RLG00000024542

strictosidine

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0000674
Physical Location & Seq
Forward (+)
36881619 .. 36882602
984 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000024542

Sequence Viewer

Length: 984 bp
ATGAGTGTGAGATCAAGTTTCGTGGTTTTCTCGTTTTTCTTTTTGGTTACAATAGCCTCATCTATTTCTCAAGCAGAAGCTGTTTCAATTGGTGGTTTTGAACACCATGAAATTCCTGTTCCGGTAGTTGGCCCTGAGAGTGTAGCGTTTGACTGCAGCGGAGCAGCCTACGTCGGGGTTGCGGACGGGCGGATTTTTAAATGGCTTGGGAGTCAATATGGATGGGCGGAGTTTGCCACAACCTCCCCATCTAGGCCAAGAGGATTGTGCGACGGCTCCACTAACAAAGACAATGAGCCAACTTGTGGGAGGCCCCTCGGCCTCAAGTTCCACCCCACCACATGCGAGCTTTACATAGCCGATGCCTATTTCGGGCTCTTGAAGACAGGACCTAACGGCGGGGTAGCCCGACAACTTGCAACTTCAGCAGAAGGAATCCCTTTTCGGTTTACAAATTCTGTGGATATTAATCCGGTAACTGGAATGGTTTATTTCACAGATTCAAGCACGGTTTATCAAAGAAGGAATTGGGAGTCGTCATTCAACACCGGTGACAAAACTGGAAGACTAATGCGATATAATCCAAGTGGAAACCGCGTGGAAGTTTTGCTCCGTGGTTTGTCTTTTGCAAATGGTGTAGCTGTAAGTCTCGATGGTAATTTCGTTCTAGTAACCCAAACAACAAGCAAAAACATTCTAAGGTATTGGGTTCGAGGACCAAGAGCTACTAATTCAGGTGTAGGAGAACTTTTTGCACAAACACAAGGCTTCCCTGACAATATTAACAGAAACATAAACGGAGAGTTTTGGGTTGCAGAGAATAGTAATGGAAGAGGTAAAGCAGCCAAGTTCGATGCCTACGGGAACTTCATGGAAGCCTTGGATGGGCTTAATCCTATTAGCCATGCCCAAGAATTTCATCATAATCTGTGGATAGGTTCAATTGTCAACCCCTTTGTGGGCGTTGTTCGCAACATTTATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Str_synth

Protein Analysis

328

Amino Acids

35.69

Weight (kDa)

7.01

Isoelectric Point (pI)

