Rorug04G0295500

strictosidine

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0000674
Physical Location & Seq
Reverse (-)
46968186 .. 46968925
740 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0295500.1

Sequence Viewer

Length: 528 bp
ATGGATTTCTGGCGAAGCCGTACCAGGTGGATTCCTACTCCAGAACAAATAAGAATCCTCAAGGATCTTTACTACGTCAAGGGATTTAAGTGCCCAACTACAGAGCACATTCACGAGATCTGCCTCCAGCTGAACCAGTATGGACATGTTGAGGGTAAGAACATTTACTTTTGGTTCCAAAACGTCAGGGCTCGAGAGAAGCAGATGAATAGGTGTAATCAGGCTGCTCCAGTGCCCATGGGAACTAGTTCTCTTGGTACTGGTGGATCCATTGATCTCAATTTTGGGTCCACTGGTTCTACTGGTGCTGGAGGACAAACATCTCTGCAACAACGAGGAGGAGATCACCAGGAGGTTCAAACTCTTCCTCTGTTCCCCGTGCACGGCGAGGACGTCTTTGGTAACCTGAAGGCTACTTCCGAGGAAGGTAGCGCTTTTGGTTACTATTCTGGTGGCTCAGGCGGTTACCACAGTGGCTCAAACGTTTCTCTTGAGCTCAGCCTCAACCCATCCGGAGCTGCTGACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Str_synth

Protein Analysis

175

Amino Acids

19.13

Weight (kDa)

6.29

Isoelectric Point (pI)

