Rroxscaffold_3G00259990

strictosidine

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0000674
Physical Location & Seq
Reverse (-)
54348849 .. 54384248
35400 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00259990.1

Sequence Viewer

Length: 1431 bp
ATGTCGGAGTCACAAACGGATGAATATTCAAGTGGCTCAAACAACAAGGTTGGATGGAGTTTGCGACGACTTCTTCTCCACACAGAACAAGAGCAGTATATGATGAATCAACAAGCGAAGATTATGATCCAATTTTCCCTCGACAGGCATGCTGTACTTTACAGACACAATAGCACCACTTATGAAATGAGACAATGGAAACTAGCGGTCGTCTCTGGTGACAAAATTGGAAGACTAATGCGACATGATCCACAAACAAATACGGTGACAACGTTGCTTAGTGGCTTGGCTTGTCCAAATGGTGTGGCTATAAGCAAACTCATTTATTTTGTGTTAGTAGCTCAAACAACCGAGAAGAATGTTACACGATATTGGCTTCAAGGGCCAAGAGCTGGAGAAAGAGAAATTTTTGTTTCACTGCAAGGTGCACCAGTCAATATTTGCAGAAACCAAAACGAAGACTTATGGATTGCTGAAAATAGTGCTGGAGAAGGTAAAGCAATTAAGTTTGACGCGCAAGGAAGATTTATGGAAGTAATAGAGGGACTCCGGCCCTTGGGTCATGTTGAAGAACGCCTTGGAAAAATCCGGAACGGGGCAATATATGGTAAAGTCAAGCGACACTCAGTTTTATTCAGTGAGTGCTTTGATTTGCAAAGATATAGAAAATGCAAATGCAATTATGCAAATGCTGGTAGTGGTAGCAGGCCCGGCCCTGCCCAAGGGCGGGCTTGGCGACGACCTAGGGCCCAAGTCACGTTGTTGTTGGAGTTGGTTTTGGTTCTACTTGCCGTCCATCAGATGCTTTCACGAAGACATAGGTGCTGCTCCCAATTGGACTACTTGCATTTAGGTCATGAAGAGTTACTCTCTTCTTGCTTTTGTATAGTTTTCCTTTCGGTTTTGAAAAAGAGATGGGGATGCTTCATTGTGCAAAAAAAAATATACACACGCATATATAAATCTGGTAAGCCACATTTTAATTCTTCGCCTCAGGCCATTAAAGTTTCAAGACCGGCCACTGGCTATAGGTACTCCGACGTGAAGCACCCACATCTTCAACTAGGATTTGCAGATTTGGATGACTTAAGTCACTTAACGATCTTTAACTACTTAAGTGTGCATAGTTGGATGGCTTTAGTCCGCTTTACTGCTTTGGAGTACCGTGTGGTGACATGTGGGAGTACCCGTGTGGCGAAATACTCTCCGTGTGGTGACATGTGGGGGTACCTGTGTGGGCGAAATACTCCCCGTGTGGATTTTAGAGATTGCAAATCCGGCGCTATTCACAATTTGGGGTTTACAGTTTTCTTAATTGTTGAGGAGTACTGTGTGGTGACATGTGGGAGTACCTGTGTGGCGAAATACTCTCCGTGTGGTGACATGTGGGGGTACTGTGTGGCGAAATACTCCTCGTGTGGTAATATGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Str_synth

Protein Analysis

476

Amino Acids

54.08

Weight (kDa)

9.33

Isoelectric Point (pI)

