Rw3G015240

Bifunctional monodehydroascorbate reductase and carbonic anhydrase nectarin-3-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr3
OG0001343
Physical Location & Seq
Forward (+)
15659041 .. 15673890
14850 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw3G015240.1

Sequence Viewer

Length: 933 bp
ATGCTGGACCGGCTCATAAGTTCTTTTCTTTTATCTTGCTCCTTAAAGTATCCATTGCTCTTTGCAGACGTAGTGATGATTAGATCAATGAAAACTCCTCTTCAGTTCTTTGCTTTCACTCTTTTTCTTCTGTACTGGCAACCAATCAAACCCATCAGAGCTGGCCATGGTGTGGAAGAAATAGGGTATAATTATAGGAAAGGGAGCAAGAAGGGACCAGAGCACTGGGGAGACCTTAAGAAAGAATGGAAGGCATGCAAAGATGGAAAATTGCAATCGCCAATAAATTTGTCGGATAAGCACGCTAGAAAAGCAATCTCAAGAGTATATGATCTTAAAATGAGCTATAAGCCTTCAAATGCAACTATGAAGAATGAAGGTCATGCTATTGCGATTGAATGGGAGGGTGATGCAGGATCAATTCAGATCAATGGCACTGATTTTTTCCTCAAACAATGCCATTGGCACAGATCCTCTGAACATTTCATTAATGGCAGAAGTTATGATTTGGAATTGCACATGGTTCACCACAGCCGGAAGAACAACGTAGCTGTAGTCGCATTCCTCTACAAAATTGGTAGACGTAGTCCTTTTTTCTCAAAGGTGACGGGACACATCACTTCTTTAGCTGATGTACAAAAGGGGATACCCTTGGGAGTGCTTGATCCAAGGAAGGTGAAAAAGGGTCAACTCCATTACAAGATTGCGAAGCCAGAAAAGGCTGGCTCTAAATTTTACAGATACATGGGCTCTCTCACAACCCCTCCTTGTGCTGAAGAGGTTATTTGGCACATAAACAAAAAGGTACAAACTGCTTCGATAGAACAAGTGAAGCTACTTCAAGATGCAGTTTTTGATTTTGCGGCGAACAACGCAAGACCATTACAACCTCTCAACGGCCGAGGAATCAAGCTCTATGGGGACTATCATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00910 map00910
Pfam Domains
Protein Families
Carb_anhydrase

Protein Analysis

310

Amino Acids

35.39

Weight (kDa)

9.72

Isoelectric Point (pI)