41.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Homeodomain PF00046 7 - 68 1.2e-09 Homeodomain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 396
AccIII TCCGGA 1 cut(s) 512
AciI CCGC 1 cut(s) 462
AclI AACGTT 1 cut(s) 483
AclWI GGATC 3 cut(s) 72, 261, 274
AcuI CTGAAG 1 cut(s) 428
AcyI GRCGYC 1 cut(s) 393
AfaI GTAC 2 cut(s) 22, 259
AfeI AGCGCT 1 cut(s) 433
AfiI CCNNNNNNNGG 1 cut(s) 383
AflIII ACRYGT 1 cut(s) 145
AgsI TTSAA 1 cut(s) 359
AhlI ACTAGT 1 cut(s) 245
AjnI CCWGG 2 cut(s) 23, 348
AluBI AGCT 3 cut(s) 130, 496, 518
AluI AGCT 3 cut(s) 130, 496, 518
Alw21I GWGCWC 3 cut(s) 108, 384, 498
Alw44I GTGCAC 1 cut(s) 380
AlwI GGATC 3 cut(s) 72, 261, 274
Ama87I CYCGRG 1 cut(s) 192
Aor13HI TCCGGA 1 cut(s) 512
Aor51HI AGCGCT 1 cut(s) 433
ApaLI GTGCAC 1 cut(s) 380
ApeKI GCWGC 2 cut(s) 224, 518
Asp700I GAANNNNTTC 1 cut(s) 247
AspLEI GCGC 1 cut(s) 434
AspS9I GGNCC 1 cut(s) 288
AsuHPI GGTGA 1 cut(s) 338
AvaI CYCGRG 1 cut(s) 192
AvaII GGWCC 1 cut(s) 288
BaeGI GKGCMC 3 cut(s) 95, 237, 384
BamHI GGATCC 1 cut(s) 266
BanII GRGCYC 2 cut(s) 193, 498
BauI CACGAG 1 cut(s) 113
Bbv12I GWGCWC 3 cut(s) 108, 384, 498
BbvI GCAGC 2 cut(s) 211, 505
BccI CCATC 1 cut(s) 517
BceAI ACGGC 2 cut(s) 3, 400
BciT130I CCWGG 2 cut(s) 25, 350
BcuI ACTAGT 1 cut(s) 245
BfaI CTAG 2 cut(s) 246, 526
BfmI CTRYAG 1 cut(s) 99
BfoI RGCGCY 1 cut(s) 435
BglII AGATCT 1 cut(s) 117
BisI GCNGC 2 cut(s) 225, 519
BlpI GCTNAGC 1 cut(s) 497
BlsI GCNGC 2 cut(s) 226, 520
Bme1390I CCNGG 2 cut(s) 25, 350
Bme18I GGWCC 1 cut(s) 288
BmeT110I CYCGRG 1 cut(s) 192
BmgT120I GGNCC 1 cut(s) 288
BmiI GGNNCC 3 cut(s) 176, 268, 289
BmrFI CCNGG 2 cut(s) 25, 350
BpmI CTGGAG 4 cut(s) 24, 110, 213, 330
Bpu10I CCTNAGC 1 cut(s) 457
Bpu1102I GCTNAGC 1 cut(s) 497
BpuEI CTTGAG 2 cut(s) 44, 512
BsaHI GRCGYC 1 cut(s) 393
BsaJI CCNNGG 2 cut(s) 237, 420
BsaWI WCCGGW 1 cut(s) 512
Bsc4I CCNNNNNNNGG 1 cut(s) 383
Bse1I ACTGG 5 cut(s) 136, 230, 265, 298, 307
BseAI TCCGGA 1 cut(s) 512
BseBI CCWGG 2 cut(s) 25, 350
BseDI CCNNGG 2 cut(s) 237, 420
BseGI GGATG 1 cut(s) 509
BseLI CCNNNNNNNGG 1 cut(s) 383
BseMII CTCAG 2 cut(s) 471, 511
BseNI ACTGG 5 cut(s) 136, 230, 265, 298, 307
BseRI GAGGAG 2 cut(s) 351, 354
BseSI GKGCMC 3 cut(s) 95, 237, 384
BseXI GCAGC 2 cut(s) 211, 505
BsiHKAI GWGCWC 3 cut(s) 108, 384, 498
BsiHKCI CYCGRG 1 cut(s) 192
BsiSI CCGG 1 cut(s) 513
BslI CCNNNNNNNGG 1 cut(s) 383
BsoBI CYCGRG 1 cut(s) 192
Bsp1286I GDGCHC 6 cut(s) 95, 108, 193, 237, 384, 498
Bsp13I TCCGGA 1 cut(s) 512
Bsp143I GATC 5 cut(s) 64, 117, 266, 274, 343
Bsp1720I GCTNAGC 1 cut(s) 497
Bsp19I CCATGG 1 cut(s) 237
BspACI CCGC 1 cut(s) 462
BspCNI CTCAG 2 cut(s) 470, 510
BspEI TCCGGA 1 cut(s) 512
