Rorug03G0113200

Bifunctional monodehydroascorbate reductase and carbonic anhydrase nectarin-3-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0001343
Physical Location & Seq
Reverse (-)
9153327 .. 9155536
2210 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0113200.1

Sequence Viewer

Length: 942 bp
ATGGAGAAAAACCAGAGCGATTCCCCTAACGCCGTTAAGAAGCCCATCCCGCCGTACGTCAAGGCCCTATCGGGCTCGCTGGGCGGGATTGTGGAGGCCTCGTGTCTCCAGCCCATTGACGTCATCAAGACGCGGCTGCAGCTCGACCGGACCGGGGGGTACAGGGGAATTATCCACTGCGGCACCACGGTTGCGCGCACGGAAGGCGTCCGGGCTTTGTGGAAGGGATTGACTCCTTTCGCGACGCATCTTACTTTGAAGTACGCGCTGAGGATGGGATCCAATGCGATTCTGCAGGGGGCGTTTAAGGACGCCCAGACCGGGAAGGTCAGCAATCACGGCCGGTTCATTTCCGGGTTCGGTGCCGGAGTTCTCGAGGCTCTTGTTATTGTCACTCCTTTCGAGGTAGTGAAAATTAGACTGCAGCAACAGAGAGGATTGAGTCATGAACTTCTGAAGTATAAGGGTCCTGTACACTGTGCTCGTAGGATCATCCGGGAGGAAGGTCTTCGTGGGCTCTGGTCGGGGGCTTCTCCAACTGTTATGCGCAATGGGACAAACCAGGCGGCTATGTTTTCAGCCAAAAATGCTTTTGATGTTCTCCTATGGAAGAAACATGAAGGAGATGGGAGAGTCCTGCTGCCATGGCAGTCTATGACATCAGGTTTCCTCGCAGGAACAGCAGGTCCCATTTGCACCGGTCCCTTTGATGTTGTCAAAACAAGGCTCATGGCTCAGGGCAGAGGGGTTGGTGAGTTGAAATACAAAGGCCTTTTCCATGCCATCAGGACAATATATGCAGAGGAAGGGCTTCTTGCCTTGTGGAAAGGACTGCTGCCGCGGCTCATGAGGATACCACCCGGGCAAGCCATCATGTGGGGTGTGGCTGACCAATTAATAGGATTGTATGAGAAACAATATCTTCCCCTTGCCCCCTTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00910 map00910
Pfam Domains
Protein Families
Carb_anhydrase

Protein Analysis

313

Amino Acids

34.15

Weight (kDa)

10.01

Isoelectric Point (pI)