42.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Carb_anhydrase PF00194 71 - 304 1.8e-39 Eukaryotic-type carbonic anhydrase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 172
AccI GTMKAC 1 cut(s) 580
AciI CCGC 1 cut(s) 863
AclWI GGATC 3 cut(s) 424, 465, 659
AcoI YGGCCR 2 cut(s) 163, 898
AcsI RAATTY 2 cut(s) 286, 731
AcuI CTGAAG 2 cut(s) 86, 795
AfaI GTAC 3 cut(s) 134, 636, 807
AfiI CCNNNNNNNGG 2 cut(s) 172, 896
AflII CTTAAG 1 cut(s) 236
AgsI TTSAA 3 cut(s) 357, 398, 842
AluBI AGCT 6 cut(s) 161, 345, 551, 629, 835, 913
AluI AGCT 6 cut(s) 161, 345, 551, 629, 835, 913
Alw21I GWGCWC 1 cut(s) 225
Alw26I GTCTC 1 cut(s) 225
AlwI GGATC 3 cut(s) 424, 465, 659
AoxI GGCC 2 cut(s) 163, 898
ApoI RAATTY 2 cut(s) 286, 731
AseI ATTAAT 1 cut(s) 489
AspS9I GGNCC 2 cut(s) 7, 215
AsuHPI GGTGA 4 cut(s) 419, 518, 616, 688
AvaII GGWCC 2 cut(s) 7, 215
BalI TGGCCA 1 cut(s) 165
BanII GRGCYC 1 cut(s) 752
Bbv12I GWGCWC 1 cut(s) 225
BccI CCATC 2 cut(s) 161, 257
BceAI ACGGC 1 cut(s) 913
BciVI GTATCC 2 cut(s) 60, 639
BcoDI GTCTC 1 cut(s) 225
BfaI CTAG 1 cut(s) 306
BfmI CTRYAG 1 cut(s) 552
BfrI CTTAAG 1 cut(s) 236
BfuI GTATCC 2 cut(s) 60, 639
BisI GCNGC 1 cut(s) 864
BlsI GCNGC 1 cut(s) 865
Bme18I GGWCC 2 cut(s) 7, 215
BmgT120I GGNCC 2 cut(s) 7, 215
BmiI GGNNCC 1 cut(s) 216
BmrI ACTGGG 1 cut(s) 235
BmsI GCATC 2 cut(s) 400, 835
BmuI ACTGGG 1 cut(s) 235
BpuEI CTTGAG 1 cut(s) 304
BsaI GGTCTC 1 cut(s) 225
BsaJI CCNNGG 4 cut(s) 166, 651, 668, 901
BsaXI ACNNNNNCTCC 2 cut(s) 748, 778
Bsc4I CCNNNNNNNGG 2 cut(s) 172, 896
Bse118I RCCGGY 1 cut(s) 9
Bse1I ACTGG 2 cut(s) 140, 230
Bse3DI GCAATG 1 cut(s) 53
BseDI CCNNGG 4 cut(s) 166, 651, 668, 901
BseLI CCNNNNNNNGG 2 cut(s) 172, 896
BseMI GCAATG 1 cut(s) 53
BseNI ACTGG 2 cut(s) 140, 230
BseRI GAGGAG 1 cut(s) 87
BseX3I CGGCCG 1 cut(s) 898
Bsh1285I CGRYCG 1 cut(s) 901
BshFI GGCC 2 cut(s) 165, 900
BsiEI CGRYCG 1 cut(s) 901
BsiHKAI GWGCWC 1 cut(s) 225
BsiSI CCGG 2 cut(s) 10, 535
BslFI GGGAC 2 cut(s) 228, 624
BslI CCNNNNNNNGG 2 cut(s) 172, 896
BsmAI GTCTC 1 cut(s) 225
BsmFI GGGAC 2 cut(s) 228, 624
BsmI GAATGC 1 cut(s) 560
BsnI GGCC 2 cut(s) 165, 900
Bso31I GGTCTC 1 cut(s) 225
Bsp1286I GDGCHC 2 cut(s) 225, 752
Bsp1407I TGTACA 1 cut(s) 634
Bsp143I GATC 6 cut(s) 83, 331, 416, 426, 470, 664
Bsp19I CCATGG 1 cut(s) 166
BspACI CCGC 1 cut(s) 863
BspANI GGCC 2 cut(s) 165, 900
BspLI GGNNCC 1 cut(s) 216
BspPI GGATC 3 cut(s) 424, 465, 659
BspTI CTTAAG 1 cut(s) 236
BspTNI GGTCTC 1 cut(s) 225
BsrDI GCAATG 1 cut(s) 53
BsrFI RCCGGY 1 cut(s) 9
BsrGI TGTACA 1 cut(s) 634
BsrI ACTGG 2 cut(s) 140, 230