36.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Str_synth PF03088 51 - 128 9e-19 Strictosidine synthase
SGL PF08450 73 - 181 4.1e-07 SMP-30/Gluconolactonase/LRE-like region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 1227
AccB1I GGYRCC 1 cut(s) 1227
AccB7I CCANNNNNTGG 1 cut(s) 392
AccII CGCG 1 cut(s) 515
AccIII TCCGGA 1 cut(s) 588
AciI CCGC 3 cut(s) 206, 727, 1144
AclI AACGTT 1 cut(s) 272
AclWI GGATC 2 cut(s) 121, 242
AcoI YGGCCR 1 cut(s) 1017
AcsI RAATTY 1 cut(s) 405
AfaI GTAC 8 cut(s) 156, 1034, 1163, 1186, 1229, 1328, 1351, 1394
AfiI CCNNNNNNNGG 8 cut(s) 144, 392, 556, 595, 722, 726, 727, 1022
AflII CTTAAG 2 cut(s) 1087, 1114
AflIII ACRYGT 4 cut(s) 1175, 1218, 1340, 1383
AgsI TTSAA 6 cut(s) 30, 380, 569, 907, 1011, 1061
AjiI CACGTC 1 cut(s) 1042
AjuI GAANNNNNNNTTGG 2 cut(s) 561, 593
AluBI AGCT 2 cut(s) 341, 392
AluI AGCT 2 cut(s) 341, 392
Alw21I GWGCWC 1 cut(s) 430
Alw26I GTCTC 2 cut(s) 184, 217
Alw44I GTGCAC 1 cut(s) 426
AlwI GGATC 2 cut(s) 121, 242
Aor13HI TCCGGA 1 cut(s) 588
AoxI GGCC 7 cut(s) 383, 551, 707, 712, 747, 996, 1017
ApaI GGGCCC 1 cut(s) 751
ApaLI GTGCAC 1 cut(s) 426
ApeKI GCWGC 1 cut(s) 825
ApoI RAATTY 1 cut(s) 405
Asp718I GGTACC 1 cut(s) 1227
AspA2I CCTAGG 1 cut(s) 743
AspLEI GCGC 2 cut(s) 517, 1283
AspS9I GGNCC 6 cut(s) 383, 552, 708, 713, 747, 748
AsuC2I CCSGG 1 cut(s) 711
AsuHPI GGTGA 6 cut(s) 230, 277, 1183, 1226, 1348, 1391
AvrII CCTAGG 1 cut(s) 743
AxyI CCTNAGG 1 cut(s) 993
BaeGI GKGCMC 2 cut(s) 430, 751
BaeI ACNNNNGTAYC 2 cut(s) 1219, 1252
BanI GGYRCC 1 cut(s) 1227
BanII GRGCYC 1 cut(s) 751
BauI CACGAG 1 cut(s) 1414
BbsI GAAGAC 3 cut(s) 238, 465, 820
Bbv12I GWGCWC 1 cut(s) 430
BbvI GCAGC 1 cut(s) 812
BccI CCATC 4 cut(s) 48, 804, 909, 1126
BceAI ACGGC 1 cut(s) 776
BcgI CGANNNNNNTGC 2 cut(s) 131, 165
BcnI CCSGG 1 cut(s) 711
BcoDI GTCTC 2 cut(s) 184, 217
BfaI CTAG 3 cut(s) 203, 744, 1064
BfmI CTRYAG 1 cut(s) 1027
BfoI RGCGCY 1 cut(s) 1284
BfrI CTTAAG 2 cut(s) 1087, 1114
BisI GCNGC 1 cut(s) 826
BlnI CCTAGG 1 cut(s) 743
BlsI GCNGC 1 cut(s) 827
BmcAI AGTACT 1 cut(s) 1328
Bme1390I CCNGG 1 cut(s) 711
BmgBI CACGTC 1 cut(s) 1042
BmgT120I GGNCC 6 cut(s) 383, 552, 708, 713, 747, 748
BmiI GGNNCC 2 cut(s) 749, 1229
