Rh3BG188000

Bifunctional monodehydroascorbate reductase and carbonic anhydrase nectarin-3-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3B
OG0001343
Physical Location & Seq
Reverse (-)
16587522 .. 16589029
1508 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3BG188000.1

Sequence Viewer

Length: 882 bp
ATGGTTAAATCAAAGAAAGTCCATCTTCAGTTCTTTGCTTTCACTCTTTTTCTTCTGTCCTGGCACCCCATCAGACCCGTCAGTGGCCAAGGAGTGGAAGAAATAGGGTATGATTATAGGCAAGGGAGCCCCAAGGGACCAGAGCACTGGGGAGAGCTCAAGGAAGAATGGAAGGAATGCAAAGATGGAAAATTACAATCGCCAATAGATTTGTTGGATTCGAATGCTAGAAAAGCAATCTCAAGAGTAGATGATCTTAACATGAGCTATAAGCCTTCAAATGCAACCATGAAGCATGAAGGTTATGCTATTTCGATTCAATGGGAGGGTGATGCTGGATCAATTCAAATAAATGGCACTGATTACTTGCTCAAAAGATGTCATTGGCACAGATCTTCTGAACATTTCATTAATGGCAGAAGGTTTCTCTTTCTCTTTGCTTCTCTGTTTGAAAAATCCAAAATACTTCATATATGTCTAAACAGAGTTCTTTTTATCAGTTATGATTTGGAATTGCACATGGTTCACCAAAGCCAGAACAACGATGTGGCTGTAGTCGCATTCCTATACCAAAGTGGCAATCCCGATCCTTTCCTGTCAAAGATAAGCAACGATGTATCCTCCTTAAGTGGTACAAAGGAGGCACGCTTGGGAGTGATTGATCCAAGTTTAATAAAATGGCCAAGCTCACGATTTTACAGATACATGGGCTCACTCACGACCCCTCCTTGCACTGGAGGAGTTATTTGGAGCGTATACGAAGAGGTACATTCTGTTTCAGAAGAACAGCTGGGGCTAATTGAACAAGTCGATCATGGGATGAATGCAAGACCATTACAGCCTCTTAACAATCGAGATATCACGCTCTTTGGTCCGCAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00910 map00910
Pfam Domains
Protein Families
Carb_anhydrase

Protein Analysis

293

Amino Acids

33.4

Weight (kDa)

6.64

Isoelectric Point (pI)

