Rh3AG161600

Bifunctional monodehydroascorbate reductase and carbonic anhydrase nectarin-3-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3A
OG0001343
Physical Location & Seq
Forward (+)
14865916 .. 14870145
4230 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3AG161600.1

Sequence Viewer

Length: 990 bp
ATGTTTGTATCAAAGGAATCCCTTCTTGGCTTCATTGTTTTTACCTTTTTCCTTCTTTGCTGGAACCCGACACCCATCAGAGCAAACGACGATGGGGAAGTAGACTTTGATTATCGGGAAGACCACCCGAGAGGACCACAACACTGGGGATACCTTAAGAAAGAATGGAGCTTATGCAAGGATGGAAAAAGGCAATCACCAATAGAGATATGGGAAAAGAATGCCACCAAAGACATCACAGATTCAAAGGATCTTATAGTGAGTTATAAGCCTGCAAATGCAACTATCAAAAATGAAGAACATGCTATATCGGTTTTATGGGAGGGTGATGCTGGCTCAATTGAAATCAATGGCACAACCTACCTCCTCAAACAATGTCATTGGCACCACCCCTCTGAGCATGTCGTTAATGGCAGAAGGTTTGATCTGGAATTGCACATGGTTCACAAAACTCAGGACATGAGCAAAATAGCTGTAATCGGATTCCTCTACAACTTTGGTGAACCTAATTCTTTTCTCACAAAGGTGACAGGACACATCACTTCTTTGACTGATGTACAAAAGGGGATACCCTTGGGAGTGCTTGATCCAAGGAAGGTGAAAAAGGGTCAACTCCATTACAAGATTGCGAAGCCAGAAAAGGCTGGCTCTAAATTTTACAGATACATGGGCTCTCTCACAACCCCTCCTTGTGCTGAAGAGGTTATTTGGCACATAAACAAAAAGGTACAAACTGCTTCGATAGAACAAGTGAAGCTACTTCAAGATGCAGTTTTTGATGTAAGTTATTGCCTATCTTTGCTTAGTGATTTCCTTGATAAAACTAACTATACAACCACTGTATACATATCAAATTATCAATATATAAGTTCGAACGCATGTAAACTTCTTCTGATAAACTTGGGTGCAGTTTGCGGCGAACAACGCAAGACCATTACAACCTCTCAACGGCCGAGGAATCAAGCTCTATGGGGACTATCATTGACCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00910 map00910
Pfam Domains
Protein Families
Carb_anhydrase

Protein Analysis

329

Amino Acids

37.5

Weight (kDa)

8.33

Isoelectric Point (pI)