BspLI GGNNCC 3 cut(s) 176, 268, 289
BspPI GGATC 3 cut(s) 72, 261, 274
BsrI ACTGG 5 cut(s) 136, 230, 265, 298, 307
BssECI CCNNGG 2 cut(s) 237, 420
BssMI GATC 5 cut(s) 64, 117, 266, 274, 343
BssNI GRCGYC 1 cut(s) 393
BssSI CACGAG 1 cut(s) 113
BssT1I CCWWGG 1 cut(s) 237
Bst2BI CACGAG 1 cut(s) 113
Bst2UI CCWGG 2 cut(s) 25, 350
Bst4CI ACNGT 1 cut(s) 473
Bst6I CTCTTC 1 cut(s) 369
BstACI GRCGYC 1 cut(s) 393
BstDEI CTNAG 2 cut(s) 457, 497
BstDSI CCRYGG 1 cut(s) 237
BstEII GGTNACC 2 cut(s) 401, 464
BstF5I GGATG 1 cut(s) 509
BstH2I RGCGCY 1 cut(s) 435
BstHHI GCGC 1 cut(s) 434
BstKTI GATC 5 cut(s) 67, 120, 269, 277, 346
BstMBI GATC 5 cut(s) 64, 117, 266, 274, 343
BstNI CCWGG 2 cut(s) 25, 350
BstNSI RCATGY 1 cut(s) 149
BstPI GGTNACC 2 cut(s) 401, 464
BstSCI CCNGG 2 cut(s) 23, 348
BstSFI CTRYAG 1 cut(s) 99
BstSLI GKGCMC 3 cut(s) 95, 237, 384
BstV1I GCAGC 2 cut(s) 211, 505
BstX2I RGATCY 3 cut(s) 64, 117, 266
BstYI RGATCY 3 cut(s) 64, 117, 266
BtgI CCRYGG 1 cut(s) 237
BtsCI GGATG 1 cut(s) 509
BtsIMutI CAGTG 3 cut(s) 237, 291, 478
CfoI GCGC 1 cut(s) 434
Cfr13I GGNCC 1 cut(s) 288
CsiI ACCWGGT 1 cut(s) 23
Csp6I GTAC 2 cut(s) 21, 258
CviAII CATG 2 cut(s) 146, 238
CviQI GTAC 2 cut(s) 21, 258
DdeI CTNAG 2 cut(s) 457, 497
DpnI GATC 5 cut(s) 66, 119, 268, 276, 345
DpnII GATC 5 cut(s) 64, 117, 266, 274, 343
Eam1104I CTCTTC 1 cut(s) 369
EarI CTCTTC 1 cut(s) 369
Ecl136II GAGCTC 1 cut(s) 496
Eco130I CCWWGG 1 cut(s) 237
Eco24I GRGCYC 2 cut(s) 193, 498
Eco47I GGWCC 1 cut(s) 288
Eco47III AGCGCT 1 cut(s) 433
Eco53kI GAGCTC 1 cut(s) 496
Eco57I CTGAAG 1 cut(s) 428
Eco88I CYCGRG 1 cut(s) 192
Eco91I GGTNACC 2 cut(s) 401, 464
EcoICRI GAGCTC 1 cut(s) 496
EcoO65I GGTNACC 2 cut(s) 401, 464
EcoRII CCWGG 2 cut(s) 23, 348
EcoT14I CCWWGG 1 cut(s) 237
EcoT38I GRGCYC 2 cut(s) 193, 498
ErhI CCWWGG 1 cut(s) 237
FaeI CATG 2 cut(s) 149, 241
FaiI YATR 3 cut(s) 141, 147, 239
FatI CATG 2 cut(s) 145, 237
Fnu4HI GCNGC 2 cut(s) 225, 519
FokI GGATG 1 cut(s) 496
FriOI GRGCYC 2 cut(s) 193, 498
Fsp4HI GCNGC 2 cut(s) 225, 519
FspBI CTAG 2 cut(s) 246, 526
GlaI GCGC 1 cut(s) 433
GluI GCNGC 2 cut(s) 225, 519
GsuI CTGGAG 4 cut(s) 24, 110, 213, 330
HaeII RGCGCY 1 cut(s) 435
HapII CCGG 1 cut(s) 513
HhaI GCGC 1 cut(s) 434
Hin1I GRCGYC 1 cut(s) 393
Hin1II CATG 2 cut(s) 149, 241
Hin6I GCGC 1 cut(s) 432
HinP1I GCGC 1 cut(s) 432
HinfI GANTC 2 cut(s) 31, 54
HpaII CCGG 1 cut(s) 513
HphI GGTGA 1 cut(s) 338
Hpy166II GTNNAC 2 cut(s) 291, 382
Hpy188I TCNGA 1 cut(s) 421
Hpy188III TCNNGA 5 cut(s) 41, 113, 194, 491, 513
Hpy8I GTNNAC 2 cut(s) 291, 382
HpyAV CCTTC 2 cut(s) 403, 419
HpyCH4III ACNGT 1 cut(s) 473
HpyCH4IV ACGT 4 cut(s) 75, 183, 393, 483
HpyCH4V TGCA 2 cut(s) 328, 382