26.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Str_synth PF03088 152 - 239 5.8e-29 Strictosidine synthase
SGL PF08450 183 - 292 1.7e-09 SMP-30/Gluconolactonase/LRE-like region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 305
AccII CGCG 1 cut(s) 597
AciI CCGC 6 cut(s) 159, 182, 190, 227, 399, 595
AcsI RAATTY 3 cut(s) 111, 454, 914
AcuI CTGAAG 1 cut(s) 408
AgeI ACCGGT 1 cut(s) 548
AgsI TTSAA 6 cut(s) 87, 101, 382, 504, 544, 942
AluBI AGCT 4 cut(s) 80, 349, 641, 725
AluI AGCT 4 cut(s) 80, 349, 641, 725
Alw26I GTCTC 1 cut(s) 653
AlwNI CAGNNNCTG 1 cut(s) 80
AoxI GGCC 4 cut(s) 130, 254, 311, 319
ApeKI GCWGC 3 cut(s) 156, 164, 842
ApoI RAATTY 3 cut(s) 111, 454, 914
AseI ATTAAT 1 cut(s) 468
AsiGI ACCGGT 1 cut(s) 548
AspS9I GGNCC 4 cut(s) 131, 312, 389, 716
AsuHPI GGTGA 1 cut(s) 563
AvaII GGWCC 2 cut(s) 389, 716
BanII GRGCYC 1 cut(s) 378
BbsI GAAGAC 2 cut(s) 389, 571
BbvI GCAGC 3 cut(s) 168, 176, 854
BccI CCATC 4 cut(s) 216, 256, 647, 878
BceAI ACGGC 2 cut(s) 289, 412
BcoDI GTCTC 1 cut(s) 653
BfaI CTAG 2 cut(s) 252, 668
BfmI CTRYAG 1 cut(s) 154
BisI GCNGC 3 cut(s) 157, 165, 843
BlsI GCNGC 3 cut(s) 158, 166, 844
Bme18I GGWCC 2 cut(s) 389, 716
BmgT120I GGNCC 4 cut(s) 131, 312, 389, 716
BmiI GGNNCC 2 cut(s) 277, 314
BmsI GCATC 2 cut(s) 352, 844
BpiI GAAGAC 2 cut(s) 389, 571
BpuEI CTTGAG 2 cut(s) 54, 308
BsaBI GATNNNNATC 1 cut(s) 468
BsaJI CCNNGG 3 cut(s) 316, 613, 879
BsaWI WCCGGW 3 cut(s) 121, 472, 548
Bse118I RCCGGY 1 cut(s) 548
Bse1I ACTGG 2 cut(s) 484, 565
Bse8I GATNNNNATC 1 cut(s) 468
BseDI CCNNGG 3 cut(s) 316, 613, 879
BseGI GGATG 2 cut(s) 227, 889
BseJI GATNNNNATC 1 cut(s) 468
BseMII CTCAG 1 cut(s) 126
BseNI ACTGG 2 cut(s) 484, 565
BseXI GCAGC 3 cut(s) 168, 176, 854
Bsh1236I CGCG 1 cut(s) 597
BshFI GGCC 4 cut(s) 132, 256, 313, 321
BshTI ACCGGT 1 cut(s) 548
BsiSI CCGG 3 cut(s) 122, 473, 549
BsmAI GTCTC 1 cut(s) 653
BsnI GGCC 4 cut(s) 132, 256, 313, 321
Bsp1286I GDGCHC 1 cut(s) 378
Bsp143I GATC 1 cut(s) 11
BspACI CCGC 6 cut(s) 159, 182, 190, 227, 399, 595
BspANI GGCC 4 cut(s) 132, 256, 313, 321
BspCNI CTCAG 1 cut(s) 127
BspFNI CGCG 1 cut(s) 597
BspLI GGNNCC 2 cut(s) 277, 314
BspMAI CTGCAG 1 cut(s) 158
BsrFI RCCGGY 1 cut(s) 548
BsrI ACTGG 2 cut(s) 484, 565
BssAI RCCGGY 1 cut(s) 548
BssECI CCNNGG 3 cut(s) 316, 613, 879
BssMI GATC 1 cut(s) 11
BssT1I CCWWGG 1 cut(s) 879
Bst4CI ACNGT 1 cut(s) 511
