Rh3DG187700

Bifunctional monodehydroascorbate reductase and carbonic anhydrase nectarin-3-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3D
OG0001343
Physical Location & Seq
Forward (+)
16725871 .. 16727416
1546 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3DG187700.1

Sequence Viewer

Length: 882 bp
ATGGTTAAATCAAAGAAAGTCCATCTTCAGTTCTTTGCTTTCACTCTTTTTCTTCTGTCCTGGGACCCCATCCAACCCGTCAGAGGCGAAGGAGTGGAAGTAATGGAGTATAATTATAGGGAAGGGAGCCCCAAGGGACCAGAGCACTGGGGAGAGCTCAACGAAGGATTGGAGGAATGCAAAGATGGAAAATTACAATCGCCAATAGATTTGTTCAATTCGAATGCTAGAAAAGCAATCTCAAGAGTAGATGATCTTAACATGAGCTATAAGCCTTCAAAAGCAACTATGAAGCTTGAACACCATGCTATTTCGATTCAATGGGAGGGTGATGCTGGATCAATTCAAATAAATGGCACTGAATACTTGCTCAAAAGATGTCATTGGCACAGATCCTCTGAACATTTCATTAATGGCAGAAGGTTTCTCTTTCTCTTTGCTTCTCTGTTTGTAAAATCCAAAATACTTCATATATGTCTAAACAGAGTTCTTTTTATCAGTTACGATTTGGAATTGCACATGGTTCACCAAAGCCCGAACAACGATGTGGCTGTAGTCGCATTCCTATACCAAAGTGGCAATCCCGATCCTTTCCTGTCAAAGATAAGCAACGATGTATCCTCCCTAAGTGGTACAAAGGAGGCACGCTTGGGAGTGATTCATCCAAGTTTAATAAAATGGCCAAGCTCACGATTTTATAGATATATAGGCTCACTCACGGCCCCTCCTTGCACTGAAGGAGTTATTTGGAGCGTAAGCGAAGAGGTACGTTCTGTTTCAAAAGAACAGCTGGGGCTAATTGAACAAGTCGATCGTGGGATGAATGCAAGACCATTACAGCCTCTCAACAATCGAGACATCACGCTCATTGGTCCGCAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00910 map00910
Pfam Domains
Protein Families
Carb_anhydrase

Protein Analysis

293

Amino Acids

33.32

Weight (kDa)

7.75

Isoelectric Point (pI)

