Rorug03G0111900

Bifunctional monodehydroascorbate reductase and carbonic anhydrase nectarin-3-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0001343
Physical Location & Seq
Forward (+)
9041572 .. 9042404
833 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0111900.1

Sequence Viewer

Length: 597 bp
ATGGAGTACTATTTCCCAGCTAGCTCAGATGGTTCCAATTTCTCTTCTTCCTCAGTGATCACCAACACCAACAACAACAACATCAACATCAATGGCGGGCGCAACAAAGACAAAATGAAGAAGAGTACTAGTGGTAATGATCAGAAGAGCAAGAACAAAGTGAAGCTGTCGACAGACCCACAAAGCGTGGCGGCGAGGGAGAGGAGGCACCGCATCAGCGAAAGGTTCAAGATCCTGCAGAGCCTGGTCCCCGGTGGAACCAAGCTGGACACTGTGTCCATGCTGGAGGAAGCCATTCAGTACGTGAAGTTCTTGAAGAGTCAGATATGGCTTCACCAGACCATGATCAACTTTGCGGGAGATGATGATAATCAGTACTACGATCACGACCCTACATCTGGAAATGTGTTCCAGCTTCCGGTTCATCACGGCCATCCAGTTGATCCTCCTCATCAGCAGGCTTTGTGCTATGGGCAGAATAGTTTCGTGGGTACTAGTTCGGTGCAGCCATCGGTTCCAGTAATGCCGGAATATTCTTGCTTCCAAGGTCAAGAAGCTACGCCTAACATTGAAGCCTATATGAAGTATTGTAGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00910 map00910
Pfam Domains
Protein Families
Carb_anhydrase

Protein Analysis

198

Amino Acids

22.19

Weight (kDa)

7.05

Isoelectric Point (pI)