BssAI RCCGGY 1 cut(s) 9
BssECI CCNNGG 4 cut(s) 166, 651, 668, 901
BssMI GATC 6 cut(s) 83, 331, 416, 426, 470, 664
BssT1I CCWWGG 3 cut(s) 166, 651, 668
Bst6I CTCTTC 2 cut(s) 105, 771
BstAFI CTTAAG 1 cut(s) 236
BstAUI TGTACA 1 cut(s) 634
BstC8I GCNNGC 4 cut(s) 163, 256, 303, 724
BstDSI CCRYGG 1 cut(s) 166
BstKTI GATC 6 cut(s) 86, 334, 419, 429, 473, 667
BstMAI GTCTC 1 cut(s) 225
BstMBI GATC 6 cut(s) 83, 331, 416, 426, 470, 664
BstMCI CGRYCG 1 cut(s) 901
BstMWI GCNNNNNNNGC 4 cut(s) 10, 311, 557, 872
BstNSI RCATGY 1 cut(s) 258
BstSFI CTRYAG 1 cut(s) 552
BstX2I RGATCY 1 cut(s) 470
BstXI CCANNNNNNTGG 1 cut(s) 225
BstYI RGATCY 1 cut(s) 470
BstZI CGGCCG 1 cut(s) 898
BsuI GTATCC 2 cut(s) 60, 639
BsuRI GGCC 2 cut(s) 165, 900
BtgI CCRYGG 1 cut(s) 166
BtsIMutI CAGTG 2 cut(s) 223, 435
Cac8I GCNNGC 4 cut(s) 163, 256, 303, 724
Cfr10I RCCGGY 1 cut(s) 9
Cfr13I GGNCC 2 cut(s) 7, 215
Csp6I GTAC 3 cut(s) 133, 635, 806
CviAII CATG 5 cut(s) 167, 255, 383, 520, 745
CviQI GTAC 3 cut(s) 133, 635, 806
DpnI GATC 6 cut(s) 85, 333, 418, 428, 472, 666
DpnII GATC 6 cut(s) 83, 331, 416, 426, 470, 664
EaeI YGGCCR 2 cut(s) 163, 898
EagI CGGCCG 1 cut(s) 898
Eam1104I CTCTTC 2 cut(s) 105, 771
EarI CTCTTC 2 cut(s) 105, 771
EclXI CGGCCG 1 cut(s) 898
Eco130I CCWWGG 3 cut(s) 166, 651, 668
Eco24I GRGCYC 1 cut(s) 752
Eco31I GGTCTC 1 cut(s) 225
Eco47I GGWCC 2 cut(s) 7, 215
Eco52I CGGCCG 1 cut(s) 898
Eco57I CTGAAG 2 cut(s) 86, 795
EcoT14I CCWWGG 3 cut(s) 166, 651, 668
EcoT38I GRGCYC 1 cut(s) 752
ErhI CCWWGG 3 cut(s) 166, 651, 668
FaeI CATG 5 cut(s) 170, 258, 386, 523, 748
FaqI GGGAC 2 cut(s) 228, 624
FatI CATG 5 cut(s) 166, 254, 382, 519, 744
FblI GTMKAC 1 cut(s) 580
Fnu4HI GCNGC 1 cut(s) 864
FriOI GRGCYC 1 cut(s) 752
Fsp4HI GCNGC 1 cut(s) 864
FspBI CTAG 1 cut(s) 306
GluI GCNGC 1 cut(s) 864
HaeIII GGCC 2 cut(s) 165, 900
HapII CCGG 2 cut(s) 10, 535
Hin1II CATG 5 cut(s) 170, 258, 386, 523, 748
HincII GTYRAC 1 cut(s) 689
HindII GTYRAC 1 cut(s) 689
HinfI GANTC 1 cut(s) 906
HpaII CCGG 2 cut(s) 10, 535
HphI GGTGA 4 cut(s) 419, 518, 616, 688
Hpy166II GTNNAC 3 cut(s) 526, 581, 689
Hpy188I TCNGA 4 cut(s) 158, 295, 426, 478
Hpy188III TCNNGA 2 cut(s) 321, 842
Hpy8I GTNNAC 3 cut(s) 526, 581, 689
HpyAV CCTTC 5 cut(s) 205, 244, 363, 371, 667
HpyCH4IV ACGT 3 cut(s) 69, 546, 583
HpyCH4V TGCA 7 cut(s) 65, 258, 274, 362, 413, 517, 848
HpyF10VI GCNNNNNNNGC 4 cut(s) 10, 311, 557, 872
HpySE526I ACGT 3 cut(s) 69, 546, 583
Hsp92II CATG 5 cut(s) 170, 258, 386, 523, 748
Kzo9I GATC 6 cut(s) 83, 331, 416, 426, 470, 664
LmnI GCTCC 2 cut(s) 44, 204
LpnPI CCDG 9 cut(s) 23, 121, 147, 211, 231, 399, 548, 708, 726
