Rroxscaffold_6G00414030

strictosidine

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0000674
Physical Location & Seq
Forward (+)
36364081 .. 36368493
4413 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00414030.1

Sequence Viewer

Length: 1254 bp
ATGGCCGGCAAAGGAGAGGGACCGGCCATCGGTATCGATCTCGGTACCACTTACTCTTGCGTCGGCGTCTGGCAACACGACCGTGTTGAGATCATCGCCAACGACCAGGGAAACCGGACGACCCCGTCGTATGTCGCCTTCACCGACACCGAGAGGTTGATCGGAGACGCCGCTAAGAACCAGGTCGCCATGAACCCAATCAACACTGTTTTTGGTAAGATCTCAGATCCAGTTGGTCTTTTGATTTCAAATCAAGCGAAGTATTCAGCCATGAGTGTGAGATCAAGTTTCGTGGTTTTCTCGTTTTTCTTTTTGGTTACAATAGCCTCATCTATTTCTCAAGCAGAAGCTCTTTCAATTGGTGGTTTTGAACACCATGAAATTCCTGTTCCGGTAGTTGGCCCTGAGAGTGTGGCGTTTGACTGCAGCGGAGCAGCCTACGTGGGGGTTGCGGACGGGCGGATTTTTAAATGGCTTGGGAGGCAATATGGATGGGCGGAGTTTGCCACAACCTCCCCATCTAGGCCAAGAGCATTGTGCGACGGCTCCACCAACAAAGACAATGAGCCGACTTGTGGGAGACCCCTCGGCCTCAAGTTCCACCCCACCACCTGCGAGCTTTACATAGCCGATGCCTATTTCGGGCTCTTGAAGACAGGACCTAACGGCGGGGTAGCCCGACAACTTGCAACTTCAGCAGAAGGAATCCCTTTTGGGTTTACAAATTCTGTGGATATTAATCCCGTAACTGGAATGGTTTATTTCACTGATTCAAGCTCGGTTTATCAAAGAAGGAATTGGGAGTCGTCATTCAACACCGGTGACAAAACTGGAAGACTAATGCGATATAATCCTAGTGCAAACCGCGTGGAAGTTTTGCTCCGTGGTTTGTCTTTTGCAAATGGTGTAGCCGTAAGCCTAGATGGTAATTTCGTGCTAGTAACCCAAACAACAAGCAAAAACATTCTAAGGTATTGGGTTCGAGGACCAAGAGTTACTAATTCAGGTGTAGGAGAACTTTTTGCACAAACACAAGGCTTCCCTGACAATATTAACAGAAACATAAACGGAGAGTTTTGGGTTGCAGAGAATAGTAATGGAAGAGGTAAAGCAGCCAAGTTCGATGCCTACGGGAACTTCATGGAAGCCTTGGATGGGCTTAATCCTATTAGCCATGCCCAAGAATTTCATCATAATCTTTGGATAGGTTCGATTGTCAACCCCTTTGTGGGCGTTGTTCGCAACATTTATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Str_synth

Protein Analysis

417

Amino Acids

45.23

Weight (kDa)

6.4

Isoelectric Point (pI)