46.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Carb_anhydrase PF00194 45 - 288 3.2e-28 Eukaryotic-type carbonic anhydrase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 875
AclWI GGATC 3 cut(s) 346, 387, 581
AcoI YGGCCR 1 cut(s) 680
AcuI CTGAAG 2 cut(s) 11, 756
AfaI GTAC 2 cut(s) 634, 768
AfiI CCNNNNNNNGG 1 cut(s) 83
AgsI TTSAA 7 cut(s) 217, 279, 299, 320, 347, 780, 803
AjnI CCWGG 1 cut(s) 59
AluBI AGCT 5 cut(s) 157, 267, 295, 687, 790
AluI AGCT 5 cut(s) 157, 267, 295, 687, 790
Alw21I GWGCWC 2 cut(s) 147, 159
Alw26I GTCTC 1 cut(s) 849
AlwI GGATC 3 cut(s) 346, 387, 581
AoxI GGCC 2 cut(s) 680, 720
AseI ATTAAT 1 cut(s) 411
AspS9I GGNCC 4 cut(s) 64, 137, 721, 872
AsuHPI GGTGA 2 cut(s) 341, 518
AsuII TTCGAA 1 cut(s) 221
AvaII GGWCC 3 cut(s) 64, 137, 872
BalI TGGCCA 1 cut(s) 682
BanII GRGCYC 2 cut(s) 131, 159
Bbv12I GWGCWC 2 cut(s) 147, 159
BccI CCATC 3 cut(s) 30, 77, 179
BceAI ACGGC 1 cut(s) 735
BciT130I CCWGG 1 cut(s) 61
BciVI GTATCC 1 cut(s) 628
BcoDI GTCTC 1 cut(s) 849
BfaI CTAG 1 cut(s) 228
BfmI CTRYAG 1 cut(s) 552
BfuI GTATCC 1 cut(s) 628
Bme1390I CCNGG 1 cut(s) 61
Bme18I GGWCC 3 cut(s) 64, 137, 872
BmgT120I GGNCC 4 cut(s) 64, 137, 721, 872
BmiI GGNNCC 5 cut(s) 65, 66, 128, 138, 723
BmrFI CCNGG 1 cut(s) 61
BmrI ACTGGG 1 cut(s) 157
BmsI GCATC 1 cut(s) 322
BmuI ACTGGG 1 cut(s) 157
Bpu14I TTCGAA 1 cut(s) 221
BpuEI CTTGAG 1 cut(s) 226
BsaJI CCNNGG 2 cut(s) 60, 132
BsaXI ACNNNNNCTCC 4 cut(s) 84, 114, 709, 739
Bsc4I CCNNNNNNNGG 1 cut(s) 83
Bse1I ACTGG 1 cut(s) 152
BseBI CCWGG 1 cut(s) 61
BseDI CCNNGG 2 cut(s) 60, 132
BseGI GGATG 3 cut(s) 69, 661, 825
BseLI CCNNNNNNNGG 1 cut(s) 83
BseNI ACTGG 1 cut(s) 152
BseYI CCCAGC 1 cut(s) 790
Bsh1285I CGRYCG 1 cut(s) 814
BshFI GGCC 2 cut(s) 682, 722
BsiEI CGRYCG 1 cut(s) 814
BsiHKAI GWGCWC 2 cut(s) 147, 159
BslFI GGGAC 2 cut(s) 77, 150
BslI CCNNNNNNNGG 1 cut(s) 83
BsmAI GTCTC 1 cut(s) 849
BsmFI GGGAC 2 cut(s) 77, 150
BsmI GAATGC 4 cut(s) 182, 229, 560, 829
BsnI GGCC 2 cut(s) 682, 722
Bsp119I TTCGAA 1 cut(s) 221
Bsp1286I GDGCHC 3 cut(s) 131, 147, 159
Bsp143I GATC 5 cut(s) 253, 338, 392, 586, 811
BspACI CCGC 1 cut(s) 875
BspANI GGCC 2 cut(s) 682, 722
BspLI GGNNCC 5 cut(s) 65, 66, 128, 138, 723
BspPI GGATC 3 cut(s) 346, 387, 581
BspT104I TTCGAA 1 cut(s) 221
BsrI ACTGG 1 cut(s) 152
BssECI CCNNGG 2 cut(s) 60, 132
BssMI GATC 5 cut(s) 253, 338, 392, 586, 811
BssT1I CCWWGG 1 cut(s) 132