Bst6I CTCTTC 1 cut(s) 826
BstC8I GCNNGC 1 cut(s) 347
BstDEI CTNAG 2 cut(s) 135, 698
BstDSI CCRYGG 1 cut(s) 613
BstF5I GGATG 2 cut(s) 227, 889
BstFNI CGCG 1 cut(s) 597
BstKTI GATC 1 cut(s) 14
BstMAI GTCTC 1 cut(s) 653
BstMBI GATC 1 cut(s) 11
BstMWI GCNNNNNNNGC 3 cut(s) 233, 425, 969
BstNSI RCATGY 1 cut(s) 345
BstSFI CTRYAG 1 cut(s) 154
BstUI CGCG 1 cut(s) 597
BstV1I GCAGC 3 cut(s) 168, 176, 854
BstV2I GAAGAC 2 cut(s) 389, 571
BsuRI GGCC 4 cut(s) 132, 256, 313, 321
BtgI CCRYGG 1 cut(s) 613
BtsCI GGATG 2 cut(s) 227, 889
Cac8I GCNNGC 1 cut(s) 347
CaiI CAGNNNCTG 1 cut(s) 80
Cfr10I RCCGGY 1 cut(s) 548
Cfr13I GGNCC 4 cut(s) 131, 312, 389, 716
CspAI ACCGGT 1 cut(s) 548
CspCI CAANNNNNGTGG 3 cut(s) 38, 441, 476
CviAII CATG 4 cut(s) 107, 342, 871, 905
DdeI CTNAG 2 cut(s) 135, 698
DpnI GATC 1 cut(s) 13
DpnII GATC 1 cut(s) 11
DraI TTTAAA 1 cut(s) 199
Eam1104I CTCTTC 1 cut(s) 826
EarI CTCTTC 1 cut(s) 826
EciI GGCGGA 2 cut(s) 205, 242
Eco130I CCWWGG 1 cut(s) 879
Eco24I GRGCYC 1 cut(s) 378
Eco47I GGWCC 2 cut(s) 389, 716
Eco57I CTGAAG 1 cut(s) 408
EcoO109I RGGNCCY 2 cut(s) 312, 389
EcoT14I CCWWGG 1 cut(s) 879
EcoT38I GRGCYC 1 cut(s) 378
ErhI CCWWGG 1 cut(s) 879
FaeI CATG 4 cut(s) 110, 345, 874, 908
FaiI YATR 9 cut(s) 108, 219, 343, 356, 579, 794, 872, 906, 924
FatI CATG 4 cut(s) 106, 341, 870, 904
FauI CCCGC 1 cut(s) 392
Fnu4HI GCNGC 3 cut(s) 157, 165, 843
FokI GGATG 2 cut(s) 234, 896
FriOI GRGCYC 1 cut(s) 378
Fsp4HI GCNGC 3 cut(s) 157, 165, 843
FspBI CTAG 2 cut(s) 252, 668
GluI GCNGC 3 cut(s) 157, 165, 843
HaeIII GGCC 4 cut(s) 132, 256, 313, 321
HapII CCGG 3 cut(s) 122, 473, 549
Hin1II CATG 4 cut(s) 110, 345, 874, 908
HincII GTYRAC 1 cut(s) 949
HindII GTYRAC 1 cut(s) 949
HinfI GANTC 4 cut(s) 211, 435, 500, 533
HpaII CCGG 3 cut(s) 122, 473, 549
HphI GGTGA 1 cut(s) 563
Hpy166II GTNNAC 2 cut(s) 450, 949
Hpy188III TCNNGA 2 cut(s) 379, 650
Hpy8I GTNNAC 2 cut(s) 450, 949
Hpy99I CGWCG 2 cut(s) 176, 275
HpyAV CCTTC 2 cut(s) 425, 516
HpyCH4III ACNGT 1 cut(s) 511
HpyCH4IV ACGT 1 cut(s) 171
HpyCH4V TGCA 5 cut(s) 156, 419, 629, 755, 815
HpyF10VI GCNNNNNNNGC 3 cut(s) 233, 425, 969
HpyF3I CTNAG 2 cut(s) 135, 698
HpySE526I ACGT 1 cut(s) 171
Hsp92II CATG 4 cut(s) 110, 345, 874, 908
Kzo9I GATC 1 cut(s) 11
LmnI GCTCC 3 cut(s) 161, 281, 615
Lsp1109I GCAGC 3 cut(s) 168, 176, 854
LweI GCATC 2 cut(s) 352, 844