LweI GCATC 2 cut(s) 400, 835
MaeI CTAG 1 cut(s) 306
MaeII ACGT 3 cut(s) 69, 546, 583
MaeIII GTNAC 1 cut(s) 604
MalI GATC 6 cut(s) 85, 333, 418, 428, 472, 666
MboI GATC 6 cut(s) 83, 331, 416, 426, 470, 664
MboII GAAGA 6 cut(s) 92, 119, 188, 382, 550, 788
MflI RGATCY 1 cut(s) 470
MhlI GDGCHC 2 cut(s) 225, 752
MlsI TGGCCA 1 cut(s) 165
MluCI AATT 7 cut(s) 190, 269, 286, 420, 512, 573, 731
MluNI TGGCCA 1 cut(s) 165
MmeI TCCRAC 1 cut(s) 273
MnlI CCTC 9 cut(s) 108, 397, 458, 484, 575, 772, 774, 896, 900
Mox20I TGGCCA 1 cut(s) 165
MscI TGGCCA 1 cut(s) 165
MseI TTAA 4 cut(s) 44, 237, 336, 489
Msp20I TGGCCA 1 cut(s) 165
MspCI CTTAAG 1 cut(s) 236
MspI CCGG 2 cut(s) 10, 535
Mva1269I GAATGC 1 cut(s) 560
MwoI GCNNNNNNNGC 4 cut(s) 10, 311, 557, 872
NcoI CCATGG 1 cut(s) 166
NdeII GATC 6 cut(s) 83, 331, 416, 426, 470, 664
NlaIII CATG 5 cut(s) 170, 258, 386, 523, 748
NlaIV GGNNCC 1 cut(s) 216
NmeAIII GCCGAG 1 cut(s) 926
NmuCI GTSAC 1 cut(s) 604
NspI RCATGY 1 cut(s) 258
PaeI GCATGC 1 cut(s) 258
PctI GAATGC 1 cut(s) 560
PfeI GAWTC 1 cut(s) 906
PflFI GACNNNGTC 1 cut(s) 585
PflMI CCANNNNNTGG 1 cut(s) 172
PkrI GCNGC 1 cut(s) 865
PshBI ATTAAT 1 cut(s) 489
PspN4I GGNNCC 1 cut(s) 216
PspPI GGNCC 2 cut(s) 7, 215
PsuI RGATCY 1 cut(s) 470
PsyI GACNNNGTC 1 cut(s) 585
RsaI GTAC 3 cut(s) 134, 636, 807
RsaNI GTAC 3 cut(s) 133, 635, 806
SaqAI TTAA 4 cut(s) 44, 237, 336, 489
SatI GCNGC 1 cut(s) 864
Sau3AI GATC 6 cut(s) 83, 331, 416, 426, 470, 664
Sau96I GGNCC 2 cut(s) 7, 215
SduI GDGCHC 2 cut(s) 225, 752
SfaNI GCATC 2 cut(s) 400, 835
SfcI CTRYAG 1 cut(s) 552
SinI GGWCC 2 cut(s) 7, 215
SmlI CTYRAG 2 cut(s) 236, 319
SmoI CTYRAG 2 cut(s) 236, 319
SphI GCATGC 1 cut(s) 258
Sse9I AATT 7 cut(s) 190, 269, 286, 420, 512, 573, 731
SsiI CCGC 1 cut(s) 863
SspMI CTAG 1 cut(s) 306
StyI CCWWGG 3 cut(s) 166, 651, 668
TaiI ACGT 3 cut(s) 72, 549, 586
TaqI TCGA 1 cut(s) 818
TasI AATT 7 cut(s) 190, 269, 286, 420, 512, 573, 731
TatI WGTACW 2 cut(s) 132, 634
TauI GCSGC 1 cut(s) 866
TfiI GAWTC 1 cut(s) 906
Tru1I TTAA 4 cut(s) 44, 237, 336, 489
Tru9I TTAA 4 cut(s) 44, 237, 336, 489
TscAI CASTG 2 cut(s) 230, 442
TseFI GTSAC 1 cut(s) 604
Tsp45I GTSAC 1 cut(s) 604
TspDTI ATGAA 4 cut(s) 104, 383, 390, 475
TspRI CASTG 2 cut(s) 230, 442
Tth111I GACNNNGTC 1 cut(s) 585
Van91I CCANNNNNTGG 1 cut(s) 172
Vha464I CTTAAG 1 cut(s) 236
VpaK11BI GGWCC 2 cut(s) 7, 215
VspI ATTAAT 1 cut(s) 489
XapI RAATTY 2 cut(s) 286, 731
XceI RCATGY 1 cut(s) 258
XmiI GTMKAC 1 cut(s) 580
XspI CTAG 1 cut(s) 306
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.