54.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HLH PF00010 65 - 106 8e-10 Helix-loop-helix DNA-binding domain
bHLH_SAC51 PF23173 67 - 108 8.4e-10 Transcription factor SAC51 bHLH domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 207
AccI GTMKAC 1 cut(s) 170
AciI CCGC 4 cut(s) 96, 191, 211, 356
AclWI GGATC 2 cut(s) 226, 437
AcoI YGGCCR 1 cut(s) 430
AfaI GTAC 5 cut(s) 8, 127, 302, 377, 493
AfiI CCNNNNNNNGG 2 cut(s) 398, 418
AgsI TTSAA 3 cut(s) 229, 316, 572
AhdI GACNNNNNGTC 1 cut(s) 274
AhlI ACTAGT 2 cut(s) 128, 494
AjnI CCWGG 1 cut(s) 243
AluBI AGCT 7 cut(s) 20, 24, 166, 265, 415, 557, 594
AluI AGCT 7 cut(s) 20, 24, 166, 265, 415, 557, 594
AlwI GGATC 2 cut(s) 226, 437
AlwNI CAGNNNCTG 1 cut(s) 244
AoxI GGCC 1 cut(s) 430
ApeKI GCWGC 1 cut(s) 505
Asp700I GAANNNNTTC 2 cut(s) 294, 482
AspLEI GCGC 1 cut(s) 102
AspS9I GGNCC 1 cut(s) 247
AsuC2I CCSGG 1 cut(s) 252
AsuHPI GGTGA 2 cut(s) 52, 326
AsuNHI GCTAGC 1 cut(s) 20
AvaII GGWCC 1 cut(s) 247
BanI GGYRCC 1 cut(s) 207
BbvI GCAGC 1 cut(s) 517
BccI CCATC 3 cut(s) 23, 441, 517
BceAI ACGGC 1 cut(s) 445
BciT130I CCWGG 1 cut(s) 245
BclI TGATCA 3 cut(s) 57, 139, 345
BcnI CCSGG 1 cut(s) 252
BcuI ACTAGT 2 cut(s) 128, 494
BfaI CTAG 4 cut(s) 21, 129, 495, 595
BfmI CTRYAG 1 cut(s) 236
BisI GCNGC 2 cut(s) 192, 506
BlsI GCNGC 2 cut(s) 193, 507
BmcAI AGTACT 3 cut(s) 8, 127, 377
Bme1390I CCNGG 2 cut(s) 245, 252
Bme18I GGWCC 1 cut(s) 247
BmeRI GACNNNNNGTC 1 cut(s) 274
BmgT120I GGNCC 1 cut(s) 247
BmiI GGNNCC 5 cut(s) 34, 209, 249, 259, 516
BmrFI CCNGG 2 cut(s) 245, 252
BmsI GCATC 1 cut(s) 222
BmtI GCTAGC 1 cut(s) 24
BpmI CTGGAG 1 cut(s) 305
BpuMI CCSGG 1 cut(s) 252
BsaAI YACGTR 1 cut(s) 304
BsaBI GATNNNNATC 1 cut(s) 369
BsaJI CCNNGG 2 cut(s) 250, 544
BsaWI WCCGGW 1 cut(s) 418
Bsc4I CCNNNNNNNGG 2 cut(s) 398, 418
Bse1I ACTGG 2 cut(s) 437, 518
Bse8I GATNNNNATC 1 cut(s) 369
BseBI CCWGG 1 cut(s) 245
BseDI CCNNGG 2 cut(s) 250, 544
BseGI GGATG 1 cut(s) 433
BseJI GATNNNNATC 1 cut(s) 369
BseLI CCNNNNNNNGG 2 cut(s) 398, 418
BseMII CTCAG 2 cut(s) 39, 66
BseNI ACTGG 2 cut(s) 437, 518
BseRI GAGGAG 2 cut(s) 217, 438
BseXI GCAGC 1 cut(s) 517
BseYI CCCAGC 1 cut(s) 16
BsgI GTGCAG 1 cut(s) 524
BshFI GGCC 1 cut(s) 432
BshNI GGYRCC 1 cut(s) 207
BsiSI CCGG 3 cut(s) 252, 419, 527
BslFI GGGAC 1 cut(s) 233
BslI CCNNNNNNNGG 2 cut(s) 398, 418
BsmFI GGGAC 1 cut(s) 233
BsnI GGCC 1 cut(s) 432
Bsp143I GATC 6 cut(s) 57, 139, 231, 345, 382, 442
BspACI CCGC 4 cut(s) 96, 191, 211, 356
BspANI GGCC 1 cut(s) 432