MaeI CTAG 2 cut(s) 252, 668
MaeII ACGT 1 cut(s) 171
MaeIII GTNAC 4 cut(s) 46, 475, 551, 670
MalI GATC 1 cut(s) 13
MboI GATC 1 cut(s) 11
MboII GAAGA 3 cut(s) 394, 576, 843
MfeI CAATTG 2 cut(s) 87, 942
MhlI GDGCHC 1 cut(s) 378
MluCI AATT 8 cut(s) 87, 111, 454, 526, 658, 730, 914, 942
MlyI GAGTC 2 cut(s) 220, 542
MnlI CCTC 8 cut(s) 67, 253, 254, 303, 326, 332, 707, 827
MseI TTAA 5 cut(s) 198, 468, 783, 891, 982
MspA1I CMGCKG 1 cut(s) 159
MspI CCGG 3 cut(s) 122, 473, 549
MunI CAATTG 2 cut(s) 87, 942
MvnI CGCG 1 cut(s) 597
MwoI GCNNNNNNNGC 3 cut(s) 233, 425, 969
NdeII GATC 1 cut(s) 11
NlaIII CATG 4 cut(s) 110, 345, 874, 908
NlaIV GGNNCC 2 cut(s) 277, 314
NmeAIII GCCGAG 1 cut(s) 297
NmuCI GTSAC 1 cut(s) 551
NspI RCATGY 1 cut(s) 345
PfeI GAWTC 2 cut(s) 435, 500
PflMI CCANNNNNTGG 1 cut(s) 305
PinAI ACCGGT 1 cut(s) 548
PkrI GCNGC 3 cut(s) 158, 166, 844
PleI GAGTC 2 cut(s) 219, 541
PpsI GAGTC 2 cut(s) 219, 541
PpuMI RGGWCCY 1 cut(s) 389
PshBI ATTAAT 1 cut(s) 468
Psp5II RGGWCCY 1 cut(s) 389
PspN4I GGNNCC 2 cut(s) 277, 314
PspPI GGNCC 4 cut(s) 131, 312, 389, 716
PspPPI RGGWCCY 1 cut(s) 389
PstI CTGCAG 1 cut(s) 158
PstNI CAGNNNCTG 1 cut(s) 80
SaqAI TTAA 5 cut(s) 198, 468, 783, 891, 982
SatI GCNGC 3 cut(s) 157, 165, 843
Sau3AI GATC 1 cut(s) 11
Sau96I GGNCC 4 cut(s) 131, 312, 389, 716
SchI GAGTC 2 cut(s) 220, 542
SduI GDGCHC 1 cut(s) 378
SfaNI GCATC 2 cut(s) 352, 844
SfcI CTRYAG 1 cut(s) 154
SgrAI CRCCGGYG 1 cut(s) 548
SinI GGWCC 2 cut(s) 389, 716
SmlI CTYRAG 2 cut(s) 69, 323
SmoI CTYRAG 2 cut(s) 69, 323
Sse9I AATT 8 cut(s) 87, 111, 454, 526, 658, 730, 914, 942
SsiI CCGC 6 cut(s) 159, 182, 190, 227, 399, 595
SspI AATATT 1 cut(s) 781
SspMI CTAG 2 cut(s) 252, 668
StyI CCWWGG 1 cut(s) 879
TaaI ACNGT 1 cut(s) 511
TaiI ACGT 1 cut(s) 174
TaqI TCGA 3 cut(s) 651, 712, 852
TasI AATT 8 cut(s) 87, 111, 454, 526, 658, 730, 914, 942
TfiI GAWTC 2 cut(s) 435, 500
Tru1I TTAA 5 cut(s) 198, 468, 783, 891, 982
Tru9I TTAA 5 cut(s) 198, 468, 783, 891, 982
TseFI GTSAC 1 cut(s) 551
TseI GCWGC 3 cut(s) 156, 164, 842
Tsp45I GTSAC 1 cut(s) 551
TspDTI ATGAA 3 cut(s) 123, 859, 908
TspGWI ACGGA 2 cut(s) 602, 813
Van91I CCANNNNNTGG 1 cut(s) 305
VpaK11BI GGWCC 2 cut(s) 389, 716
VspI ATTAAT 1 cut(s) 468
XapI RAATTY 3 cut(s) 111, 454, 914
XceI RCATGY 1 cut(s) 345
XspI CTAG 2 cut(s) 252, 668
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.