Rh1BG027300

strictosidine

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1B
OG0000674
Physical Location & Seq
Forward (+)
3835204 .. 3844370
9167 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1BG027300.1

Sequence Viewer

Length: 846 bp
ATGCCCGAAAGCATTGCTTTCGACTGCAATGGCGCACCCTGCGTGGGTGTGGCTGATGGCAGGATATTCAAGTGGCTACGATACCCTTTTGGGTGGAGAGAATTCGCAGTCACCGCCCCCAATAGGCCGAGAGGATTATGTGATTTAACAACCAACAAAGACAACGAACCGATATGCGGGAGGCCACTTGGTTTAAAATTTGACCTCACAACATGTGAACTGTATATCGCAGATGCCTATTTTGGGCTTCTAAAGACAGGACCAAATGGTGGAGTAGCTCAAATACTTGCGACATCTGCTGAAGGAGTTCCTTTCCGTTTCTTAAACGGTGTCGACATTGATCGCCCAAGTGGAATGGTGTACTTCACAGACACCAGTGCTACTTACCAGAGAAGAAATTGGGAATTGTCAGTTAGTACAGGTGATAAATCTGGAAGGTTAATGCGATATGATCCATGGACAAAGAATGTCACTGTGTTGCGTCATGACTTGTCTTTTGCAAACGGGGTGGCACTAAGCAAAGACGGTGATTTTGTGCTAGTAACCCAAACCACTGCAAAGAACATTTTGAGGTATTGGCTTCGAGGGCCTAGAGCCAACACTGTTGATATTTTTTTCCAACTGCAAGGAGCCCCAGACAATATCATCCGAAACATAAATGGAGAATTTTGGGTTGCGGAAAATAATGCTGGAAAAGGTAAAGCGGTCAAGCTTGACACCCAAGGAAATTTTCTGGACGTGGTAGATGGACTCAACCCTGTGAGTCATGTTGAGCAGCGTTATAGAAACCTTTGGATAGGATCTGTGGTTAATAACTTTGTTGGGGTTATTCTTAATTTTGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Str_synth

Protein Analysis

281

Amino Acids

31.25

Weight (kDa)

8.24

Isoelectric Point (pI)

