RLG00000030553

strictosidine

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0000674
Physical Location & Seq
Reverse (-)
63426680 .. 63427651
972 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000030553

Sequence Viewer

Length: 972 bp
ATGAAATCAGCTCTTGTTATTCTCTTCTTCTGTTCGTCTGTTCACCAATCCCAAGGAGCATCTTTTTCACTCCCAAACCTAAACCATCATGAAATACCCATCCATGTACCACAGCCCGAAAGCATTGCTTTCGACTGCAATGGCGCACCCTACGTGGGTGTGGCTGATGGCAGGATATTCAAGTGGCTAGGGTACCCTTTTGGGTGGAGAGAATTCGCAGTCACCGCTCCCAATAGGCCGAGAGGAGTATGTGATTTAACAACCAACAAAGACAATGAACCGATATGCGGGAGGCCACTTGGTTTAAAATTTGACCTCACAACATGTGAACTGTATATCGCAGATGCCTCTTTTGGACTTCTAAAGACAGGACCAAATGGTGGAGTAGCCCAAATACTTGCGACATCTGCTGAAGGAGTTCCTTTCCGTTTCTTAAACGGTGTCGACATTGATCGCCAAAGTGGAATGGTGTACTTCACAGACACCAGTGCTATTTACCAGAGAAGAAATTGGGAATTGTCAGTTAGTACAGGTGATAAAACTGGCAGGTTAATGCGTTATGATCCATGGACAAAGAATCTGACTGTGTTGCGTGATGACTTGTCTTTTGCAAATGGGGTGGCACTAAGCAAAGACGGTGATTTTGTGCTAGCAACCCAAACCACCGCAAAGAACATTTTGAGGTACTGGCTCCGAGGGCCTAGAGCCAACACTGTTGATATTTTTTTCCAACTGCAAGGAGCCCCAGACAATATCATCCGAAACATAAATGGAGAATTTTGGGTTGCGGAAAATAGTGCCGGAAAAGGTAAAGCGGTTAGGCTTGACGCTCAAGGAAATTTTCTGGACGTGGTAAATGGACTCAACCCGGTGAGTCATGTTGAGCAGCGCTATAGAAATCTTTGGATAGGATCTGTGGTTAATAACTTTGATATATATTCAAGAAACTTAATTAGAGTTACATCATTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Str_synth

Protein Analysis

324

Amino Acids

35.87

Weight (kDa)

8.24

Isoelectric Point (pI)