BmrFI CCNGG 1 cut(s) 711
BmsI GCATC 2 cut(s) 792, 911
BoxI GACNNNNGTC 1 cut(s) 1089
BpiI GAAGAC 3 cut(s) 238, 465, 820
BplI GAGNNNNNCTC 2 cut(s) 854, 886
BpmI CTGGAG 2 cut(s) 414, 507
BpuMI CCSGG 1 cut(s) 711
BsaBI GATNNNNATC 1 cut(s) 125
BsaJI CCNNGG 4 cut(s) 555, 577, 721, 743
BsaWI WCCGGW 1 cut(s) 588
BsaXI ACNNNNNCTCC 6 cut(s) 60, 90, 1174, 1204, 1339, 1369
Bsc4I CCNNNNNNNGG 8 cut(s) 144, 392, 556, 595, 722, 726, 727, 1022
Bse118I RCCGGY 1 cut(s) 1015
Bse1I ACTGG 2 cut(s) 431, 1027
Bse21I CCTNAGG 1 cut(s) 993
Bse8I GATNNNNATC 1 cut(s) 125
BseAI TCCGGA 1 cut(s) 588
BseDI CCNNGG 4 cut(s) 555, 577, 721, 743
BseGI GGATG 5 cut(s) 25, 59, 926, 1087, 1137
BseJI GATNNNNATC 1 cut(s) 125
BseLI CCNNNNNNNGG 8 cut(s) 144, 392, 556, 595, 722, 726, 727, 1022
BseMII CTCAG 2 cut(s) 639, 1007
BseNI ACTGG 2 cut(s) 431, 1027
BseRI GAGGAG 2 cut(s) 1337, 1402
BseSI GKGCMC 2 cut(s) 430, 751
BseXI GCAGC 1 cut(s) 812
Bsh1236I CGCG 1 cut(s) 515
Bsh1285I CGRYCG 1 cut(s) 210
BshFI GGCC 7 cut(s) 385, 553, 709, 714, 749, 998, 1019
BshNI GGYRCC 1 cut(s) 1227
BsiEI CGRYCG 1 cut(s) 210
BsiHKAI GWGCWC 1 cut(s) 430
BsiSI CCGG 5 cut(s) 550, 589, 711, 1016, 1278
BslFI GGGAC 1 cut(s) 558
BslI CCNNNNNNNGG 8 cut(s) 144, 392, 556, 595, 722, 726, 727, 1022
BsmAI GTCTC 2 cut(s) 184, 217
BsmBI CGTCTC 1 cut(s) 217
BsmFI GGGAC 1 cut(s) 558
BsnI GGCC 7 cut(s) 385, 553, 709, 714, 749, 998, 1019
Bsp120I GGGCCC 1 cut(s) 747
Bsp1286I GDGCHC 2 cut(s) 430, 751
Bsp13I TCCGGA 1 cut(s) 588
Bsp143I GATC 3 cut(s) 126, 247, 1101
BspACI CCGC 3 cut(s) 206, 727, 1144
BspANI GGCC 7 cut(s) 385, 553, 709, 714, 749, 998, 1019
BspCNI CTCAG 2 cut(s) 638, 1006
BspEI TCCGGA 1 cut(s) 588
BspFNI CGCG 1 cut(s) 515
BspHI TCATGA 1 cut(s) 856
BspLI GGNNCC 2 cut(s) 749, 1229
BspPI GGATC 2 cut(s) 121, 242
BspT107I GGYRCC 1 cut(s) 1227
BspTI CTTAAG 2 cut(s) 1087, 1114
BsrFI RCCGGY 1 cut(s) 1015
BsrI ACTGG 2 cut(s) 431, 1027
BssAI RCCGGY 1 cut(s) 1015
BssECI CCNNGG 4 cut(s) 555, 577, 721, 743
BssMI GATC 3 cut(s) 126, 247, 1101
BssSI CACGAG 1 cut(s) 1414
BssT1I CCWWGG 4 cut(s) 555, 577, 721, 743
Bst2BI CACGAG 1 cut(s) 1414
Bst4CI ACNGT 5 cut(s) 265, 1166, 1306, 1331, 1397
Bst6I CTCTTC 2 cut(s) 855, 877
BstAFI CTTAAG 2 cut(s) 1087, 1114
