RLG00000030550

strictosidine

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0000674
Physical Location & Seq
Reverse (-)
63388797 .. 63389765
969 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000030550

Sequence Viewer

Length: 969 bp
ATGAAATCAGCTCTTGTTACACTCTTCTTCTTCTTCTTCCTCTTTTTGTCTGTTCACCAAGCCCAAGGAGCATCTTTTTCACTCCCAAACCTAAACCATCATGAAATACCCATCCATGTACCACAGCCCGAAAGCATTGCTTTTGACTGCAATGGCGCACCCTACGTGGGTGTGGCTGATGGCAGGATATTCAAGTGGCTAGGATACCCTTTTGGGTGGAGAGAATTCGCAGTCACCGCCCCCAATAGGCCGAGAGGAGTATGTGATTTAACAACCAACAAAGACAATGAACCGATATGCGGGAGGCCACTTGGTTTAAAATTTGACCTCACAACATGTGAACTGTATATCGCAGATGCCTATTTTGGACTTCTAAAGACAGGACCAAATGGTGGAGTAGCTCAAATACTTGCGACATCTGCTGAAGGAGTTCCTTTCCGTTTCTTAAACGGTGTCGACATTGATCGCCAAAGTGGAATGGTGTACTTCACAGACACCAGTGCTATTTACCAGAGAAGAAATTGGGAATTGTCAGTTAGTACAGGTGATAAAACTGGCAGGTTAATGCGTTATGATCCATGGACAAAGAATGTGACTGTGTTGCGTGATGACTTGTCTTTTGCAAATGGGGTGGCACTAAGCAAAGACCGTGATTTTGTGCTAGTAACCCAAACCACCGCAAAGAACATTTTGAGGTACTGGCTTCGAGGGCCTAGAGCCAACACTGTTGATATTTTTTTCCAACTGCAAGGAGCCCCAGACAATATCATCCGAAACATAAATGGAGAATTTTGGGTTGCTGAAAATAGTGCCGGAAAAGGTAAAGCGGTCAAGCTTGACGCTCAAGGAAATTTTCTGGACGTGGTGGATGGACTCAACCCTGTGAGTCATGTTGAGCAGCGTTATAGAAACCTTTGGATAGGATCTGTGGTTAATAACTTTGTTGGGGTTATTCTTAATTTTGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Str_synth

Protein Analysis

323

Amino Acids

35.94

Weight (kDa)

7.68

Isoelectric Point (pI)

