Rh3CG178200

Bifunctional monodehydroascorbate reductase and carbonic anhydrase nectarin-3-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3C
OG0001343
Physical Location & Seq
Reverse (-)
15661417 .. 15675319
13903 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3CG178200.1

Sequence Viewer

Length: 996 bp
ATGGGAAGGCCAATGCCTTTAGAGTCCAATTTGATAGCAAGTAGAGAGTTGCAGAAGCCATCTCATCAGAATAATGAAGTTGCTGACCAGACCAGTCCCCCATTGGAGGAAGTCGACCTTGGTGAGAAGCAAAAGACCCAAGTCCCAAAGATCAACAACGTGTACACTCAAGACGTAGTGATGGTTAAATCAAAGAAAGTCCATCTTCAGTTGTTTGCTTTCACTCTTTTTCTTCTGTCCTGGCACCCCATCAAACGCGTCAGAGGCGAAGGAGTGGAAGTAATGGAGTATAATTATAGGGAAGGGAGCCCCAAGGGACCAGAGCACTGGGGAGAGCTCAACGAAGAAATGGAGAAATGCAAAGATGGAAAATTACAATCGCCAATAGATTTGTTCGATTCGAATGCTAGAAAAGCAATCTCAAGAGTAGATGATCTTAACATGAGCTATAACCCTTCAAAAGCAACTATGAAGCTTGAACACCATGCTATTTCGATTCAATGGGAGGGTGATGCTGGATCAATTCAAATAAATGGCACTGAATACTTGCTCAAAAGATGTCATTGGCACAGATCCTCTGAACATTTCATTAATGGCAGAAGGTTTCTCTTTCTCTTTGCTTCTCTGTTTGTAAAATCCAAAATACTTCATATATGTCTAAACAGAGTTTTTTTTATCAGTTATGATTTGGAATTGCACATGGTTCACCAAAGCCAGAACAACGATGTGGCTGTAGTCGCATTCCTATACCAAAGTGGCAATCCCGATCCTTTCCTGTCAAAGATAAGCAACGATGTATCCTCCCTAAGTGTTACAAAGGAGGCACGCTTGGGAGTGATTCATCCAAGTTTAATAAAATGGCCAAGCTCGCGATTTTATAGATATATAGGCTCACTCACGGCCCCTCCTTGCACTGAAGGAGTTATTTGGAGCGTAAGCGAAGAGGTACGTTCTGTTTCAAAAGAACAGCTGGGGCTAATTGAACAAGTCGATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00910 map00910
Pfam Domains
Protein Families
Carb_anhydrase

Protein Analysis

331

Amino Acids

37.82

Weight (kDa)

6.68

Isoelectric Point (pI)

