Rh1BG027400

strictosidine

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1B
OG0000674
Physical Location & Seq
Forward (+)
3848560 .. 3850366
1807 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1BG027400.1

Sequence Viewer

Length: 552 bp
ATGGTGTACTTCACGGACACCAGTGCTATTTACCAGAGAAGAAATTGGGAATTGTCAGTTAGTACAGGTGATAAAACTGGAAGGTTAATGCGATATGATCCATGGACAAAGAATGTCACTGTGTTGCGTCATGACTTGTCTTTTGCAAACGGGGTGGCACTAAGCAAAGACGGTGATTTTGTGCTAGTAACCCAAACCACTGCAAAGAACATTTTGAGGTATTGGCTTCGAGGGCCTAGAGCCAACACTGTTGATATTTTTTTCCAACTGCAAGGAGCCCCGGACAATATCATCAGAAACATAAATGGAGAATTTTGGGTTGCAGAAAATAGTGCCGGAAAAGGTAAAGCGGTCAAGCTTGACGCTCAAGGAAACTTTCTAGACGTGGTGGATGGACTCAACCCTGTGAGTCATGTTGAGCAGCGTTATAGAAACCTTTGGATAGGATCTGTGTTGCTTACACCTTTTCCTACTTCCTTTCGTTCTCTCTTTGTCCCAGAGGCTTTATCAAAACACATGATAGCTAGGGTTCCGAGAACATATATACTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Str_synth

Protein Analysis

183

Amino Acids

20.79

Weight (kDa)

9.73

Isoelectric Point (pI)

