FvH4_6g16730

Bifunctional monodehydroascorbate reductase and carbonic anhydrase nectarin-3-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0001343
Physical Location & Seq
Forward (+)
10628258 .. 10630713
2456 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g16730.t1

Sequence Viewer

Length: 876 bp
ATGCTCGGTCTTCTTATATTTTCGGAAAGGACGTACGTAGTGATGGTTAAGTCAAAAACTCCTCTTCAGTTCGTTGCTTTCACTCTTTTTCTTCTGCACTGGCAACCAATCAAACCCATCAGAGCTGGCCATGGAATGGAATTAGAAATAGGGTATGATTATAGCGAAGGGAGCGGGACAGGTCCAAAGCACTGGGGAGAGCTTAGGAAAGAATGGAAGGCATGCAAAGATGAAAAATTGCAATCTCCAATAAATTTGTTGGATGATCAGGCTAGAAAAGCAGTCTCAAGAGTATATGATCTGAAAATGAACTATAAGCCTTCAAATGCAACAATGATGAATGAAGGTCATGCTATTTCGCTTGAATGGGAGGGTGATGCCGGATCCATTCAGATCAATGGCACCGATTACTTCCTCAAACAATGCCATTGGCATAGATCATCTGAACATTTCATTAATGGCAGACGTTACGTTTTGGAATTGCACATGGTTCACCGAAGCCGGAATAACAGAGTAGCTGTAGTAGCATACCTCTACCAAATTGGTAAACGCAGTCCTGTTCTCGCAAAGATGAACAATATGTACGTGTCATCTCTAAGTACTGGTTTAAAGAAGGAAATAAACTTGGGAGTGGCTAATCCAAGAACGATAATACAGCCAAGCTCACAATTTTACAGATATATGGGCTCACTCACAACGCCTCCTTGCACAGGAGGAGTTATTTGGAGCGTAAACAAAAAGGTACTTTCTATTTCGAAAGGACAGCTGAGGTTTCTTGAACGATATGGAAATGCAAAGATGAATGCTAGACCATTACAACCTCTTAATGGTAGGGATATCAGTCTCCTTAGTTGGCCCTCCGTGGGGGTCGCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00910 map00910
Pfam Domains
Protein Families
Carb_anhydrase

Protein Analysis

292

Amino Acids

33.06

Weight (kDa)

9.78

Isoelectric Point (pI)