36.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Carb_anhydrase PF00194 45 - 259 3.8e-34 Eukaryotic-type carbonic anhydrase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 267
AccB1I GGYRCC 1 cut(s) 384
AccI GTMKAC 2 cut(s) 102, 843
AciI CCGC 1 cut(s) 915
AclWI GGATC 2 cut(s) 258, 581
AcoI YGGCCR 1 cut(s) 950
AcsI RAATTY 1 cut(s) 653
AcuI CTGAAG 1 cut(s) 717
AfaI GTAC 2 cut(s) 558, 729
AfiI CCNNNNNNNGG 1 cut(s) 948
AflII CTTAAG 1 cut(s) 155
AgsI TTSAA 3 cut(s) 246, 344, 764
AjuI GAANNNNNNNTTGG 2 cut(s) 9, 41
AleI CACNNNNGTG 1 cut(s) 524
AluBI AGCT 4 cut(s) 171, 473, 757, 965
AluI AGCT 4 cut(s) 171, 473, 757, 965
AlwI GGATC 2 cut(s) 258, 581
Ama87I CYCGRG 1 cut(s) 127
AoxI GGCC 1 cut(s) 950
ApoI RAATTY 1 cut(s) 653
Asp700I GAANNNNTTC 1 cut(s) 21
AspS9I GGNCC 1 cut(s) 134
AsuHPI GGTGA 5 cut(s) 189, 338, 512, 538, 610
AsuII TTCGAA 1 cut(s) 872
AvaI CYCGRG 1 cut(s) 127
AvaII GGWCC 1 cut(s) 134
BanI GGYRCC 1 cut(s) 384
BanII GRGCYC 1 cut(s) 674
BbsI GAAGAC 1 cut(s) 126
BccI CCATC 3 cut(s) 83, 86, 176
BceAI ACGGC 1 cut(s) 965
BciVI GTATCC 2 cut(s) 143, 561
BfaI CTAG 1 cut(s) 988
BfrI CTTAAG 1 cut(s) 155
BfuI GTATCC 2 cut(s) 143, 561
BisI GCNGC 1 cut(s) 916
BlsI GCNGC 1 cut(s) 917
Bme18I GGWCC 1 cut(s) 134
BmeT110I CYCGRG 1 cut(s) 127
BmgT120I GGNCC 1 cut(s) 134
BmiI GGNNCC 2 cut(s) 65, 386
BmrI ACTGGG 1 cut(s) 154
BmsI GCATC 2 cut(s) 319, 757
BmuI ACTGGG 1 cut(s) 154
BpiI GAAGAC 1 cut(s) 126
Bpu14I TTCGAA 1 cut(s) 872
BsaJI CCNNGG 3 cut(s) 573, 590, 953
BsaXI ACNNNNNCTCC 2 cut(s) 670, 700
Bsc4I CCNNNNNNNGG 1 cut(s) 948
Bse1I ACTGG 1 cut(s) 149
BseDI CCNNGG 3 cut(s) 573, 590, 953
BseGI GGATG 1 cut(s) 187
BseLI CCNNNNNNNGG 1 cut(s) 948
BseMII CTCAG 2 cut(s) 387, 467
BseNI ACTGG 1 cut(s) 149
BseRI GAGGAG 1 cut(s) 356
BseX3I CGGCCG 1 cut(s) 950
BsgI GTGCAG 1 cut(s) 927
Bsh1285I CGRYCG 1 cut(s) 953
BshFI GGCC 1 cut(s) 952
BshNI GGYRCC 1 cut(s) 384
BsiEI CGRYCG 1 cut(s) 953
BsiHKCI CYCGRG 1 cut(s) 127
BslI CCNNNNNNNGG 1 cut(s) 948
BsmI GAATGC 1 cut(s) 226
BsnI GGCC 1 cut(s) 952
BsoBI CYCGRG 1 cut(s) 127
Bsp119I TTCGAA 1 cut(s) 872
Bsp1286I GDGCHC 1 cut(s) 674
Bsp1407I TGTACA 1 cut(s) 556
Bsp143I GATC 3 cut(s) 250, 424, 586
BspACI CCGC 1 cut(s) 915
BspANI GGCC 1 cut(s) 952
BspCNI CTCAG 2 cut(s) 388, 466
BspLI GGNNCC 2 cut(s) 65, 386
BspPI GGATC 2 cut(s) 258, 581
BspT104I TTCGAA 1 cut(s) 872
BspT107I GGYRCC 1 cut(s) 384
BspTI CTTAAG 1 cut(s) 155