49.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Carb_anhydrase PF00194 45 - 288 2.9e-30 Eukaryotic-type carbonic anhydrase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 63
AccB7I CCANNNNNTGG 1 cut(s) 94
AccI GTMKAC 1 cut(s) 756
AciI CCGC 1 cut(s) 875
AclWI GGATC 3 cut(s) 346, 581, 656
AcoI YGGCCR 2 cut(s) 85, 680
AcuI CTGAAG 1 cut(s) 11
AfaI GTAC 2 cut(s) 634, 768
AfiI CCNNNNNNNGG 3 cut(s) 83, 94, 734
AflII CTTAAG 1 cut(s) 625
AgsI TTSAA 5 cut(s) 279, 320, 347, 452, 803
AjnI CCWGG 1 cut(s) 59
AluBI AGCT 4 cut(s) 157, 267, 687, 790
AluI AGCT 4 cut(s) 157, 267, 687, 790
Alw21I GWGCWC 2 cut(s) 147, 159
AlwI GGATC 3 cut(s) 346, 581, 656
AoxI GGCC 2 cut(s) 85, 680
AseI ATTAAT 1 cut(s) 411
AspS9I GGNCC 2 cut(s) 137, 872
AsuHPI GGTGA 2 cut(s) 341, 518
AsuII TTCGAA 1 cut(s) 221
AvaII GGWCC 2 cut(s) 137, 872
BalI TGGCCA 2 cut(s) 87, 682
BanI GGYRCC 1 cut(s) 63
BanII GRGCYC 3 cut(s) 131, 159, 713
Bbv12I GWGCWC 2 cut(s) 147, 159
BccI CCATC 3 cut(s) 30, 77, 179
BciT130I CCWGG 1 cut(s) 61
BciVI GTATCC 1 cut(s) 628
BfaI CTAG 1 cut(s) 228
BfmI CTRYAG 1 cut(s) 552
BfrI CTTAAG 1 cut(s) 625
BfuI GTATCC 1 cut(s) 628
BglII AGATCT 1 cut(s) 392
Bme1390I CCNGG 1 cut(s) 61
Bme18I GGWCC 2 cut(s) 137, 872
BmgT120I GGNCC 2 cut(s) 137, 872
BmiI GGNNCC 3 cut(s) 65, 128, 138
BmrFI CCNGG 1 cut(s) 61
BmrI ACTGGG 1 cut(s) 157
BmsI GCATC 1 cut(s) 322
BmuI ACTGGG 1 cut(s) 157
BpmI CTGGAG 1 cut(s) 756
Bpu14I TTCGAA 1 cut(s) 221
BpuEI CTTGAG 2 cut(s) 143, 226
BsaJI CCNNGG 2 cut(s) 88, 132
BsaXI ACNNNNNCTCC 2 cut(s) 709, 739
Bsc4I CCNNNNNNNGG 3 cut(s) 83, 94, 734
Bse1I ACTGG 2 cut(s) 152, 739
BseBI CCWGG 1 cut(s) 61
BseDI CCNNGG 2 cut(s) 88, 132
BseGI GGATG 1 cut(s) 825
BseLI CCNNNNNNNGG 3 cut(s) 83, 94, 734
BseNI ACTGG 2 cut(s) 152, 739
BseRI GAGGAG 1 cut(s) 753
BseYI CCCAGC 1 cut(s) 790
BshFI GGCC 2 cut(s) 87, 682
BshNI GGYRCC 1 cut(s) 63
BsiHKAI GWGCWC 2 cut(s) 147, 159
BslFI GGGAC 1 cut(s) 150
BslI CCNNNNNNNGG 3 cut(s) 83, 94, 734
BsmFI GGGAC 1 cut(s) 150
BsmI GAATGC 4 cut(s) 182, 229, 560, 829
BsnI GGCC 2 cut(s) 87, 682
Bsp119I TTCGAA 1 cut(s) 221
Bsp1286I GDGCHC 4 cut(s) 131, 147, 159, 713
Bsp143I GATC 6 cut(s) 253, 338, 392, 586, 661, 811
BspACI CCGC 1 cut(s) 875
BspANI GGCC 2 cut(s) 87, 682
BspLI GGNNCC 3 cut(s) 65, 128, 138
BspPI GGATC 3 cut(s) 346, 581, 656
BspT104I TTCGAA 1 cut(s) 221
BspT107I GGYRCC 1 cut(s) 63
BspTI CTTAAG 1 cut(s) 625
BsrI ACTGG 2 cut(s) 152, 739
BssECI CCNNGG 2 cut(s) 88, 132