46.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Carb_anhydrase PF00194 105 - 329 2e-25 Eukaryotic-type carbonic anhydrase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 243
AccI GTMKAC 1 cut(s) 114
AccII CGCG 2 cut(s) 258, 871
AclWI GGATC 3 cut(s) 526, 567, 761
AcoI YGGCCR 1 cut(s) 860
AcuI CTGAAG 2 cut(s) 191, 936
AfaI GTAC 2 cut(s) 164, 948
AfiI CCNNNNNNNGG 1 cut(s) 106
AflIII ACRYGT 2 cut(s) 159, 256
AgsI TTSAA 6 cut(s) 459, 479, 500, 527, 960, 983
AjnI CCWGG 1 cut(s) 239
AjuI GAANNNNNNNTTGG 2 cut(s) 102, 134
AluBI AGCT 5 cut(s) 337, 447, 475, 867, 970
AluI AGCT 5 cut(s) 337, 447, 475, 867, 970
Alw21I GWGCWC 2 cut(s) 327, 339
AlwI GGATC 3 cut(s) 526, 567, 761
AoxI GGCC 3 cut(s) 8, 860, 900
AseI ATTAAT 1 cut(s) 591
AspS9I GGNCC 2 cut(s) 317, 901
AsuHPI GGTGA 3 cut(s) 134, 521, 698
AsuII TTCGAA 1 cut(s) 401
AvaII GGWCC 1 cut(s) 317
BalI TGGCCA 1 cut(s) 862
BanI GGYRCC 1 cut(s) 243
BanII GRGCYC 2 cut(s) 311, 339
Bbv12I GWGCWC 2 cut(s) 327, 339
BccI CCATC 5 cut(s) 67, 175, 210, 257, 359
BceAI ACGGC 1 cut(s) 915
BcgI CGANNNNNNTGC 2 cut(s) 386, 420
BciT130I CCWGG 1 cut(s) 241
BciVI GTATCC 1 cut(s) 808
BfaI CTAG 1 cut(s) 408
BfmI CTRYAG 1 cut(s) 732
BfuI GTATCC 1 cut(s) 808
Bme1390I CCNGG 1 cut(s) 241
Bme18I GGWCC 1 cut(s) 317
BmgT120I GGNCC 2 cut(s) 317, 901
BmiI GGNNCC 4 cut(s) 245, 308, 318, 903
BmrFI CCNGG 1 cut(s) 241
BmrI ACTGGG 1 cut(s) 337
BmsI GCATC 1 cut(s) 502
BmuI ACTGGG 1 cut(s) 337
BoxI GACNNNNGTC 1 cut(s) 140
Bpu14I TTCGAA 1 cut(s) 401
BpuEI CTTGAG 2 cut(s) 153, 406
BsaJI CCNNGG 2 cut(s) 118, 312
BsaXI ACNNNNNCTCC 4 cut(s) 264, 294, 889, 919
Bsc4I CCNNNNNNNGG 1 cut(s) 106
Bse1I ACTGG 2 cut(s) 93, 332
BseBI CCWGG 1 cut(s) 241
BseDI CCNNGG 2 cut(s) 118, 312
BseGI GGATG 1 cut(s) 841
BseLI CCNNNNNNNGG 1 cut(s) 106
BseNI ACTGG 2 cut(s) 93, 332
BseYI CCCAGC 1 cut(s) 970
Bsh1236I CGCG 2 cut(s) 258, 871
BshFI GGCC 3 cut(s) 10, 862, 902
BshNI GGYRCC 1 cut(s) 243
BsiHKAI GWGCWC 2 cut(s) 327, 339
BslFI GGGAC 3 cut(s) 81, 128, 330
BslI CCNNNNNNNGG 1 cut(s) 106
BsmFI GGGAC 3 cut(s) 81, 128, 330
BsmI GAATGC 2 cut(s) 409, 740
BsnI GGCC 3 cut(s) 10, 862, 902
Bsp119I TTCGAA 1 cut(s) 401
Bsp1286I GDGCHC 3 cut(s) 311, 327, 339
Bsp1407I TGTACA 1 cut(s) 162
Bsp143I GATC 5 cut(s) 150, 433, 518, 572, 766
Bsp68I TCGCGA 1 cut(s) 871
BspANI GGCC 3 cut(s) 10, 862, 902
BspFNI CGCG 2 cut(s) 258, 871
BspLI GGNNCC 4 cut(s) 245, 308, 318, 903
BspPI GGATC 3 cut(s) 526, 567, 761
BspT104I TTCGAA 1 cut(s) 401
BspT107I GGYRCC 1 cut(s) 243
BsrGI TGTACA 1 cut(s) 162
BsrI ACTGG 2 cut(s) 93, 332
BssECI CCNNGG 2 cut(s) 118, 312