21.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Str_synth PF03088 150 - 237 4.7e-30 Strictosidine synthase
SGL PF08450 181 - 290 2.1e-10 SMP-30/Gluconolactonase/LRE-like region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 549
AccB7I CCANNNNNTGG 1 cut(s) 392
AccI GTMKAC 1 cut(s) 456
AciI CCGC 4 cut(s) 237, 300, 678, 827
AclWI GGATC 2 cut(s) 569, 931
AcsI RAATTY 4 cut(s) 224, 320, 788, 850
AcuI CTGAAG 1 cut(s) 444
AfaI GTAC 4 cut(s) 120, 485, 541, 698
AfiI CCNNNNNNNGG 4 cut(s) 167, 246, 299, 392
AflIII ACRYGT 1 cut(s) 335
AgsI TTSAA 1 cut(s) 193
AjiI CACGTC 1 cut(s) 862
AluBI AGCT 3 cut(s) 11, 401, 835
AluI AGCT 3 cut(s) 11, 401, 835
AlwI GGATC 2 cut(s) 569, 931
AoxI GGCC 3 cut(s) 248, 305, 710
ApeKI GCWGC 1 cut(s) 898
ApoI RAATTY 4 cut(s) 224, 320, 788, 850
ArsI GACNNNNNNTTYG 4 cut(s) 602, 634, 638, 670
Asp700I GAANNNNTTC 1 cut(s) 429
AspLEI GCGC 1 cut(s) 158
AspS9I GGNCC 2 cut(s) 383, 710
AsuHPI GGTGA 3 cut(s) 47, 226, 557
AvaII GGWCC 1 cut(s) 383
BanII GRGCYC 1 cut(s) 757
BbvI GCAGC 1 cut(s) 910
BccI CCATC 4 cut(s) 105, 119, 173, 863
BcgI CGANNNNNNTGC 2 cut(s) 119, 153
BciVI GTATCC 1 cut(s) 197
BfaI CTAG 3 cut(s) 200, 662, 714
BfuAI ACCTGC 1 cut(s) 549
BfuI GTATCC 1 cut(s) 197
BisI GCNGC 1 cut(s) 899
BlsI GCNGC 1 cut(s) 900
Bme18I GGWCC 1 cut(s) 383
BmgBI CACGTC 1 cut(s) 862
BmgT120I GGNCC 2 cut(s) 383, 710
BmiI GGNNCC 1 cut(s) 754
BmsI GCATC 2 cut(s) 80, 346
BpuEI CTTGAG 1 cut(s) 828
BsaAI YACGTR 1 cut(s) 166
BsaJI CCNNGG 2 cut(s) 64, 578
Bsc4I CCNNNNNNNGG 4 cut(s) 167, 246, 299, 392
Bse1I ACTGG 3 cut(s) 498, 559, 704
Bse3DI GCAATG 2 cut(s) 135, 157
BseDI CCNNGG 2 cut(s) 64, 578
BseGI GGATG 3 cut(s) 111, 768, 874
BseLI CCNNNNNNNGG 4 cut(s) 167, 246, 299, 392
BseMI GCAATG 2 cut(s) 135, 157
BseNI ACTGG 3 cut(s) 498, 559, 704
BseRI GAGGAG 1 cut(s) 270
BseXI GCAGC 1 cut(s) 910
BshFI GGCC 3 cut(s) 250, 307, 712
BsiSI CCGG 1 cut(s) 813
BslI CCNNNNNNNGG 4 cut(s) 167, 246, 299, 392
BsnI GGCC 3 cut(s) 250, 307, 712
Bsp1286I GDGCHC 1 cut(s) 757
Bsp143I GATC 3 cut(s) 463, 574, 923
Bsp19I CCATGG 1 cut(s) 578
BspACI CCGC 4 cut(s) 237, 300, 678, 827
BspANI GGCC 3 cut(s) 250, 307, 712
BspHI TCATGA 1 cut(s) 100
BspLI GGNNCC 1 cut(s) 754
BspMI ACCTGC 1 cut(s) 549
BspPI GGATC 2 cut(s) 569, 931
BsrDI GCAATG 2 cut(s) 135, 157
BsrI ACTGG 3 cut(s) 498, 559, 704
BssECI CCNNGG 2 cut(s) 64, 578
BssMI GATC 3 cut(s) 463, 574, 923
BssT1I CCWWGG 2 cut(s) 64, 578
Bst4CI ACNGT 5 cut(s) 345, 452, 598, 650, 727
Bst6I CTCTTC 1 cut(s) 29
BstBAI YACGTR 1 cut(s) 166
