pycom13g04670

strictosidine

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0000674
Physical Location & Seq
Reverse (-)
3115735 .. 3119459
3725 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom13g04670.1

Sequence Viewer

Length: 1074 bp
ATGTGGCCACCTTCATTTCTTTTGTTGTTTTTGCTCCTAACTGTCATAGCTCCTGACGCCATTCTATCATCCAAACCAGCTTCTTGTAAGTCCGTCAACCTTGGAAACTACCGTGAGATAGAGCTGGCTAGGGTTGTTGGCCCTGAAAGCATTGCCTTTGACTGCCGTGGAAAAGGACCCTACGTTGGGGTTTCGGATGGTACGATTCTCAAATGGCAAGGACCTCGCTTGGGTTGGACGGAGTTCGCCTTCACCTCCCCAAACAGGGCAAGGAAACTATGTGATGGTTCAACCAACGTGACGAACGAACCAATATGTGGGAGGCCACTGGGTCTGATGTTCCACCCTAAAACCTGTGATCTCTACTTTGCAGATGCCTACTTCGGGCTGATGAAGACGGGGCCTAATGGTGGTAAGGCGGAAGTTCTTGCCAATTCTATAGGAGGAGTCCCCTTCCTTTTTGTAAACTCTTTGGATATTGATGAAGAAACAGGAATCGTTTATTTCACAGACACAAGCAGTGTGTTCCAAAGAAGGGTATGGGTTCAGTCGGTCCTAGCCGGTGACAAAACTGGCAGGCTACTACAATACGACCCACGTACAAAGAAAGTGAAAGTACTGCTCCACGGTCTGGGTTTTGCAAACGGCGTGGCTCTGAGCGGCGACAAGTCCTTTGTCCTCATCGTCGAATCGGCCGAGTTTAGAATCCACAGGCTATGGCTCCGAGGACCCAAAGCTCATGTGTTGGAACTTTTTATTCAGCTGCAGAGGCCCCCGGATAACATCAAGAGAACAAAAAGAGGAGAGTTTTGGGTTGGGCTCAACAGTGGAAGAGGAGTTAAGGGAGTGCAGAACAAAGTGCAGAATATGATGAATAATAATGAAAGCTCGGGGGAGTTGTTGAATGCGAATGATCCTGTGGGGGTGAAACTTGACGAACAAGGTGGGGTTTTGCAGGTTTTGGATGGAGAGGGTGGTAAAACTTTGCAGGCTGTTAGTGAGGTTGAAGAACACAATGGGCTTTTGTGGATTGGATCAGTGGTGACTTCTTATGTTGGTGTGATCAAGATTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Str_synth

Protein Analysis

358

Amino Acids

39.05

Weight (kDa)

8.7

Isoelectric Point (pI)