21.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SGL PF08450 104 - 249 1.6e-11 SMP-30/Gluconolactonase/LRE-like region
Str_synth PF03088 109 - 196 1.7e-29 Strictosidine synthase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 269
AccI GTMKAC 1 cut(s) 333
AciI CCGC 4 cut(s) 114, 177, 677, 704
AclWI GGATC 2 cut(s) 446, 808
AcsI RAATTY 4 cut(s) 101, 197, 665, 727
AcuI CTGAAG 1 cut(s) 321
AfaI GTAC 2 cut(s) 362, 418
AfiI CCNNNNNNNGG 5 cut(s) 44, 123, 176, 243, 269
AflIII ACRYGT 1 cut(s) 212
AgsI TTSAA 1 cut(s) 70
AjiI CACGTC 1 cut(s) 739
AjuI GAANNNNNNNTTGG 2 cut(s) 714, 746
AluBI AGCT 2 cut(s) 278, 712
AluI AGCT 2 cut(s) 278, 712
AlwI GGATC 2 cut(s) 446, 808
AoxI GGCC 3 cut(s) 125, 182, 587
ApeKI GCWGC 1 cut(s) 775
ApoI RAATTY 4 cut(s) 101, 197, 665, 727
ArsI GACNNNNNNTTYG 4 cut(s) 479, 511, 515, 547
Asp700I GAANNNNTTC 1 cut(s) 306
AspLEI GCGC 1 cut(s) 35
AspS9I GGNCC 2 cut(s) 260, 587
AsuHPI GGTGA 3 cut(s) 103, 434, 539
AvaII GGWCC 1 cut(s) 260
BanII GRGCYC 1 cut(s) 634
BbvI GCAGC 1 cut(s) 787
BccI CCATC 2 cut(s) 50, 740
BcgI CGANNNNNNTGC 2 cut(s) 30, 35
BfaI CTAG 2 cut(s) 539, 591
BisI GCNGC 1 cut(s) 776
BlsI GCNGC 1 cut(s) 777
Bme18I GGWCC 1 cut(s) 260
BmgBI CACGTC 1 cut(s) 739
BmgT120I GGNCC 2 cut(s) 260, 587
BmiI GGNNCC 1 cut(s) 631
BmsI GCATC 1 cut(s) 223
BsaJI CCNNGG 2 cut(s) 455, 721
Bsc4I CCNNNNNNNGG 5 cut(s) 44, 123, 176, 243, 269
Bse1I ACTGG 1 cut(s) 375
Bse3DI GCAATG 2 cut(s) 12, 34
BseDI CCNNGG 2 cut(s) 455, 721
BseGI GGATG 1 cut(s) 645
BseLI CCNNNNNNNGG 5 cut(s) 44, 123, 176, 243, 269
BseMI GCAATG 2 cut(s) 12, 34
BseNI ACTGG 1 cut(s) 375
BseXI GCAGC 1 cut(s) 787
BshFI GGCC 3 cut(s) 127, 184, 589
BslI CCNNNNNNNGG 5 cut(s) 44, 123, 176, 243, 269
BsnI GGCC 3 cut(s) 127, 184, 589
Bsp1286I GDGCHC 1 cut(s) 634
Bsp143I GATC 3 cut(s) 340, 451, 800
Bsp19I CCATGG 1 cut(s) 455
BspACI CCGC 4 cut(s) 114, 177, 677, 704
BspANI GGCC 3 cut(s) 127, 184, 589
BspHI TCATGA 1 cut(s) 484
BspLI GGNNCC 1 cut(s) 631
BspPI GGATC 2 cut(s) 446, 808
BsrDI GCAATG 2 cut(s) 12, 34
BsrI ACTGG 1 cut(s) 375
BssECI CCNNGG 2 cut(s) 455, 721
BssMI GATC 3 cut(s) 340, 451, 800
BssT1I CCWWGG 2 cut(s) 455, 721
Bst4CI ACNGT 5 cut(s) 222, 329, 475, 527, 604
BstDEI CTNAG 1 cut(s) 515
BstDSI CCRYGG 1 cut(s) 455
BstF5I GGATG 1 cut(s) 645
BstHHI GCGC 1 cut(s) 35
BstKTI GATC 3 cut(s) 343, 454, 803
BstMBI GATC 3 cut(s) 340, 451, 800
BstMWI GCNNNNNNNGC 4 cut(s) 39, 113, 296, 586
BstNSI RCATGY 1 cut(s) 216
BstV1I GCAGC 1 cut(s) 787
BstX2I RGATCY 1 cut(s) 800
BstYI RGATCY 1 cut(s) 800
BsuRI GGCC 3 cut(s) 127, 184, 589
BtgI CCRYGG 1 cut(s) 455
BtrI CACGTC 1 cut(s) 739
BtsCI GGATG 1 cut(s) 645
BtsI GCAGTG 1 cut(s) 552
BtsIMutI CAGTG 4 cut(s) 382, 471, 552, 600
CciI TCATGA 1 cut(s) 484
CfoI GCGC 1 cut(s) 35
Cfr13I GGNCC 2 cut(s) 260, 587
CseI GACGC 1 cut(s) 470
Csp6I GTAC 2 cut(s) 361, 417
CspCI CAANNNNNGTGG 2 cut(s) 489, 524
CviAII CATG 4 cut(s) 213, 456, 485, 767
CviQI GTAC 2 cut(s) 361, 417
DdeI CTNAG 1 cut(s) 515
DpnI GATC 3 cut(s) 342, 453, 802
DpnII GATC 3 cut(s) 340, 451, 800
DraI TTTAAA 1 cut(s) 195
Eco130I CCWWGG 2 cut(s) 455, 721
Eco24I GRGCYC 1 cut(s) 634
Eco47I GGWCC 1 cut(s) 260
Eco57I CTGAAG 1 cut(s) 321
EcoO109I RGGNCCY 1 cut(s) 587
EcoRI GAATTC 1 cut(s) 101
EcoT14I CCWWGG 2 cut(s) 455, 721
EcoT38I GRGCYC 1 cut(s) 634
ErhI CCWWGG 2 cut(s) 455, 721
FaeI CATG 4 cut(s) 216, 459, 488, 770
FalI AAGNNNNNCTT 1 cut(s) 33
FatI CATG 4 cut(s) 212, 455, 484, 766