25.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Str_synth PF03088 2 - 78 7.1e-27 Strictosidine synthase
SGL PF08450 22 - 131 7.6e-11 SMP-30/Gluconolactonase/LRE-like region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 350
AclWI GGATC 2 cut(s) 92, 454
AcsI RAATTY 1 cut(s) 311
AfaI GTAC 2 cut(s) 8, 64
AjiI CACGTC 1 cut(s) 385
AluBI AGCT 2 cut(s) 358, 524
AluI AGCT 2 cut(s) 358, 524
AlwI GGATC 2 cut(s) 92, 454
AoxI GGCC 1 cut(s) 233
ApeKI GCWGC 1 cut(s) 421
ApoI RAATTY 1 cut(s) 311
ArsI GACNNNNNNTTYG 4 cut(s) 125, 157, 161, 193
AspS9I GGNCC 1 cut(s) 233
AsuC2I CCSGG 1 cut(s) 281
AsuHPI GGTGA 2 cut(s) 80, 185
BanII GRGCYC 1 cut(s) 280
BbvI GCAGC 1 cut(s) 433
BccI CCATC 1 cut(s) 386
BcnI CCSGG 1 cut(s) 281
BfaI CTAG 4 cut(s) 185, 237, 380, 525
BisI GCNGC 1 cut(s) 422
BlsI GCNGC 1 cut(s) 423
Bme1390I CCNGG 1 cut(s) 281
BmgBI CACGTC 1 cut(s) 385
BmgT120I GGNCC 1 cut(s) 233
BmiI GGNNCC 2 cut(s) 277, 531
BmrFI CCNGG 1 cut(s) 281
BpuEI CTTGAG 1 cut(s) 351
BpuMI CCSGG 1 cut(s) 281
BsaJI CCNNGG 2 cut(s) 101, 279
Bse1I ACTGG 2 cut(s) 21, 82
BseDI CCNNGG 2 cut(s) 101, 279
BseGI GGATG 1 cut(s) 397
BseNI ACTGG 2 cut(s) 21, 82
BseXI GCAGC 1 cut(s) 433
BshFI GGCC 1 cut(s) 235
BsiSI CCGG 2 cut(s) 281, 336
BslFI GGGAC 1 cut(s) 479
BsmFI GGGAC 1 cut(s) 479
BsnI GGCC 1 cut(s) 235
Bsp1286I GDGCHC 1 cut(s) 280
Bsp143I GATC 2 cut(s) 97, 446
Bsp19I CCATGG 1 cut(s) 101
BspACI CCGC 1 cut(s) 350
BspANI GGCC 1 cut(s) 235
BspHI TCATGA 1 cut(s) 130
BspLI GGNNCC 2 cut(s) 277, 531
BspPI GGATC 2 cut(s) 92, 454
BsrI ACTGG 2 cut(s) 21, 82
BssECI CCNNGG 2 cut(s) 101, 279
BssMI GATC 2 cut(s) 97, 446
BssT1I CCWWGG 1 cut(s) 101
Bst4CI ACNGT 3 cut(s) 121, 173, 250
BstDEI CTNAG 1 cut(s) 161
BstDSI CCRYGG 1 cut(s) 101
BstF5I GGATG 1 cut(s) 397
BstKTI GATC 2 cut(s) 100, 449
BstMBI GATC 2 cut(s) 97, 446
BstMWI GCNNNNNNNGC 1 cut(s) 232
BstSCI CCNGG 1 cut(s) 279
BstV1I GCAGC 1 cut(s) 433
BstX2I RGATCY 1 cut(s) 446
BstYI RGATCY 1 cut(s) 446
BsuRI GGCC 1 cut(s) 235
BtgI CCRYGG 1 cut(s) 101
BtrI CACGTC 1 cut(s) 385
BtsCI GGATG 1 cut(s) 397
BtsI GCAGTG 1 cut(s) 198
BtsIMutI CAGTG 4 cut(s) 28, 117, 198, 246
CciI TCATGA 1 cut(s) 130
Cfr13I GGNCC 1 cut(s) 233
CseI GACGC 2 cut(s) 116, 371
Csp6I GTAC 2 cut(s) 7, 63
CspCI CAANNNNNGTGG 2 cut(s) 135, 170
CviAII CATG 4 cut(s) 102, 131, 413, 517
CviJI RGCY 7 cut(s) 226, 235, 242, 278, 358, 503, 524
CviKI_1 RGCY 7 cut(s) 226, 235, 242, 278, 358, 503, 524
CviQI GTAC 2 cut(s) 7, 63
DdeI CTNAG 1 cut(s) 161
DpnI GATC 2 cut(s) 99, 448
DpnII GATC 2 cut(s) 97, 446
Eco130I CCWWGG 1 cut(s) 101
Eco24I GRGCYC 1 cut(s) 280
EcoO109I RGGNCCY 1 cut(s) 233
EcoT14I CCWWGG 1 cut(s) 101
EcoT38I GRGCYC 1 cut(s) 280
ErhI CCWWGG 1 cut(s) 101
FaeI CATG 4 cut(s) 105, 134, 416, 520
FaqI GGGAC 1 cut(s) 479
FatI CATG 4 cut(s) 101, 130, 412, 516