42.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Carb_anhydrase PF00194 61 - 281 9e-38 Eukaryotic-type carbonic anhydrase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 401
AccB7I CCANNNNNTGG 1 cut(s) 136
AccBSI CCGCTC 1 cut(s) 174
AciI CCGC 1 cut(s) 174
AclWI GGATC 2 cut(s) 378, 391
AcoI YGGCCR 1 cut(s) 127
AcsI RAATTY 1 cut(s) 253
AcuI CTGAAG 1 cut(s) 50
AfaI GTAC 4 cut(s) 35, 584, 601, 744
AfiI CCNNNNNNNGG 5 cut(s) 136, 710, 827, 863, 864
AflIII ACRYGT 1 cut(s) 585
AgsI TTSAA 3 cut(s) 324, 365, 779
AluBI AGCT 5 cut(s) 125, 202, 518, 663, 766
AluI AGCT 5 cut(s) 125, 202, 518, 663, 766
Alw26I GTCTC 2 cut(s) 289, 848
AlwI GGATC 2 cut(s) 378, 391
AoxI GGCC 2 cut(s) 127, 854
ApoI RAATTY 1 cut(s) 253
AseI ATTAAT 1 cut(s) 456
AspS9I GGNCC 2 cut(s) 182, 855
AsuHPI GGTGA 2 cut(s) 386, 485
AsuII TTCGAA 1 cut(s) 755
AvaII GGWCC 1 cut(s) 182
BalI TGGCCA 1 cut(s) 129
BamHI GGATCC 1 cut(s) 383
BanI GGYRCC 1 cut(s) 401
BanII GRGCYC 1 cut(s) 689
BbsI GAAGAC 1 cut(s) 2
BbvCI CCTCAGC 1 cut(s) 767
BccI CCATC 2 cut(s) 37, 125
BclI TGATCA 1 cut(s) 265
BcoDI GTCTC 2 cut(s) 289, 848
BfaI CTAG 2 cut(s) 273, 807
BfmI CTRYAG 1 cut(s) 519
BmcAI AGTACT 1 cut(s) 601
Bme18I GGWCC 1 cut(s) 182
BmgT120I GGNCC 2 cut(s) 182, 855
BmiI GGNNCC 2 cut(s) 385, 403
BmrI ACTGGG 1 cut(s) 202
BmsI GCATC 1 cut(s) 367
BmuI ACTGGG 1 cut(s) 202
BpiI GAAGAC 1 cut(s) 2
Bpu10I CCTNAGC 2 cut(s) 203, 767
Bpu14I TTCGAA 1 cut(s) 755
BpuEI CTTGAG 1 cut(s) 271
BsaAI YACGTR 2 cut(s) 37, 586
BsaJI CCNNGG 2 cut(s) 130, 861
BsaXI ACNNNNNCTCC 2 cut(s) 685, 715
Bsc4I CCNNNNNNNGG 5 cut(s) 136, 710, 827, 863, 864
Bse1I ACTGG 3 cut(s) 104, 197, 607
BseDI CCNNGG 2 cut(s) 130, 861
BseGI GGATG 1 cut(s) 268
BseLI CCNNNNNNNGG 5 cut(s) 136, 710, 827, 863, 864
BseMII CTCAG 1 cut(s) 758
BseNI ACTGG 3 cut(s) 104, 197, 607
BseRI GAGGAG 2 cut(s) 51, 729
BsgI GTGCAG 1 cut(s) 80
BshFI GGCC 2 cut(s) 129, 856
BshNI GGYRCC 1 cut(s) 401
BsiSI CCGG 2 cut(s) 381, 502
BsiWI CGTACG 1 cut(s) 33
BslFI GGGAC 1 cut(s) 190
BslI CCNNNNNNNGG 5 cut(s) 136, 710, 827, 863, 864
BsmAI GTCTC 2 cut(s) 289, 848
BsmFI GGGAC 1 cut(s) 190
BsmI GAATGC 1 cut(s) 808
BsnI GGCC 2 cut(s) 129, 856
Bsp119I TTCGAA 1 cut(s) 755
Bsp1286I GDGCHC 1 cut(s) 689
Bsp143I GATC 5 cut(s) 265, 298, 383, 393, 437
Bsp19I CCATGG 1 cut(s) 130
BspACI CCGC 1 cut(s) 174
BspANI GGCC 2 cut(s) 129, 856
BspCNI CTCAG 1 cut(s) 759
BspLI GGNNCC 2 cut(s) 385, 403
BspPI GGATC 2 cut(s) 378, 391
BspT104I TTCGAA 1 cut(s) 755
BspT107I GGYRCC 1 cut(s) 401
BsrBI CCGCTC 1 cut(s) 174