23.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HSP70 PF00012 9 - 72 1.1e-26 Hsp70 protein
Str_synth PF03088 242 - 329 3.5e-29 Strictosidine synthase
SGL PF08450 273 - 382 1.5e-09 SMP-30/Gluconolactonase/LRE-like region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 620
AasI GACNNNNNNGTC 1 cut(s) 124
Acc36I ACCTGC 1 cut(s) 620
Acc65I GGTACC 1 cut(s) 44
AccB1I GGYRCC 1 cut(s) 44
AccII CGCG 1 cut(s) 867
AciI CCGC 7 cut(s) 171, 429, 452, 460, 497, 669, 865
AclWI GGATC 1 cut(s) 221
AcoI YGGCCR 2 cut(s) 3, 24
AcsI RAATTY 3 cut(s) 381, 724, 1184
AcuI CTGAAG 1 cut(s) 678
AcyI GRCGYC 2 cut(s) 66, 168
AfaI GTAC 1 cut(s) 46
AgeI ACCGGT 1 cut(s) 818
AgsI TTSAA 6 cut(s) 249, 357, 371, 652, 774, 814
AjnI CCWGG 2 cut(s) 105, 180
AleI CACNNNNGTG 1 cut(s) 81
AluBI AGCT 3 cut(s) 350, 619, 777
AluI AGCT 3 cut(s) 350, 619, 777
Alw26I GTCTC 2 cut(s) 159, 574
AlwI GGATC 1 cut(s) 221
AoxI GGCC 5 cut(s) 3, 24, 400, 524, 589
ApeKI GCWGC 3 cut(s) 426, 434, 1112
ApoI RAATTY 3 cut(s) 381, 724, 1184
AseI ATTAAT 1 cut(s) 738
AsiGI ACCGGT 1 cut(s) 818
Asp718I GGTACC 1 cut(s) 44
AspS9I GGNCC 4 cut(s) 20, 401, 659, 986
AsuHPI GGTGA 2 cut(s) 133, 833
AvaII GGWCC 3 cut(s) 20, 659, 986
BanI GGYRCC 1 cut(s) 44
BanII GRGCYC 1 cut(s) 648
BarI GAAGNNNNNNTAC 2 cut(s) 122, 154
BbsI GAAGAC 2 cut(s) 659, 841
BbvI GCAGC 3 cut(s) 438, 446, 1124
BccI CCATC 5 cut(s) 35, 486, 526, 917, 1148
BceAI ACGGC 3 cut(s) 559, 682, 896
BciT130I CCWGG 2 cut(s) 107, 182
BcoDI GTCTC 2 cut(s) 159, 574
BfaI CTAG 4 cut(s) 522, 855, 920, 938
BfmI CTRYAG 1 cut(s) 424
BfuAI ACCTGC 1 cut(s) 620
BglII AGATCT 1 cut(s) 219
BisI GCNGC 4 cut(s) 171, 427, 435, 1113
BlsI GCNGC 4 cut(s) 172, 428, 436, 1114
Bme1390I CCNGG 2 cut(s) 107, 182
Bme18I GGWCC 3 cut(s) 20, 659, 986
BmgT120I GGNCC 4 cut(s) 20, 401, 659, 986
BmiI GGNNCC 3 cut(s) 21, 46, 547
BmrFI CCNGG 2 cut(s) 107, 182
BmsI GCATC 2 cut(s) 622, 1114
BpiI GAAGAC 2 cut(s) 659, 841
BpuEI CTTGAG 2 cut(s) 324, 578
Bsa29I ATCGAT 1 cut(s) 36
BsaAI YACGTR 1 cut(s) 442
BsaBI GATNNNNATC 1 cut(s) 738
BsaHI GRCGYC 2 cut(s) 66, 168
BsaI GGTCTC 1 cut(s) 574
BsaJI CCNNGG 4 cut(s) 106, 586, 883, 1149
BsaWI WCCGGW 3 cut(s) 114, 391, 818
Bse118I RCCGGY 3 cut(s) 5, 22, 818
Bse1I ACTGG 3 cut(s) 230, 754, 835
Bse8I GATNNNNATC 1 cut(s) 738
BseBI CCWGG 2 cut(s) 107, 182
BseCI ATCGAT 1 cut(s) 36
BseDI CCNNGG 4 cut(s) 106, 586, 883, 1149
BseGI GGATG 2 cut(s) 497, 1159
BseJI GATNNNNATC 1 cut(s) 738
BseMII CTCAG 2 cut(s) 237, 396
BseNI ACTGG 3 cut(s) 230, 754, 835
BseXI GCAGC 3 cut(s) 438, 446, 1124
Bsh1236I CGCG 1 cut(s) 867
Bsh1285I CGRYCG 1 cut(s) 82