Fnu4HI GCNGC 1 cut(s) 422
FokI GGATG 1 cut(s) 404
FriOI GRGCYC 1 cut(s) 280
Fsp4HI GCNGC 1 cut(s) 422
FspBI CTAG 4 cut(s) 185, 237, 380, 525
GluI GCNGC 1 cut(s) 422
HaeIII GGCC 1 cut(s) 235
HapII CCGG 2 cut(s) 281, 336
HgaI GACGC 2 cut(s) 116, 371
Hin1II CATG 4 cut(s) 105, 134, 416, 520
HindIII AAGCTT 1 cut(s) 356
HinfI GANTC 2 cut(s) 396, 409
HpaII CCGG 2 cut(s) 281, 336
HphI GGTGA 2 cut(s) 80, 185
Hpy166II GTNNAC 1 cut(s) 7
Hpy188I TCNGA 2 cut(s) 296, 534
Hpy188III TCNNGA 2 cut(s) 131, 380
Hpy8I GTNNAC 1 cut(s) 7
HpyAV CCTTC 1 cut(s) 75
HpyCH4III ACNGT 3 cut(s) 121, 173, 250
HpyCH4IV ACGT 1 cut(s) 384
HpyCH4V TGCA 4 cut(s) 146, 203, 271, 323
HpyF10VI GCNNNNNNNGC 1 cut(s) 232
HpyF3I CTNAG 1 cut(s) 161
HpySE526I ACGT 1 cut(s) 384
Hsp92II CATG 4 cut(s) 105, 134, 416, 520
Kzo9I GATC 2 cut(s) 97, 446
LmnI GCTCC 1 cut(s) 275
LpnPI CCDG 8 cut(s) 34, 47, 51, 63, 294, 349, 417, 510
Lsp1109I GCAGC 1 cut(s) 433
MaeI CTAG 4 cut(s) 185, 237, 380, 525
MaeII ACGT 1 cut(s) 384
MaeIII GTNAC 2 cut(s) 115, 187
MalI GATC 2 cut(s) 99, 448
MboI GATC 2 cut(s) 97, 446
MboII GAAGA 1 cut(s) 51
MflI RGATCY 1 cut(s) 446
MhlI GDGCHC 1 cut(s) 280
MluCI AATT 3 cut(s) 43, 50, 311
MlyI GAGTC 2 cut(s) 390, 418
MmeI TCCRAC 1 cut(s) 289
MnlI CCTC 3 cut(s) 210, 224, 493
MseI TTAA 2 cut(s) 86, 550
MspI CCGG 2 cut(s) 281, 336
MspR9I CCNGG 1 cut(s) 281
MwoI GCNNNNNNNGC 1 cut(s) 232
NciI CCSGG 1 cut(s) 281
NcoI CCATGG 1 cut(s) 101
NdeII GATC 2 cut(s) 97, 446
NlaIII CATG 4 cut(s) 105, 134, 416, 520
NlaIV GGNNCC 2 cut(s) 277, 531
NmuCI GTSAC 1 cut(s) 115
PagI TCATGA 1 cut(s) 130
PkrI GCNGC 1 cut(s) 423
PleI GAGTC 2 cut(s) 390, 417
PpsI GAGTC 2 cut(s) 390, 417
PspN4I GGNNCC 2 cut(s) 277, 531
PspPI GGNCC 1 cut(s) 233
PsuI RGATCY 1 cut(s) 446
RsaI GTAC 2 cut(s) 8, 64
RsaNI GTAC 2 cut(s) 7, 63
SaqAI TTAA 2 cut(s) 86, 550
SatI GCNGC 1 cut(s) 422
Sau3AI GATC 2 cut(s) 97, 446
Sau96I GGNCC 1 cut(s) 233
SchI GAGTC 2 cut(s) 390, 418
ScrFI CCNGG 1 cut(s) 281
SduI GDGCHC 1 cut(s) 280
SetI ASST 9 cut(s) 70, 86, 221, 346, 360, 387, 438, 466, 526
SmlI CTYRAG 1 cut(s) 366
SmoI CTYRAG 1 cut(s) 366
Sse9I AATT 3 cut(s) 43, 50, 311
SsiI CCGC 1 cut(s) 350
SspMI CTAG 4 cut(s) 185, 237, 380, 525
StyD4I CCNGG 1 cut(s) 279
StyI CCWWGG 1 cut(s) 101
TaaI ACNGT 3 cut(s) 121, 173, 250
TaiI ACGT 1 cut(s) 387
TaqI TCGA 1 cut(s) 229
TasI AATT 3 cut(s) 43, 50, 311
TatI WGTACW 2 cut(s) 6, 62
Tru1I TTAA 2 cut(s) 86, 550
Tru9I TTAA 2 cut(s) 86, 550
TscAI CASTG 4 cut(s) 28, 124, 205, 253
TseFI GTSAC 1 cut(s) 115
TseI GCWGC 1 cut(s) 421
Tsp45I GTSAC 1 cut(s) 115
TspGWI ACGGA 1 cut(s) 29
TspRI CASTG 4 cut(s) 28, 124, 205, 253
XapI RAATTY 1 cut(s) 311
XbaI TCTAGA 1 cut(s) 379
XspI CTAG 4 cut(s) 185, 237, 380, 525
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.