Rh3DG187800

Bifunctional monodehydroascorbate reductase and carbonic anhydrase nectarin-3-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3D
OG0001343
Physical Location & Seq
Forward (+)
16740915 .. 16742353
1439 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3DG187800.1

Sequence Viewer

Length: 816 bp
ATGGTTAAATCAAAGAAAGTCCATCTTCAGTTCTTTGCTTTCACTCTTTTTCTTCTGTCCTGGCACTCCATCAGACCCGTCAGAGGCCAAGGAGTGGAAGAAATAGGGTATGATTATAGGCAAGGGAGCCCCAAGGGACCAGAGTACTGGGGAGAGCTCAAGGAAGAATGGAAGGAATGCAAAGATGGAAAATTACAATCGCCAATAGATTTGTTGGATTCGAATGCTAGAAAAGCAATCTCAAGAGTAGATGATCTTAACATGTGCTATAAGCCCTCAAATGCAACGATGAAGCATGAAGGTTATGCTATTTCGATTCAATGGGAGGGTGATGCTGGATCAATTCAAATAAATGGCACTGAATACTTGCTCAAAAGATGTCATTGGCACAGATCCTCTGAACATTTCATTAATGGCAGAAGGTTTCTCTTTCTCTTTGCTTCTCTGTTTGTAAAATCCAAAATACTTCATATATGTCTAAACAGAGTTCTTTTTATCAGTTACGATTTGGAATTGCACATGGTTCACCAAAGCCAGAACAACGATGTGGCTGTAGTCGCATTCCTATACCAAAGTGGCAATCCCGATCCTTTCCTGTCAAAGATAAGCAACGATGTATCCTCCCTAAGTGGTACAAAGGAGGCACGCTTGGGAGTGATTCATCCAAGTTTAATAAAATGGCCAAGCTCACGATTTTATAGATATATAGGCTCACTCACGGCCCCTCCTTGCACTGAAGGAGTTATTTGGAGCGTAAGCGAAGAGGTACGTTCTGTTTCAAAAGAACAGCTGGGGCTAATTGAACAAGTCGATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00910 map00910
Pfam Domains
Protein Families
Carb_anhydrase

Protein Analysis

271

Amino Acids

31.0

Weight (kDa)

8.2

Isoelectric Point (pI)