BssMI GATC 5 cut(s) 150, 433, 518, 572, 766
BssT1I CCWWGG 2 cut(s) 118, 312
Bst2UI CCWGG 1 cut(s) 241
Bst6I CTCTTC 1 cut(s) 936
BstAUI TGTACA 1 cut(s) 162
BstBI TTCGAA 1 cut(s) 401
BstC8I GCNNGC 2 cut(s) 826, 869
BstDEI CTNAG 1 cut(s) 806
BstF5I GGATG 1 cut(s) 841
BstFNI CGCG 2 cut(s) 258, 871
BstKTI GATC 5 cut(s) 153, 436, 521, 575, 769
BstMBI GATC 5 cut(s) 150, 433, 518, 572, 766
BstMWI GCNNNNNNNGC 4 cut(s) 264, 413, 737, 868
BstNI CCWGG 1 cut(s) 241
BstPAI GACNNNNGTC 1 cut(s) 140
BstSCI CCNGG 1 cut(s) 239
BstSFI CTRYAG 1 cut(s) 732
BstUI CGCG 2 cut(s) 258, 871
BstX2I RGATCY 1 cut(s) 572
BstXI CCANNNNNNTGG 1 cut(s) 327
BstYI RGATCY 1 cut(s) 572
BsuI GTATCC 1 cut(s) 808
BsuRI GGCC 3 cut(s) 10, 862, 902
BtsCI GGATG 1 cut(s) 841
BtsIMutI CAGTG 3 cut(s) 325, 537, 912
BtuMI TCGCGA 1 cut(s) 871
Cac8I GCNNGC 2 cut(s) 826, 869
Cfr13I GGNCC 2 cut(s) 317, 901
CseI GACGC 1 cut(s) 247
Csp6I GTAC 2 cut(s) 163, 947
CviAII CATG 3 cut(s) 442, 485, 700
CviQI GTAC 2 cut(s) 163, 947
DdeI CTNAG 1 cut(s) 806
DpnI GATC 5 cut(s) 152, 435, 520, 574, 768
DpnII GATC 5 cut(s) 150, 433, 518, 572, 766
EaeI YGGCCR 1 cut(s) 860
Eam1104I CTCTTC 1 cut(s) 936
EarI CTCTTC 1 cut(s) 936
Ecl136II GAGCTC 1 cut(s) 337
Eco130I CCWWGG 2 cut(s) 118, 312
Eco24I GRGCYC 2 cut(s) 311, 339
Eco47I GGWCC 1 cut(s) 317
Eco53kI GAGCTC 1 cut(s) 337
Eco57I CTGAAG 2 cut(s) 191, 936
EcoICRI GAGCTC 1 cut(s) 337
EcoRII CCWGG 1 cut(s) 239
EcoT14I CCWWGG 2 cut(s) 118, 312
EcoT38I GRGCYC 2 cut(s) 311, 339
ErhI CCWWGG 2 cut(s) 118, 312
FaeI CATG 3 cut(s) 445, 488, 703
FalI AAGNNNNNCTT 4 cut(s) 102, 134, 189, 221
FaqI GGGAC 3 cut(s) 81, 128, 330
FatI CATG 3 cut(s) 441, 484, 699
FblI GTMKAC 1 cut(s) 114
FokI GGATG 1 cut(s) 828
FriOI GRGCYC 2 cut(s) 311, 339
FspBI CTAG 1 cut(s) 408
GsaI CCCAGC 1 cut(s) 974
HaeIII GGCC 3 cut(s) 10, 862, 902
HgaI GACGC 1 cut(s) 247
Hin1II CATG 3 cut(s) 445, 488, 703
HincII GTYRAC 1 cut(s) 115
HindII GTYRAC 1 cut(s) 115
HindIII AAGCTT 1 cut(s) 473
HinfI GANTC 4 cut(s) 23, 398, 496, 838
HphI GGTGA 3 cut(s) 134, 521, 698
Hpy166II GTNNAC 4 cut(s) 115, 163, 165, 706
Hpy188I TCNGA 3 cut(s) 69, 263, 580
Hpy188III TCNNGA 4 cut(s) 170, 423, 764, 870
Hpy8I GTNNAC 4 cut(s) 115, 163, 165, 706
HpyAV CCTTC 5 cut(s) 263, 296, 465, 594, 911
HpyCH4IV ACGT 3 cut(s) 159, 174, 949
HpyCH4V TGCA 4 cut(s) 52, 360, 697, 912
HpyF10VI GCNNNNNNNGC 4 cut(s) 264, 413, 737, 868
HpyF3I CTNAG 1 cut(s) 806
HpySE526I ACGT 3 cut(s) 159, 174, 949
Hsp92II CATG 3 cut(s) 445, 488, 703
Kzo9I GATC 5 cut(s) 150, 433, 518, 572, 766
LmnI GCTCC 2 cut(s) 306, 930
LweI GCATC 1 cut(s) 502