BssMI GATC 6 cut(s) 253, 338, 392, 586, 661, 811
BssNAI GTATAC 1 cut(s) 757
BssT1I CCWWGG 2 cut(s) 88, 132
Bst1107I GTATAC 1 cut(s) 757
Bst2UI CCWGG 1 cut(s) 61
Bst6I CTCTTC 1 cut(s) 756
BstAFI CTTAAG 1 cut(s) 625
BstBI TTCGAA 1 cut(s) 221
BstC8I GCNNGC 1 cut(s) 646
BstF5I GGATG 1 cut(s) 825
BstKTI GATC 6 cut(s) 256, 341, 395, 589, 664, 814
BstMBI GATC 6 cut(s) 253, 338, 392, 586, 661, 811
BstMWI GCNNNNNNNGC 2 cut(s) 233, 557
BstNI CCWGG 1 cut(s) 61
BstSCI CCNGG 1 cut(s) 59
BstSFI CTRYAG 1 cut(s) 552
BstX2I RGATCY 1 cut(s) 392
BstXI CCANNNNNNTGG 1 cut(s) 147
BstYI RGATCY 1 cut(s) 392
BstZ17I GTATAC 1 cut(s) 757
BsuI GTATCC 1 cut(s) 628
BsuRI GGCC 2 cut(s) 87, 682
BtsCI GGATG 1 cut(s) 825
BtsIMutI CAGTG 4 cut(s) 88, 145, 357, 732
Cac8I GCNNGC 1 cut(s) 646
Cfr13I GGNCC 2 cut(s) 137, 872
Csp6I GTAC 2 cut(s) 633, 767
CviAII CATG 6 cut(s) 262, 289, 296, 520, 706, 815
CviQI GTAC 2 cut(s) 633, 767
DpnI GATC 6 cut(s) 255, 340, 394, 588, 663, 813
DpnII GATC 6 cut(s) 253, 338, 392, 586, 661, 811
EaeI YGGCCR 2 cut(s) 85, 680
Eam1104I CTCTTC 1 cut(s) 756
EarI CTCTTC 1 cut(s) 756
Ecl136II GAGCTC 1 cut(s) 157
Eco130I CCWWGG 2 cut(s) 88, 132
Eco24I GRGCYC 3 cut(s) 131, 159, 713
Eco32I GATATC 1 cut(s) 859
Eco47I GGWCC 2 cut(s) 137, 872
Eco53kI GAGCTC 1 cut(s) 157
Eco57I CTGAAG 1 cut(s) 11
EcoICRI GAGCTC 1 cut(s) 157
EcoRII CCWGG 1 cut(s) 59
EcoRV GATATC 1 cut(s) 859
EcoT14I CCWWGG 2 cut(s) 88, 132
EcoT38I GRGCYC 3 cut(s) 131, 159, 713
ErhI CCWWGG 2 cut(s) 88, 132
FaeI CATG 6 cut(s) 265, 292, 299, 523, 709, 818
FalI AAGNNNNNCTT 2 cut(s) 9, 41
FaqI GGGAC 1 cut(s) 150
FatI CATG 6 cut(s) 261, 288, 295, 519, 705, 814
FblI GTMKAC 1 cut(s) 756
FokI GGATG 1 cut(s) 832
FriOI GRGCYC 3 cut(s) 131, 159, 713
FspBI CTAG 1 cut(s) 228
GsaI CCCAGC 1 cut(s) 794
GsuI CTGGAG 1 cut(s) 756
HaeIII GGCC 2 cut(s) 87, 682
Hin1II CATG 6 cut(s) 265, 292, 299, 523, 709, 818
HinfI GANTC 2 cut(s) 218, 316
HphI GGTGA 2 cut(s) 341, 518
Hpy166II GTNNAC 2 cut(s) 526, 757
Hpy188I TCNGA 3 cut(s) 74, 400, 781
Hpy188III TCNNGA 5 cut(s) 243, 584, 690, 718, 854
Hpy8I GTNNAC 2 cut(s) 526, 757
HpyAV CCTTC 4 cut(s) 166, 285, 293, 414
HpyCH4V TGCA 5 cut(s) 180, 284, 517, 732, 827
HpyF10VI GCNNNNNNNGC 2 cut(s) 233, 557
Hsp92II CATG 6 cut(s) 265, 292, 299, 523, 709, 818
Kzo9I GATC 6 cut(s) 253, 338, 392, 586, 661, 811
LmnI GCTCC 2 cut(s) 126, 750
LpnPI CCDG 9 cut(s) 46, 73, 133, 153, 321, 548, 608, 720, 776
LweI GCATC 1 cut(s) 322
MaeI CTAG 1 cut(s) 228