49.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Carb_anhydrase PF00194 46 - 269 9.9e-26 Eukaryotic-type carbonic anhydrase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 94
AclWI GGATC 3 cut(s) 346, 387, 581
AcoI YGGCCR 1 cut(s) 680
AcuI CTGAAG 2 cut(s) 11, 756
AfaI GTAC 3 cut(s) 146, 634, 768
AfiI CCNNNNNNNGG 2 cut(s) 83, 94
AflIII ACRYGT 1 cut(s) 261
AgsI TTSAA 4 cut(s) 320, 347, 780, 803
AjnI CCWGG 1 cut(s) 59
AluBI AGCT 3 cut(s) 157, 687, 790
AluI AGCT 3 cut(s) 157, 687, 790
Alw21I GWGCWC 1 cut(s) 159
AlwI GGATC 3 cut(s) 346, 387, 581
AoxI GGCC 3 cut(s) 85, 680, 720
AseI ATTAAT 1 cut(s) 411
AspS9I GGNCC 2 cut(s) 137, 721
AsuHPI GGTGA 2 cut(s) 341, 518
AsuII TTCGAA 1 cut(s) 221
AvaII GGWCC 1 cut(s) 137
BalI TGGCCA 1 cut(s) 682
BanII GRGCYC 2 cut(s) 131, 159
Bbv12I GWGCWC 1 cut(s) 159
BccI CCATC 3 cut(s) 30, 77, 179
BceAI ACGGC 1 cut(s) 735
BciT130I CCWGG 1 cut(s) 61
BciVI GTATCC 1 cut(s) 628
BfaI CTAG 1 cut(s) 228
BfmI CTRYAG 1 cut(s) 552
BfuI GTATCC 1 cut(s) 628
BmcAI AGTACT 1 cut(s) 146
Bme1390I CCNGG 1 cut(s) 61
Bme18I GGWCC 1 cut(s) 137
BmgT120I GGNCC 2 cut(s) 137, 721
BmiI GGNNCC 3 cut(s) 128, 138, 723
BmrFI CCNGG 1 cut(s) 61
BmrI ACTGGG 1 cut(s) 157
BmsI GCATC 1 cut(s) 322
BmuI ACTGGG 1 cut(s) 157
Bpu14I TTCGAA 1 cut(s) 221
BpuEI CTTGAG 2 cut(s) 143, 226
BsaJI CCNNGG 2 cut(s) 88, 132
BsaXI ACNNNNNCTCC 2 cut(s) 709, 739
Bsc4I CCNNNNNNNGG 2 cut(s) 83, 94
Bse1I ACTGG 1 cut(s) 152
BseBI CCWGG 1 cut(s) 61
BseDI CCNNGG 2 cut(s) 88, 132
BseGI GGATG 1 cut(s) 661
BseLI CCNNNNNNNGG 2 cut(s) 83, 94
BseNI ACTGG 1 cut(s) 152
BseYI CCCAGC 1 cut(s) 790
BshFI GGCC 3 cut(s) 87, 682, 722
BsiHKAI GWGCWC 1 cut(s) 159
BslFI GGGAC 1 cut(s) 150
BslI CCNNNNNNNGG 2 cut(s) 83, 94
BsmFI GGGAC 1 cut(s) 150
BsmI GAATGC 3 cut(s) 182, 229, 560
BsnI GGCC 3 cut(s) 87, 682, 722
Bsp119I TTCGAA 1 cut(s) 221
Bsp1286I GDGCHC 2 cut(s) 131, 159
Bsp143I GATC 4 cut(s) 253, 338, 392, 586
BspANI GGCC 3 cut(s) 87, 682, 722
BspLI GGNNCC 3 cut(s) 128, 138, 723
BspPI GGATC 3 cut(s) 346, 387, 581
BspT104I TTCGAA 1 cut(s) 221
BsrI ACTGG 1 cut(s) 152
BssECI CCNNGG 2 cut(s) 88, 132
BssMI GATC 4 cut(s) 253, 338, 392, 586
BssT1I CCWWGG 2 cut(s) 88, 132
Bst2UI CCWGG 1 cut(s) 61
Bst6I CTCTTC 1 cut(s) 756
BstBI TTCGAA 1 cut(s) 221
BstC8I GCNNGC 1 cut(s) 646
BstDEI CTNAG 1 cut(s) 626
BstF5I GGATG 1 cut(s) 661
BstKTI GATC 4 cut(s) 256, 341, 395, 589
BstMBI GATC 4 cut(s) 253, 338, 392, 586
BstMWI GCNNNNNNNGC 2 cut(s) 233, 557
BstNI CCWGG 1 cut(s) 61
BstNSI RCATGY 1 cut(s) 265
BstSCI CCNGG 1 cut(s) 59
BstSFI CTRYAG 1 cut(s) 552
BstX2I RGATCY 1 cut(s) 392
BstXI CCANNNNNNTGG 1 cut(s) 147
BstYI RGATCY 1 cut(s) 392
BsuI GTATCC 1 cut(s) 628
BsuRI GGCC 3 cut(s) 87, 682, 722
BtsCI GGATG 1 cut(s) 661
BtsIMutI CAGTG 2 cut(s) 357, 732
Cac8I GCNNGC 1 cut(s) 646
Cfr13I GGNCC 2 cut(s) 137, 721
Csp6I GTAC 3 cut(s) 145, 633, 767
CviAII CATG 3 cut(s) 262, 296, 520
CviQI GTAC 3 cut(s) 145, 633, 767
DdeI CTNAG 1 cut(s) 626
DpnI GATC 4 cut(s) 255, 340, 394, 588
DpnII GATC 4 cut(s) 253, 338, 392, 586
EaeI YGGCCR 1 cut(s) 680
Eam1104I CTCTTC 1 cut(s) 756
EarI CTCTTC 1 cut(s) 756
Ecl136II GAGCTC 1 cut(s) 157
Eco130I CCWWGG 2 cut(s) 88, 132
Eco24I GRGCYC 2 cut(s) 131, 159
Eco47I GGWCC 1 cut(s) 137
Eco53kI GAGCTC 1 cut(s) 157
Eco57I CTGAAG 2 cut(s) 11, 756
EcoICRI GAGCTC 1 cut(s) 157
EcoRII CCWGG 1 cut(s) 59
EcoT14I CCWWGG 2 cut(s) 88, 132
