstDEI CTNAG 1 cut(s) 635
BstDSI CCRYGG 1 cut(s) 575
BstF5I GGATG 2 cut(s) 108, 871
BstKTI GATC 3 cut(s) 463, 574, 923
BstMBI GATC 3 cut(s) 460, 571, 920
BstMWI GCNNNNNNNGC 4 cut(s) 65, 233, 416, 706
BstNSI RCATGY 1 cut(s) 336
BstSCI CCNGG 1 cut(s) 753
BstSLI GKGCMC 1 cut(s) 157
BstV1I GCAGC 3 cut(s) 133, 407, 907
BstV2I GAAGAC 1 cut(s) 380
BstX2I RGATCY 1 cut(s) 920
BstYI RGATCY 1 cut(s) 920
BsuI GTATCC 1 cut(s) 194
BsuRI GGCC 2 cut(s) 304, 709
BtgI CCRYGG 1 cut(s) 575
BtrI CACGTC 1 cut(s) 859
BtsCI GGATG 2 cut(s) 108, 871
BtsI GCAGTG 1 cut(s) 672
BtsIMutI CAGTG 4 cut(s) 502, 591, 672, 720
CciI TCATGA 1 cut(s) 97
Cfr13I GGNCC 2 cut(s) 380, 707
CseI GACGC 2 cut(s) 590, 845
Csp6I GTAC 3 cut(s) 116, 481, 537
CspCI CAANNNNNGTGG 2 cut(s) 609, 644
CviAII CATG 5 cut(s) 98, 113, 333, 576, 887
CviQI GTAC 3 cut(s) 116, 481, 537
DdeI CTNAG 1 cut(s) 635
DpnI GATC 3 cut(s) 462, 573, 922
DpnII GATC 3 cut(s) 460, 571, 920
DraI TTTAAA 1 cut(s) 315
Eam1104I CTCTTC 1 cut(s) 29
EarI CTCTTC 1 cut(s) 29
Eco130I CCWWGG 2 cut(s) 61, 575
Eco24I GRGCYC 1 cut(s) 754
Eco47I GGWCC 1 cut(s) 380
Eco57I CTGAAG 1 cut(s) 392
EcoO109I RGGNCCY 1 cut(s) 707
EcoRI GAATTC 1 cut(s) 221
EcoT14I CCWWGG 2 cut(s) 61, 575
EcoT38I GRGCYC 1 cut(s) 754
ErhI CCWWGG 2 cut(s) 61, 575
FaeI CATG 5 cut(s) 101, 116, 336, 579, 890
FalI AAGNNNNNCTT 2 cut(s) 121, 153
FatI CATG 5 cut(s) 97, 112, 332, 575, 886
FauI CCCGC 1 cut(s) 290
FblI GTMKAC 1 cut(s) 453
Fnu4HI GCNGC 3 cut(s) 122, 396, 896
FokI GGATG 2 cut(s) 95, 878
FriOI GRGCYC 1 cut(s) 754
Fsp4HI GCNGC 3 cut(s) 122, 396, 896
FspBI CTAG 3 cut(s) 197, 659, 711
GluI GCNGC 3 cut(s) 122, 396, 896
HaeIII GGCC 2 cut(s) 304, 709
HapII CCGG 2 cut(s) 755, 810
HgaI GACGC 2 cut(s) 590, 845
Hin1II CATG 5 cut(s) 101, 116, 336, 579, 890
HincII GTYRAC 1 cut(s) 454
HindII GTYRAC 1 cut(s) 454
HindIII AAGCTT 1 cut(s) 830
HinfI GANTC 2 cut(s) 870, 883
HpaII CCGG 2 cut(s) 755, 810
HphI GGTGA 4 cut(s) 44, 223, 554, 659
Hpy166II GTNNAC 5 cut(s) 52, 155, 338, 454, 481
Hpy188I TCNGA 2 cut(s) 372, 770
Hpy188III TCNNGA 3 cut(s) 98, 605, 854
Hpy8I GTNNAC 5 cut(s) 52, 155, 338, 454, 481
HpyAV CCTTC 1 cut(s) 549
HpyCH4III ACNGT 5 cut(s) 342, 449, 595, 647, 724
HpyCH4IV ACGT 2 cut(s) 162, 858
HpyCH4V TGCA 6 cut(s) 147, 155, 620, 677, 745, 797
HpyF10VI GCNNNNNNNGC 4 cut(s) 65, 233, 416, 706
HpyF3I CTNAG 1 cut(s) 635
HpySE526I ACGT 2 cut(s) 162, 858
Hsp92II CATG 5 cut(s) 101, 116, 336, 579, 890
Kzo9I GATC 3 cut(s) 460, 571, 920
LmnI GCTCC 3 cut(s) 65, 392, 749
Lsp1109I GCAGC 3 cut(s) 133, 407, 907
LweI GCATC 2 cut(s) 77, 343