BstC8I GCNNGC 3 cut(s) 150, 707, 729
BstDEI CTNAG 3 cut(s) 278, 625, 993
BstENI CCTNNNNNAGG 1 cut(s) 720
BstF5I GGATG 5 cut(s) 25, 59, 926, 1087, 1137
BstFNI CGCG 1 cut(s) 515
BstH2I RGCGCY 1 cut(s) 1284
BstHHI GCGC 2 cut(s) 517, 1283
BstKTI GATC 3 cut(s) 129, 250, 1104
BstMAI GTCTC 2 cut(s) 184, 217
BstMBI GATC 3 cut(s) 126, 247, 1101
BstMCI CGRYCG 1 cut(s) 210
BstMWI GCNNNNNNNGC 4 cut(s) 382, 711, 733, 1278
BstNSI RCATGY 5 cut(s) 152, 1179, 1222, 1344, 1387
BstPAI GACNNNNGTC 1 cut(s) 1089
BstSCI CCNGG 1 cut(s) 709
BstSFI CTRYAG 1 cut(s) 1027
BstSLI GKGCMC 2 cut(s) 430, 751
BstUI CGCG 1 cut(s) 515
BstV1I GCAGC 1 cut(s) 812
BstV2I GAAGAC 3 cut(s) 238, 465, 820
Bsu36I CCTNAGG 1 cut(s) 993
BsuRI GGCC 7 cut(s) 385, 553, 709, 714, 749, 998, 1019
BtrI CACGTC 1 cut(s) 1042
BtsCI GGATG 5 cut(s) 25, 59, 926, 1087, 1137
BtsI GCAGTG 1 cut(s) 416
BtsIMutI CAGTG 3 cut(s) 416, 643, 1020
Cac8I GCNNGC 3 cut(s) 150, 707, 729
CciI TCATGA 1 cut(s) 856
CfoI GCGC 2 cut(s) 517, 1283
Cfr10I RCCGGY 1 cut(s) 1015
Cfr13I GGNCC 6 cut(s) 383, 552, 708, 713, 747, 748
CseI GACGC 1 cut(s) 521
Csp6I GTAC 8 cut(s) 155, 1033, 1162, 1185, 1228, 1327, 1350, 1393
CspCI CAANNNNNGTGG 2 cut(s) 285, 320
CviAII CATG 8 cut(s) 149, 245, 563, 857, 1176, 1219, 1341, 1384
CviQI GTAC 8 cut(s) 155, 1033, 1162, 1185, 1228, 1327, 1350, 1393
DdeI CTNAG 3 cut(s) 278, 625, 993
DpnI GATC 3 cut(s) 128, 249, 1103
DpnII GATC 3 cut(s) 126, 247, 1101
EaeI YGGCCR 1 cut(s) 1017
Eam1104I CTCTTC 2 cut(s) 855, 877
EarI CTCTTC 2 cut(s) 855, 877
Eco130I CCWWGG 4 cut(s) 555, 577, 721, 743
Eco24I GRGCYC 1 cut(s) 751
Eco81I CCTNAGG 1 cut(s) 993
EcoNI CCTNNNNNAGG 1 cut(s) 720
EcoO109I RGGNCCY 1 cut(s) 747
EcoT14I CCWWGG 4 cut(s) 555, 577, 721, 743
EcoT38I GRGCYC 1 cut(s) 751
ErhI CCWWGG 4 cut(s) 555, 577, 721, 743
Esp3I CGTCTC 1 cut(s) 217
FaeI CATG 8 cut(s) 152, 248, 566, 860, 1179, 1222, 1344, 1387
FalI AAGNNNNNCTT 2 cut(s) 561, 593
FaqI GGGAC 1 cut(s) 558
FatI CATG 8 cut(s) 148, 244, 562, 856, 1175, 1218, 1340, 1383
FauI CCCGC 1 cut(s) 720
Fnu4HI GCNGC 1 cut(s) 826
FokI GGATG 5 cut(s) 32, 66, 933, 1094, 1144
FriOI GRGCYC 1 cut(s) 751
Fsp4HI GCNGC 1 cut(s) 826
FspBI CTAG 3 cut(s) 203, 744, 1064
GlaI GCGC 2 cut(s) 516, 1282