BsrI ACTGG 3 cut(s) 104, 197, 607
BssECI CCNNGG 2 cut(s) 130, 861
BssMI GATC 5 cut(s) 265, 298, 383, 393, 437
BssT1I CCWWGG 1 cut(s) 130
Bst6I CTCTTC 1 cut(s) 69
BstBAI YACGTR 2 cut(s) 37, 586
BstBI TTCGAA 1 cut(s) 755
BstC8I GCNNGC 2 cut(s) 127, 223
BstDEI CTNAG 4 cut(s) 203, 596, 767, 848
BstDSI CCRYGG 2 cut(s) 130, 861
BstENI CCTNNNNNAGG 1 cut(s) 708
BstF5I GGATG 1 cut(s) 268
BstKTI GATC 5 cut(s) 268, 301, 386, 396, 440
BstMAI GTCTC 2 cut(s) 289, 848
BstMBI GATC 5 cut(s) 265, 298, 383, 393, 437
BstMWI GCNNNNNNNGC 3 cut(s) 171, 278, 524
BstNSI RCATGY 1 cut(s) 225
BstSFI CTRYAG 1 cut(s) 519
BstSNI TACGTA 1 cut(s) 37
BstV2I GAAGAC 1 cut(s) 2
BstX2I RGATCY 1 cut(s) 383
BstXI CCANNNNNNTGG 1 cut(s) 192
BstYI RGATCY 1 cut(s) 383
BsuRI GGCC 2 cut(s) 129, 856
BtgI CCRYGG 2 cut(s) 130, 861
BtsCI GGATG 1 cut(s) 268
BtsIMutI CAGTG 2 cut(s) 97, 190
Cac8I GCNNGC 2 cut(s) 127, 223
Cfr13I GGNCC 2 cut(s) 182, 855
Csp6I GTAC 4 cut(s) 34, 583, 600, 743
CviAII CATG 4 cut(s) 131, 222, 350, 487
CviQI GTAC 4 cut(s) 34, 583, 600, 743
DdeI CTNAG 4 cut(s) 203, 596, 767, 848
DpnI GATC 5 cut(s) 267, 300, 385, 395, 439
DpnII GATC 5 cut(s) 265, 298, 383, 393, 437
DraI TTTAAA 1 cut(s) 609
EaeI YGGCCR 1 cut(s) 127
Eam1104I CTCTTC 1 cut(s) 69
EarI CTCTTC 1 cut(s) 69
Eco105I TACGTA 1 cut(s) 37
Eco130I CCWWGG 1 cut(s) 130
Eco24I GRGCYC 1 cut(s) 689
Eco32I GATATC 1 cut(s) 838
Eco47I GGWCC 1 cut(s) 182
Eco57I CTGAAG 1 cut(s) 50
EcoNI CCTNNNNNAGG 1 cut(s) 708
EcoRV GATATC 1 cut(s) 838
EcoT14I CCWWGG 1 cut(s) 130
EcoT38I GRGCYC 1 cut(s) 689
ErhI CCWWGG 1 cut(s) 130
FaeI CATG 4 cut(s) 134, 225, 353, 490
FaqI GGGAC 1 cut(s) 190
FatI CATG 4 cut(s) 130, 221, 349, 486
FauI CCCGC 1 cut(s) 167
FbaI TGATCA 1 cut(s) 265
FokI GGATG 1 cut(s) 275
FriOI GRGCYC 1 cut(s) 689
FspBI CTAG 2 cut(s) 273, 807
HaeIII GGCC 2 cut(s) 129, 856
HapII CCGG 2 cut(s) 381, 502
Hin1II CATG 4 cut(s) 134, 225, 353, 490
HpaII CCGG 2 cut(s) 381, 502
HphI GGTGA 2 cut(s) 386, 485
Hpy166II GTNNAC 3 cut(s) 493, 548, 733
Hpy188I TCNGA 5 cut(s) 25, 122, 303, 393, 445
Hpy188III TCNNGA 2 cut(s) 288, 776
Hpy8I GTNNAC 3 cut(s) 493, 548, 733
HpyAV CCTTC 5 cut(s) 161, 211, 330, 338, 607
HpyCH4IV ACGT 5 cut(s) 32, 36, 466, 471, 585
HpyCH4V TGCA 7 cut(s) 97, 225, 241, 329, 484, 708, 794
HpyF10VI GCNNNNNNNGC 3 cut(s) 171, 278, 524
HpyF3I CTNAG 4 cut(s) 203, 596, 767, 848
HpySE526I ACGT 5 cut(s) 32, 36, 466, 471, 585
Hsp92II CATG 4 cut(s) 134, 225, 353, 490
Ksp22I TGATCA 1 cut(s) 265
Kzo9I GATC 5 cut(s) 265, 298, 383, 393, 437
LmnI GCTCC 2 cut(s) 171, 726