MaeI CTAG 3 cut(s) 197, 659, 711
MaeII ACGT 2 cut(s) 162, 858
MaeIII GTNAC 4 cut(s) 16, 229, 589, 661
MalI GATC 3 cut(s) 462, 573, 922
MboI GATC 3 cut(s) 460, 571, 920
MboII GAAGA 6 cut(s) 16, 19, 22, 25, 385, 525
MflI RGATCY 1 cut(s) 920
MhlI GDGCHC 2 cut(s) 157, 754
MluCI AATT 7 cut(s) 221, 317, 517, 524, 785, 848, 955
MlyI GAGTC 2 cut(s) 864, 892
MmeI TCCRAC 1 cut(s) 763
MnlI CCTC 6 cut(s) 47, 245, 294, 335, 684, 698
MseI TTAA 6 cut(s) 266, 314, 443, 560, 930, 954
MspI CCGG 2 cut(s) 755, 810
MspR9I CCNGG 1 cut(s) 755
MwoI GCNNNNNNNGC 4 cut(s) 65, 233, 416, 706
NciI CCSGG 1 cut(s) 755
NcoI CCATGG 1 cut(s) 575
NdeII GATC 3 cut(s) 460, 571, 920
NlaIII CATG 5 cut(s) 101, 116, 336, 579, 890
NlaIV GGNNCC 1 cut(s) 751
NmuCI GTSAC 2 cut(s) 229, 589
NspI RCATGY 1 cut(s) 336
PagI TCATGA 1 cut(s) 97
PciI ACATGT 1 cut(s) 332
PflMI CCANNNNNTGG 1 cut(s) 389
PkrI GCNGC 3 cut(s) 123, 397, 897
PleI GAGTC 2 cut(s) 864, 891
PpsI GAGTC 2 cut(s) 864, 891
Ppu21I YACGTR 1 cut(s) 163
PscI ACATGT 1 cut(s) 332
PspN4I GGNNCC 1 cut(s) 751
PspPI GGNCC 2 cut(s) 380, 707
PsuI RGATCY 1 cut(s) 920
RsaI GTAC 3 cut(s) 117, 482, 538
RsaNI GTAC 3 cut(s) 116, 481, 537
SalI GTCGAC 1 cut(s) 452
SaqAI TTAA 6 cut(s) 266, 314, 443, 560, 930, 954
SatI GCNGC 3 cut(s) 122, 396, 896
Sau3AI GATC 3 cut(s) 460, 571, 920
Sau96I GGNCC 2 cut(s) 380, 707
SchI GAGTC 2 cut(s) 864, 892
ScrFI CCNGG 1 cut(s) 755
SduI GDGCHC 2 cut(s) 157, 754
SfaNI GCATC 2 cut(s) 77, 343
SinI GGWCC 1 cut(s) 380
SmlI CTYRAG 2 cut(s) 420, 840
SmoI CTYRAG 2 cut(s) 420, 840
Sse9I AATT 7 cut(s) 221, 317, 517, 524, 785, 848, 955
SsiI CCGC 4 cut(s) 119, 234, 297, 824
SspMI CTAG 3 cut(s) 197, 659, 711
StyD4I CCNGG 1 cut(s) 753
StyI CCWWGG 2 cut(s) 61, 575
TaaI ACNGT 5 cut(s) 342, 449, 595, 647, 724
TaiI ACGT 2 cut(s) 165, 861
TaqI TCGA 3 cut(s) 141, 453, 703
TaqII GACCGA 1 cut(s) 262
TasI AATT 7 cut(s) 221, 317, 517, 524, 785, 848, 955
TatI WGTACW 2 cut(s) 480, 536
Tru1I TTAA 6 cut(s) 266, 314, 443, 560, 930, 954
Tru9I TTAA 6 cut(s) 266, 314, 443, 560, 930, 954
TscAI CASTG 4 cut(s) 502, 598, 679, 727
TseFI GTSAC 2 cut(s) 229, 589
TseI GCWGC 3 cut(s) 121, 395, 895
Tsp45I GTSAC 2 cut(s) 229, 589
TspDTI ATGAA 3 cut(s) 17, 114, 300
TspGWI ACGGA 1 cut(s) 425
TspRI CASTG 4 cut(s) 502, 598, 679, 727
Van91I CCANNNNNTGG 1 cut(s) 389
VneI GTGCAC 1 cut(s) 153
VpaK11BI GGWCC 1 cut(s) 380
XapI RAATTY 4 cut(s) 221, 317, 785, 848
XceI RCATGY 1 cut(s) 336
XmiI GTMKAC 1 cut(s) 453
XspI CTAG 3 cut(s) 197, 659, 711
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.