27.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Str_synth PF03088 146 - 233 4e-29 Strictosidine synthase
SGL PF08450 177 - 285 8e-11 SMP-30/Gluconolactonase/LRE-like region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 537
Acc65I GGTACC 1 cut(s) 192
AccB1I GGYRCC 1 cut(s) 192
AccB7I CCANNNNNTGG 1 cut(s) 380
AccBSI CCGCTC 1 cut(s) 227
AccI GTMKAC 1 cut(s) 444
AciI CCGC 5 cut(s) 225, 288, 666, 788, 815
AclWI GGATC 2 cut(s) 557, 919
AcsI RAATTY 4 cut(s) 212, 308, 776, 838
AcuI CTGAAG 1 cut(s) 432
AfaI GTAC 5 cut(s) 108, 194, 473, 529, 686
AfeI AGCGCT 1 cut(s) 890
AfiI CCNNNNNNNGG 3 cut(s) 155, 287, 380
AflIII ACRYGT 1 cut(s) 323
AgsI TTSAA 2 cut(s) 181, 942
AjiI CACGTC 1 cut(s) 850
AluBI AGCT 1 cut(s) 11
AluI AGCT 1 cut(s) 11
AlwI GGATC 2 cut(s) 557, 919
Aor51HI AGCGCT 1 cut(s) 890
AoxI GGCC 3 cut(s) 236, 293, 698
ApeKI GCWGC 1 cut(s) 886
ApoI RAATTY 4 cut(s) 212, 308, 776, 838
ArsI GACNNNNNNTTYG 4 cut(s) 590, 622, 626, 658
Asp700I GAANNNNTTC 1 cut(s) 417
Asp718I GGTACC 1 cut(s) 192
AspLEI GCGC 2 cut(s) 146, 891
AspS9I GGNCC 2 cut(s) 371, 698
AsuC2I CCSGG 1 cut(s) 869
AsuHPI GGTGA 5 cut(s) 35, 214, 545, 650, 883
AsuNHI GCTAGC 1 cut(s) 649
AvaII GGWCC 1 cut(s) 371
BanI GGYRCC 1 cut(s) 192
BanII GRGCYC 1 cut(s) 745
BbvI GCAGC 1 cut(s) 898
BccI CCATC 3 cut(s) 93, 107, 161
BcgI CGANNNNNNTGC 4 cut(s) 107, 112, 141, 146
BcnI CCSGG 1 cut(s) 869
BfaI CTAG 3 cut(s) 188, 650, 702
BfmI CTRYAG 1 cut(s) 892
BfoI RGCGCY 1 cut(s) 892
BfuAI ACCTGC 1 cut(s) 537
BisI GCNGC 1 cut(s) 887
BlsI GCNGC 1 cut(s) 888
Bme1390I CCNGG 1 cut(s) 869
Bme18I GGWCC 1 cut(s) 371
BmgBI CACGTC 1 cut(s) 850
BmgT120I GGNCC 2 cut(s) 371, 698
BmiI GGNNCC 3 cut(s) 194, 692, 742
BmrFI CCNGG 1 cut(s) 869
BmsI GCATC 2 cut(s) 68, 334
BmtI GCTAGC 1 cut(s) 653
BpuEI CTTGAG 1 cut(s) 816
BpuMI CCSGG 1 cut(s) 869
BsaAI YACGTR 1 cut(s) 154
BsaJI CCNNGG 3 cut(s) 52, 566, 694
Bsc4I CCNNNNNNNGG 3 cut(s) 155, 287, 380
Bse1I ACTGG 3 cut(s) 486, 547, 692
Bse3DI GCAATG 2 cut(s) 123, 145
BseDI CCNNGG 3 cut(s) 52, 566, 694
BseGI GGATG 2 cut(s) 99, 756
BseLI CCNNNNNNNGG 3 cut(s) 155, 287, 380
BseMI GCAATG 2 cut(s) 123, 145
BseNI ACTGG 3 cut(s) 486, 547, 692
BseRI GAGGAG 1 cut(s) 258
BseXI GCAGC 1 cut(s) 898
BshFI GGCC 3 cut(s) 238, 295, 700
BshNI GGYRCC 1 cut(s) 192
BsiSI CCGG 2 cut(s) 801, 869
BslI CCNNNNNNNGG 3 cut(s) 155, 287, 380
BsnI GGCC 3 cut(s) 238, 295, 700
Bsp1286I GDGCHC 1 cut(s) 745
Bsp143I GATC 3 cut(s) 451, 562, 911
Bsp19I CCATGG 1 cut(s) 566
BspACI CCGC 5 cut(s) 225, 288, 666, 788, 815
BspANI GGCC 3 cut(s) 238, 295, 700
BspHI TCATGA 1 cut(s) 88
BspLI GGNNCC 3 cut(s) 194, 692, 742
BspMI ACCTGC 1 cut(s) 537
BspOI GCTAGC 1 cut(s) 653
BspPI GGATC 2 cut(s) 557, 919
BspT107I GGYRCC 1 cut(s) 192
BsrBI CCGCTC 1 cut(s) 227
BsrDI GCAATG 2 cut(s) 123, 145
BsrI ACTGG 3 cut(s) 486, 547, 692
BssECI CCNNGG 3 cut(s) 52, 566, 694