BspCNI CTCAG 2 cut(s) 38, 65
BspLI GGNNCC 5 cut(s) 34, 209, 249, 259, 516
BspMAI CTGCAG 1 cut(s) 240
BspOI GCTAGC 1 cut(s) 24
BspPI GGATC 2 cut(s) 226, 437
BspQI GCTCTTC 1 cut(s) 140
BspT107I GGYRCC 1 cut(s) 207
BsrI ACTGG 2 cut(s) 437, 518
BssECI CCNNGG 2 cut(s) 250, 544
BssMI GATC 6 cut(s) 57, 139, 231, 345, 382, 442
BssT1I CCWWGG 1 cut(s) 544
Bst2UI CCWGG 1 cut(s) 245
Bst4CI ACNGT 1 cut(s) 274
Bst6I CTCTTC 4 cut(s) 49, 116, 140, 311
BstBAI YACGTR 1 cut(s) 304
BstC8I GCNNGC 3 cut(s) 22, 98, 459
BstDEI CTNAG 2 cut(s) 25, 52
BstF5I GGATG 1 cut(s) 433
BstHHI GCGC 1 cut(s) 102
BstKTI GATC 6 cut(s) 60, 142, 234, 348, 385, 445
BstMBI GATC 6 cut(s) 57, 139, 231, 345, 382, 442
BstNI CCWGG 1 cut(s) 245
BstSCI CCNGG 2 cut(s) 243, 250
BstSFI CTRYAG 1 cut(s) 236
BstV1I GCAGC 1 cut(s) 517
BstX2I RGATCY 1 cut(s) 231
BstYI RGATCY 1 cut(s) 231
BsuRI GGCC 1 cut(s) 432
BtsCI GGATG 1 cut(s) 433
BtsIMutI CAGTG 2 cut(s) 60, 270
Cac8I GCNNGC 3 cut(s) 22, 98, 459
CaiI CAGNNNCTG 1 cut(s) 244
CfoI GCGC 1 cut(s) 102
Cfr13I GGNCC 1 cut(s) 247
Csp6I GTAC 5 cut(s) 7, 126, 301, 376, 492
CviAII CATG 2 cut(s) 280, 343
CviQI GTAC 5 cut(s) 7, 126, 301, 376, 492
DdeI CTNAG 2 cut(s) 25, 52
DpnI GATC 6 cut(s) 59, 141, 233, 347, 384, 444
DpnII GATC 6 cut(s) 57, 139, 231, 345, 382, 442
DriI GACNNNNNGTC 1 cut(s) 274
EaeI YGGCCR 1 cut(s) 430
Eam1104I CTCTTC 4 cut(s) 49, 116, 140, 311
Eam1105I GACNNNNNGTC 1 cut(s) 274
EarI CTCTTC 4 cut(s) 49, 116, 140, 311
Eco130I CCWWGG 1 cut(s) 544
Eco47I GGWCC 1 cut(s) 247
EcoRII CCWGG 1 cut(s) 243
EcoT14I CCWWGG 1 cut(s) 544
ErhI CCWWGG 1 cut(s) 544
FaeI CATG 2 cut(s) 283, 346
FaiI YATR 6 cut(s) 281, 328, 344, 471, 579, 581
FaqI GGGAC 1 cut(s) 233
FatI CATG 2 cut(s) 279, 342
FauI CCCGC 2 cut(s) 89, 349
FbaI TGATCA 3 cut(s) 57, 139, 345
FblI GTMKAC 1 cut(s) 170
Fnu4HI GCNGC 2 cut(s) 192, 506
FokI GGATG 1 cut(s) 420
Fsp4HI GCNGC 2 cut(s) 192, 506
FspBI CTAG 4 cut(s) 21, 129, 495, 595
GlaI GCGC 1 cut(s) 101
GluI GCNGC 2 cut(s) 192, 506
GsaI CCCAGC 1 cut(s) 20
GsuI CTGGAG 1 cut(s) 305
HaeIII GGCC 1 cut(s) 432
HapII CCGG 3 cut(s) 252, 419, 527
HhaI GCGC 1 cut(s) 102
Hin1II CATG 2 cut(s) 283, 346
Hin6I GCGC 1 cut(s) 100
HinP1I GCGC 1 cut(s) 100
HincII GTYRAC 1 cut(s) 171
HindII GTYRAC 1 cut(s) 171
HinfI GANTC 1 cut(s) 319
HpaII CCGG 3 cut(s) 252, 419, 527
HphI GGTGA 2 cut(s) 52, 326
Hpy166II GTNNAC 1 cut(s) 171
Hpy188I TCNGA 3 cut(s) 28, 144, 324
Hpy188III TCNNGA 5 cut(s) 229, 313, 386, 399, 551
Hpy8I GTNNAC 1 cut(s) 171
HpyCH4III ACNGT 1 cut(s) 274