HpyF3I CTNAG 2 cut(s) 457, 497
HpySE526I ACGT 4 cut(s) 75, 183, 393, 483
Hsp92I GRCGYC 1 cut(s) 393
Hsp92II CATG 2 cut(s) 149, 241
HspAI GCGC 1 cut(s) 432
Kpn2I TCCGGA 1 cut(s) 512
Kzo9I GATC 5 cut(s) 64, 117, 266, 274, 343
LmnI GCTCC 2 cut(s) 232, 515
Lsp1109I GCAGC 2 cut(s) 211, 505
MabI ACCWGGT 1 cut(s) 23
MaeI CTAG 2 cut(s) 246, 526
MaeII ACGT 4 cut(s) 75, 183, 393, 483
MaeIII GTNAC 3 cut(s) 401, 440, 464
MalI GATC 5 cut(s) 66, 119, 268, 276, 345
MboI GATC 5 cut(s) 64, 117, 266, 274, 343
MboII GAAGA 1 cut(s) 356
MflI RGATCY 3 cut(s) 64, 117, 266
MhlI GDGCHC 6 cut(s) 95, 108, 193, 237, 384, 498
MluCI AATT 1 cut(s) 280
MroI TCCGGA 1 cut(s) 512
MroXI GAANNNNTTC 1 cut(s) 247
MseI TTAA 1 cut(s) 87
MspA1I CMGCKG 1 cut(s) 130
MspI CCGG 1 cut(s) 513
MspR9I CCNGG 2 cut(s) 25, 350
MvaI CCWGG 2 cut(s) 25, 350
NcoI CCATGG 1 cut(s) 237
NdeII GATC 5 cut(s) 64, 117, 266, 274, 343
NlaIII CATG 2 cut(s) 149, 241
NlaIV GGNNCC 3 cut(s) 176, 268, 289
NspI RCATGY 1 cut(s) 149
PaeR7I CTCGAG 1 cut(s) 192
PciI ACATGT 1 cut(s) 145
PcsI WCGNNNNNNNCGW 1 cut(s) 390
PdmI GAANNNNTTC 1 cut(s) 247
PfeI GAWTC 2 cut(s) 31, 54
PkrI GCNGC 2 cut(s) 226, 520
PscI ACATGT 1 cut(s) 145
Psp124BI GAGCTC 1 cut(s) 498
Psp1406I AACGTT 1 cut(s) 483
Psp6I CCWGG 2 cut(s) 23, 348
PspEI GGTNACC 2 cut(s) 401, 464
PspGI CCWGG 2 cut(s) 23, 348
PspN4I GGNNCC 3 cut(s) 176, 268, 289
PspPI GGNCC 1 cut(s) 288
PsuI RGATCY 3 cut(s) 64, 117, 266
PvuII CAGCTG 1 cut(s) 130
RsaI GTAC 2 cut(s) 22, 259
RsaNI GTAC 2 cut(s) 21, 258
SacI GAGCTC 1 cut(s) 498
SaqAI TTAA 1 cut(s) 87
SatI GCNGC 2 cut(s) 225, 519
Sau3AI GATC 5 cut(s) 64, 117, 266, 274, 343
Sau96I GGNCC 1 cut(s) 288
ScrFI CCNGG 2 cut(s) 25, 350
SduI GDGCHC 6 cut(s) 95, 108, 193, 237, 384, 498
SexAI ACCWGGT 1 cut(s) 23
SfcI CTRYAG 1 cut(s) 99
Sfr274I CTCGAG 1 cut(s) 192
SinI GGWCC 1 cut(s) 288
SlaI CTCGAG 1 cut(s) 192
SmlI CTYRAG 3 cut(s) 59, 192, 491
SmoI CTYRAG 3 cut(s) 59, 192, 491
SpeI ACTAGT 1 cut(s) 245
Sse9I AATT 1 cut(s) 280
SsiI CCGC 1 cut(s) 462
SspMI CTAG 2 cut(s) 246, 526
SstI GAGCTC 1 cut(s) 498
StyD4I CCNGG 2 cut(s) 23, 348
StyI CCWWGG 1 cut(s) 237
TaaI ACNGT 1 cut(s) 473
TaiI ACGT 4 cut(s) 78, 186, 396, 486
TaqI TCGA 1 cut(s) 193
TasI AATT 1 cut(s) 280
TfiI GAWTC 2 cut(s) 31, 54
Tru1I TTAA 1 cut(s) 87
Tru9I TTAA 1 cut(s) 87
TscAI CASTG 3 cut(s) 237, 298, 478
TseI GCWGC 2 cut(s) 224, 518
TspDTI ATGAA 1 cut(s) 221
TspRI CASTG 3 cut(s) 237, 298, 478
VneI GTGCAC 1 cut(s) 380
VpaK11BI GGWCC 1 cut(s) 288
XceI RCATGY 1 cut(s) 149
XhoI CTCGAG 1 cut(s) 192
XmnI GAANNNNTTC 1 cut(s) 247
XspI CTAG 2 cut(s) 246, 526
ZraI GACGTC 1 cut(s) 394
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.