RchiOBHm_Chr1g0318261

strictosidine

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0000674
Physical Location & Seq
Forward (+)
5730353 .. 5731324
972 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ54855

Sequence Viewer

Length: 972 bp
ATGAAATCAGCTCTTGTTACACTCTTCTTCTTCTTCTTCTTCCTCTTTTTGTTTGTTCACCGAGCCCAAGGAGCATCTTTTTCACTCCCAAACCTAAACCATCATGAAATACCCATCCATGTACCGCAGCCCGAAAGCATTGCTTTCGACTGCAATGGCGCACCCTACGTGGGTGTGGCTGATGGCAGGATATTCAAGTGGCTAGGATACCCTTTTGGGTGGAGAGAATTCGCAGTCACCGCCTCCAATAGGCCGAGAGGAGTATGTGATTTGACAACCAACAAAGACAATGAACCGATATGCGGGAGGCCACTTGGTTTAAAATTCGACCTCACAACATGTGAACTGTATATCGCAGATGCCTATTTTGGGCTTCTGAAGACAGGACCAAATGGTGGAGTAGCTCAAATACTTGCGACATCTGCTGAAGGAGTTCCTTTCGGTTTCTTAAACGGTGTCGACATTGATCGCCAAAGTGGAATGGTGTACTTCACAGACACCAGTGCTATTTACCAGAGAAGAAATTGGGAATTGTCAGTTAGTACAGGTGATAAAACTGGAAGGTTAATGCGATATGATCCATGGACAAAGAATGTCACTGTGTTGCGTCATGACTTGTCTTTTGCAAACGGGGTGGCACTAAGCAAAGACGGTGATTTTGTGCTAGTAACCCAAACCACTGCAAAGAACATTTTGAGGTATTGGCTTCGAGGGCCTAGAGCCAACACGGTTGATATTTTTTTCCAACTGCAAGGAGCCCCGGACAATATCATCAGAAACATAAATGGAGAATTTTGGGTTGCAGAAAATAGTGCCGGAAAAGGTAAAGCGGTCAAGCTTGACGCTCAAGGAAACTTTCTAGACGTGGTGGATGGACTCAACCCTGTGAGTCATGTTGAGCAGCGTTATAGAAACCTTTGGATAGGATCTGTGGTTAATAACTTTGTTGGGGTTATTCTTAATTTTGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Str_synth

Protein Analysis

323

Amino Acids

35.99

Weight (kDa)

7.69

Isoelectric Point (pI)

