Rorug03G0111600

Bifunctional monodehydroascorbate reductase and carbonic anhydrase nectarin-3-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0001343
Physical Location & Seq
Reverse (-)
9009710 .. 9012906
3197 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0111600.1

Sequence Viewer

Length: 846 bp
ATGGATCCGACTCCGAACGCTTCGCCGGTCAACGCTCCGAACGAGGACTTCGACCCGGAGCTCCGACCCGGCGGGATGTGCGTCCAGCGCCGCGACTATGACGACGACTCCGCCGCCCGTGGGCCCAACATCACGATCCGCGTCTATCACGGCTCGGCCCACCACGTCATCAACCTCCCGGCCCACTCCACTTTCGGGGAATTGAAGGGACTTGTTTCAGAGAAGACCGGGGTGAAGCCGGAGGAGCAGAAGCTGTTGTTCAGGGGGAAAGAGAAGGAGGATGAGGAGCATTTAGACGCGGCCGGTGTGAGGGATAATTCCAGGGTTATGCTCTTGCAGGAACCGAAAAGGAAAGAGAGGAACGTTGGGGAGGTGAATGAGAGCAATGAGATGTTGAGTGATGCAGCAGAGGCAAAGGCATTTGAAGCAATTGCTGGAGTCAGAGCCGAGGTTGACAGCCTCGCAGAGGGGGTTTCTGCTTTGGGTGTGGTTGTCAATGGTGGAACCAAGGTTTCCGATAAGGATTTTGCTGTGTCGACAGAGTTGCTCATGAGGCAGTTGCTGAAACTGGATGGCATCAAAGCGGAAGGAGAAGCTAGGATGCAGCGGAAGGCTGAGGTTTGCATCTTTAGCATTGCAGTTTCTGATTTGTCAAGTTTGACTTTCCATCATTTTGATCCTCAAGTTGTTAGTTTTTGTGTCTGTTGTTTGATCTCATGGAAAATAATGTATTGTGTTTTATGCATCATACACCTAATCTTTTGTAACAAAATTTTTTCACCTGCTGGGATGTGGTGGATTCTTGCATCCATTGATTGCGTTGACCACATGATGAAGTCAACATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00910 map00910
Pfam Domains
Protein Families
Carb_anhydrase

Protein Analysis

281

Amino Acids

31.02

Weight (kDa)

5.33

Isoelectric Point (pI)

