RchiOBHm_Chr3g0470851

Bifunctional monodehydroascorbate reductase and carbonic anhydrase nectarin-3-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
3
OG0001343
Physical Location & Seq
Reverse (-)
16785458 .. 16786810
1353 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ43661

Sequence Viewer

Length: 894 bp
ATGCTGTATCTATTATTCATTGCAGACGTAGTGATGGTTAAATCAAAGAAAGTCCATCTTCAGTTCTTTGCTTTCACTCTTTTTCTTCTGTCCTGGCACCCCATCAGACCCGTCAGTGGCCAAGGAGTGGAAGAAATAGGGTATGATTATAGGCAAGGGAGCCCCAAGGGACCAGAGCACTGGGGAGAGCTCAAGGAAGAATGGAAGGAATGCAAAGATGGAAAATTACAATCGCCAATAGATTTGTTGGATTCGAATGCTAGAAAAGCAATCTCAAGAGTAGATGATCTTAACATGAGCTATAAGCCTTCAAATGCAACCATGAAGCATGAAGGTTATGCTATTTCGATTCAATGGGAGGGTGATGCTGGATCAATTCAAATAAATGGCACTGATTACTTGCTCAAAAGATGTCATTGGCACAGATCTTCTGAACATTTCATTAATGGCAGAAGGTTTCTCTTTCTCTTTGCTTCTCTGTTTGAAAAATCCAAAATACTTCATATATGTCTAAACAGAGTTCTTTTTATCAGTTATGATTTGGAATTGCACATGGTTCACCAAAGCCAGAACAACGATGTGGCTGTAGTCGCATTCCTATACCAAAGTGGCAATCCCGATCCTTTCCTGTCAAAGATAAGCAACGATGTATCCTCCTTAAGTGGTACAAAGGAGGCACGCTTGGGAGTGATTGATCCAAGTTTAATAAAATGGCCAAGCTCACGATTTTACAGATACATGGGCTCACTCACGACCCCTCCTTGCACTGGAGGAGTTATTTGGAGCGTATACGAAGAGGTACATTCTGTTTCAGAAGAACAGCTGGGGCTAATTGAACAAGTCGATGTAAGTAACATATTTTTATTGACTGACCTCATCTACACATCAAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00910 map00910
Pfam Domains
Protein Families
Carb_anhydrase

Protein Analysis

297

Amino Acids

33.91

Weight (kDa)

6.26

Isoelectric Point (pI)