21.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Str_synth PF03088 151 - 238 7.2e-30 Strictosidine synthase
SGL PF08450 182 - 291 3.1e-10 SMP-30/Gluconolactonase/LRE-like region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 395
AccI GTMKAC 1 cut(s) 459
AciI CCGC 4 cut(s) 125, 240, 303, 830
AclWI GGATC 2 cut(s) 572, 934
AcsI RAATTY 3 cut(s) 227, 323, 791
AcuI CTGAAG 2 cut(s) 398, 447
AfaI GTAC 3 cut(s) 123, 488, 544
AfiI CCNNNNNNNGG 5 cut(s) 170, 249, 302, 369, 395
AflIII ACRYGT 1 cut(s) 338
AgsI TTSAA 1 cut(s) 196
AjiI CACGTC 1 cut(s) 865
AluBI AGCT 3 cut(s) 11, 404, 838
AluI AGCT 3 cut(s) 11, 404, 838
AlwI GGATC 2 cut(s) 572, 934
AoxI GGCC 3 cut(s) 251, 308, 713
ApeKI GCWGC 2 cut(s) 127, 901
ApoI RAATTY 3 cut(s) 227, 323, 791
ArsI GACNNNNNNTTYG 4 cut(s) 605, 637, 641, 673
Asp700I GAANNNNTTC 1 cut(s) 432
AspLEI GCGC 1 cut(s) 161
AspS9I GGNCC 2 cut(s) 386, 713
AsuC2I CCSGG 1 cut(s) 761
AsuHPI GGTGA 4 cut(s) 50, 229, 560, 665
AvaII GGWCC 1 cut(s) 386
BanII GRGCYC 2 cut(s) 67, 760
BbsI GAAGAC 1 cut(s) 386
BbvI GCAGC 2 cut(s) 139, 913
BccI CCATC 4 cut(s) 108, 122, 176, 866
BcgI CGANNNNNNTGC 4 cut(s) 122, 127, 156, 161
BciVI GTATCC 1 cut(s) 200
BcnI CCSGG 1 cut(s) 761
BfaI CTAG 4 cut(s) 203, 665, 717, 860
BfuI GTATCC 1 cut(s) 200
BisI GCNGC 2 cut(s) 128, 902
BlsI GCNGC 2 cut(s) 129, 903
Bme1390I CCNGG 1 cut(s) 761
Bme18I GGWCC 1 cut(s) 386
BmgBI CACGTC 1 cut(s) 865
BmgT120I GGNCC 2 cut(s) 386, 713
BmiI GGNNCC 1 cut(s) 757
BmrFI CCNGG 1 cut(s) 761
BmsI GCATC 2 cut(s) 83, 349
BpiI GAAGAC 1 cut(s) 386
BpuEI CTTGAG 1 cut(s) 831
BpuMI CCSGG 1 cut(s) 761
BsaAI YACGTR 1 cut(s) 169
BsaJI CCNNGG 3 cut(s) 67, 581, 759
Bsc4I CCNNNNNNNGG 5 cut(s) 170, 249, 302, 369, 395
Bse1I ACTGG 2 cut(s) 501, 562
Bse3DI GCAATG 2 cut(s) 138, 160
BseDI CCNNGG 3 cut(s) 67, 581, 759
BseGI GGATG 2 cut(s) 114, 877
BseLI CCNNNNNNNGG 5 cut(s) 170, 249, 302, 369, 395
BseMI GCAATG 2 cut(s) 138, 160
BseNI ACTGG 2 cut(s) 501, 562
BseRI GAGGAG 1 cut(s) 273
BseXI GCAGC 2 cut(s) 139, 913
BshFI GGCC 3 cut(s) 253, 310, 715
BsiSI CCGG 2 cut(s) 761, 816
BslI CCNNNNNNNGG 5 cut(s) 170, 249, 302, 369, 395
BsnI GGCC 3 cut(s) 253, 310, 715
Bsp1286I GDGCHC 2 cut(s) 67, 760
Bsp143I GATC 3 cut(s) 466, 577, 926
Bsp19I CCATGG 1 cut(s) 581
BspACI CCGC 4 cut(s) 125, 240, 303, 830
BspANI GGCC 3 cut(s) 253, 310, 715
BspHI TCATGA 2 cut(s) 103, 610
BspLI GGNNCC 1 cut(s) 757
BspPI GGATC 2 cut(s) 572, 934
BsrDI GCAATG 2 cut(s) 138, 160
BsrI ACTGG 2 cut(s) 501, 562
BssECI CCNNGG 3 cut(s) 67, 581, 759
BssMI GATC 3 cut(s) 466, 577, 926
BssT1I CCWWGG 2 cut(s) 67, 581
Bst4CI ACNGT 5 cut(s) 348, 455, 601, 653, 730
Bst6I CTCTTC 1 cut(s) 29
BstBAI YACGTR 1 cut(s) 169
BstDEI CTNAG 1 cut(s) 641