BstDEI CTNAG 1 cut(s) 638
BstDSI CCRYGG 1 cut(s) 578
BstF5I GGATG 3 cut(s) 111, 768, 874
BstHHI GCGC 1 cut(s) 158
BstKTI GATC 3 cut(s) 466, 577, 926
BstMBI GATC 3 cut(s) 463, 574, 923
BstMWI GCNNNNNNNGC 4 cut(s) 68, 236, 419, 709
BstNSI RCATGY 1 cut(s) 339
BstV1I GCAGC 1 cut(s) 910
BstX2I RGATCY 1 cut(s) 923
BstYI RGATCY 1 cut(s) 923
BsuI GTATCC 1 cut(s) 197
BsuRI GGCC 3 cut(s) 250, 307, 712
BtgI CCRYGG 1 cut(s) 578
BtrI CACGTC 1 cut(s) 862
BtsCI GGATG 3 cut(s) 111, 768, 874
BtsIMutI CAGTG 2 cut(s) 505, 723
BveI ACCTGC 1 cut(s) 549
CciI TCATGA 1 cut(s) 100
CfoI GCGC 1 cut(s) 158
Cfr13I GGNCC 2 cut(s) 383, 710
CseI GACGC 1 cut(s) 848
Csp6I GTAC 4 cut(s) 119, 484, 540, 697
CspCI CAANNNNNGTGG 2 cut(s) 612, 647
CviAII CATG 5 cut(s) 101, 116, 336, 579, 890
CviQI GTAC 4 cut(s) 119, 484, 540, 697
DdeI CTNAG 1 cut(s) 638
DpnI GATC 3 cut(s) 465, 576, 925
DpnII GATC 3 cut(s) 463, 574, 923
DraI TTTAAA 1 cut(s) 318
Eam1104I CTCTTC 1 cut(s) 29
EarI CTCTTC 1 cut(s) 29
Eco130I CCWWGG 2 cut(s) 64, 578
Eco24I GRGCYC 1 cut(s) 757
Eco47I GGWCC 1 cut(s) 383
Eco57I CTGAAG 1 cut(s) 444
EcoO109I RGGNCCY 1 cut(s) 710
EcoRI GAATTC 1 cut(s) 224
EcoT14I CCWWGG 2 cut(s) 64, 578
EcoT38I GRGCYC 1 cut(s) 757
ErhI CCWWGG 2 cut(s) 64, 578
FaeI CATG 5 cut(s) 104, 119, 339, 582, 893
FalI AAGNNNNNCTT 2 cut(s) 124, 156
FatI CATG 5 cut(s) 100, 115, 335, 578, 889
FauI CCCGC 1 cut(s) 293
FblI GTMKAC 1 cut(s) 456
Fnu4HI GCNGC 1 cut(s) 899
FokI GGATG 3 cut(s) 98, 755, 881
FriOI GRGCYC 1 cut(s) 757
Fsp4HI GCNGC 1 cut(s) 899
FspBI CTAG 3 cut(s) 200, 662, 714
GlaI GCGC 1 cut(s) 157
GluI GCNGC 1 cut(s) 899
HaeIII GGCC 3 cut(s) 250, 307, 712
HapII CCGG 1 cut(s) 813
HgaI GACGC 1 cut(s) 848
HhaI GCGC 1 cut(s) 158
Hin1II CATG 5 cut(s) 104, 119, 339, 582, 893
Hin6I GCGC 1 cut(s) 156
HinP1I GCGC 1 cut(s) 156
HincII GTYRAC 1 cut(s) 457
HindII GTYRAC 1 cut(s) 457
HindIII AAGCTT 1 cut(s) 833
HinfI GANTC 2 cut(s) 873, 886
HpaII CCGG 1 cut(s) 813
HphI GGTGA 3 cut(s) 47, 226, 557
Hpy166II GTNNAC 4 cut(s) 55, 341, 457, 484
Hpy188I TCNGA 1 cut(s) 773
Hpy188III TCNNGA 2 cut(s) 101, 857
Hpy8I GTNNAC 4 cut(s) 55, 341, 457, 484
HpyAV CCTTC 1 cut(s) 419
HpyCH4III ACNGT 5 cut(s) 345, 452, 598, 650, 727
HpyCH4IV ACGT 2 cut(s) 165, 861
HpyCH4V TGCA 3 cut(s) 150, 623, 748
HpyF10VI GCNNNNNNNGC 4 cut(s) 68, 236, 419, 709
HpyF3I CTNAG 1 cut(s) 638
HpySE526I ACGT 2 cut(s) 165, 861
Hsp92II CATG 5 cut(s) 104, 119, 339, 582, 893
HspAI GCGC 1 cut(s) 156
Kzo9I GATC 3 cut(s) 463, 574, 923