47.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Carb_anhydrase PF00194 56 - 281 2.7e-27 Eukaryotic-type carbonic anhydrase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 96
AccB7I CCANNNNNTGG 1 cut(s) 127
AccI GTMKAC 1 cut(s) 789
AclWI GGATC 3 cut(s) 379, 614, 689
AcoI YGGCCR 2 cut(s) 118, 713
AcuI CTGAAG 1 cut(s) 44
AfaI GTAC 2 cut(s) 667, 801
AfiI CCNNNNNNNGG 3 cut(s) 116, 127, 767
AflII CTTAAG 1 cut(s) 658
AgsI TTSAA 5 cut(s) 312, 353, 380, 485, 836
AjnI CCWGG 1 cut(s) 92
AluBI AGCT 4 cut(s) 190, 300, 720, 823
AluI AGCT 4 cut(s) 190, 300, 720, 823
Alw21I GWGCWC 2 cut(s) 180, 192
AlwI GGATC 3 cut(s) 379, 614, 689
AoxI GGCC 2 cut(s) 118, 713
AseI ATTAAT 1 cut(s) 444
AspS9I GGNCC 1 cut(s) 170
AsuHPI GGTGA 2 cut(s) 374, 551
AsuII TTCGAA 1 cut(s) 254
AvaII GGWCC 1 cut(s) 170
BalI TGGCCA 2 cut(s) 120, 715
BanI GGYRCC 1 cut(s) 96
BanII GRGCYC 3 cut(s) 164, 192, 746
Bbv12I GWGCWC 2 cut(s) 180, 192
BccI CCATC 4 cut(s) 28, 63, 110, 212
BciT130I CCWGG 1 cut(s) 94
BciVI GTATCC 1 cut(s) 661
BfaI CTAG 1 cut(s) 261
BfmI CTRYAG 1 cut(s) 585
BfrI CTTAAG 1 cut(s) 658
BfuI GTATCC 1 cut(s) 661
BglII AGATCT 1 cut(s) 425
Bme1390I CCNGG 1 cut(s) 94
Bme18I GGWCC 1 cut(s) 170
BmgT120I GGNCC 1 cut(s) 170
BmiI GGNNCC 3 cut(s) 98, 161, 171
BmrFI CCNGG 1 cut(s) 94
BmrI ACTGGG 1 cut(s) 190
BmsI GCATC 1 cut(s) 355
BmuI ACTGGG 1 cut(s) 190
BpmI CTGGAG 1 cut(s) 789
Bpu14I TTCGAA 1 cut(s) 254
BpuEI CTTGAG 2 cut(s) 176, 259
BsaJI CCNNGG 2 cut(s) 121, 165
BsaXI ACNNNNNCTCC 2 cut(s) 742, 772
Bsc4I CCNNNNNNNGG 3 cut(s) 116, 127, 767
Bse1I ACTGG 2 cut(s) 185, 772
Bse3DI GCAATG 1 cut(s) 18
BseBI CCWGG 1 cut(s) 94
BseDI CCNNGG 2 cut(s) 121, 165
BseLI CCNNNNNNNGG 3 cut(s) 116, 127, 767
BseMI GCAATG 1 cut(s) 18
BseNI ACTGG 2 cut(s) 185, 772
BseRI GAGGAG 1 cut(s) 786
BseYI CCCAGC 1 cut(s) 823
BshFI GGCC 2 cut(s) 120, 715
BshNI GGYRCC 1 cut(s) 96
BsiHKAI GWGCWC 2 cut(s) 180, 192
BslFI GGGAC 1 cut(s) 183
BslI CCNNNNNNNGG 3 cut(s) 116, 127, 767
BsmFI GGGAC 1 cut(s) 183
BsmI GAATGC 3 cut(s) 215, 262, 593
BsnI GGCC 2 cut(s) 120, 715
Bsp119I TTCGAA 1 cut(s) 254
Bsp1286I GDGCHC 4 cut(s) 164, 180, 192, 746
Bsp143I GATC 5 cut(s) 286, 371, 425, 619, 694
BspANI GGCC 2 cut(s) 120, 715
BspLI GGNNCC 3 cut(s) 98, 161, 171
BspPI GGATC 3 cut(s) 379, 614, 689
BspT104I TTCGAA 1 cut(s) 254
BspT107I GGYRCC 1 cut(s) 96
BspTI CTTAAG 1 cut(s) 658
BsrDI GCAATG 1 cut(s) 18