29.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Str_synth PF03088 156 - 243 8e-30 Strictosidine synthase
SGL PF08450 189 - 288 4.2e-07 SMP-30/Gluconolactonase/LRE-like region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 946
AccB7I CCANNNNNTGG 2 cut(s) 317, 631
AccBSI CCGCTC 1 cut(s) 660
AciI CCGC 2 cut(s) 419, 660
AclWI GGATC 2 cut(s) 908, 1042
AcoI YGGCCR 2 cut(s) 5, 693
AcyI GRCGYC 1 cut(s) 57
AfaI GTAC 3 cut(s) 202, 601, 618
AgsI TTSAA 3 cut(s) 291, 904, 1007
AluBI AGCT 6 cut(s) 50, 80, 124, 737, 763, 888
AluI AGCT 6 cut(s) 50, 80, 124, 737, 763, 888
AlwI GGATC 2 cut(s) 908, 1042
Ama87I CYCGRG 1 cut(s) 889
AoxI GGCC 6 cut(s) 5, 139, 323, 401, 693, 770
ApeKI GCWGC 1 cut(s) 763
ArsI GACNNNNNNTTYG 2 cut(s) 656, 688
AspS9I GGNCC 7 cut(s) 140, 176, 221, 401, 553, 728, 771
AsuC2I CCSGG 1 cut(s) 776
AsuHPI GGTGA 4 cut(s) 244, 575, 937, 1054
AvaI CYCGRG 1 cut(s) 889
AvaII GGWCC 4 cut(s) 176, 221, 553, 728
BalI TGGCCA 1 cut(s) 7
BanII GRGCYC 1 cut(s) 822
BbsI GAAGAC 1 cut(s) 401
BbvI GCAGC 1 cut(s) 750
BccI CCATC 3 cut(s) 191, 278, 959
BceAI ACGGC 2 cut(s) 150, 661
BcgI CGANNNNNNTGC 2 cut(s) 206, 240
BclI TGATCA 1 cut(s) 1062
BcnI CCSGG 1 cut(s) 776
BfaI CTAG 2 cut(s) 129, 557
BfmI CTRYAG 2 cut(s) 438, 764
BfuAI ACCTGC 1 cut(s) 946
BisI GCNGC 2 cut(s) 661, 764
BlsI GCNGC 2 cut(s) 662, 765
BmcAI AGTACT 1 cut(s) 618
Bme1390I CCNGG 1 cut(s) 776
Bme18I GGWCC 4 cut(s) 176, 221, 553, 728
BmeT110I CYCGRG 1 cut(s) 889
BmgT120I GGNCC 7 cut(s) 140, 176, 221, 401, 553, 728, 771
BmiI GGNNCC 5 cut(s) 178, 402, 722, 730, 773
BmrFI CCNGG 1 cut(s) 776
BmrI ACTGGG 1 cut(s) 338
BmsI GCATC 1 cut(s) 364
BmuI ACTGGG 1 cut(s) 338
BpiI GAAGAC 1 cut(s) 401
BpuMI CCSGG 1 cut(s) 776
BsaAI YACGTR 1 cut(s) 599
BsaHI GRCGYC 1 cut(s) 57
BsaJI CCNNGG 5 cut(s) 100, 166, 625, 724, 774
Bse118I RCCGGY 1 cut(s) 560
Bse1I ACTGG 2 cut(s) 333, 577
Bse3DI GCAATG 1 cut(s) 150
BseDI CCNNGG 5 cut(s) 100, 166, 625, 724, 774
BseGI GGATG 3 cut(s) 68, 202, 970
BseMI GCAATG 1 cut(s) 150
BseMII CTCAG 1 cut(s) 647
BseNI ACTGG 2 cut(s) 333, 577
BseRI GAGGAG 3 cut(s) 459, 816, 849
BseX3I CGGCCG 1 cut(s) 693
BseXI GCAGC 1 cut(s) 750
BsgI GTGCAG 2 cut(s) 869, 881
Bsh1285I CGRYCG 1 cut(s) 696
BshFI GGCC 6 cut(s) 7, 141, 325, 403, 695, 772
BsiEI CGRYCG 1 cut(s) 696
BsiHKCI CYCGRG 1 cut(s) 889
BsiSI CCGG 2 cut(s) 561, 776
BslFI GGGAC 1 cut(s) 434
BsmFI GGGAC 1 cut(s) 434
BsmI GAATGC 1 cut(s) 910
BsnI GGCC 6 cut(s) 7, 141, 325, 403, 695, 772
BsoBI CYCGRG 1 cut(s) 889
Bsp1286I GDGCHC 1 cut(s) 822
Bsp143I GATC 4 cut(s) 358, 913, 1034, 1062
BspACI CCGC 2 cut(s) 419, 660
BspANI GGCC 6 cut(s) 7, 141, 325, 403, 695, 772
BspCNI CTCAG 1 cut(s) 648
BspLI GGNNCC 5 cut(s) 178, 402, 722, 730, 773
BspMAI CTGCAG 1 cut(s) 768
BspMI ACCTGC 1 cut(s) 946
BspPI GGATC 2 cut(s) 908, 1042
BsrBI CCGCTC 1 cut(s) 660
BsrDI GCAATG 1 cut(s) 150
BsrFI RCCGGY 1 cut(s) 560