HpyCH4IV ACGT 1 cut(s) 303
HpyCH4V TGCA 2 cut(s) 238, 505
HpyF3I CTNAG 2 cut(s) 25, 52
HpySE526I ACGT 1 cut(s) 303
Hsp92II CATG 2 cut(s) 283, 346
HspAI GCGC 1 cut(s) 100
Ksp22I TGATCA 3 cut(s) 57, 139, 345
Kzo9I GATC 6 cut(s) 57, 139, 231, 345, 382, 442
LguI GCTCTTC 1 cut(s) 140
Lsp1109I GCAGC 1 cut(s) 517
LweI GCATC 1 cut(s) 222
MaeI CTAG 4 cut(s) 21, 129, 495, 595
MaeII ACGT 1 cut(s) 303
MalI GATC 6 cut(s) 59, 141, 233, 347, 384, 444
MboI GATC 6 cut(s) 57, 139, 231, 345, 382, 442
MboII GAAGA 6 cut(s) 36, 39, 130, 133, 157, 328
MflI RGATCY 1 cut(s) 231
MluCI AATT 1 cut(s) 37
MlyI GAGTC 1 cut(s) 328
MnlI CCTC 7 cut(s) 61, 189, 195, 198, 280, 456, 459
MroXI GAANNNNTTC 2 cut(s) 294, 482
MspI CCGG 3 cut(s) 252, 419, 527
MspR9I CCNGG 2 cut(s) 245, 252
MvaI CCWGG 1 cut(s) 245
NciI CCSGG 1 cut(s) 252
NdeII GATC 6 cut(s) 57, 139, 231, 345, 382, 442
NheI GCTAGC 1 cut(s) 20
NlaIII CATG 2 cut(s) 283, 346
NlaIV GGNNCC 5 cut(s) 34, 209, 249, 259, 516
PciSI GCTCTTC 1 cut(s) 140
PdmI GAANNNNTTC 2 cut(s) 294, 482
PkrI GCNGC 2 cut(s) 193, 507
PleI GAGTC 1 cut(s) 327
PpsI GAGTC 1 cut(s) 327
Ppu21I YACGTR 1 cut(s) 304
Psp6I CCWGG 1 cut(s) 243
PspFI CCCAGC 1 cut(s) 16
PspGI CCWGG 1 cut(s) 243
PspN4I GGNNCC 5 cut(s) 34, 209, 249, 259, 516
PspPI GGNCC 1 cut(s) 247
PsrI GAACNNNNNNTAC 2 cut(s) 293, 325
PstI CTGCAG 1 cut(s) 240
PstNI CAGNNNCTG 1 cut(s) 244
PsuI RGATCY 1 cut(s) 231
RsaI GTAC 5 cut(s) 8, 127, 302, 377, 493
RsaNI GTAC 5 cut(s) 7, 126, 301, 376, 492
SalI GTCGAC 1 cut(s) 169
SapI GCTCTTC 1 cut(s) 140
SatI GCNGC 2 cut(s) 192, 506
Sau3AI GATC 6 cut(s) 57, 139, 231, 345, 382, 442
Sau96I GGNCC 1 cut(s) 247
ScaI AGTACT 3 cut(s) 8, 127, 377
SchI GAGTC 1 cut(s) 328
ScrFI CCNGG 2 cut(s) 245, 252
SfaNI GCATC 1 cut(s) 222
SfcI CTRYAG 1 cut(s) 236
SinI GGWCC 1 cut(s) 247
SpeI ACTAGT 2 cut(s) 128, 494
Sse9I AATT 1 cut(s) 37
SsiI CCGC 4 cut(s) 96, 191, 211, 356
SspI AATATT 1 cut(s) 533
SspMI CTAG 4 cut(s) 21, 129, 495, 595
StyD4I CCNGG 2 cut(s) 243, 250
StyI CCWWGG 1 cut(s) 544
TaaI ACNGT 1 cut(s) 274
TaiI ACGT 1 cut(s) 306
TaqI TCGA 1 cut(s) 170
TasI AATT 1 cut(s) 37
TatI WGTACW 3 cut(s) 6, 125, 375
TauI GCSGC 1 cut(s) 194
TscAI CASTG 2 cut(s) 60, 277
TseI GCWGC 1 cut(s) 505
TspDTI ATGAA 3 cut(s) 131, 413, 596
TspRI CASTG 2 cut(s) 60, 277
VpaK11BI GGWCC 1 cut(s) 247
XmiI GTMKAC 1 cut(s) 170
XmnI GAANNNNTTC 2 cut(s) 294, 482
XspI CTAG 4 cut(s) 21, 129, 495, 595
ZrmI AGTACT 3 cut(s) 8, 127, 377
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.