BsrI ACTGG 2 cut(s) 185, 772
BssECI CCNNGG 2 cut(s) 121, 165
BssMI GATC 5 cut(s) 286, 371, 425, 619, 694
BssNAI GTATAC 1 cut(s) 790
BssT1I CCWWGG 2 cut(s) 121, 165
Bst1107I GTATAC 1 cut(s) 790
Bst2UI CCWGG 1 cut(s) 94
Bst6I CTCTTC 1 cut(s) 789
BstAFI CTTAAG 1 cut(s) 658
BstBI TTCGAA 1 cut(s) 254
BstC8I GCNNGC 1 cut(s) 679
BstKTI GATC 5 cut(s) 289, 374, 428, 622, 697
BstMBI GATC 5 cut(s) 286, 371, 425, 619, 694
BstMWI GCNNNNNNNGC 2 cut(s) 266, 590
BstNI CCWGG 1 cut(s) 94
BstSCI CCNGG 1 cut(s) 92
BstSFI CTRYAG 1 cut(s) 585
BstX2I RGATCY 1 cut(s) 425
BstXI CCANNNNNNTGG 1 cut(s) 180
BstYI RGATCY 1 cut(s) 425
BstZ17I GTATAC 1 cut(s) 790
BsuI GTATCC 1 cut(s) 661
BsuRI GGCC 2 cut(s) 120, 715
BtsIMutI CAGTG 4 cut(s) 121, 178, 390, 765
Cac8I GCNNGC 1 cut(s) 679
Cfr13I GGNCC 1 cut(s) 170
Csp6I GTAC 2 cut(s) 666, 800
CviAII CATG 5 cut(s) 295, 322, 329, 553, 739
CviQI GTAC 2 cut(s) 666, 800
DpnI GATC 5 cut(s) 288, 373, 427, 621, 696
DpnII GATC 5 cut(s) 286, 371, 425, 619, 694
EaeI YGGCCR 2 cut(s) 118, 713
Eam1104I CTCTTC 1 cut(s) 789
EarI CTCTTC 1 cut(s) 789
Ecl136II GAGCTC 1 cut(s) 190
Eco130I CCWWGG 2 cut(s) 121, 165
Eco24I GRGCYC 3 cut(s) 164, 192, 746
Eco47I GGWCC 1 cut(s) 170
Eco53kI GAGCTC 1 cut(s) 190
Eco57I CTGAAG 1 cut(s) 44
EcoICRI GAGCTC 1 cut(s) 190
EcoRII CCWGG 1 cut(s) 92
EcoT14I CCWWGG 2 cut(s) 121, 165
EcoT38I GRGCYC 3 cut(s) 164, 192, 746
ErhI CCWWGG 2 cut(s) 121, 165
FaeI CATG 5 cut(s) 298, 325, 332, 556, 742
FalI AAGNNNNNCTT 2 cut(s) 42, 74
FaqI GGGAC 1 cut(s) 183
FatI CATG 5 cut(s) 294, 321, 328, 552, 738
FblI GTMKAC 1 cut(s) 789
FriOI GRGCYC 3 cut(s) 164, 192, 746
FspBI CTAG 1 cut(s) 261
GsaI CCCAGC 1 cut(s) 827
GsuI CTGGAG 1 cut(s) 789
HaeIII GGCC 2 cut(s) 120, 715
Hin1II CATG 5 cut(s) 298, 325, 332, 556, 742
HinfI GANTC 2 cut(s) 251, 349
HphI GGTGA 2 cut(s) 374, 551
Hpy166II GTNNAC 2 cut(s) 559, 790
Hpy188I TCNGA 3 cut(s) 107, 433, 814
Hpy188III TCNNGA 4 cut(s) 276, 617, 723, 751
Hpy8I GTNNAC 2 cut(s) 559, 790
HpyAV CCTTC 4 cut(s) 199, 318, 326, 447
HpyCH4IV ACGT 1 cut(s) 27
HpyCH4V TGCA 5 cut(s) 23, 213, 317, 550, 765
HpyF10VI GCNNNNNNNGC 2 cut(s) 266, 590
HpySE526I ACGT 1 cut(s) 27
Hsp92II CATG 5 cut(s) 298, 325, 332, 556, 742
Kzo9I GATC 5 cut(s) 286, 371, 425, 619, 694
LmnI GCTCC 2 cut(s) 159, 783
LpnPI CCDG 9 cut(s) 79, 106, 166, 186, 354, 581, 641, 753, 809
LweI GCATC 1 cut(s) 355
MaeI CTAG 1 cut(s) 261
MaeII ACGT 1 cut(s) 27