BsrI ACTGG 2 cut(s) 333, 577
BssAI RCCGGY 1 cut(s) 560
BssECI CCNNGG 5 cut(s) 100, 166, 625, 724, 774
BssMI GATC 4 cut(s) 358, 913, 1034, 1062
BssNI GRCGYC 1 cut(s) 57
BssT1I CCWWGG 1 cut(s) 100
Bst4CI ACNGT 4 cut(s) 43, 113, 629, 827
Bst6I CTCTTC 1 cut(s) 826
BstACI GRCGYC 1 cut(s) 57
BstBAI YACGTR 1 cut(s) 599
BstC8I GCNNGC 3 cut(s) 126, 578, 990
BstDEI CTNAG 1 cut(s) 656
BstDSI CCRYGG 2 cut(s) 166, 625
BstF5I GGATG 3 cut(s) 68, 202, 970
BstKTI GATC 4 cut(s) 361, 916, 1037, 1065
BstMBI GATC 4 cut(s) 358, 913, 1034, 1062
BstMCI CGRYCG 1 cut(s) 696
BstMWI GCNNNNNNNGC 3 cut(s) 56, 147, 769
BstSCI CCNGG 1 cut(s) 774
BstSFI CTRYAG 2 cut(s) 438, 764
BstV1I GCAGC 1 cut(s) 750
BstV2I GAAGAC 1 cut(s) 401
BstZI CGGCCG 1 cut(s) 693
BsuRI GGCC 6 cut(s) 7, 141, 325, 403, 695, 772
BtgI CCRYGG 2 cut(s) 166, 625
BtsCI GGATG 3 cut(s) 68, 202, 970
BtsI GCAGTG 1 cut(s) 526
BtsIMutI CAGTG 4 cut(s) 326, 526, 832, 1044
BveI ACCTGC 1 cut(s) 946
Cac8I GCNNGC 3 cut(s) 126, 578, 990
Cfr10I RCCGGY 1 cut(s) 560
Cfr13I GGNCC 7 cut(s) 140, 176, 221, 401, 553, 728, 771
CseI GACGC 1 cut(s) 65
Csp6I GTAC 3 cut(s) 201, 600, 617
CspCI CAANNNNNGTGG 2 cut(s) 630, 665
CviAII CATG 1 cut(s) 740
CviQI GTAC 3 cut(s) 201, 600, 617
DdeI CTNAG 1 cut(s) 656
DpnI GATC 4 cut(s) 360, 915, 1036, 1064
DpnII GATC 4 cut(s) 358, 913, 1034, 1062
EaeI YGGCCR 2 cut(s) 5, 693
EagI CGGCCG 1 cut(s) 693
Eam1104I CTCTTC 1 cut(s) 826
EarI CTCTTC 1 cut(s) 826
EciI GGCGGA 1 cut(s) 434
EclXI CGGCCG 1 cut(s) 693
Eco130I CCWWGG 1 cut(s) 100
Eco24I GRGCYC 1 cut(s) 822
Eco47I GGWCC 4 cut(s) 176, 221, 553, 728
Eco52I CGGCCG 1 cut(s) 693
Eco88I CYCGRG 1 cut(s) 889
EcoO109I RGGNCCY 5 cut(s) 176, 221, 401, 728, 771
EcoT14I CCWWGG 1 cut(s) 100
EcoT38I GRGCYC 1 cut(s) 822
ErhI CCWWGG 1 cut(s) 100
FaeI CATG 1 cut(s) 743
FaiI YATR 9 cut(s) 47, 280, 316, 440, 541, 718, 741, 869, 1053
FaqI GGGAC 1 cut(s) 434
FatI CATG 1 cut(s) 739
FbaI TGATCA 1 cut(s) 1062
Fnu4HI GCNGC 2 cut(s) 661, 764
FokI GGATG 3 cut(s) 55, 209, 977
FriOI GRGCYC 1 cut(s) 822
Fsp4HI GCNGC 2 cut(s) 661, 764
FspBI CTAG 2 cut(s) 129, 557
GluI GCNGC 2 cut(s) 661, 764
HaeIII GGCC 6 cut(s) 7, 141, 325, 403, 695, 772
HapII CCGG 2 cut(s) 561, 776
HgaI GACGC 1 cut(s) 65
Hin1I GRCGYC 1 cut(s) 57
Hin1II CATG 1 cut(s) 743
HincII GTYRAC 1 cut(s) 97
HindII GTYRAC 1 cut(s) 97
HinfI GANTC 5 cut(s) 205, 447, 495, 689, 705
HpaII CCGG 2 cut(s) 561, 776
HphI GGTGA 4 cut(s) 244, 575, 937, 1054
Hpy166II GTNNAC 2 cut(s) 97, 466
Hpy188I TCNGA 4 cut(s) 196, 336, 657, 725
Hpy188III TCNNGA 3 cut(s) 53, 787, 1066
Hpy8I GTNNAC 2 cut(s) 97, 466
Hpy99I CGWCG 1 cut(s) 689
HpyAV CCTTC 4 cut(s) 21, 259, 463, 528
HpyCH4III ACNGT 4 cut(s) 43, 113, 629, 827
HpyCH4IV ACGT 3 cut(s) 183, 297, 598
HpyCH4V TGCA 7 cut(s) 371, 641, 766, 850, 862, 955, 988
HpyF10VI GCNNNNNNNGC 3 cut(s) 56, 147, 769
HpyF3I CTNAG 1 cut(s) 656