32.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ubiquitin PF00240 44 - 115 1.7e-11 Ubiquitin family
BAG PF02179 144 - 207 1.9e-11 BAG domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 790
Acc36I ACCTGC 1 cut(s) 790
AccI GTMKAC 1 cut(s) 536
AccII CGCG 3 cut(s) 93, 141, 299
AciI CCGC 8 cut(s) 72, 91, 111, 114, 139, 299, 584, 607
AclI AACGTT 1 cut(s) 363
AclWI GGATC 3 cut(s) 12, 130, 671
AcoI YGGCCR 1 cut(s) 300
AcsI RAATTY 1 cut(s) 771
AfiI CCNNNNNNNGG 4 cut(s) 120, 195, 309, 466
AgsI TTSAA 2 cut(s) 205, 425
AjiI CACGTC 1 cut(s) 166
AjnI CCWGG 1 cut(s) 320
AluBI AGCT 3 cut(s) 61, 253, 596
AluI AGCT 3 cut(s) 61, 253, 596
Alw21I GWGCWC 1 cut(s) 63
AlwI GGATC 3 cut(s) 12, 130, 671
AlwNI CAGNNNCTG 3 cut(s) 253, 562, 644
AoxI GGCC 4 cut(s) 122, 156, 180, 300
ApaI GGGCCC 1 cut(s) 126
ApeKI GCWGC 2 cut(s) 404, 604
ApoI RAATTY 1 cut(s) 771
AspLEI GCGC 1 cut(s) 90
AspS9I GGNCC 4 cut(s) 122, 123, 157, 181
AsuC2I CCSGG 4 cut(s) 56, 69, 179, 229
AsuHPI GGTGA 3 cut(s) 244, 385, 771
BaeGI GKGCMC 1 cut(s) 126
BamHI GGATCC 1 cut(s) 4
BanII GRGCYC 2 cut(s) 63, 126
BbsI GAAGAC 1 cut(s) 230
Bbv12I GWGCWC 1 cut(s) 63
BbvCI CCTCAGC 1 cut(s) 615
BbvI GCAGC 2 cut(s) 416, 616
BccI CCATC 2 cut(s) 566, 675
BceAI ACGGC 1 cut(s) 166
BcgI CGANNNNNNTGC 2 cut(s) 526, 560
BciT130I CCWGG 1 cut(s) 322
BcnI CCSGG 4 cut(s) 56, 69, 179, 229
BfaI CTAG 1 cut(s) 597
BfoI RGCGCY 1 cut(s) 91
BfuAI ACCTGC 1 cut(s) 790
BisI GCNGC 5 cut(s) 91, 114, 300, 405, 605
BlsI GCNGC 5 cut(s) 92, 115, 301, 406, 606
Bme1390I CCNGG 5 cut(s) 56, 69, 179, 229, 322
BmgBI CACGTC 1 cut(s) 166
BmgT120I GGNCC 4 cut(s) 122, 123, 157, 181
BmiI GGNNCC 4 cut(s) 6, 124, 342, 505
BmrFI CCNGG 5 cut(s) 56, 69, 179, 229, 322
BmsI GCATC 6 cut(s) 391, 585, 591, 633, 753, 815
BpiI GAAGAC 1 cut(s) 230
BpmI CTGGAG 1 cut(s) 456
Bpu10I CCTNAGC 1 cut(s) 615
BpuEI CTTGAG 1 cut(s) 666
BpuMI CCSGG 4 cut(s) 56, 69, 179, 229
BsaJI CCNNGG 5 cut(s) 118, 228, 321, 447, 507
BsaXI ACNNNNNCTCC 2 cut(s) 92, 122
Bsc4I CCNNNNNNNGG 4 cut(s) 120, 195, 309, 466
Bse118I RCCGGY 2 cut(s) 25, 302
Bse1I ACTGG 1 cut(s) 573
Bse3DI GCAATG 2 cut(s) 391, 633
BseBI CCWGG 1 cut(s) 322
BseDI CCNNGG 5 cut(s) 118, 228, 321, 447, 507
BseGI GGATG 6 cut(s) 81, 286, 577, 606, 795, 806
BseLI CCNNNNNNNGG 4 cut(s) 120, 195, 309, 466
