Rroxscaffold_4G00329510

strictosidine

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0000674
Physical Location & Seq
Reverse (-)
62833760 .. 62839525
5766 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00329510.1

Sequence Viewer

Length: 942 bp
ATGAAATCAGCTCTTGTTATTCTCTTCTTCTTCTGTTTGTCTGTTCACCAATCCCAAGGAGCATCTTTTTCACTCCCAAACCTAAACCATCATGAAATACCCATCCACGTACCACAGCCCGAAAGCATTGCTTTCGACTGCAATGGCGCTCCCTACGTGGGTGTGGCTGATGGCAGGATATTCAAGTGGCTAGGATACCCTTTTGGGTGGAGAGAATTCGCAGTCACCTCCCCAATAGGCCCAGAGGAGTATACAGGACCAAATGGTGGAGTAGCTCAAATACTTGCGACATCTGCTGGAGGAGTTCCTTTCCGTTTCTTGAATGGTTTGGACATTGATCGCCAGAGTGGAATGGTGTACTTCACAGACACCAGTGCTATTTACCAGAGACGAAATTGGGAATTGTCAGTTAGTACAGGTGATAAAACTGGAAGGTTAATGCGATATGATCCATGGACAAAGAATGTCACTGTGTTGCGTAATGACTTGTCTTTTGCAAATGGGGTGGCACTAAGCAAAGACGGTGATTTTGTGCTAGTAACCCAAACCACCGCAAAGAACATTTTGAGGTACTGGCTTCGAGGGCCTAGAGCCAACACTGTTGATATTTTTTTCCAACTGCAAGGAGCCCCAGACAATATCATCCGAAACATAAATGGAGAATTTTGGGTTGCGGAAAATAGTGCCGGAAAAGGTAAAGCGGTCAGGCTTGACGCTCAAGGAAATTTTCTGGACGTGGTAGATGGACTCAACCCGGTGAGTCATGTTGAGCAGCGCTATAGAAATCTTTGGATAGGATCTGTGGTTAATAACTTTGTTGGGGAAGGAGAATCTACAAGCAGCCTATATATAGAGGCTAAAGACGACATAGAAACAACAACTCTCCAATCAATCAAACAGATTATCAAAGCCTCTCCGGAGCAAACCTACCTCGCAACCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Str_synth

Protein Analysis

313

Amino Acids

34.66

Weight (kDa)

5.77

Isoelectric Point (pI)