GluI GCNGC 1 cut(s) 826
GsuI CTGGAG 2 cut(s) 414, 507
HaeII RGCGCY 1 cut(s) 1284
HaeIII GGCC 7 cut(s) 385, 553, 709, 714, 749, 998, 1019
HapII CCGG 5 cut(s) 550, 589, 711, 1016, 1278
HgaI GACGC 1 cut(s) 521
HhaI GCGC 2 cut(s) 517, 1283
Hin1II CATG 8 cut(s) 152, 248, 566, 860, 1179, 1222, 1344, 1387
Hin6I GCGC 2 cut(s) 515, 1281
HinP1I GCGC 2 cut(s) 515, 1281
HinfI GANTC 3 cut(s) 8, 106, 546
HpaII CCGG 5 cut(s) 550, 589, 711, 1016, 1278
HphI GGTGA 6 cut(s) 230, 277, 1183, 1226, 1348, 1391
Hpy166II GTNNAC 2 cut(s) 428, 1302
Hpy188I TCNGA 3 cut(s) 7, 801, 1039
Hpy188III TCNNGA 4 cut(s) 589, 810, 857, 1011
Hpy8I GTNNAC 2 cut(s) 428, 1302
Hpy99I CGWCG 3 cut(s) 69, 741, 1043
HpyAV CCTTC 1 cut(s) 485
HpyCH4III ACNGT 5 cut(s) 265, 1166, 1306, 1331, 1397
HpyCH4IV ACGT 3 cut(s) 272, 758, 1041
HpyF10VI GCNNNNNNNGC 4 cut(s) 382, 711, 733, 1278
HpyF3I CTNAG 3 cut(s) 278, 625, 993
HpySE526I ACGT 3 cut(s) 272, 758, 1041
Hsp92II CATG 8 cut(s) 152, 248, 566, 860, 1179, 1222, 1344, 1387
HspAI GCGC 2 cut(s) 515, 1281
Kpn2I TCCGGA 1 cut(s) 588
KpnI GGTACC 1 cut(s) 1231
Kzo9I GATC 3 cut(s) 126, 247, 1101
LmnI GCTCC 1 cut(s) 833
Lsp1109I GCAGC 1 cut(s) 812
LweI GCATC 2 cut(s) 792, 911
MaeI CTAG 3 cut(s) 203, 744, 1064
MaeII ACGT 3 cut(s) 272, 758, 1041
MalI GATC 3 cut(s) 128, 249, 1103
MboI GATC 3 cut(s) 126, 247, 1101
MfeI CAATTG 1 cut(s) 833
MhlI GDGCHC 2 cut(s) 430, 751
MluCI AATT 9 cut(s) 131, 225, 405, 501, 679, 833, 982, 1291, 1314
MlyI GAGTC 2 cut(s) 17, 540
MmeI TCCRAC 4 cut(s) 31, 747, 1062, 1109
MnlI CCTC 5 cut(s) 149, 535, 1002, 1315, 1423
MroI TCCGGA 1 cut(s) 588
MseI TTAA 8 cut(s) 504, 981, 1002, 1088, 1097, 1107, 1115, 1313
MspCI CTTAAG 2 cut(s) 1087, 1114
MspI CCGG 5 cut(s) 550, 589, 711, 1016, 1278
MspR9I CCNGG 1 cut(s) 711
MunI CAATTG 1 cut(s) 833
MvnI CGCG 1 cut(s) 515
MwoI GCNNNNNNNGC 4 cut(s) 382, 711, 733, 1278
NciI CCSGG 1 cut(s) 711
NdeII GATC 3 cut(s) 126, 247, 1101
NlaIII CATG 8 cut(s) 152, 248, 566, 860, 1179, 1222, 1344, 1387
NlaIV GGNNCC 2 cut(s) 749, 1229
NmuCI GTSAC 9 cut(s) 9, 218, 265, 754, 1091, 1171, 1214, 1336, 1379
NspI RCATGY 5 cut(s) 152, 1179, 1222, 1344, 1387
PaeI GCATGC 1 cut(s) 152
PagI TCATGA 1 cut(s) 856
PciI ACATGT 4 cut(s) 1175, 1218, 1340, 1383