LmnI GCTCC 2 cut(s) 68, 752
Lsp1109I GCAGC 1 cut(s) 910
LweI GCATC 2 cut(s) 80, 346
MaeI CTAG 3 cut(s) 200, 662, 714
MaeII ACGT 2 cut(s) 165, 861
MaeIII GTNAC 4 cut(s) 16, 232, 592, 664
MalI GATC 3 cut(s) 465, 576, 925
MboI GATC 3 cut(s) 463, 574, 923
MboII GAAGA 6 cut(s) 16, 19, 22, 25, 28, 528
MflI RGATCY 1 cut(s) 923
MhlI GDGCHC 1 cut(s) 757
MluCI AATT 7 cut(s) 224, 320, 520, 527, 788, 850, 958
MlyI GAGTC 2 cut(s) 867, 895
MmeI TCCRAC 1 cut(s) 766
MnlI CCTC 6 cut(s) 50, 248, 297, 338, 687, 701
MroXI GAANNNNTTC 1 cut(s) 429
MseI TTAA 6 cut(s) 269, 317, 446, 563, 933, 957
MspI CCGG 1 cut(s) 813
MwoI GCNNNNNNNGC 4 cut(s) 68, 236, 419, 709
NcoI CCATGG 1 cut(s) 578
NdeII GATC 3 cut(s) 463, 574, 923
NlaIII CATG 5 cut(s) 104, 119, 339, 582, 893
NlaIV GGNNCC 1 cut(s) 754
NmeAIII GCCGAG 1 cut(s) 276
NmuCI GTSAC 2 cut(s) 232, 592
NspI RCATGY 1 cut(s) 339
PagI TCATGA 1 cut(s) 100
PciI ACATGT 1 cut(s) 335
PdmI GAANNNNTTC 1 cut(s) 429
PflMI CCANNNNNTGG 1 cut(s) 392
PkrI GCNGC 1 cut(s) 900
PleI GAGTC 2 cut(s) 867, 894
PpsI GAGTC 2 cut(s) 867, 894
Ppu21I YACGTR 1 cut(s) 166
PscI ACATGT 1 cut(s) 335
PspN4I GGNNCC 1 cut(s) 754
PspPI GGNCC 2 cut(s) 383, 710
PsuI RGATCY 1 cut(s) 923
RsaI GTAC 4 cut(s) 120, 485, 541, 698
RsaNI GTAC 4 cut(s) 119, 484, 540, 697
SalI GTCGAC 1 cut(s) 455
SaqAI TTAA 6 cut(s) 269, 317, 446, 563, 933, 957
SatI GCNGC 1 cut(s) 899
Sau3AI GATC 3 cut(s) 463, 574, 923
Sau96I GGNCC 2 cut(s) 383, 710
SchI GAGTC 2 cut(s) 867, 895
SduI GDGCHC 1 cut(s) 757
SfaNI GCATC 2 cut(s) 80, 346
SinI GGWCC 1 cut(s) 383
SmlI CTYRAG 1 cut(s) 843
SmoI CTYRAG 1 cut(s) 843
Sse9I AATT 7 cut(s) 224, 320, 520, 527, 788, 850, 958
SsiI CCGC 4 cut(s) 237, 300, 678, 827
SspMI CTAG 3 cut(s) 200, 662, 714
StyI CCWWGG 2 cut(s) 64, 578
TaaI ACNGT 5 cut(s) 345, 452, 598, 650, 727
TaiI ACGT 2 cut(s) 168, 864
TaqI TCGA 2 cut(s) 456, 706
TasI AATT 7 cut(s) 224, 320, 520, 527, 788, 850, 958
TatI WGTACW 2 cut(s) 483, 539
Tru1I TTAA 6 cut(s) 269, 317, 446, 563, 933, 957
Tru9I TTAA 6 cut(s) 269, 317, 446, 563, 933, 957
TscAI CASTG 2 cut(s) 505, 730
TseFI GTSAC 2 cut(s) 232, 592
TseI GCWGC 1 cut(s) 898
Tsp45I GTSAC 2 cut(s) 232, 592
TspDTI ATGAA 3 cut(s) 17, 117, 303
TspGWI ACGGA 1 cut(s) 428
TspRI CASTG 2 cut(s) 505, 730
Van91I CCANNNNNTGG 1 cut(s) 392
VpaK11BI GGWCC 1 cut(s) 383
XapI RAATTY 4 cut(s) 224, 320, 788, 850
XceI RCATGY 1 cut(s) 339
XmiI GTMKAC 1 cut(s) 456
XmnI GAANNNNTTC 1 cut(s) 429
XspI CTAG 3 cut(s) 200, 662, 714
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.