HpySE526I ACGT 3 cut(s) 183, 297, 598
Hsp92I GRCGYC 1 cut(s) 57
Hsp92II CATG 1 cut(s) 743
Ksp22I TGATCA 1 cut(s) 1062
Kzo9I GATC 4 cut(s) 358, 913, 1034, 1062
LmnI GCTCC 4 cut(s) 39, 55, 627, 726
Lsp1109I GCAGC 1 cut(s) 750
LweI GCATC 1 cut(s) 364
MaeI CTAG 2 cut(s) 129, 557
MaeII ACGT 3 cut(s) 183, 297, 598
MaeIII GTNAC 3 cut(s) 298, 563, 1042
MalI GATC 4 cut(s) 360, 915, 1036, 1064
MbiI CCGCTC 1 cut(s) 660
MboI GATC 4 cut(s) 358, 913, 1034, 1062
MboII GAAGA 4 cut(s) 406, 497, 843, 1019
MhlI GDGCHC 1 cut(s) 822
MlsI TGGCCA 1 cut(s) 7
MluCI AATT 1 cut(s) 433
MluNI TGGCCA 1 cut(s) 7
MlyI GAGTC 1 cut(s) 456
MmeI TCCRAC 2 cut(s) 215, 726
Mox20I TGGCCA 1 cut(s) 7
MscI TGGCCA 1 cut(s) 7
MseI TTAA 1 cut(s) 840
Msp20I TGGCCA 1 cut(s) 7
MspA1I CMGCKG 1 cut(s) 763
MspI CCGG 2 cut(s) 561, 776
MspR9I CCNGG 1 cut(s) 776
Mva1269I GAATGC 1 cut(s) 910
MwoI GCNNNNNNNGC 3 cut(s) 56, 147, 769
NciI CCSGG 1 cut(s) 776
NdeII GATC 4 cut(s) 358, 913, 1034, 1062
NlaIII CATG 1 cut(s) 743
NlaIV GGNNCC 5 cut(s) 178, 402, 722, 730, 773
NmeAIII GCCGAG 1 cut(s) 721
NmuCI GTSAC 3 cut(s) 298, 563, 1042
PcsI WCGNNNNNNNCGW 3 cut(s) 200, 303, 693
PctI GAATGC 1 cut(s) 910
PfeI GAWTC 4 cut(s) 205, 495, 689, 705
PflMI CCANNNNNTGG 2 cut(s) 317, 631
PkrI GCNGC 2 cut(s) 662, 765
PleI GAGTC 1 cut(s) 455
PpsI GAGTC 1 cut(s) 455
Ppu21I YACGTR 1 cut(s) 599
PpuMI RGGWCCY 3 cut(s) 176, 221, 728
Psp5II RGGWCCY 3 cut(s) 176, 221, 728
PspN4I GGNNCC 5 cut(s) 178, 402, 722, 730, 773
PspPI GGNCC 7 cut(s) 140, 176, 221, 401, 553, 728, 771
PspPPI RGGWCCY 3 cut(s) 176, 221, 728
PstI CTGCAG 1 cut(s) 768
PvuII CAGCTG 1 cut(s) 763
RsaI GTAC 3 cut(s) 202, 601, 618
RsaNI GTAC 3 cut(s) 201, 600, 617
SaqAI TTAA 1 cut(s) 840
SatI GCNGC 2 cut(s) 661, 764
Sau3AI GATC 4 cut(s) 358, 913, 1034, 1062
Sau96I GGNCC 7 cut(s) 140, 176, 221, 401, 553, 728, 771
ScaI AGTACT 1 cut(s) 618
SchI GAGTC 1 cut(s) 456
ScrFI CCNGG 1 cut(s) 776
SduI GDGCHC 1 cut(s) 822
SfaNI GCATC 1 cut(s) 364
SfcI CTRYAG 2 cut(s) 438, 764
SinI GGWCC 4 cut(s) 176, 221, 553, 728
Sse9I AATT 1 cut(s) 433
SsiI CCGC 2 cut(s) 419, 660
SspMI CTAG 2 cut(s) 129, 557
StyD4I CCNGG 1 cut(s) 774
StyI CCWWGG 1 cut(s) 100
TaaI ACNGT 4 cut(s) 43, 113, 629, 827
TaiI ACGT 3 cut(s) 186, 300, 601
TaqI TCGA 1 cut(s) 687
TaqII GACCGA 1 cut(s) 541
TasI AATT 1 cut(s) 433
TatI WGTACW 1 cut(s) 616
TauI GCSGC 1 cut(s) 663
TfiI GAWTC 4 cut(s) 205, 495, 689, 705
Tru1I TTAA 1 cut(s) 840
Tru9I TTAA 1 cut(s) 840
TscAI CASTG 4 cut(s) 333, 526, 832, 1044
TseFI GTSAC 3 cut(s) 298, 563, 1042
TseI GCWGC 1 cut(s) 763
Tsp45I GTSAC 3 cut(s) 298, 563, 1042
TspDTI ATGAA 4 cut(s) 407, 498, 887, 897
TspGWI ACGGA 2 cut(s) 82, 254
TspRI CASTG 4 cut(s) 333, 526, 832, 1044
Van91I CCANNNNNTGG 2 cut(s) 317, 631
VpaK11BI GGWCC 4 cut(s) 176, 221, 553, 728
XspI CTAG 2 cut(s) 129, 557
ZrmI AGTACT 1 cut(s) 618
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.