BshFI GGCC 5 cut(s) 5, 26, 402, 526, 591
BshNI GGYRCC 1 cut(s) 44
BshTI ACCGGT 1 cut(s) 818
BshVI ATCGAT 1 cut(s) 36
BsiEI CGRYCG 1 cut(s) 82
BsiSI CCGG 5 cut(s) 6, 23, 115, 392, 819
BslFI GGGAC 1 cut(s) 33
BsmAI GTCTC 2 cut(s) 159, 574
BsmBI CGTCTC 1 cut(s) 159
BsmFI GGGAC 1 cut(s) 33
BsnI GGCC 5 cut(s) 5, 26, 402, 526, 591
Bso31I GGTCTC 1 cut(s) 574
Bsp1286I GDGCHC 1 cut(s) 648
Bsp143I GATC 6 cut(s) 37, 90, 159, 219, 226, 281
BspACI CCGC 7 cut(s) 171, 429, 452, 460, 497, 669, 865
BspANI GGCC 5 cut(s) 5, 26, 402, 526, 591
BspCNI CTCAG 2 cut(s) 236, 397
BspDI ATCGAT 1 cut(s) 36
BspFNI CGCG 1 cut(s) 867
BspLI GGNNCC 3 cut(s) 21, 46, 547
BspMAI CTGCAG 1 cut(s) 428
BspMI ACCTGC 1 cut(s) 620
BspPI GGATC 1 cut(s) 221
BspT107I GGYRCC 1 cut(s) 44
BspTNI GGTCTC 1 cut(s) 574
BsrFI RCCGGY 3 cut(s) 5, 22, 818
BsrI ACTGG 3 cut(s) 230, 754, 835
BssAI RCCGGY 3 cut(s) 5, 22, 818
BssECI CCNNGG 4 cut(s) 106, 586, 883, 1149
BssMI GATC 6 cut(s) 37, 90, 159, 219, 226, 281
BssNI GRCGYC 2 cut(s) 66, 168
BssT1I CCWWGG 1 cut(s) 1149
Bst2UI CCWGG 2 cut(s) 107, 182
Bst4CI ACNGT 2 cut(s) 83, 208
Bst6I CTCTTC 1 cut(s) 1096
BstACI GRCGYC 2 cut(s) 66, 168
BstBAI YACGTR 1 cut(s) 442
BstC8I GCNNGC 2 cut(s) 7, 617
BstDEI CTNAG 4 cut(s) 174, 223, 405, 968
BstDSI CCRYGG 1 cut(s) 883
BstF5I GGATG 2 cut(s) 497, 1159
BstFNI CGCG 1 cut(s) 867
BstKTI GATC 6 cut(s) 40, 93, 162, 222, 229, 284
BstMAI GTCTC 2 cut(s) 159, 574
BstMBI GATC 6 cut(s) 37, 90, 159, 219, 226, 281
BstMCI CGRYCG 1 cut(s) 82
BstMWI GCNNNNNNNGC 4 cut(s) 481, 503, 695, 1239
BstNI CCWGG 2 cut(s) 107, 182
BstSCI CCNGG 2 cut(s) 105, 180
BstSFI CTRYAG 1 cut(s) 424
BstUI CGCG 1 cut(s) 867
BstV1I GCAGC 3 cut(s) 438, 446, 1124
BstV2I GAAGAC 2 cut(s) 659, 841
BstX2I RGATCY 2 cut(s) 219, 226
BstYI RGATCY 2 cut(s) 219, 226
Bsu15I ATCGAT 1 cut(s) 36
BsuRI GGCC 5 cut(s) 5, 26, 402, 526, 591
BsuTUI ATCGAT 1 cut(s) 36
BtgI CCRYGG 1 cut(s) 883
BtgZI GCGATG 1 cut(s) 79
BtsCI GGATG 2 cut(s) 497, 1159
BtsIMutI CAGTG 2 cut(s) 204, 765
BveI ACCTGC 1 cut(s) 620
Cac8I GCNNGC 2 cut(s) 7, 617
Cfr10I RCCGGY 3 cut(s) 5, 22, 818
Cfr13I GGNCC 4 cut(s) 20, 401, 659, 986
ClaI ATCGAT 1 cut(s) 36
CseI GACGC 3 cut(s) 49, 55, 176
CsiI ACCWGGT 1 cut(s) 180
Csp6I GTAC 1 cut(s) 45
CspAI ACCGGT 1 cut(s) 818
CspCI CAANNNNNGTGG 6 cut(s) 273, 308, 711, 746, 849, 884
CviAII CATG 5 cut(s) 190, 271, 377, 1141, 1175
CviQI GTAC 1 cut(s) 45
DdeI CTNAG 4 cut(s) 174, 223, 405, 968
DpnI GATC 6 cut(s) 39, 92, 161, 221, 228, 283
DpnII GATC 6 cut(s) 37, 90, 159, 219, 226, 281
DraI TTTAAA 1 cut(s) 469
DrdI GACNNNNNNGTC 1 cut(s) 124