BssMI GATC 3 cut(s) 451, 562, 911
BssT1I CCWWGG 2 cut(s) 52, 566
Bst4CI ACNGT 5 cut(s) 333, 440, 586, 638, 715
Bst6I CTCTTC 1 cut(s) 29
BstBAI YACGTR 1 cut(s) 154
BstC8I GCNNGC 1 cut(s) 651
BstDEI CTNAG 1 cut(s) 626
BstDSI CCRYGG 1 cut(s) 566
BstF5I GGATG 2 cut(s) 99, 756
BstH2I RGCGCY 1 cut(s) 892
BstHHI GCGC 2 cut(s) 146, 891
BstKTI GATC 3 cut(s) 454, 565, 914
BstMBI GATC 3 cut(s) 451, 562, 911
BstMWI GCNNNNNNNGC 3 cut(s) 224, 407, 697
BstNSI RCATGY 1 cut(s) 327
BstSCI CCNGG 1 cut(s) 867
BstSFI CTRYAG 1 cut(s) 892
BstV1I GCAGC 1 cut(s) 898
BstX2I RGATCY 1 cut(s) 911
BstYI RGATCY 1 cut(s) 911
BsuRI GGCC 3 cut(s) 238, 295, 700
BtgI CCRYGG 1 cut(s) 566
BtrI CACGTC 1 cut(s) 850
BtsCI GGATG 2 cut(s) 99, 756
BtsIMutI CAGTG 2 cut(s) 493, 711
BveI ACCTGC 1 cut(s) 537
Cac8I GCNNGC 1 cut(s) 651
CciI TCATGA 1 cut(s) 88
CfoI GCGC 2 cut(s) 146, 891
Cfr13I GGNCC 2 cut(s) 371, 698
CseI GACGC 1 cut(s) 836
Csp6I GTAC 5 cut(s) 107, 193, 472, 528, 685
CspCI CAANNNNNGTGG 2 cut(s) 600, 635
CviAII CATG 5 cut(s) 89, 104, 324, 567, 878
CviQI GTAC 5 cut(s) 107, 193, 472, 528, 685
DdeI CTNAG 1 cut(s) 626
DpnI GATC 3 cut(s) 453, 564, 913
DpnII GATC 3 cut(s) 451, 562, 911
DraI TTTAAA 1 cut(s) 306
Eam1104I CTCTTC 1 cut(s) 29
EarI CTCTTC 1 cut(s) 29
Eco130I CCWWGG 2 cut(s) 52, 566
Eco24I GRGCYC 1 cut(s) 745
Eco47I GGWCC 1 cut(s) 371
Eco47III AGCGCT 1 cut(s) 890
Eco57I CTGAAG 1 cut(s) 432
EcoO109I RGGNCCY 1 cut(s) 698
EcoRI GAATTC 1 cut(s) 212
EcoT14I CCWWGG 2 cut(s) 52, 566
EcoT38I GRGCYC 1 cut(s) 745
ErhI CCWWGG 2 cut(s) 52, 566
FaeI CATG 5 cut(s) 92, 107, 327, 570, 881
FalI AAGNNNNNCTT 2 cut(s) 112, 144
FatI CATG 5 cut(s) 88, 103, 323, 566, 877
FauI CCCGC 1 cut(s) 281
FblI GTMKAC 1 cut(s) 444
Fnu4HI GCNGC 1 cut(s) 887
FokI GGATG 2 cut(s) 86, 743
FriOI GRGCYC 1 cut(s) 745
Fsp4HI GCNGC 1 cut(s) 887
FspBI CTAG 3 cut(s) 188, 650, 702
GlaI GCGC 2 cut(s) 145, 890
GluI GCNGC 1 cut(s) 887
HaeII RGCGCY 1 cut(s) 892
HaeIII GGCC 3 cut(s) 238, 295, 700
HapII CCGG 2 cut(s) 801, 869
HgaI GACGC 1 cut(s) 836
HhaI GCGC 2 cut(s) 146, 891
Hin1II CATG 5 cut(s) 92, 107, 327, 570, 881
Hin6I GCGC 2 cut(s) 144, 889
HinP1I GCGC 2 cut(s) 144, 889
HincII GTYRAC 1 cut(s) 445
HindII GTYRAC 1 cut(s) 445
HinfI GANTC 3 cut(s) 577, 861, 874
HpaII CCGG 2 cut(s) 801, 869
HphI GGTGA 5 cut(s) 35, 214, 545, 650, 883
Hpy166II GTNNAC 4 cut(s) 43, 329, 445, 472
Hpy188I TCNGA 3 cut(s) 582, 695, 761
Hpy188III TCNNGA 3 cut(s) 89, 845, 942
Hpy8I GTNNAC 4 cut(s) 43, 329, 445, 472
HpyAV CCTTC 1 cut(s) 407
HpyCH4III ACNGT 5 cut(s) 333, 440, 586, 638, 715
HpyCH4IV ACGT 2 cut(s) 153, 849
HpyCH4V TGCA 3 cut(s) 138, 611, 736
HpyF10VI GCNNNNNNNGC 3 cut(s) 224, 407, 697
HpyF3I CTNAG 1 cut(s) 626
HpySE526I ACGT 2 cut(s) 153, 849
Hsp92II CATG 5 cut(s) 92, 107, 327, 570, 881
HspAI GCGC 2 cut(s) 144, 889
KpnI GGTACC 1 cut(s) 196