BsrGI TGTACA 1 cut(s) 556
BsrI ACTGG 1 cut(s) 149
BssECI CCNNGG 3 cut(s) 573, 590, 953
BssMI GATC 3 cut(s) 250, 424, 586
BssNAI GTATAC 1 cut(s) 844
BssT1I CCWWGG 2 cut(s) 573, 590
Bst1107I GTATAC 1 cut(s) 844
Bst4CI ACNGT 1 cut(s) 841
Bst6I CTCTTC 1 cut(s) 693
BstAFI CTTAAG 1 cut(s) 155
BstAUI TGTACA 1 cut(s) 556
BstBI TTCGAA 1 cut(s) 872
BstC8I GCNNGC 3 cut(s) 273, 334, 646
BstDEI CTNAG 3 cut(s) 396, 453, 803
BstF5I GGATG 1 cut(s) 187
BstKTI GATC 3 cut(s) 253, 427, 589
BstMBI GATC 3 cut(s) 250, 424, 586
BstMCI CGRYCG 1 cut(s) 953
BstMWI GCNNNNNNNGC 1 cut(s) 924
BstNSI RCATGY 3 cut(s) 305, 404, 882
BstV2I GAAGAC 1 cut(s) 126
BstX2I RGATCY 1 cut(s) 250
BstXI CCANNNNNNTGG 1 cut(s) 144
BstYI RGATCY 1 cut(s) 250
BstZ17I GTATAC 1 cut(s) 844
BstZI CGGCCG 1 cut(s) 950
BsuI GTATCC 2 cut(s) 143, 561
BsuRI GGCC 1 cut(s) 952
BtsCI GGATG 1 cut(s) 187
BtsIMutI CAGTG 2 cut(s) 142, 837
Cac8I GCNNGC 3 cut(s) 273, 334, 646
Cfr13I GGNCC 1 cut(s) 134
Csp6I GTAC 2 cut(s) 557, 728
CviAII CATG 6 cut(s) 302, 401, 439, 460, 667, 879
CviQI GTAC 2 cut(s) 557, 728
DdeI CTNAG 3 cut(s) 396, 453, 803
DpnI GATC 3 cut(s) 252, 426, 588
DpnII GATC 3 cut(s) 250, 424, 586
EaeI YGGCCR 1 cut(s) 950
EagI CGGCCG 1 cut(s) 950
Eam1104I CTCTTC 1 cut(s) 693
EarI CTCTTC 1 cut(s) 693
EclXI CGGCCG 1 cut(s) 950
Eco130I CCWWGG 2 cut(s) 573, 590
Eco24I GRGCYC 1 cut(s) 674
Eco47I GGWCC 1 cut(s) 134
Eco52I CGGCCG 1 cut(s) 950
Eco57I CTGAAG 1 cut(s) 717
Eco88I CYCGRG 1 cut(s) 127
EcoT14I CCWWGG 2 cut(s) 573, 590
EcoT38I GRGCYC 1 cut(s) 674
ErhI CCWWGG 2 cut(s) 573, 590
FaeI CATG 6 cut(s) 305, 404, 442, 463, 670, 882
FatI CATG 6 cut(s) 301, 400, 438, 459, 666, 878
FblI GTMKAC 2 cut(s) 102, 843
Fnu4HI GCNGC 1 cut(s) 916
FokI GGATG 1 cut(s) 194
FriOI GRGCYC 1 cut(s) 674
Fsp4HI GCNGC 1 cut(s) 916
FspBI CTAG 1 cut(s) 988
GluI GCNGC 1 cut(s) 916
HaeIII GGCC 1 cut(s) 952
Hin1II CATG 6 cut(s) 305, 404, 442, 463, 670, 882
HincII GTYRAC 1 cut(s) 611
HindII GTYRAC 1 cut(s) 611
HinfI GANTC 4 cut(s) 17, 242, 483, 958
HphI GGTGA 5 cut(s) 189, 338, 512, 538, 610
Hpy166II GTNNAC 6 cut(s) 103, 445, 503, 611, 844, 884
Hpy188I TCNGA 4 cut(s) 80, 397, 482, 894
Hpy188III TCNNGA 4 cut(s) 116, 428, 455, 764
Hpy8I GTNNAC 6 cut(s) 103, 445, 503, 611, 844, 884
Hpy99I CGWCG 1 cut(s) 92
HpyAV CCTTC 4 cut(s) 32, 62, 411, 589
HpyCH4III ACNGT 1 cut(s) 841
HpyCH4V TGCA 6 cut(s) 177, 275, 281, 436, 770, 908