BseMI GCAATG 2 cut(s) 391, 633
BseMII CTCAG 1 cut(s) 606
BseNI ACTGG 1 cut(s) 573
BseRI GAGGAG 2 cut(s) 257, 299
BseSI GKGCMC 1 cut(s) 126
BseX3I CGGCCG 1 cut(s) 300
BseXI GCAGC 2 cut(s) 416, 616
BseYI CCCAGC 1 cut(s) 785
Bsh1236I CGCG 3 cut(s) 93, 141, 299
Bsh1285I CGRYCG 1 cut(s) 303
BshFI GGCC 4 cut(s) 124, 158, 182, 302
BsiEI CGRYCG 1 cut(s) 303
BsiHKAI GWGCWC 1 cut(s) 63
BsiSI CCGG 7 cut(s) 26, 56, 69, 179, 228, 239, 303
BslFI GGGAC 1 cut(s) 222
BslI CCNNNNNNNGG 4 cut(s) 120, 195, 309, 466
BsmFI GGGAC 1 cut(s) 222
BsnI GGCC 4 cut(s) 124, 158, 182, 302
Bsp120I GGGCCC 1 cut(s) 122
Bsp1286I GDGCHC 2 cut(s) 63, 126
Bsp143I GATC 4 cut(s) 4, 135, 676, 711
BspACI CCGC 8 cut(s) 72, 91, 111, 114, 139, 299, 584, 607
BspANI GGCC 4 cut(s) 124, 158, 182, 302
BspCNI CTCAG 1 cut(s) 607
BspFNI CGCG 3 cut(s) 93, 141, 299
BspHI TCATGA 1 cut(s) 549
BspLI GGNNCC 4 cut(s) 6, 124, 342, 505
BspMI ACCTGC 1 cut(s) 790
BspPI GGATC 3 cut(s) 12, 130, 671
BsrDI GCAATG 2 cut(s) 391, 633
BsrFI RCCGGY 2 cut(s) 25, 302
BsrI ACTGG 1 cut(s) 573
BssAI RCCGGY 2 cut(s) 25, 302
BssECI CCNNGG 5 cut(s) 118, 228, 321, 447, 507
BssMI GATC 4 cut(s) 4, 135, 676, 711
BssT1I CCWWGG 1 cut(s) 507
Bst2UI CCWGG 1 cut(s) 322
BstDEI CTNAG 1 cut(s) 615
BstDSI CCRYGG 1 cut(s) 118
BstENI CCTNNNNNAGG 1 cut(s) 464
BstF5I GGATG 6 cut(s) 81, 286, 577, 606, 795, 806
BstFNI CGCG 3 cut(s) 93, 141, 299
BstH2I RGCGCY 1 cut(s) 91
BstHHI GCGC 1 cut(s) 90
BstKTI GATC 4 cut(s) 7, 138, 679, 714
BstMBI GATC 4 cut(s) 4, 135, 676, 711
BstMCI CGRYCG 1 cut(s) 303
BstMWI GCNNNNNNNGC 7 cut(s) 78, 87, 244, 410, 425, 553, 630
BstNI CCWGG 1 cut(s) 322
BstSCI CCNGG 5 cut(s) 54, 67, 177, 227, 320
BstSLI GKGCMC 1 cut(s) 126
BstUI CGCG 3 cut(s) 93, 141, 299
BstV1I GCAGC 2 cut(s) 416, 616
BstV2I GAAGAC 1 cut(s) 230
BstX2I RGATCY 1 cut(s) 4
BstYI RGATCY 1 cut(s) 4
BstZI CGGCCG 1 cut(s) 300
BsuRI GGCC 4 cut(s) 124, 158, 182, 302
BtgI CCRYGG 1 cut(s) 118
BtrI CACGTC 1 cut(s) 166
BtsCI GGATG 6 cut(s) 81, 286, 577, 606, 795, 806
BveI ACCTGC 1 cut(s) 790
CaiI CAGNNNCTG 3 cut(s) 253, 562, 644
CciI TCATGA 1 cut(s) 549
CfoI GCGC 1 cut(s) 90
Cfr10I RCCGGY 2 cut(s) 25, 302
Cfr13I GGNCC 4 cut(s) 122, 123, 157, 181
CseI GACGC 3 cut(s) 70, 130, 305
CviAII CATG 4 cut(s) 550, 717, 829, 843
DdeI CTNAG 1 cut(s) 615