DseDI GACNNNNNNGTC 1 cut(s) 124
EaeI YGGCCR 2 cut(s) 3, 24
Eam1104I CTCTTC 1 cut(s) 1096
EarI CTCTTC 1 cut(s) 1096
EciI GGCGGA 2 cut(s) 475, 512
Eco130I CCWWGG 1 cut(s) 1149
Eco24I GRGCYC 1 cut(s) 648
Eco31I GGTCTC 1 cut(s) 574
Eco47I GGWCC 3 cut(s) 20, 659, 986
Eco57I CTGAAG 1 cut(s) 678
EcoO109I RGGNCCY 1 cut(s) 659
EcoRII CCWGG 2 cut(s) 105, 180
EcoT14I CCWWGG 1 cut(s) 1149
EcoT38I GRGCYC 1 cut(s) 648
ErhI CCWWGG 1 cut(s) 1149
Esp3I CGTCTC 1 cut(s) 159
FaeI CATG 5 cut(s) 193, 274, 380, 1144, 1178
FaqI GGGAC 1 cut(s) 33
FatI CATG 5 cut(s) 189, 270, 376, 1140, 1174
FauI CCCGC 1 cut(s) 662
Fnu4HI GCNGC 4 cut(s) 171, 427, 435, 1113
FokI GGATG 2 cut(s) 504, 1166
FriOI GRGCYC 1 cut(s) 648
Fsp4HI GCNGC 4 cut(s) 171, 427, 435, 1113
FspBI CTAG 4 cut(s) 522, 855, 920, 938
GluI GCNGC 4 cut(s) 171, 427, 435, 1113
HaeIII GGCC 5 cut(s) 5, 26, 402, 526, 591
HapII CCGG 5 cut(s) 6, 23, 115, 392, 819
HgaI GACGC 3 cut(s) 49, 55, 176
Hin1I GRCGYC 2 cut(s) 66, 168
Hin1II CATG 5 cut(s) 193, 274, 380, 1144, 1178
HincII GTYRAC 1 cut(s) 1219
HindII GTYRAC 1 cut(s) 1219
HinfI GANTC 3 cut(s) 705, 770, 803
HpaII CCGG 5 cut(s) 6, 23, 115, 392, 819
HphI GGTGA 2 cut(s) 133, 833
Hpy166II GTNNAC 2 cut(s) 720, 1219
Hpy188I TCNGA 2 cut(s) 164, 226
Hpy188III TCNNGA 1 cut(s) 649
Hpy8I GTNNAC 2 cut(s) 720, 1219
Hpy99I CGWCG 3 cut(s) 65, 130, 545
HpyAV CCTTC 3 cut(s) 148, 695, 786
HpyCH4III ACNGT 2 cut(s) 83, 208
HpyCH4IV ACGT 1 cut(s) 441
HpyCH4V TGCA 6 cut(s) 426, 689, 860, 899, 1025, 1085
HpyF10VI GCNNNNNNNGC 4 cut(s) 481, 503, 695, 1239
HpyF3I CTNAG 4 cut(s) 174, 223, 405, 968
HpySE526I ACGT 1 cut(s) 441
Hsp92I GRCGYC 2 cut(s) 66, 168
Hsp92II CATG 5 cut(s) 193, 274, 380, 1144, 1178
KpnI GGTACC 1 cut(s) 48
KroI GCCGGC 1 cut(s) 5
KroNI GCCGGC 1 cut(s) 7
Kzo9I GATC 6 cut(s) 37, 90, 159, 219, 226, 281
LmnI GCTCC 3 cut(s) 431, 551, 885
Lsp1109I GCAGC 3 cut(s) 438, 446, 1124
LweI GCATC 2 cut(s) 622, 1114
MabI ACCWGGT 1 cut(s) 180
MaeI CTAG 4 cut(s) 522, 855, 920, 938
MaeII ACGT 1 cut(s) 441
MaeIII GTNAC 5 cut(s) 316, 745, 821, 940, 994
MalI GATC 6 cut(s) 39, 92, 161, 221, 228, 283
MboI GATC 6 cut(s) 37, 90, 159, 219, 226, 281
MboII GAAGA 3 cut(s) 664, 846, 1113
MfeI CAATTG 1 cut(s) 357
MflI RGATCY 2 cut(s) 219, 226
MhlI GDGCHC 1 cut(s) 648
MluCI AATT 7 cut(s) 357, 381, 724, 796, 928, 1000, 1184
MlyI GAGTC 1 cut(s) 812
MnlI CCTC 9 cut(s) 10, 147, 337, 474, 523, 596, 602, 977, 1097
MroNI GCCGGC 1 cut(s) 5
MseI TTAA 5 cut(s) 468, 738, 1053, 1161, 1252
MslI CAYNNNNRTG 2 cut(s) 81, 275
MspA1I CMGCKG 1 cut(s) 429
MspI CCGG 5 cut(s) 6, 23, 115, 392, 819