MalI GATC 6 cut(s) 255, 340, 394, 588, 663, 813
MboI GATC 6 cut(s) 253, 338, 392, 586, 661, 811
MboII GAAGA 7 cut(s) 17, 44, 110, 176, 387, 773, 794
MflI RGATCY 1 cut(s) 392
MhlI GDGCHC 4 cut(s) 131, 147, 159, 713
MlsI TGGCCA 2 cut(s) 87, 682
MluCI AATT 4 cut(s) 191, 342, 512, 798
MluNI TGGCCA 2 cut(s) 87, 682
MmeI TCCRAC 1 cut(s) 195
MnlI CCTC 7 cut(s) 319, 631, 634, 731, 735, 757, 852
Mox20I TGGCCA 2 cut(s) 87, 682
MscI TGGCCA 2 cut(s) 87, 682
MseI TTAA 6 cut(s) 6, 258, 411, 626, 671, 846
Msp20I TGGCCA 2 cut(s) 87, 682
MspA1I CMGCKG 1 cut(s) 790
MspCI CTTAAG 1 cut(s) 625
MspR9I CCNGG 1 cut(s) 61
Mva1269I GAATGC 4 cut(s) 182, 229, 560, 829
MvaI CCWGG 1 cut(s) 61
MwoI GCNNNNNNNGC 2 cut(s) 233, 557
NdeII GATC 6 cut(s) 253, 338, 392, 586, 661, 811
NlaIII CATG 6 cut(s) 265, 292, 299, 523, 709, 818
NlaIV GGNNCC 3 cut(s) 65, 128, 138
NspV TTCGAA 1 cut(s) 221
PctI GAATGC 4 cut(s) 182, 229, 560, 829
PfeI GAWTC 2 cut(s) 218, 316
PflMI CCANNNNNTGG 1 cut(s) 94
PshBI ATTAAT 1 cut(s) 411
Psp124BI GAGCTC 1 cut(s) 159
Psp6I CCWGG 1 cut(s) 59
PspFI CCCAGC 1 cut(s) 790
PspGI CCWGG 1 cut(s) 59
PspN4I GGNNCC 3 cut(s) 65, 128, 138
PspPI GGNCC 2 cut(s) 137, 872
PsuI RGATCY 1 cut(s) 392
PvuII CAGCTG 1 cut(s) 790
RsaI GTAC 2 cut(s) 634, 768
RsaNI GTAC 2 cut(s) 633, 767
SacI GAGCTC 1 cut(s) 159
SaqAI TTAA 6 cut(s) 6, 258, 411, 626, 671, 846
Sau3AI GATC 6 cut(s) 253, 338, 392, 586, 661, 811
Sau96I GGNCC 2 cut(s) 137, 872
ScrFI CCNGG 1 cut(s) 61
SduI GDGCHC 4 cut(s) 131, 147, 159, 713
SetI ASST 7 cut(s) 159, 269, 304, 425, 689, 768, 792
SfaNI GCATC 1 cut(s) 322
SfcI CTRYAG 1 cut(s) 552
SfuI TTCGAA 1 cut(s) 221
SinI GGWCC 2 cut(s) 137, 872
SmlI CTYRAG 3 cut(s) 158, 241, 625
SmoI CTYRAG 3 cut(s) 158, 241, 625
Sse9I AATT 4 cut(s) 191, 342, 512, 798
SsiI CCGC 1 cut(s) 875
SspMI CTAG 1 cut(s) 228
SstI GAGCTC 1 cut(s) 159
StyD4I CCNGG 1 cut(s) 59
StyI CCWWGG 2 cut(s) 88, 132
TaqI TCGA 4 cut(s) 221, 314, 810, 853
TasI AATT 4 cut(s) 191, 342, 512, 798
TfiI GAWTC 2 cut(s) 218, 316
Tru1I TTAA 6 cut(s) 6, 258, 411, 626, 671, 846
Tru9I TTAA 6 cut(s) 6, 258, 411, 626, 671, 846
TscAI CASTG 4 cut(s) 88, 152, 364, 739
TspDTI ATGAA 5 cut(s) 305, 312, 397, 458, 836
TspRI CASTG 4 cut(s) 88, 152, 364, 739
Van91I CCANNNNNTGG 1 cut(s) 94
Vha464I CTTAAG 1 cut(s) 625
VpaK11BI GGWCC 2 cut(s) 137, 872
VspI ATTAAT 1 cut(s) 411
XmiI GTMKAC 1 cut(s) 756
XspI CTAG 1 cut(s) 228
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.