EcoT38I GRGCYC 2 cut(s) 131, 159
ErhI CCWWGG 2 cut(s) 88, 132
FaeI CATG 3 cut(s) 265, 299, 523
FalI AAGNNNNNCTT 2 cut(s) 9, 41
FaqI GGGAC 1 cut(s) 150
FatI CATG 3 cut(s) 261, 295, 519
FokI GGATG 1 cut(s) 648
FriOI GRGCYC 2 cut(s) 131, 159
FspBI CTAG 1 cut(s) 228
GsaI CCCAGC 1 cut(s) 794
HaeIII GGCC 3 cut(s) 87, 682, 722
Hin1II CATG 3 cut(s) 265, 299, 523
HinfI GANTC 3 cut(s) 218, 316, 658
HphI GGTGA 2 cut(s) 341, 518
Hpy166II GTNNAC 1 cut(s) 526
Hpy188I TCNGA 3 cut(s) 74, 83, 400
Hpy188III TCNNGA 3 cut(s) 243, 584, 690
Hpy8I GTNNAC 1 cut(s) 526
HpyAV CCTTC 4 cut(s) 166, 293, 414, 731
HpyCH4IV ACGT 1 cut(s) 769
HpyCH4V TGCA 4 cut(s) 180, 284, 517, 732
HpyF10VI GCNNNNNNNGC 2 cut(s) 233, 557
HpyF3I CTNAG 1 cut(s) 626
HpySE526I ACGT 1 cut(s) 769
Hsp92II CATG 3 cut(s) 265, 299, 523
Kzo9I GATC 4 cut(s) 253, 338, 392, 586
LmnI GCTCC 2 cut(s) 126, 750
LpnPI CCDG 8 cut(s) 46, 73, 133, 153, 321, 548, 608, 776
LweI GCATC 1 cut(s) 322
MaeI CTAG 1 cut(s) 228
MaeII ACGT 1 cut(s) 769
MaeIII GTNAC 1 cut(s) 500
MalI GATC 4 cut(s) 255, 340, 394, 588
MboI GATC 4 cut(s) 253, 338, 392, 586
MboII GAAGA 5 cut(s) 17, 44, 110, 176, 773
MflI RGATCY 1 cut(s) 392
MhlI GDGCHC 2 cut(s) 131, 159
MlsI TGGCCA 1 cut(s) 682
MluCI AATT 4 cut(s) 191, 342, 512, 798
MluNI TGGCCA 1 cut(s) 682
MmeI TCCRAC 1 cut(s) 195
MnlI CCTC 8 cut(s) 77, 286, 319, 406, 631, 634, 735, 757
Mox20I TGGCCA 1 cut(s) 682
MscI TGGCCA 1 cut(s) 682
MseI TTAA 4 cut(s) 6, 258, 411, 671
Msp20I TGGCCA 1 cut(s) 682
MspA1I CMGCKG 1 cut(s) 790
MspR9I CCNGG 1 cut(s) 61
Mva1269I GAATGC 3 cut(s) 182, 229, 560
MvaI CCWGG 1 cut(s) 61
MwoI GCNNNNNNNGC 2 cut(s) 233, 557
NdeII GATC 4 cut(s) 253, 338, 392, 586
NlaIII CATG 3 cut(s) 265, 299, 523
NlaIV GGNNCC 3 cut(s) 128, 138, 723
NspI RCATGY 1 cut(s) 265
NspV TTCGAA 1 cut(s) 221
PciI ACATGT 1 cut(s) 261
PctI GAATGC 3 cut(s) 182, 229, 560
PfeI GAWTC 3 cut(s) 218, 316, 658
PflMI CCANNNNNTGG 1 cut(s) 94
PscI ACATGT 1 cut(s) 261
PshBI ATTAAT 1 cut(s) 411
Psp124BI GAGCTC 1 cut(s) 159
Psp6I CCWGG 1 cut(s) 59
PspFI CCCAGC 1 cut(s) 790
PspGI CCWGG 1 cut(s) 59
PspN4I GGNNCC 3 cut(s) 128, 138, 723
PspPI GGNCC 2 cut(s) 137, 721
PsuI RGATCY 1 cut(s) 392
PvuII CAGCTG 1 cut(s) 790
RsaI GTAC 3 cut(s) 146, 634, 768
RsaNI GTAC 3 cut(s) 145, 633, 767
SacI GAGCTC 1 cut(s) 159
SaqAI TTAA 4 cut(s) 6, 258, 411, 671
Sau3AI GATC 4 cut(s) 253, 338, 392, 586
Sau96I GGNCC 2 cut(s) 137, 721
ScaI AGTACT 1 cut(s) 146
ScrFI CCNGG 1 cut(s) 61
SduI GDGCHC 2 cut(s) 131, 159
SetI ASST 7 cut(s) 159, 304, 425, 689, 768, 772, 792
SfaNI GCATC 1 cut(s) 322
SfcI CTRYAG 1 cut(s) 552
SfuI TTCGAA 1 cut(s) 221
SinI GGWCC 1 cut(s) 137
SmlI CTYRAG 2 cut(s) 158, 241
SmoI CTYRAG 2 cut(s) 158, 241
Sse9I AATT 4 cut(s) 191, 342, 512, 798
SspMI CTAG 1 cut(s) 228
SstI GAGCTC 1 cut(s) 159
StyD4I CCNGG 1 cut(s) 59
StyI CCWWGG 2 cut(s) 88, 132
TaiI ACGT 1 cut(s) 772
TaqI TCGA 3 cut(s) 221, 314, 810
TasI AATT 4 cut(s) 191, 342, 512, 798
TatI WGTACW 1 cut(s) 144
TfiI GAWTC 3 cut(s) 218, 316, 658
Tru1I TTAA 4 cut(s) 6, 258, 411, 671
Tru9I TTAA 4 cut(s) 6, 258, 411, 671
TscAI CASTG 2 cut(s) 364, 739
TspDTI ATGAA 5 cut(s) 305, 312, 397, 458, 650
TspRI CASTG 2 cut(s) 364, 739
Van91I CCANNNNNTGG 1 cut(s) 94
VpaK11BI GGWCC 1 cut(s) 137
VspI ATTAAT 1 cut(s) 411
XceI RCATGY 1 cut(s) 265
XspI CTAG 1 cut(s) 228
ZrmI AGTACT 1 cut(s) 146
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.