MspR9I CCNGG 2 cut(s) 107, 182
MunI CAATTG 1 cut(s) 357
MvaI CCWGG 2 cut(s) 107, 182
MvnI CGCG 1 cut(s) 867
MwoI GCNNNNNNNGC 4 cut(s) 481, 503, 695, 1239
NaeI GCCGGC 1 cut(s) 7
NdeII GATC 6 cut(s) 37, 90, 159, 219, 226, 281
NgoMIV GCCGGC 1 cut(s) 5
NlaIII CATG 5 cut(s) 193, 274, 380, 1144, 1178
NlaIV GGNNCC 3 cut(s) 21, 46, 547
NmeAIII GCCGAG 1 cut(s) 567
NmuCI GTSAC 1 cut(s) 821
OliI CACNNNNGTG 1 cut(s) 81
PaqCI CACCTGC 1 cut(s) 620
PcsI WCGNNNNNNNCGW 2 cut(s) 125, 141
PdiI GCCGGC 1 cut(s) 7
PfeI GAWTC 2 cut(s) 705, 770
PflFI GACNNNGTC 1 cut(s) 124
PinAI ACCGGT 1 cut(s) 818
PkrI GCNGC 4 cut(s) 172, 428, 436, 1114
PleI GAGTC 1 cut(s) 811
PpsI GAGTC 1 cut(s) 811
Ppu21I YACGTR 1 cut(s) 442
PpuMI RGGWCCY 1 cut(s) 659
PshBI ATTAAT 1 cut(s) 738
Psp5II RGGWCCY 1 cut(s) 659
Psp6I CCWGG 2 cut(s) 105, 180
PspGI CCWGG 2 cut(s) 105, 180
PspN4I GGNNCC 3 cut(s) 21, 46, 547
PspPI GGNCC 4 cut(s) 20, 401, 659, 986
PspPPI RGGWCCY 1 cut(s) 659
PstI CTGCAG 1 cut(s) 428
PsuI RGATCY 2 cut(s) 219, 226
PsyI GACNNNGTC 1 cut(s) 124
RsaI GTAC 1 cut(s) 46
RsaNI GTAC 1 cut(s) 45
RseI CAYNNNNRTG 2 cut(s) 81, 275
SaqAI TTAA 5 cut(s) 468, 738, 1053, 1161, 1252
SatI GCNGC 4 cut(s) 171, 427, 435, 1113
Sau3AI GATC 6 cut(s) 37, 90, 159, 219, 226, 281
Sau96I GGNCC 4 cut(s) 20, 401, 659, 986
SchI GAGTC 1 cut(s) 812
ScrFI CCNGG 2 cut(s) 107, 182
SduI GDGCHC 1 cut(s) 648
SexAI ACCWGGT 1 cut(s) 180
SfaNI GCATC 2 cut(s) 622, 1114
SfcI CTRYAG 1 cut(s) 424
SgrAI CRCCGGYG 1 cut(s) 818
SinI GGWCC 3 cut(s) 20, 659, 986
SmiMI CAYNNNNRTG 2 cut(s) 81, 275
SmlI CTYRAG 2 cut(s) 339, 593
SmoI CTYRAG 2 cut(s) 339, 593
Sse9I AATT 7 cut(s) 357, 381, 724, 796, 928, 1000, 1184
SsiI CCGC 7 cut(s) 171, 429, 452, 460, 497, 669, 865
SspI AATATT 1 cut(s) 1051
SspMI CTAG 4 cut(s) 522, 855, 920, 938
StyD4I CCNGG 2 cut(s) 105, 180
StyI CCWWGG 1 cut(s) 1149
TaaI ACNGT 2 cut(s) 83, 208
TaiI ACGT 1 cut(s) 444
TaqI TCGA 4 cut(s) 36, 982, 1122, 1211
TasI AATT 7 cut(s) 357, 381, 724, 796, 928, 1000, 1184
TauI GCSGC 1 cut(s) 173
TfiI GAWTC 2 cut(s) 705, 770
Tru1I TTAA 5 cut(s) 468, 738, 1053, 1161, 1252
Tru9I TTAA 5 cut(s) 468, 738, 1053, 1161, 1252
TscAI CASTG 2 cut(s) 211, 772
TseFI GTSAC 1 cut(s) 821
TseI GCWGC 3 cut(s) 426, 434, 1112
Tsp45I GTSAC 1 cut(s) 821
TspDTI ATGAA 4 cut(s) 206, 393, 1129, 1178
TspGWI ACGGA 2 cut(s) 872, 1083
TspRI CASTG 2 cut(s) 211, 772
Tth111I GACNNNGTC 1 cut(s) 124
VpaK11BI GGWCC 3 cut(s) 20, 659, 986
VspI ATTAAT 1 cut(s) 738
XapI RAATTY 3 cut(s) 381, 724, 1184
XspI CTAG 4 cut(s) 522, 855, 920, 938
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.