HpyF10VI GCNNNNNNNGC 1 cut(s) 924
HpyF3I CTNAG 3 cut(s) 396, 453, 803
Hsp92II CATG 6 cut(s) 305, 404, 442, 463, 670, 882
Kzo9I GATC 3 cut(s) 250, 424, 586
LmnI GCTCC 1 cut(s) 168
LpnPI CCDG 9 cut(s) 46, 130, 285, 318, 413, 440, 516, 630, 648
LweI GCATC 2 cut(s) 319, 757
MaeI CTAG 1 cut(s) 988
MaeIII GTNAC 1 cut(s) 526
MalI GATC 3 cut(s) 252, 426, 588
MboI GATC 3 cut(s) 250, 424, 586
MboII GAAGA 4 cut(s) 131, 308, 710, 881
MfeI CAATTG 1 cut(s) 339
MflI RGATCY 1 cut(s) 250
MhlI GDGCHC 1 cut(s) 674
MluCI AATT 5 cut(s) 339, 431, 508, 653, 853
MroXI GAANNNNTTC 1 cut(s) 21
MseI TTAA 2 cut(s) 156, 408
MslI CAYNNNNRTG 1 cut(s) 524
MspCI CTTAAG 1 cut(s) 155
MunI CAATTG 1 cut(s) 339
Mva1269I GAATGC 1 cut(s) 226
MwoI GCNNNNNNNGC 1 cut(s) 924
NdeII GATC 3 cut(s) 250, 424, 586
NlaIII CATG 6 cut(s) 305, 404, 442, 463, 670, 882
NlaIV GGNNCC 2 cut(s) 65, 386
NmeAIII GCCGAG 1 cut(s) 978
NmuCI GTSAC 1 cut(s) 526
NspI RCATGY 3 cut(s) 305, 404, 882
NspV TTCGAA 1 cut(s) 872
OliI CACNNNNGTG 1 cut(s) 524
PctI GAATGC 1 cut(s) 226
PdmI GAANNNNTTC 1 cut(s) 21
PfeI GAWTC 4 cut(s) 17, 242, 483, 958
PkrI GCNGC 1 cut(s) 917
PsiI TTATAA 1 cut(s) 267
PspN4I GGNNCC 2 cut(s) 65, 386
PspPI GGNCC 1 cut(s) 134
PsuI RGATCY 1 cut(s) 250
RsaI GTAC 2 cut(s) 558, 729
RsaNI GTAC 2 cut(s) 557, 728
RseI CAYNNNNRTG 1 cut(s) 524
SaqAI TTAA 2 cut(s) 156, 408
SatI GCNGC 1 cut(s) 916
Sau3AI GATC 3 cut(s) 250, 424, 586
Sau96I GGNCC 1 cut(s) 134
SduI GDGCHC 1 cut(s) 674
SfaNI GCATC 2 cut(s) 319, 757
SfuI TTCGAA 1 cut(s) 872
SinI GGWCC 1 cut(s) 134
SmiMI CAYNNNNRTG 1 cut(s) 524
SmlI CTYRAG 1 cut(s) 155
SmoI CTYRAG 1 cut(s) 155
Sse9I AATT 5 cut(s) 339, 431, 508, 653, 853
SsiI CCGC 1 cut(s) 915
SspMI CTAG 1 cut(s) 988
StyI CCWWGG 2 cut(s) 573, 590
TaaI ACNGT 1 cut(s) 841
TaqI TCGA 2 cut(s) 740, 872
TasI AATT 5 cut(s) 339, 431, 508, 653, 853
TatI WGTACW 1 cut(s) 556
TauI GCSGC 1 cut(s) 918
TfiI GAWTC 4 cut(s) 17, 242, 483, 958
Tru1I TTAA 2 cut(s) 156, 408
Tru9I TTAA 2 cut(s) 156, 408
TscAI CASTG 2 cut(s) 149, 844
TseFI GTSAC 1 cut(s) 526
Tsp45I GTSAC 1 cut(s) 526
TspDTI ATGAA 2 cut(s) 22, 309
TspRI CASTG 2 cut(s) 149, 844
Vha464I CTTAAG 1 cut(s) 155
VpaK11BI GGWCC 1 cut(s) 134
XapI RAATTY 1 cut(s) 653
XceI RCATGY 3 cut(s) 305, 404, 882
XcmI CCANNNNNNNNNTGG 1 cut(s) 207
XmiI GTMKAC 2 cut(s) 102, 843
XmnI GAANNNNTTC 1 cut(s) 21
XspI CTAG 1 cut(s) 988
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.