DpnI GATC 4 cut(s) 6, 137, 678, 713
DpnII GATC 4 cut(s) 4, 135, 676, 711
EaeI YGGCCR 1 cut(s) 300
EagI CGGCCG 1 cut(s) 300
EciI GGCGGA 1 cut(s) 100
Ecl136II GAGCTC 1 cut(s) 61
EclXI CGGCCG 1 cut(s) 300
Eco130I CCWWGG 1 cut(s) 507
Eco24I GRGCYC 2 cut(s) 63, 126
Eco52I CGGCCG 1 cut(s) 300
Eco53kI GAGCTC 1 cut(s) 61
EcoICRI GAGCTC 1 cut(s) 61
EcoNI CCTNNNNNAGG 1 cut(s) 464
EcoRII CCWGG 1 cut(s) 320
EcoT14I CCWWGG 1 cut(s) 507
EcoT22I ATGCAT 1 cut(s) 746
EcoT38I GRGCYC 2 cut(s) 63, 126
ErhI CCWWGG 1 cut(s) 507
FaeI CATG 4 cut(s) 553, 720, 832, 846
FaiI YATR 8 cut(s) 99, 329, 551, 718, 742, 749, 830, 844
FalI AAGNNNNNCTT 2 cut(s) 646, 678
FaqI GGGAC 1 cut(s) 222
FatI CATG 4 cut(s) 549, 716, 828, 842
FauI CCCGC 1 cut(s) 65
FblI GTMKAC 1 cut(s) 536
Fnu4HI GCNGC 5 cut(s) 91, 114, 300, 405, 605
FokI GGATG 6 cut(s) 88, 293, 584, 613, 793, 802
FriOI GRGCYC 2 cut(s) 63, 126
Fsp4HI GCNGC 5 cut(s) 91, 114, 300, 405, 605
FspBI CTAG 1 cut(s) 597
GlaI GCGC 1 cut(s) 89
GluI GCNGC 5 cut(s) 91, 114, 300, 405, 605
GsaI CCCAGC 1 cut(s) 789
GsuI CTGGAG 1 cut(s) 456
HaeII RGCGCY 1 cut(s) 91
HaeIII GGCC 4 cut(s) 124, 158, 182, 302
HapII CCGG 7 cut(s) 26, 56, 69, 179, 228, 239, 303
HgaI GACGC 3 cut(s) 70, 130, 305
HhaI GCGC 1 cut(s) 90
Hin1II CATG 4 cut(s) 553, 720, 832, 846
Hin6I GCGC 1 cut(s) 88
HinP1I GCGC 1 cut(s) 88
HincII GTYRAC 5 cut(s) 31, 454, 537, 823, 840
HindII GTYRAC 5 cut(s) 31, 454, 537, 823, 840
HinfI GANTC 4 cut(s) 10, 107, 438, 799
HpaII CCGG 7 cut(s) 26, 56, 69, 179, 228, 239, 303
HphI GGTGA 3 cut(s) 244, 385, 771
Hpy166II GTNNAC 5 cut(s) 31, 454, 537, 823, 840
Hpy188I TCNGA 8 cut(s) 9, 15, 39, 65, 220, 443, 517, 646
Hpy188III TCNNGA 2 cut(s) 133, 550
Hpy8I GTNNAC 5 cut(s) 31, 454, 537, 823, 840
Hpy99I CGWCG 1 cut(s) 107
HpyAV CCTTC 4 cut(s) 199, 268, 581, 604
HpyCH4IV ACGT 2 cut(s) 165, 363
HpyCH4V TGCA 7 cut(s) 337, 404, 604, 624, 638, 744, 806
HpyF10VI GCNNNNNNNGC 7 cut(s) 78, 87, 244, 410, 425, 553, 630
HpyF3I CTNAG 1 cut(s) 615
HpySE526I ACGT 2 cut(s) 165, 363
Hsp92II CATG 4 cut(s) 553, 720, 832, 846
HspAI GCGC 1 cut(s) 88
Kzo9I GATC 4 cut(s) 4, 135, 676, 711
LmnI GCTCC 5 cut(s) 40, 58, 66, 244, 286
Lsp1109I GCAGC 2 cut(s) 416, 616
LweI GCATC 6 cut(s) 391, 585, 591, 633, 753, 815
MaeI CTAG 1 cut(s) 597
MaeII ACGT 2 cut(s) 165, 363