FauI CCCGC 1 cut(s) 170
FblI GTMKAC 1 cut(s) 333
Fnu4HI GCNGC 1 cut(s) 776
FokI GGATG 1 cut(s) 632
FriOI GRGCYC 1 cut(s) 634
Fsp4HI GCNGC 1 cut(s) 776
FspBI CTAG 2 cut(s) 539, 591
GlaI GCGC 1 cut(s) 34
GluI GCNGC 1 cut(s) 776
HaeIII GGCC 3 cut(s) 127, 184, 589
HgaI GACGC 1 cut(s) 470
HhaI GCGC 1 cut(s) 35
Hin1II CATG 4 cut(s) 216, 459, 488, 770
Hin6I GCGC 1 cut(s) 33
HinP1I GCGC 1 cut(s) 33
HincII GTYRAC 1 cut(s) 334
HindII GTYRAC 1 cut(s) 334
HindIII AAGCTT 1 cut(s) 710
HinfI GANTC 2 cut(s) 750, 763
HphI GGTGA 3 cut(s) 103, 434, 539
Hpy166II GTNNAC 3 cut(s) 218, 334, 361
Hpy188I TCNGA 1 cut(s) 650
Hpy188III TCNNGA 3 cut(s) 432, 485, 734
Hpy8I GTNNAC 3 cut(s) 218, 334, 361
HpyAV CCTTC 2 cut(s) 296, 429
HpyCH4III ACNGT 5 cut(s) 222, 329, 475, 527, 604
HpyCH4IV ACGT 1 cut(s) 738
HpyCH4V TGCA 4 cut(s) 27, 500, 557, 625
HpyF10VI GCNNNNNNNGC 4 cut(s) 39, 113, 296, 586
HpyF3I CTNAG 1 cut(s) 515
HpySE526I ACGT 1 cut(s) 738
Hsp92II CATG 4 cut(s) 216, 459, 488, 770
HspAI GCGC 1 cut(s) 33
Kzo9I GATC 3 cut(s) 340, 451, 800
LmnI GCTCC 1 cut(s) 629
Lsp1109I GCAGC 1 cut(s) 787
LweI GCATC 1 cut(s) 223
MaeI CTAG 2 cut(s) 539, 591
MaeII ACGT 1 cut(s) 738
MaeIII GTNAC 3 cut(s) 109, 469, 541
MalI GATC 3 cut(s) 342, 453, 802
MboI GATC 3 cut(s) 340, 451, 800
MboII GAAGA 1 cut(s) 405
MflI RGATCY 1 cut(s) 800
MhlI GDGCHC 1 cut(s) 634
MluCI AATT 7 cut(s) 101, 197, 397, 404, 665, 727, 835
MlyI GAGTC 2 cut(s) 744, 772
MmeI TCCRAC 1 cut(s) 643
MnlI CCTC 5 cut(s) 125, 174, 215, 564, 578
MroXI GAANNNNTTC 1 cut(s) 306
MseI TTAA 6 cut(s) 146, 194, 323, 440, 810, 834
MwoI GCNNNNNNNGC 4 cut(s) 39, 113, 296, 586
NcoI CCATGG 1 cut(s) 455
NdeII GATC 3 cut(s) 340, 451, 800
NlaIII CATG 4 cut(s) 216, 459, 488, 770
NlaIV GGNNCC 1 cut(s) 631
NmeAIII GCCGAG 1 cut(s) 153
NmuCI GTSAC 2 cut(s) 109, 469
NspI RCATGY 1 cut(s) 216
PagI TCATGA 1 cut(s) 484
PciI ACATGT 1 cut(s) 212
PdmI GAANNNNTTC 1 cut(s) 306
PflMI CCANNNNNTGG 1 cut(s) 269
PkrI GCNGC 1 cut(s) 777
PleI GAGTC 2 cut(s) 744, 771
PpsI GAGTC 2 cut(s) 744, 771
PscI ACATGT 1 cut(s) 212
PspN4I GGNNCC 1 cut(s) 631
PspPI GGNCC 2 cut(s) 260, 587
PsuI RGATCY 1 cut(s) 800
RsaI GTAC 2 cut(s) 362, 418
RsaNI GTAC 2 cut(s) 361, 417
SalI GTCGAC 1 cut(s) 332
SaqAI TTAA 6 cut(s) 146, 194, 323, 440, 810, 834
SatI GCNGC 1 cut(s) 776
Sau3AI GATC 3 cut(s) 340, 451, 800
Sau96I GGNCC 2 cut(s) 260, 587
SchI GAGTC 2 cut(s) 744, 772
SduI GDGCHC 1 cut(s) 634
SetI ASST 9 cut(s) 207, 280, 424, 440, 575, 700, 714, 741, 792
SfaNI GCATC 1 cut(s) 223
SinI GGWCC 1 cut(s) 260
Sse9I AATT 7 cut(s) 101, 197, 397, 404, 665, 727, 835
SsiI CCGC 4 cut(s) 114, 177, 677, 704
SspMI CTAG 2 cut(s) 539, 591
StyI CCWWGG 2 cut(s) 455, 721
TaaI ACNGT 5 cut(s) 222, 329, 475, 527, 604
TaiI ACGT 1 cut(s) 741
TaqI TCGA 3 cut(s) 21, 333, 583
TasI AATT 7 cut(s) 101, 197, 397, 404, 665, 727, 835
TatI WGTACW 2 cut(s) 360, 416
Tru1I TTAA 6 cut(s) 146, 194, 323, 440, 810, 834
Tru9I TTAA 6 cut(s) 146, 194, 323, 440, 810, 834
TscAI CASTG 4 cut(s) 382, 478, 559, 607
TseFI GTSAC 2 cut(s) 109, 469
TseI GCWGC 1 cut(s) 775
Tsp45I GTSAC 2 cut(s) 109, 469
TspGWI ACGGA 1 cut(s) 305
TspRI CASTG 4 cut(s) 382, 478, 559, 607
Van91I CCANNNNNTGG 1 cut(s) 269
VpaK11BI GGWCC 1 cut(s) 260
XapI RAATTY 4 cut(s) 101, 197, 665, 727
XceI RCATGY 1 cut(s) 216
XmiI GTMKAC 1 cut(s) 333
XmnI GAANNNNTTC 1 cut(s) 306
XspI CTAG 2 cut(s) 539, 591
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.