MaeI CTAG 1 cut(s) 408
MaeII ACGT 3 cut(s) 159, 174, 949
MaeIII GTNAC 1 cut(s) 811
MalI GATC 5 cut(s) 152, 435, 520, 574, 768
MboI GATC 5 cut(s) 150, 433, 518, 572, 766
MboII GAAGA 4 cut(s) 197, 224, 356, 953
MflI RGATCY 1 cut(s) 572
MhlI GDGCHC 3 cut(s) 311, 327, 339
MlsI TGGCCA 1 cut(s) 862
MluCI AATT 6 cut(s) 28, 292, 371, 522, 692, 978
MluI ACGCGT 1 cut(s) 256
MluNI TGGCCA 1 cut(s) 862
MlyI GAGTC 1 cut(s) 32
MnlI CCTC 8 cut(s) 100, 257, 499, 586, 811, 814, 915, 937
Mox20I TGGCCA 1 cut(s) 862
MscI TGGCCA 1 cut(s) 862
MseI TTAA 4 cut(s) 186, 438, 591, 851
Msp20I TGGCCA 1 cut(s) 862
MspA1I CMGCKG 1 cut(s) 970
MspR9I CCNGG 1 cut(s) 241
Mva1269I GAATGC 2 cut(s) 409, 740
MvaI CCWGG 1 cut(s) 241
MvnI CGCG 2 cut(s) 258, 871
MwoI GCNNNNNNNGC 4 cut(s) 264, 413, 737, 868
NdeII GATC 5 cut(s) 150, 433, 518, 572, 766
NlaIII CATG 3 cut(s) 445, 488, 703
NlaIV GGNNCC 4 cut(s) 245, 308, 318, 903
NruI TCGCGA 1 cut(s) 871
NspV TTCGAA 1 cut(s) 401
PctI GAATGC 2 cut(s) 409, 740
PfeI GAWTC 3 cut(s) 398, 496, 838
PleI GAGTC 1 cut(s) 31
PpsI GAGTC 1 cut(s) 31
PshAI GACNNNNGTC 1 cut(s) 140
PshBI ATTAAT 1 cut(s) 591
Psp124BI GAGCTC 1 cut(s) 339
Psp6I CCWGG 1 cut(s) 239
PspFI CCCAGC 1 cut(s) 970
PspGI CCWGG 1 cut(s) 239
PspN4I GGNNCC 4 cut(s) 245, 308, 318, 903
PspPI GGNCC 2 cut(s) 317, 901
PsuI RGATCY 1 cut(s) 572
PvuII CAGCTG 1 cut(s) 970
RruI TCGCGA 1 cut(s) 871
RsaI GTAC 2 cut(s) 164, 948
RsaNI GTAC 2 cut(s) 163, 947
SacI GAGCTC 1 cut(s) 339
SalI GTCGAC 1 cut(s) 113
SaqAI TTAA 4 cut(s) 186, 438, 591, 851
Sau3AI GATC 5 cut(s) 150, 433, 518, 572, 766
Sau96I GGNCC 2 cut(s) 317, 901
SchI GAGTC 1 cut(s) 32
ScrFI CCNGG 1 cut(s) 241
SduI GDGCHC 3 cut(s) 311, 327, 339
SfaNI GCATC 1 cut(s) 502
SfcI CTRYAG 1 cut(s) 732
SfuI TTCGAA 1 cut(s) 401
SinI GGWCC 1 cut(s) 317
SmlI CTYRAG 2 cut(s) 168, 421
SmoI CTYRAG 2 cut(s) 168, 421
Sse9I AATT 6 cut(s) 28, 292, 371, 522, 692, 978
SspMI CTAG 1 cut(s) 408
SstI GAGCTC 1 cut(s) 339
StyD4I CCNGG 1 cut(s) 239
StyI CCWWGG 2 cut(s) 118, 312
TaiI ACGT 3 cut(s) 162, 177, 952
TaqI TCGA 5 cut(s) 114, 396, 401, 494, 990
TasI AATT 6 cut(s) 28, 292, 371, 522, 692, 978
TatI WGTACW 1 cut(s) 162
TfiI GAWTC 3 cut(s) 398, 496, 838
Tru1I TTAA 4 cut(s) 186, 438, 591, 851
Tru9I TTAA 4 cut(s) 186, 438, 591, 851
TscAI CASTG 3 cut(s) 332, 544, 919
TspDTI ATGAA 5 cut(s) 90, 485, 577, 638, 830
TspRI CASTG 3 cut(s) 332, 544, 919
VpaK11BI GGWCC 1 cut(s) 317
VspI ATTAAT 1 cut(s) 591
XcmI CCANNNNNNNNNTGG 1 cut(s) 100
XmiI GTMKAC 1 cut(s) 114
XspI CTAG 1 cut(s) 408
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.