Bst2UI CCWGG 1 cut(s) 61
Bst6I CTCTTC 1 cut(s) 756
BstBI TTCGAA 1 cut(s) 221
BstC8I GCNNGC 1 cut(s) 646
BstDEI CTNAG 1 cut(s) 626
BstF5I GGATG 3 cut(s) 69, 661, 825
BstKTI GATC 5 cut(s) 256, 341, 395, 589, 814
BstMAI GTCTC 1 cut(s) 849
BstMBI GATC 5 cut(s) 253, 338, 392, 586, 811
BstMCI CGRYCG 1 cut(s) 814
BstMWI GCNNNNNNNGC 2 cut(s) 233, 557
BstNI CCWGG 1 cut(s) 61
BstSCI CCNGG 1 cut(s) 59
BstSFI CTRYAG 1 cut(s) 552
BstX2I RGATCY 1 cut(s) 392
BstXI CCANNNNNNTGG 1 cut(s) 147
BstYI RGATCY 1 cut(s) 392
BsuI GTATCC 1 cut(s) 628
BsuRI GGCC 2 cut(s) 682, 722
BtsCI GGATG 3 cut(s) 69, 661, 825
BtsIMutI CAGTG 3 cut(s) 145, 357, 732
Cac8I GCNNGC 1 cut(s) 646
Cfr13I GGNCC 4 cut(s) 64, 137, 721, 872
Csp6I GTAC 2 cut(s) 633, 767
CviAII CATG 3 cut(s) 262, 305, 520
CviQI GTAC 2 cut(s) 633, 767
DdeI CTNAG 1 cut(s) 626
DpnI GATC 5 cut(s) 255, 340, 394, 588, 813
DpnII GATC 5 cut(s) 253, 338, 392, 586, 811
EaeI YGGCCR 1 cut(s) 680
Eam1104I CTCTTC 1 cut(s) 756
EarI CTCTTC 1 cut(s) 756
Ecl136II GAGCTC 1 cut(s) 157
Eco130I CCWWGG 1 cut(s) 132
Eco24I GRGCYC 2 cut(s) 131, 159
Eco47I GGWCC 3 cut(s) 64, 137, 872
Eco53kI GAGCTC 1 cut(s) 157
Eco57I CTGAAG 2 cut(s) 11, 756
EcoICRI GAGCTC 1 cut(s) 157
EcoO109I RGGNCCY 1 cut(s) 64
EcoRII CCWGG 1 cut(s) 59
EcoT14I CCWWGG 1 cut(s) 132
EcoT38I GRGCYC 2 cut(s) 131, 159
ErhI CCWWGG 1 cut(s) 132
FaeI CATG 3 cut(s) 265, 308, 523
FalI AAGNNNNNCTT 2 cut(s) 9, 41
FaqI GGGAC 2 cut(s) 77, 150
FatI CATG 3 cut(s) 261, 304, 519
FokI GGATG 3 cut(s) 56, 648, 832
FriOI GRGCYC 2 cut(s) 131, 159
FspBI CTAG 1 cut(s) 228
GsaI CCCAGC 1 cut(s) 794
HaeIII GGCC 2 cut(s) 682, 722
Hin1II CATG 3 cut(s) 265, 308, 523
HindIII AAGCTT 1 cut(s) 293
HinfI GANTC 2 cut(s) 316, 658
HphI GGTGA 2 cut(s) 341, 518
Hpy166II GTNNAC 1 cut(s) 526
Hpy188I TCNGA 2 cut(s) 83, 400
Hpy188III TCNNGA 4 cut(s) 243, 584, 690, 854
Hpy8I GTNNAC 1 cut(s) 526
HpyAV CCTTC 6 cut(s) 83, 116, 158, 285, 414, 731
HpyCH4IV ACGT 1 cut(s) 769
HpyCH4V TGCA 4 cut(s) 180, 517, 732, 827
HpyF10VI GCNNNNNNNGC 2 cut(s) 233, 557
HpyF3I CTNAG 1 cut(s) 626
HpySE526I ACGT 1 cut(s) 769
Hsp92II CATG 3 cut(s) 265, 308, 523
KflI GGGWCCC 1 cut(s) 64
Kzo9I GATC 5 cut(s) 253, 338, 392, 586, 811
LmnI GCTCC 2 cut(s) 126, 750
LpnPI CCDG 7 cut(s) 46, 73, 133, 153, 321, 608, 776
LweI GCATC 1 cut(s) 322
MaeI CTAG 1 cut(s) 228
MaeII ACGT 1 cut(s) 769
MaeIII GTNAC 1 cut(s) 500