PcsI WCGNNNNNNNCGW 1 cut(s) 269
PfeI GAWTC 1 cut(s) 106
PflMI CCANNNNNTGG 1 cut(s) 392
PkrI GCNGC 1 cut(s) 827
PleI GAGTC 2 cut(s) 16, 540
PpsI GAGTC 2 cut(s) 16, 540
PscI ACATGT 4 cut(s) 1175, 1218, 1340, 1383
PshAI GACNNNNGTC 1 cut(s) 1089
Psp1406I AACGTT 1 cut(s) 272
PspN4I GGNNCC 2 cut(s) 749, 1229
PspOMI GGGCCC 1 cut(s) 747
PspPI GGNCC 6 cut(s) 383, 552, 708, 713, 747, 748
RsaI GTAC 8 cut(s) 156, 1034, 1163, 1186, 1229, 1328, 1351, 1394
RsaNI GTAC 8 cut(s) 155, 1033, 1162, 1185, 1228, 1327, 1350, 1393
SaqAI TTAA 8 cut(s) 504, 981, 1002, 1088, 1097, 1107, 1115, 1313
SatI GCNGC 1 cut(s) 826
Sau3AI GATC 3 cut(s) 126, 247, 1101
Sau96I GGNCC 6 cut(s) 383, 552, 708, 713, 747, 748
ScaI AGTACT 1 cut(s) 1328
SchI GAGTC 2 cut(s) 17, 540
ScrFI CCNGG 1 cut(s) 711
SduI GDGCHC 2 cut(s) 430, 751
SfaNI GCATC 2 cut(s) 792, 911
SfcI CTRYAG 1 cut(s) 1027
SmlI CTYRAG 2 cut(s) 1087, 1114
SmoI CTYRAG 2 cut(s) 1087, 1114
SphI GCATGC 1 cut(s) 152
Sse9I AATT 9 cut(s) 131, 225, 405, 501, 679, 833, 982, 1291, 1314
SsiI CCGC 3 cut(s) 206, 727, 1144
SspI AATATT 2 cut(s) 26, 439
SspMI CTAG 3 cut(s) 203, 744, 1064
StyD4I CCNGG 1 cut(s) 709
StyI CCWWGG 4 cut(s) 555, 577, 721, 743
TaaI ACNGT 5 cut(s) 265, 1166, 1306, 1331, 1397
TaiI ACGT 3 cut(s) 275, 761, 1044
TaqI TCGA 1 cut(s) 141
TasI AATT 9 cut(s) 131, 225, 405, 501, 679, 833, 982, 1291, 1314
TatI WGTACW 2 cut(s) 154, 1326
TfiI GAWTC 1 cut(s) 106
Tru1I TTAA 8 cut(s) 504, 981, 1002, 1088, 1097, 1107, 1115, 1313
Tru9I TTAA 8 cut(s) 504, 981, 1002, 1088, 1097, 1107, 1115, 1313
TscAI CASTG 3 cut(s) 423, 643, 1027
TseFI GTSAC 9 cut(s) 9, 218, 265, 754, 1091, 1171, 1214, 1336, 1379
TseI GCWGC 1 cut(s) 825
Tsp45I GTSAC 9 cut(s) 9, 218, 265, 754, 1091, 1171, 1214, 1336, 1379
TspDTI ATGAA 5 cut(s) 36, 119, 198, 873, 916
TspGWI ACGGA 3 cut(s) 32, 1197, 1362
TspRI CASTG 3 cut(s) 423, 643, 1027
Van91I CCANNNNNTGG 1 cut(s) 392
Vha464I CTTAAG 2 cut(s) 1087, 1114
VneI GTGCAC 1 cut(s) 426
XagI CCTNNNNNAGG 1 cut(s) 720
XapI RAATTY 1 cut(s) 405
XceI RCATGY 5 cut(s) 152, 1179, 1222, 1344, 1387
XmaJI CCTAGG 1 cut(s) 743
XspI CTAG 3 cut(s) 203, 744, 1064
ZrmI AGTACT 1 cut(s) 1328
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.