36.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Str_synth PF03088 108 - 195 3.7e-30 Strictosidine synthase
SGL PF08450 139 - 248 3.5e-11 SMP-30/Gluconolactonase/LRE-like region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 266
AccI GTMKAC 1 cut(s) 251
AccIII TCCGGA 1 cut(s) 916
AciI CCGC 3 cut(s) 552, 674, 701
AclWI GGATC 2 cut(s) 443, 805
AcsI RAATTY 3 cut(s) 215, 662, 724
AfaI GTAC 4 cut(s) 111, 359, 415, 572
AfeI AGCGCT 1 cut(s) 776
AfiI CCNNNNNNNGG 2 cut(s) 158, 266
AgsI TTSAA 2 cut(s) 184, 322
AjiI CACGTC 1 cut(s) 736
AluBI AGCT 2 cut(s) 11, 275
AluI AGCT 2 cut(s) 11, 275
Alw26I GTCTC 1 cut(s) 382
AlwI GGATC 2 cut(s) 443, 805
Aor13HI TCCGGA 1 cut(s) 916
Aor51HI AGCGCT 1 cut(s) 776
AoxI GGCC 2 cut(s) 238, 584
ApeKI GCWGC 2 cut(s) 772, 840
ApoI RAATTY 3 cut(s) 215, 662, 724
ArsI GACNNNNNNTTYG 4 cut(s) 476, 508, 512, 544
AspLEI GCGC 2 cut(s) 149, 777
AspS9I GGNCC 3 cut(s) 239, 257, 584
AsuC2I CCSGG 1 cut(s) 755
AsuHPI GGTGA 5 cut(s) 38, 217, 431, 536, 769
AvaII GGWCC 1 cut(s) 257
BanII GRGCYC 1 cut(s) 631
BbvI GCAGC 2 cut(s) 784, 852
BccI CCATC 4 cut(s) 96, 110, 164, 737
BcgI CGANNNNNNTGC 4 cut(s) 110, 115, 144, 149
BciVI GTATCC 1 cut(s) 188
BcnI CCSGG 1 cut(s) 755
BcoDI GTCTC 1 cut(s) 382
BfaI CTAG 4 cut(s) 191, 536, 588, 940
BfmI CTRYAG 1 cut(s) 778
BfoI RGCGCY 2 cut(s) 150, 778
BfuI GTATCC 1 cut(s) 188
BisI GCNGC 2 cut(s) 773, 841
BlsI GCNGC 2 cut(s) 774, 842
Bme1390I CCNGG 1 cut(s) 755
Bme18I GGWCC 1 cut(s) 257
BmgBI CACGTC 1 cut(s) 736
BmgT120I GGNCC 3 cut(s) 239, 257, 584
BmiI GGNNCC 1 cut(s) 628
BmrFI CCNGG 1 cut(s) 755
BmsI GCATC 1 cut(s) 71
BpmI CTGGAG 1 cut(s) 318
BpuEI CTTGAG 1 cut(s) 702
BpuMI CCSGG 1 cut(s) 755
BsaAI YACGTR 2 cut(s) 109, 157
BsaJI CCNNGG 2 cut(s) 55, 452
BsaWI WCCGGW 1 cut(s) 916
Bsc4I CCNNNNNNNGG 2 cut(s) 158, 266
Bse1I ACTGG 3 cut(s) 372, 433, 578
Bse3DI GCAATG 2 cut(s) 126, 148
BseAI TCCGGA 1 cut(s) 916
BseDI CCNNGG 2 cut(s) 55, 452
BseGI GGATG 2 cut(s) 102, 642
BseLI CCNNNNNNNGG 2 cut(s) 158, 266
BseMI GCAATG 2 cut(s) 126, 148
BseNI ACTGG 3 cut(s) 372, 433, 578
BseRI GAGGAG 2 cut(s) 260, 315
BseXI GCAGC 2 cut(s) 784, 852
BshFI GGCC 2 cut(s) 240, 586
BsiSI CCGG 3 cut(s) 687, 755, 917
BslI CCNNNNNNNGG 2 cut(s) 158, 266
BsmAI GTCTC 1 cut(s) 382
BsmBI CGTCTC 1 cut(s) 382
BsnI GGCC 2 cut(s) 240, 586
Bsp1286I GDGCHC 1 cut(s) 631
Bsp13I TCCGGA 1 cut(s) 916
Bsp143I GATC 3 cut(s) 337, 448, 797
Bsp19I CCATGG 1 cut(s) 452
BspACI CCGC 3 cut(s) 552, 674, 701
BspANI GGCC 2 cut(s) 240, 586
BspEI TCCGGA 1 cut(s) 916
BspHI TCATGA 1 cut(s) 91
BspLI GGNNCC 1 cut(s) 628
BspPI GGATC 2 cut(s) 443, 805
BsrDI GCAATG 2 cut(s) 126, 148