MalI GATC 5 cut(s) 255, 340, 394, 588, 813
MboI GATC 5 cut(s) 253, 338, 392, 586, 811
MboII GAAGA 3 cut(s) 17, 44, 773
MflI RGATCY 1 cut(s) 392
MhlI GDGCHC 3 cut(s) 131, 147, 159
MlsI TGGCCA 1 cut(s) 682
MluCI AATT 6 cut(s) 112, 191, 217, 342, 512, 798
MluNI TGGCCA 1 cut(s) 682
MmeI TCCRAC 1 cut(s) 97
MnlI CCTC 9 cut(s) 77, 166, 319, 406, 631, 634, 735, 757, 852
Mox20I TGGCCA 1 cut(s) 682
MscI TGGCCA 1 cut(s) 682
MseI TTAA 4 cut(s) 6, 258, 411, 671
Msp20I TGGCCA 1 cut(s) 682
MspA1I CMGCKG 1 cut(s) 790
MspR9I CCNGG 1 cut(s) 61
Mva1269I GAATGC 4 cut(s) 182, 229, 560, 829
MvaI CCWGG 1 cut(s) 61
MwoI GCNNNNNNNGC 2 cut(s) 233, 557
NdeII GATC 5 cut(s) 253, 338, 392, 586, 811
NlaIII CATG 3 cut(s) 265, 308, 523
NlaIV GGNNCC 5 cut(s) 65, 66, 128, 138, 723
NspV TTCGAA 1 cut(s) 221
PctI GAATGC 4 cut(s) 182, 229, 560, 829
PfeI GAWTC 2 cut(s) 316, 658
Ple19I CGATCG 1 cut(s) 814
PpuMI RGGWCCY 1 cut(s) 64
PshBI ATTAAT 1 cut(s) 411
Psp124BI GAGCTC 1 cut(s) 159
Psp5II RGGWCCY 1 cut(s) 64
Psp6I CCWGG 1 cut(s) 59
PspFI CCCAGC 1 cut(s) 790
PspGI CCWGG 1 cut(s) 59
PspN4I GGNNCC 5 cut(s) 65, 66, 128, 138, 723
PspPI GGNCC 4 cut(s) 64, 137, 721, 872
PspPPI RGGWCCY 1 cut(s) 64
PsuI RGATCY 1 cut(s) 392
PvuI CGATCG 1 cut(s) 814
PvuII CAGCTG 1 cut(s) 790
RsaI GTAC 2 cut(s) 634, 768
RsaNI GTAC 2 cut(s) 633, 767
SacI GAGCTC 1 cut(s) 159
SaqAI TTAA 4 cut(s) 6, 258, 411, 671
Sau3AI GATC 5 cut(s) 253, 338, 392, 586, 811
Sau96I GGNCC 4 cut(s) 64, 137, 721, 872
ScrFI CCNGG 1 cut(s) 61
SduI GDGCHC 3 cut(s) 131, 147, 159
SetI ASST 8 cut(s) 159, 269, 297, 425, 689, 768, 772, 792
SfaNI GCATC 1 cut(s) 322
SfcI CTRYAG 1 cut(s) 552
SfuI TTCGAA 1 cut(s) 221
SinI GGWCC 3 cut(s) 64, 137, 872
SmlI CTYRAG 1 cut(s) 241
SmoI CTYRAG 1 cut(s) 241
Sse9I AATT 6 cut(s) 112, 191, 217, 342, 512, 798
SsiI CCGC 1 cut(s) 875
SspMI CTAG 1 cut(s) 228
SstI GAGCTC 1 cut(s) 159
StyD4I CCNGG 1 cut(s) 59
StyI CCWWGG 1 cut(s) 132
TaiI ACGT 1 cut(s) 772
TaqI TCGA 4 cut(s) 221, 314, 810, 853
TasI AATT 6 cut(s) 112, 191, 217, 342, 512, 798
TfiI GAWTC 2 cut(s) 316, 658
Tru1I TTAA 4 cut(s) 6, 258, 411, 671
Tru9I TTAA 4 cut(s) 6, 258, 411, 671
TscAI CASTG 3 cut(s) 152, 364, 739
TspDTI ATGAA 5 cut(s) 305, 397, 458, 650, 836
TspRI CASTG 3 cut(s) 152, 364, 739
VpaK11BI GGWCC 3 cut(s) 64, 137, 872
VspI ATTAAT 1 cut(s) 411
XspI CTAG 1 cut(s) 228
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.