MaeIII GTNAC 1 cut(s) 851
MalI GATC 5 cut(s) 288, 373, 427, 621, 696
MboI GATC 5 cut(s) 286, 371, 425, 619, 694
MboII GAAGA 7 cut(s) 50, 77, 143, 209, 420, 806, 827
MflI RGATCY 1 cut(s) 425
MhlI GDGCHC 4 cut(s) 164, 180, 192, 746
MlsI TGGCCA 2 cut(s) 120, 715
MluCI AATT 4 cut(s) 224, 375, 545, 831
MluNI TGGCCA 2 cut(s) 120, 715
MmeI TCCRAC 1 cut(s) 228
MnlI CCTC 7 cut(s) 352, 664, 667, 764, 768, 790, 884
Mox20I TGGCCA 2 cut(s) 120, 715
MscI TGGCCA 2 cut(s) 120, 715
MseI TTAA 5 cut(s) 39, 291, 444, 659, 704
Msp20I TGGCCA 2 cut(s) 120, 715
MspA1I CMGCKG 1 cut(s) 823
MspCI CTTAAG 1 cut(s) 658
MspR9I CCNGG 1 cut(s) 94
Mva1269I GAATGC 3 cut(s) 215, 262, 593
MvaI CCWGG 1 cut(s) 94
MwoI GCNNNNNNNGC 2 cut(s) 266, 590
NdeII GATC 5 cut(s) 286, 371, 425, 619, 694
NlaIII CATG 5 cut(s) 298, 325, 332, 556, 742
NlaIV GGNNCC 3 cut(s) 98, 161, 171
NspV TTCGAA 1 cut(s) 254
PctI GAATGC 3 cut(s) 215, 262, 593
PfeI GAWTC 2 cut(s) 251, 349
PflMI CCANNNNNTGG 1 cut(s) 127
PshBI ATTAAT 1 cut(s) 444
Psp124BI GAGCTC 1 cut(s) 192
Psp6I CCWGG 1 cut(s) 92
PspFI CCCAGC 1 cut(s) 823
PspGI CCWGG 1 cut(s) 92
PspN4I GGNNCC 3 cut(s) 98, 161, 171
PspPI GGNCC 1 cut(s) 170
PsuI RGATCY 1 cut(s) 425
PvuII CAGCTG 1 cut(s) 823
RsaI GTAC 2 cut(s) 667, 801
RsaNI GTAC 2 cut(s) 666, 800
SacI GAGCTC 1 cut(s) 192
SaqAI TTAA 5 cut(s) 39, 291, 444, 659, 704
Sau3AI GATC 5 cut(s) 286, 371, 425, 619, 694
Sau96I GGNCC 1 cut(s) 170
ScrFI CCNGG 1 cut(s) 94
SduI GDGCHC 4 cut(s) 164, 180, 192, 746
SetI ASST 9 cut(s) 30, 192, 302, 337, 458, 722, 801, 825, 876
SfaNI GCATC 1 cut(s) 355
SfcI CTRYAG 1 cut(s) 585
SfuI TTCGAA 1 cut(s) 254
SinI GGWCC 1 cut(s) 170
SmlI CTYRAG 3 cut(s) 191, 274, 658
SmoI CTYRAG 3 cut(s) 191, 274, 658
Sse9I AATT 4 cut(s) 224, 375, 545, 831
SspMI CTAG 1 cut(s) 261
SstI GAGCTC 1 cut(s) 192
StyD4I CCNGG 1 cut(s) 92
StyI CCWWGG 2 cut(s) 121, 165
TaiI ACGT 1 cut(s) 30
TaqI TCGA 3 cut(s) 254, 347, 843
TasI AATT 4 cut(s) 224, 375, 545, 831
TfiI GAWTC 2 cut(s) 251, 349
Tru1I TTAA 5 cut(s) 39, 291, 444, 659, 704
Tru9I TTAA 5 cut(s) 39, 291, 444, 659, 704
TscAI CASTG 4 cut(s) 121, 185, 397, 772
TspDTI ATGAA 5 cut(s) 7, 338, 345, 430, 491
TspRI CASTG 4 cut(s) 121, 185, 397, 772
Van91I CCANNNNNTGG 1 cut(s) 127
Vha464I CTTAAG 1 cut(s) 658
VpaK11BI GGWCC 1 cut(s) 170
VspI ATTAAT 1 cut(s) 444
XmiI GTMKAC 1 cut(s) 789
XspI CTAG 1 cut(s) 261
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.