BsrI ACTGG 3 cut(s) 372, 433, 578
BssECI CCNNGG 2 cut(s) 55, 452
BssMI GATC 3 cut(s) 337, 448, 797
BssNAI GTATAC 1 cut(s) 252
BssT1I CCWWGG 2 cut(s) 55, 452
Bst1107I GTATAC 1 cut(s) 252
Bst4CI ACNGT 3 cut(s) 472, 524, 601
Bst6I CTCTTC 1 cut(s) 29
BstBAI YACGTR 2 cut(s) 109, 157
BstDEI CTNAG 1 cut(s) 512
BstDSI CCRYGG 1 cut(s) 452
BstF5I GGATG 2 cut(s) 102, 642
BstH2I RGCGCY 2 cut(s) 150, 778
BstHHI GCGC 2 cut(s) 149, 777
BstKTI GATC 3 cut(s) 340, 451, 800
BstMAI GTCTC 1 cut(s) 382
BstMBI GATC 3 cut(s) 337, 448, 797
BstMWI GCNNNNNNNGC 2 cut(s) 293, 583
BstSCI CCNGG 1 cut(s) 753
BstSFI CTRYAG 1 cut(s) 778
BstV1I GCAGC 2 cut(s) 784, 852
BstX2I RGATCY 1 cut(s) 797
BstYI RGATCY 1 cut(s) 797
BstZ17I GTATAC 1 cut(s) 252
BsuI GTATCC 1 cut(s) 188
BsuRI GGCC 2 cut(s) 240, 586
BtgI CCRYGG 1 cut(s) 452
BtrI CACGTC 1 cut(s) 736
BtsCI GGATG 2 cut(s) 102, 642
BtsIMutI CAGTG 3 cut(s) 379, 468, 597
CciI TCATGA 1 cut(s) 91
CfoI GCGC 2 cut(s) 149, 777
Cfr13I GGNCC 3 cut(s) 239, 257, 584
CseI GACGC 1 cut(s) 722
Csp6I GTAC 4 cut(s) 110, 358, 414, 571
CspCI CAANNNNNGTGG 2 cut(s) 486, 521
CviAII CATG 3 cut(s) 92, 453, 764
CviQI GTAC 4 cut(s) 110, 358, 414, 571
DdeI CTNAG 1 cut(s) 512
DpnI GATC 3 cut(s) 339, 450, 799
DpnII GATC 3 cut(s) 337, 448, 797
Eam1104I CTCTTC 1 cut(s) 29
EarI CTCTTC 1 cut(s) 29
Eco130I CCWWGG 2 cut(s) 55, 452
Eco24I GRGCYC 1 cut(s) 631
Eco47I GGWCC 1 cut(s) 257
Eco47III AGCGCT 1 cut(s) 776
EcoO109I RGGNCCY 1 cut(s) 584
EcoRI GAATTC 1 cut(s) 215
EcoT14I CCWWGG 2 cut(s) 55, 452
EcoT38I GRGCYC 1 cut(s) 631
ErhI CCWWGG 2 cut(s) 55, 452
Esp3I CGTCTC 1 cut(s) 382
FaeI CATG 3 cut(s) 95, 456, 767
FalI AAGNNNNNCTT 2 cut(s) 115, 147
FatI CATG 3 cut(s) 91, 452, 763
FblI GTMKAC 1 cut(s) 251
Fnu4HI GCNGC 2 cut(s) 773, 841
FokI GGATG 2 cut(s) 89, 629
FriOI GRGCYC 1 cut(s) 631
Fsp4HI GCNGC 2 cut(s) 773, 841
FspBI CTAG 4 cut(s) 191, 536, 588, 940
GlaI GCGC 2 cut(s) 148, 776
GluI GCNGC 2 cut(s) 773, 841
GsuI CTGGAG 1 cut(s) 318
HaeII RGCGCY 2 cut(s) 150, 778
HaeIII GGCC 2 cut(s) 240, 586
HapII CCGG 3 cut(s) 687, 755, 917
HgaI GACGC 1 cut(s) 722
HhaI GCGC 2 cut(s) 149, 777
Hin1II CATG 3 cut(s) 95, 456, 767
Hin6I GCGC 2 cut(s) 147, 775
HinP1I GCGC 2 cut(s) 147, 775
HinfI GANTC 3 cut(s) 747, 760, 830
HpaII CCGG 3 cut(s) 687, 755, 917
HphI GGTGA 5 cut(s) 38, 217, 431, 536, 769
Hpy166II GTNNAC 3 cut(s) 46, 252, 358
Hpy188I TCNGA 1 cut(s) 647
Hpy188III TCNNGA 4 cut(s) 92, 319, 731, 917
Hpy8I GTNNAC 3 cut(s) 46, 252, 358
HpyAV CCTTC 2 cut(s) 426, 818
HpyCH4III ACNGT 3 cut(s) 472, 524, 601
HpyCH4IV ACGT 3 cut(s) 108, 156, 735
HpyCH4V TGCA 3 cut(s) 141, 497, 622