31.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Mito_carr PF00153 16 - 102 1.7e-17 Mitochondrial carrier protein
Mito_carr PF00153 111 - 205 9.1e-21 Mitochondrial carrier protein
Mito_carr PF00153 215 - 304 3.4e-23 Mitochondrial carrier protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 123
Acc16I TGCGCA 1 cut(s) 548
Acc36I ACCTGC 1 cut(s) 674
AccB1I GGYRCC 2 cut(s) 182, 362
AccB7I CCANNNNNTGG 1 cut(s) 876
AccII CGCG 5 cut(s) 133, 196, 242, 266, 841
AciI CCGC 7 cut(s) 50, 84, 133, 180, 566, 839, 841
AclWI GGATC 3 cut(s) 273, 286, 497
AcoI YGGCCR 1 cut(s) 340
AcuI CTGAAG 1 cut(s) 476
AcyI GRCGYC 3 cut(s) 120, 207, 312
AfaI GTAC 4 cut(s) 56, 161, 263, 474
AfiI CCNNNNNNNGG 3 cut(s) 154, 321, 876
AgeI ACCGGT 1 cut(s) 698
AgsI TTSAA 2 cut(s) 259, 760
AjnI CCWGG 1 cut(s) 561
AloI GAACNNNNNNTCC 2 cut(s) 670, 702
AluBI AGCT 1 cut(s) 142
AluI AGCT 1 cut(s) 142
Alw21I GWGCWC 1 cut(s) 484
Alw26I GTCTC 1 cut(s) 110
AlwI GGATC 3 cut(s) 273, 286, 497
Ama87I CYCGRG 2 cut(s) 374, 860
AoxI GGCC 4 cut(s) 63, 96, 340, 769
ApeKI GCWGC 5 cut(s) 136, 139, 424, 640, 835
AseI ATTAAT 1 cut(s) 896
AsiGI ACCGGT 1 cut(s) 698
Asp700I GAANNNNTTC 2 cut(s) 507, 810
AspLEI GCGC 4 cut(s) 196, 198, 268, 549
AspS9I GGNCC 5 cut(s) 64, 150, 467, 686, 701
AsuC2I CCSGG 7 cut(s) 154, 212, 322, 355, 497, 861, 862
AsuHPI GGTGA 1 cut(s) 764
AvaI CYCGRG 2 cut(s) 374, 860
AvaII GGWCC 4 cut(s) 150, 467, 686, 701
BamHI GGATCC 1 cut(s) 278
BanI GGYRCC 2 cut(s) 182, 362
BanII GRGCYC 2 cut(s) 77, 519
BauI CACGAG 1 cut(s) 100
BbsI GAAGAC 1 cut(s) 500
Bbv12I GWGCWC 1 cut(s) 484
BbvCI CCTCAGC 1 cut(s) 269
BbvI GCAGC 5 cut(s) 123, 151, 436, 627, 822
BccI CCATC 5 cut(s) 53, 268, 620, 791, 878
BceAI ACGGC 3 cut(s) 17, 37, 355
BciT130I CCWGG 1 cut(s) 563
BciVI GTATCC 1 cut(s) 846
BcnI CCSGG 7 cut(s) 154, 212, 322, 355, 497, 861, 862
BcoDI GTCTC 1 cut(s) 110
BfmI CTRYAG 3 cut(s) 137, 293, 422
BfuAI ACCTGC 1 cut(s) 674
BfuI GTATCC 1 cut(s) 846
Bme1390I CCNGG 8 cut(s) 154, 212, 322, 355, 497, 563, 861, 862
Bme18I GGWCC 4 cut(s) 150, 467, 686, 701
BmeT110I CYCGRG 2 cut(s) 374, 860
BmgT120I GGNCC 5 cut(s) 64, 150, 467, 686, 701
BmiI GGNNCC 6 cut(s) 184, 280, 364, 468, 688, 703
BmrFI CCNGG 8 cut(s) 154, 212, 322, 355, 497, 563, 861, 862
BmsI GCATC 1 cut(s) 256
BpiI GAAGAC 1 cut(s) 500
BpmI CTGGAG 1 cut(s) 92
Bpu10I CCTNAGC 2 cut(s) 269, 735
BpuMI CCSGG 7 cut(s) 154, 212, 322, 355, 497, 861, 862
BsaHI GRCGYC 3 cut(s) 120, 207, 312
BsaJI CCNNGG 5 cut(s) 153, 186, 644, 839, 860
BsaWI WCCGGW 2 cut(s) 147, 698
BsaXI ACNNNNNCTCC 2 cut(s) 86, 116
Bsc4I CCNNNNNNNGG 3 cut(s) 154, 321, 876
Bse118I RCCGGY 2 cut(s) 342, 698
Bse3DI GCAATG 1 cut(s) 556
BseBI CCWGG 1 cut(s) 563
BseDI CCNNGG 5 cut(s) 153, 186, 644, 839, 860
BseGI GGATG 3 cut(s) 45, 279, 492
BseLI CCNNNNNNNGG 3 cut(s) 154, 321, 876
BseMI GCAATG 1 cut(s) 556
BseMII CTCAG 2 cut(s) 260, 749
BsePI GCGCGC 1 cut(s) 194
BseX3I CGGCCG 1 cut(s) 340
BseXI GCAGC 5 cut(s) 123, 151, 436, 627, 822
BseYI CCCAGC 1 cut(s) 79
Bsh1236I CGCG 5 cut(s) 133, 196, 242, 266, 841
Bsh1285I CGRYCG 2 cut(s) 148, 343
BshFI GGCC 4 cut(s) 65, 98, 342, 771
BshNI GGYRCC 2 cut(s) 182, 362
BshTI ACCGGT 1 cut(s) 698
BsiEI CGRYCG 2 cut(s) 148, 343
BsiHKAI GWGCWC 1 cut(s) 484
BsiHKCI CYCGRG 2 cut(s) 374, 860
BsiWI CGTACG 1 cut(s) 54
BslFI GGGAC 3 cut(s) 568, 672, 687
BslI CCNNNNNNNGG 3 cut(s) 154, 321, 876
BsmAI GTCTC 1 cut(s) 110