BstDSI CCRYGG 1 cut(s) 581
BstENI CCTNNNNNAGG 1 cut(s) 247
BstF5I GGATG 2 cut(s) 114, 877
BstHHI GCGC 1 cut(s) 161
BstKTI GATC 3 cut(s) 469, 580, 929
BstMBI GATC 3 cut(s) 466, 577, 926
BstMWI GCNNNNNNNGC 4 cut(s) 71, 239, 422, 712
BstNSI RCATGY 1 cut(s) 342
BstSCI CCNGG 1 cut(s) 759
BstV1I GCAGC 2 cut(s) 139, 913
BstV2I GAAGAC 1 cut(s) 386
BstX2I RGATCY 1 cut(s) 926
BstYI RGATCY 1 cut(s) 926
BsuI GTATCC 1 cut(s) 200
BsuRI GGCC 3 cut(s) 253, 310, 715
BtgI CCRYGG 1 cut(s) 581
BtrI CACGTC 1 cut(s) 865
BtsCI GGATG 2 cut(s) 114, 877
BtsI GCAGTG 1 cut(s) 678
BtsIMutI CAGTG 3 cut(s) 508, 597, 678
CciI TCATGA 2 cut(s) 103, 610
CfoI GCGC 1 cut(s) 161
Cfr13I GGNCC 2 cut(s) 386, 713
CseI GACGC 2 cut(s) 596, 851
Csp6I GTAC 3 cut(s) 122, 487, 543
CspCI CAANNNNNGTGG 2 cut(s) 615, 650
CviAII CATG 6 cut(s) 104, 119, 339, 582, 611, 893
CviQI GTAC 3 cut(s) 122, 487, 543
DdeI CTNAG 1 cut(s) 641
DpnI GATC 3 cut(s) 468, 579, 928
DpnII GATC 3 cut(s) 466, 577, 926
DraI TTTAAA 1 cut(s) 321
Eam1104I CTCTTC 1 cut(s) 29
EarI CTCTTC 1 cut(s) 29
Eco130I CCWWGG 2 cut(s) 67, 581
Eco24I GRGCYC 2 cut(s) 67, 760
Eco47I GGWCC 1 cut(s) 386
Eco57I CTGAAG 2 cut(s) 398, 447
EcoNI CCTNNNNNAGG 1 cut(s) 247
EcoO109I RGGNCCY 1 cut(s) 713
EcoRI GAATTC 1 cut(s) 227
EcoT14I CCWWGG 2 cut(s) 67, 581
EcoT38I GRGCYC 2 cut(s) 67, 760
ErhI CCWWGG 2 cut(s) 67, 581
FaeI CATG 6 cut(s) 107, 122, 342, 585, 614, 896
FalI AAGNNNNNCTT 2 cut(s) 127, 159
FatI CATG 6 cut(s) 103, 118, 338, 581, 610, 892
FauI CCCGC 1 cut(s) 296
FblI GTMKAC 1 cut(s) 459
Fnu4HI GCNGC 2 cut(s) 128, 902
FokI GGATG 2 cut(s) 101, 884
FriOI GRGCYC 2 cut(s) 67, 760
Fsp4HI GCNGC 2 cut(s) 128, 902
FspBI CTAG 4 cut(s) 203, 665, 717, 860
GlaI GCGC 1 cut(s) 160
GluI GCNGC 2 cut(s) 128, 902
HaeIII GGCC 3 cut(s) 253, 310, 715
HapII CCGG 2 cut(s) 761, 816
HgaI GACGC 2 cut(s) 596, 851
HhaI GCGC 1 cut(s) 161
Hin1II CATG 6 cut(s) 107, 122, 342, 585, 614, 896
Hin6I GCGC 1 cut(s) 159
HinP1I GCGC 1 cut(s) 159
HincII GTYRAC 1 cut(s) 460
HindII GTYRAC 1 cut(s) 460
HindIII AAGCTT 1 cut(s) 836
HinfI GANTC 2 cut(s) 876, 889
HpaII CCGG 2 cut(s) 761, 816
HphI GGTGA 4 cut(s) 50, 229, 560, 665
Hpy166II GTNNAC 4 cut(s) 58, 344, 460, 487
Hpy188I TCNGA 2 cut(s) 378, 776
Hpy188III TCNNGA 3 cut(s) 104, 611, 860
Hpy8I GTNNAC 4 cut(s) 58, 344, 460, 487
HpyAV CCTTC 2 cut(s) 422, 555
HpyCH4III ACNGT 5 cut(s) 348, 455, 601, 653, 730
HpyCH4IV ACGT 2 cut(s) 168, 864
HpyCH4V TGCA 5 cut(s) 153, 626, 683, 751, 803
HpyF10VI GCNNNNNNNGC 4 cut(s) 71, 239, 422, 712
HpyF3I CTNAG 1 cut(s) 641
HpySE526I ACGT 2 cut(s) 168, 864
Hsp92II CATG 6 cut(s) 107, 122, 342, 585, 614, 896
HspAI GCGC 1 cut(s) 159
Kzo9I GATC 3 cut(s) 466, 577, 926
LmnI GCTCC 2 cut(s) 71, 755