HpyF10VI GCNNNNNNNGC 2 cut(s) 293, 583
HpyF3I CTNAG 1 cut(s) 512
HpySE526I ACGT 3 cut(s) 108, 156, 735
Hsp92II CATG 3 cut(s) 95, 456, 767
HspAI GCGC 2 cut(s) 147, 775
Kpn2I TCCGGA 1 cut(s) 916
Kzo9I GATC 3 cut(s) 337, 448, 797
LmnI GCTCC 4 cut(s) 59, 154, 626, 919
Lsp1109I GCAGC 2 cut(s) 784, 852
LweI GCATC 1 cut(s) 71
MaeI CTAG 4 cut(s) 191, 536, 588, 940
MaeII ACGT 3 cut(s) 108, 156, 735
MaeIII GTNAC 3 cut(s) 223, 466, 538
MalI GATC 3 cut(s) 339, 450, 799
MboI GATC 3 cut(s) 337, 448, 797
MboII GAAGA 3 cut(s) 16, 19, 22
MflI RGATCY 1 cut(s) 797
MhlI GDGCHC 1 cut(s) 631
MluCI AATT 5 cut(s) 215, 394, 401, 662, 724
MlyI GAGTC 2 cut(s) 741, 769
MmeI TCCRAC 1 cut(s) 640
MnlI CCTC 8 cut(s) 238, 238, 293, 561, 575, 847, 922, 941
MroI TCCGGA 1 cut(s) 916
MseI TTAA 2 cut(s) 437, 807
MspI CCGG 3 cut(s) 687, 755, 917
MspR9I CCNGG 1 cut(s) 755
MwoI GCNNNNNNNGC 2 cut(s) 293, 583
NciI CCSGG 1 cut(s) 755
NcoI CCATGG 1 cut(s) 452
NdeII GATC 3 cut(s) 337, 448, 797
NlaIII CATG 3 cut(s) 95, 456, 767
NlaIV GGNNCC 1 cut(s) 628
NmuCI GTSAC 2 cut(s) 223, 466
PagI TCATGA 1 cut(s) 91
PfeI GAWTC 1 cut(s) 830
PflMI CCANNNNNTGG 1 cut(s) 266
PkrI GCNGC 2 cut(s) 774, 842
PleI GAGTC 2 cut(s) 741, 768
PpsI GAGTC 2 cut(s) 741, 768
Ppu21I YACGTR 2 cut(s) 109, 157
PspN4I GGNNCC 1 cut(s) 628
PspPI GGNCC 3 cut(s) 239, 257, 584
PsuI RGATCY 1 cut(s) 797
RsaI GTAC 4 cut(s) 111, 359, 415, 572
RsaNI GTAC 4 cut(s) 110, 358, 414, 571
SaqAI TTAA 2 cut(s) 437, 807
SatI GCNGC 2 cut(s) 773, 841
Sau3AI GATC 3 cut(s) 337, 448, 797
Sau96I GGNCC 3 cut(s) 239, 257, 584
SchI GAGTC 2 cut(s) 741, 769
ScrFI CCNGG 1 cut(s) 755
SduI GDGCHC 1 cut(s) 631
SfaNI GCATC 1 cut(s) 71
SfcI CTRYAG 1 cut(s) 778
SinI GGWCC 1 cut(s) 257
SmlI CTYRAG 1 cut(s) 717
SmoI CTYRAG 1 cut(s) 717
Sse9I AATT 5 cut(s) 215, 394, 401, 662, 724
SsiI CCGC 3 cut(s) 552, 674, 701
SspMI CTAG 4 cut(s) 191, 536, 588, 940
StyD4I CCNGG 1 cut(s) 753
StyI CCWWGG 2 cut(s) 55, 452
TaaI ACNGT 3 cut(s) 472, 524, 601
TaiI ACGT 3 cut(s) 111, 159, 738
TaqI TCGA 2 cut(s) 135, 580
TasI AATT 5 cut(s) 215, 394, 401, 662, 724
TatI WGTACW 2 cut(s) 357, 413
TfiI GAWTC 1 cut(s) 830
Tru1I TTAA 2 cut(s) 437, 807
Tru9I TTAA 2 cut(s) 437, 807
TscAI CASTG 3 cut(s) 379, 475, 604
TseFI GTSAC 2 cut(s) 223, 466
TseI GCWGC 2 cut(s) 772, 840
Tsp45I GTSAC 2 cut(s) 223, 466
TspDTI ATGAA 2 cut(s) 17, 108
TspGWI ACGGA 1 cut(s) 302
TspRI CASTG 3 cut(s) 379, 475, 604
Van91I CCANNNNNTGG 1 cut(s) 266
VpaK11BI GGWCC 1 cut(s) 257
XapI RAATTY 3 cut(s) 215, 662, 724
XmiI GTMKAC 1 cut(s) 251
XspI CTAG 4 cut(s) 191, 536, 588, 940
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.