LweI GCATC 1 cut(s) 367
MaeI CTAG 2 cut(s) 273, 807
MaeII ACGT 5 cut(s) 32, 36, 466, 471, 585
MaeIII GTNAC 1 cut(s) 467
MalI GATC 5 cut(s) 267, 300, 385, 395, 439
MbiI CCGCTC 1 cut(s) 174
MboI GATC 5 cut(s) 265, 298, 383, 393, 437
MboII GAAGA 2 cut(s) 56, 83
MflI RGATCY 1 cut(s) 383
MhlI GDGCHC 1 cut(s) 689
MlsI TGGCCA 1 cut(s) 129
MluCI AATT 6 cut(s) 140, 236, 253, 479, 540, 668
MluNI TGGCCA 1 cut(s) 129
MmeI TCCRAC 1 cut(s) 240
MnlI CCTC 9 cut(s) 72, 364, 425, 542, 707, 711, 762, 831, 868
Mox20I TGGCCA 1 cut(s) 129
MscI TGGCCA 1 cut(s) 129
MseI TTAA 4 cut(s) 48, 456, 608, 825
Msp20I TGGCCA 1 cut(s) 129
MspA1I CMGCKG 1 cut(s) 766
MspI CCGG 2 cut(s) 381, 502
Mva1269I GAATGC 1 cut(s) 808
MwoI GCNNNNNNNGC 3 cut(s) 171, 278, 524
NcoI CCATGG 1 cut(s) 130
NdeII GATC 5 cut(s) 265, 298, 383, 393, 437
NlaIII CATG 4 cut(s) 134, 225, 353, 490
NlaIV GGNNCC 2 cut(s) 385, 403
NspI RCATGY 1 cut(s) 225
NspV TTCGAA 1 cut(s) 755
PaeI GCATGC 1 cut(s) 225
PcsI WCGNNNNNNNCGW 1 cut(s) 29
PctI GAATGC 1 cut(s) 808
Pfl23II CGTACG 1 cut(s) 33
PflMI CCANNNNNTGG 1 cut(s) 136
Ppu21I YACGTR 2 cut(s) 37, 586
PshBI ATTAAT 1 cut(s) 456
PspLI CGTACG 1 cut(s) 33
PspN4I GGNNCC 2 cut(s) 385, 403
PspPI GGNCC 2 cut(s) 182, 855
PsrI GAACNNNNNNTAC 2 cut(s) 566, 598
PsuI RGATCY 1 cut(s) 383
PvuII CAGCTG 1 cut(s) 766
RsaI GTAC 4 cut(s) 35, 584, 601, 744
RsaNI GTAC 4 cut(s) 34, 583, 600, 743
SaqAI TTAA 4 cut(s) 48, 456, 608, 825
Sau3AI GATC 5 cut(s) 265, 298, 383, 393, 437
Sau96I GGNCC 2 cut(s) 182, 855
ScaI AGTACT 1 cut(s) 601
SduI GDGCHC 1 cut(s) 689
SfaNI GCATC 1 cut(s) 367
SfcI CTRYAG 1 cut(s) 519
SfuI TTCGAA 1 cut(s) 755
SinI GGWCC 1 cut(s) 182
SmlI CTYRAG 1 cut(s) 286
SmoI CTYRAG 1 cut(s) 286
SnaBI TACGTA 1 cut(s) 37
SphI GCATGC 1 cut(s) 225
Sse9I AATT 6 cut(s) 140, 236, 253, 479, 540, 668
SsiI CCGC 1 cut(s) 174
SspMI CTAG 2 cut(s) 273, 807
StyI CCWWGG 1 cut(s) 130
TaiI ACGT 5 cut(s) 35, 39, 469, 474, 588
TaqI TCGA 1 cut(s) 755
TasI AATT 6 cut(s) 140, 236, 253, 479, 540, 668
TatI WGTACW 1 cut(s) 599
Tru1I TTAA 4 cut(s) 48, 456, 608, 825
Tru9I TTAA 4 cut(s) 48, 456, 608, 825
TscAI CASTG 2 cut(s) 104, 197
TspDTI ATGAA 7 cut(s) 246, 323, 353, 357, 442, 587, 815
TspGWI ACGGA 1 cut(s) 850
TspRI CASTG 2 cut(s) 104, 197
Van91I CCANNNNNTGG 1 cut(s) 136
VpaK11BI GGWCC 1 cut(s) 182
VspI ATTAAT 1 cut(s) 456
XagI CCTNNNNNAGG 1 cut(s) 708
XapI RAATTY 1 cut(s) 253
XceI RCATGY 1 cut(s) 225
XspI CTAG 2 cut(s) 273, 807
ZrmI AGTACT 1 cut(s) 601
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.