LpnPI CCDG 9 cut(s) 172, 369, 514, 527, 531, 543, 774, 829, 897
Lsp1109I GCAGC 2 cut(s) 139, 913
LweI GCATC 2 cut(s) 83, 349
MaeI CTAG 4 cut(s) 203, 665, 717, 860
MaeII ACGT 2 cut(s) 168, 864
MaeIII GTNAC 4 cut(s) 16, 235, 595, 667
MalI GATC 3 cut(s) 468, 579, 928
MboI GATC 3 cut(s) 466, 577, 926
MboII GAAGA 8 cut(s) 16, 19, 22, 25, 28, 31, 391, 531
MflI RGATCY 1 cut(s) 926
MhlI GDGCHC 2 cut(s) 67, 760
MluCI AATT 6 cut(s) 227, 323, 523, 530, 791, 961
MlyI GAGTC 2 cut(s) 870, 898
MmeI TCCRAC 1 cut(s) 769
MnlI CCTC 7 cut(s) 53, 251, 253, 300, 341, 690, 704
MroXI GAANNNNTTC 1 cut(s) 432
MseI TTAA 5 cut(s) 320, 449, 566, 936, 960
MspI CCGG 2 cut(s) 761, 816
MspR9I CCNGG 1 cut(s) 761
MwoI GCNNNNNNNGC 4 cut(s) 71, 239, 422, 712
NciI CCSGG 1 cut(s) 761
NcoI CCATGG 1 cut(s) 581
NdeII GATC 3 cut(s) 466, 577, 926
NlaIII CATG 6 cut(s) 107, 122, 342, 585, 614, 896
NlaIV GGNNCC 1 cut(s) 757
NmeAIII GCCGAG 1 cut(s) 279
NmuCI GTSAC 2 cut(s) 235, 595
NspI RCATGY 1 cut(s) 342
PagI TCATGA 2 cut(s) 103, 610
PciI ACATGT 1 cut(s) 338
PdmI GAANNNNTTC 1 cut(s) 432
PflMI CCANNNNNTGG 1 cut(s) 395
PkrI GCNGC 2 cut(s) 129, 903
PleI GAGTC 2 cut(s) 870, 897
PpsI GAGTC 2 cut(s) 870, 897
Ppu21I YACGTR 1 cut(s) 169
PscI ACATGT 1 cut(s) 338
PspN4I GGNNCC 1 cut(s) 757
PspPI GGNCC 2 cut(s) 386, 713
PsuI RGATCY 1 cut(s) 926
RsaI GTAC 3 cut(s) 123, 488, 544
RsaNI GTAC 3 cut(s) 122, 487, 543
SalI GTCGAC 1 cut(s) 458
SaqAI TTAA 5 cut(s) 320, 449, 566, 936, 960
SatI GCNGC 2 cut(s) 128, 902
Sau3AI GATC 3 cut(s) 466, 577, 926
Sau96I GGNCC 2 cut(s) 386, 713
SchI GAGTC 2 cut(s) 870, 898
ScrFI CCNGG 1 cut(s) 761
SduI GDGCHC 2 cut(s) 67, 760
SfaNI GCATC 2 cut(s) 83, 349
SinI GGWCC 1 cut(s) 386
SmlI CTYRAG 1 cut(s) 846
SmoI CTYRAG 1 cut(s) 846
Sse9I AATT 6 cut(s) 227, 323, 523, 530, 791, 961
SsiI CCGC 4 cut(s) 125, 240, 303, 830
SspMI CTAG 4 cut(s) 203, 665, 717, 860
StyD4I CCNGG 1 cut(s) 759
StyI CCWWGG 2 cut(s) 67, 581
TaaI ACNGT 5 cut(s) 348, 455, 601, 653, 730
TaiI ACGT 2 cut(s) 171, 867
TaqI TCGA 4 cut(s) 147, 327, 459, 709
TasI AATT 6 cut(s) 227, 323, 523, 530, 791, 961
TatI WGTACW 2 cut(s) 486, 542
Tru1I TTAA 5 cut(s) 320, 449, 566, 936, 960
Tru9I TTAA 5 cut(s) 320, 449, 566, 936, 960
TscAI CASTG 3 cut(s) 508, 604, 685
TseFI GTSAC 2 cut(s) 235, 595
TseI GCWGC 2 cut(s) 127, 901
Tsp45I GTSAC 2 cut(s) 235, 595
TspDTI ATGAA 3 cut(s) 17, 120, 306
TspRI CASTG 3 cut(s) 508, 604, 685
Van91I CCANNNNNTGG 1 cut(s) 395
VpaK11BI GGWCC 1 cut(s) 386
XagI CCTNNNNNAGG 1 cut(s) 247
XapI RAATTY 3 cut(s) 227, 323, 791
XbaI TCTAGA 1 cut(s) 859
XceI RCATGY 1 cut(s) 342
XmiI GTMKAC 1 cut(s) 459
XmnI GAANNNNTTC 1 cut(s) 432
XspI CTAG 4 cut(s) 203, 665, 717, 860
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.