Kzo9I GATC 3 cut(s) 451, 562, 911
LmnI GCTCC 4 cut(s) 56, 232, 696, 740
Lsp1109I GCAGC 1 cut(s) 898
LweI GCATC 2 cut(s) 68, 334
MaeI CTAG 3 cut(s) 188, 650, 702
MaeII ACGT 2 cut(s) 153, 849
MaeIII GTNAC 2 cut(s) 220, 958
MalI GATC 3 cut(s) 453, 564, 913
MbiI CCGCTC 1 cut(s) 227
MboI GATC 3 cut(s) 451, 562, 911
MboII GAAGA 3 cut(s) 16, 19, 516
MflI RGATCY 1 cut(s) 911
MhlI GDGCHC 1 cut(s) 745
MluCI AATT 7 cut(s) 212, 308, 508, 515, 776, 838, 951
MlyI GAGTC 2 cut(s) 855, 883
MmeI TCCRAC 1 cut(s) 754
MnlI CCTC 6 cut(s) 236, 285, 326, 358, 675, 689
MroXI GAANNNNTTC 1 cut(s) 417
MseI TTAA 6 cut(s) 257, 305, 434, 551, 921, 950
MspI CCGG 2 cut(s) 801, 869
MspR9I CCNGG 1 cut(s) 869
MwoI GCNNNNNNNGC 3 cut(s) 224, 407, 697
NciI CCSGG 1 cut(s) 869
NcoI CCATGG 1 cut(s) 566
NdeII GATC 3 cut(s) 451, 562, 911
NheI GCTAGC 1 cut(s) 649
NlaIII CATG 5 cut(s) 92, 107, 327, 570, 881
NlaIV GGNNCC 3 cut(s) 194, 692, 742
NmeAIII GCCGAG 1 cut(s) 264
NmuCI GTSAC 1 cut(s) 220
NspI RCATGY 1 cut(s) 327
PagI TCATGA 1 cut(s) 88
PciI ACATGT 1 cut(s) 323
PdmI GAANNNNTTC 1 cut(s) 417
PfeI GAWTC 1 cut(s) 577
PflMI CCANNNNNTGG 1 cut(s) 380
PkrI GCNGC 1 cut(s) 888
PleI GAGTC 2 cut(s) 855, 882
PpsI GAGTC 2 cut(s) 855, 882
Ppu21I YACGTR 1 cut(s) 154
PscI ACATGT 1 cut(s) 323
PspN4I GGNNCC 3 cut(s) 194, 692, 742
PspPI GGNCC 2 cut(s) 371, 698
PsuI RGATCY 1 cut(s) 911
RsaI GTAC 5 cut(s) 108, 194, 473, 529, 686
RsaNI GTAC 5 cut(s) 107, 193, 472, 528, 685
SalI GTCGAC 1 cut(s) 443
SaqAI TTAA 6 cut(s) 257, 305, 434, 551, 921, 950
SatI GCNGC 1 cut(s) 887
Sau3AI GATC 3 cut(s) 451, 562, 911
Sau96I GGNCC 2 cut(s) 371, 698
SchI GAGTC 2 cut(s) 855, 883
ScrFI CCNGG 1 cut(s) 869
SduI GDGCHC 1 cut(s) 745
SetI ASST 9 cut(s) 13, 81, 156, 318, 535, 551, 686, 811, 852
SfaNI GCATC 2 cut(s) 68, 334
SfcI CTRYAG 1 cut(s) 892
SinI GGWCC 1 cut(s) 371
SmlI CTYRAG 1 cut(s) 831
SmoI CTYRAG 1 cut(s) 831
Sse9I AATT 7 cut(s) 212, 308, 508, 515, 776, 838, 951
SsiI CCGC 5 cut(s) 225, 288, 666, 788, 815
SspMI CTAG 3 cut(s) 188, 650, 702
StyD4I CCNGG 1 cut(s) 867
StyI CCWWGG 2 cut(s) 52, 566
TaaI ACNGT 5 cut(s) 333, 440, 586, 638, 715
TaiI ACGT 2 cut(s) 156, 852
TaqI TCGA 2 cut(s) 132, 444
TasI AATT 7 cut(s) 212, 308, 508, 515, 776, 838, 951
TatI WGTACW 2 cut(s) 471, 527
TfiI GAWTC 1 cut(s) 577
Tru1I TTAA 6 cut(s) 257, 305, 434, 551, 921, 950
Tru9I TTAA 6 cut(s) 257, 305, 434, 551, 921, 950
TscAI CASTG 2 cut(s) 493, 718
TseFI GTSAC 1 cut(s) 220
TseI GCWGC 1 cut(s) 886
Tsp45I GTSAC 1 cut(s) 220
TspDTI ATGAA 3 cut(s) 17, 105, 291
TspGWI ACGGA 1 cut(s) 416
TspRI CASTG 2 cut(s) 493, 718
Van91I CCANNNNNTGG 1 cut(s) 380
VpaK11BI GGWCC 1 cut(s) 371
XapI RAATTY 4 cut(s) 212, 308, 776, 838
XceI RCATGY 1 cut(s) 327
XmiI GTMKAC 1 cut(s) 444
XmnI GAANNNNTTC 1 cut(s) 417
XspI CTAG 3 cut(s) 188, 650, 702
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.