MaeIII GTNAC 1 cut(s) 764
MalI GATC 4 cut(s) 6, 137, 678, 713
MboI GATC 4 cut(s) 4, 135, 676, 711
MboII GAAGA 1 cut(s) 235
MfeI CAATTG 1 cut(s) 429
MflI RGATCY 1 cut(s) 4
MhlI GDGCHC 2 cut(s) 63, 126
MluCI AATT 4 cut(s) 200, 316, 429, 771
MlyI GAGTC 3 cut(s) 4, 101, 447
MmeI TCCRAC 2 cut(s) 32, 88
Mph1103I ATGCAT 1 cut(s) 746
MspA1I CMGCKG 1 cut(s) 607
MspI CCGG 7 cut(s) 26, 56, 69, 179, 228, 239, 303
MspR9I CCNGG 5 cut(s) 56, 69, 179, 229, 322
MunI CAATTG 1 cut(s) 429
MvaI CCWGG 1 cut(s) 322
MvnI CGCG 3 cut(s) 93, 141, 299
MwoI GCNNNNNNNGC 7 cut(s) 78, 87, 244, 410, 425, 553, 630
NciI CCSGG 4 cut(s) 56, 69, 179, 229
NdeII GATC 4 cut(s) 4, 135, 676, 711
NlaIII CATG 4 cut(s) 553, 720, 832, 846
NlaIV GGNNCC 4 cut(s) 6, 124, 342, 505
NmeAIII GCCGAG 2 cut(s) 134, 472
NsiI ATGCAT 1 cut(s) 746
PagI TCATGA 1 cut(s) 549
PaqCI CACCTGC 1 cut(s) 790
PcsI WCGNNNNNNNCGW 2 cut(s) 39, 48
PfeI GAWTC 1 cut(s) 799
PkrI GCNGC 5 cut(s) 92, 115, 301, 406, 606
PleI GAGTC 3 cut(s) 4, 101, 446
PpsI GAGTC 3 cut(s) 4, 101, 446
Psp124BI GAGCTC 1 cut(s) 63
Psp1406I AACGTT 1 cut(s) 363
Psp6I CCWGG 1 cut(s) 320
PspFI CCCAGC 1 cut(s) 785
PspGI CCWGG 1 cut(s) 320
PspN4I GGNNCC 4 cut(s) 6, 124, 342, 505
PspOMI GGGCCC 1 cut(s) 122
PspPI GGNCC 4 cut(s) 122, 123, 157, 181
PstNI CAGNNNCTG 3 cut(s) 253, 562, 644
PsuI RGATCY 1 cut(s) 4
SacI GAGCTC 1 cut(s) 63
SalI GTCGAC 1 cut(s) 535
SatI GCNGC 5 cut(s) 91, 114, 300, 405, 605
Sau3AI GATC 4 cut(s) 4, 135, 676, 711
Sau96I GGNCC 4 cut(s) 122, 123, 157, 181
SchI GAGTC 3 cut(s) 4, 101, 447
ScrFI CCNGG 5 cut(s) 56, 69, 179, 229, 322
SduI GDGCHC 2 cut(s) 63, 126
SfaNI GCATC 6 cut(s) 391, 585, 591, 633, 753, 815
SmlI CTYRAG 1 cut(s) 681
SmoI CTYRAG 1 cut(s) 681
Sse9I AATT 4 cut(s) 200, 316, 429, 771
SsiI CCGC 8 cut(s) 72, 91, 111, 114, 139, 299, 584, 607
SspMI CTAG 1 cut(s) 597
SstI GAGCTC 1 cut(s) 63
StyD4I CCNGG 5 cut(s) 54, 67, 177, 227, 320
StyI CCWWGG 1 cut(s) 507
TaiI ACGT 2 cut(s) 168, 366
TaqI TCGA 2 cut(s) 51, 536
TasI AATT 4 cut(s) 200, 316, 429, 771
TauI GCSGC 3 cut(s) 93, 116, 302
TfiI GAWTC 1 cut(s) 799
TseI GCWGC 2 cut(s) 404, 604
XagI CCTNNNNNAGG 1 cut(s) 464
XapI RAATTY 1 cut(s) 771
XmiI GTMKAC 1 cut(s) 536
XspI CTAG 1 cut(s) 597
Zsp2I ATGCAT 1 cut(s) 746
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.