BsmFI GGGAC 3 cut(s) 568, 672, 687
BsnI GGCC 4 cut(s) 65, 98, 342, 771
BsoBI CYCGRG 2 cut(s) 374, 860
Bsp1286I GDGCHC 3 cut(s) 77, 484, 519
Bsp1407I TGTACA 1 cut(s) 472
Bsp143I GATC 2 cut(s) 278, 489
Bsp19I CCATGG 1 cut(s) 644
Bsp68I TCGCGA 1 cut(s) 242
BspACI CCGC 7 cut(s) 50, 84, 133, 180, 566, 839, 841
BspANI GGCC 4 cut(s) 65, 98, 342, 771
BspCNI CTCAG 2 cut(s) 261, 748
BspFNI CGCG 5 cut(s) 133, 196, 242, 266, 841
BspHI TCATGA 2 cut(s) 445, 846
BspLI GGNNCC 6 cut(s) 184, 280, 364, 468, 688, 703
BspMAI CTGCAG 3 cut(s) 141, 297, 426
BspMI ACCTGC 1 cut(s) 674
BspPI GGATC 3 cut(s) 273, 286, 497
BspT107I GGYRCC 2 cut(s) 182, 362
BsrDI GCAATG 1 cut(s) 556
BsrFI RCCGGY 2 cut(s) 342, 698
BsrGI TGTACA 1 cut(s) 472
BssAI RCCGGY 2 cut(s) 342, 698
BssECI CCNNGG 5 cut(s) 153, 186, 644, 839, 860
BssHII GCGCGC 1 cut(s) 194
BssMI GATC 2 cut(s) 278, 489
BssNI GRCGYC 3 cut(s) 120, 207, 312
BssSI CACGAG 1 cut(s) 100
BssT1I CCWWGG 1 cut(s) 644
Bst2BI CACGAG 1 cut(s) 100
Bst2UI CCWGG 1 cut(s) 563
Bst4CI ACNGT 3 cut(s) 190, 479, 541
BstACI GRCGYC 3 cut(s) 120, 207, 312
BstAUI TGTACA 1 cut(s) 472
BstC8I GCNNGC 3 cut(s) 77, 196, 867
BstDEI CTNAG 2 cut(s) 269, 735
BstDSI CCRYGG 3 cut(s) 186, 644, 839
BstF5I GGATG 3 cut(s) 45, 279, 492
BstFNI CGCG 5 cut(s) 133, 196, 242, 266, 841
BstHHI GCGC 4 cut(s) 196, 198, 268, 549
BstKTI GATC 2 cut(s) 281, 492
BstMAI GTCTC 1 cut(s) 110
BstMBI GATC 2 cut(s) 278, 489
BstMCI CGRYCG 2 cut(s) 148, 343
BstMWI GCNNNNNNNGC 9 cut(s) 49, 81, 139, 204, 339, 587, 646, 680, 841
BstNI CCWGG 1 cut(s) 563
BstSCI CCNGG 8 cut(s) 152, 210, 320, 353, 495, 561, 859, 860
BstSFI CTRYAG 3 cut(s) 137, 293, 422
BstUI CGCG 5 cut(s) 133, 196, 242, 266, 841
BstV1I GCAGC 5 cut(s) 123, 151, 436, 627, 822
BstV2I GAAGAC 1 cut(s) 500
BstX2I RGATCY 1 cut(s) 278
BstYI RGATCY 1 cut(s) 278
BstZI CGGCCG 1 cut(s) 340
BsuI GTATCC 1 cut(s) 846
BsuRI GGCC 4 cut(s) 65, 98, 342, 771
BtgI CCRYGG 3 cut(s) 186, 644, 839
BtsCI GGATG 3 cut(s) 45, 279, 492
BtsI GCAGTG 1 cut(s) 175
BtsIMutI CAGTG 2 cut(s) 175, 475
BtuMI TCGCGA 1 cut(s) 242
BveI ACCTGC 1 cut(s) 674
Cac8I GCNNGC 3 cut(s) 77, 196, 867
CciI TCATGA 2 cut(s) 445, 846
CfoI GCGC 4 cut(s) 196, 198, 268, 549
Cfr10I RCCGGY 2 cut(s) 342, 698
Cfr13I GGNCC 5 cut(s) 64, 150, 467, 686, 701
Cfr42I CCGCGG 1 cut(s) 842
Cfr9I CCCGGG 1 cut(s) 860
CpoI CGGWCCG 1 cut(s) 150
CseI GACGC 4 cut(s) 139, 196, 253, 320
Csp6I GTAC 4 cut(s) 55, 160, 262, 473
CspAI ACCGGT 1 cut(s) 698
CspI CGGWCCG 1 cut(s) 150
CviAII CATG 7 cut(s) 446, 617, 645, 730, 779, 847, 874
CviQI GTAC 4 cut(s) 55, 160, 262, 473
DdeI CTNAG 2 cut(s) 269, 735
DpnI GATC 2 cut(s) 280, 491
DpnII GATC 2 cut(s) 278, 489
EaeI YGGCCR 1 cut(s) 340
EagI CGGCCG 1 cut(s) 340
EclXI CGGCCG 1 cut(s) 340
Eco130I CCWWGG 1 cut(s) 644
Eco147I AGGCCT 2 cut(s) 98, 771
Eco24I GRGCYC 2 cut(s) 77, 519
Eco47I GGWCC 4 cut(s) 150, 467, 686, 701
Eco52I CGGCCG 1 cut(s) 340
Eco57I CTGAAG 1 cut(s) 476
Eco88I CYCGRG 2 cut(s) 374, 860
EcoO109I RGGNCCY 3 cut(s) 64, 467, 686
EcoRII CCWGG 1 cut(s) 561
EcoT14I CCWWGG 1 cut(s) 644
EcoT38I GRGCYC 2 cut(s) 77, 519
ErhI CCWWGG 1 cut(s) 644
FaeI CATG 7 cut(s) 449, 620, 648, 733, 782, 850, 877
FalI AAGNNNNNCTT 2 cut(s) 798, 830
FaqI GGGAC 3 cut(s) 568, 672, 687
FatI CATG 7 cut(s) 445, 616, 644, 729, 778, 846, 873
FauI CCCGC 2 cut(s) 57, 77
FokI GGATG 3 cut(s) 32, 286, 479
FriOI GRGCYC 2 cut(s) 77, 519
FspI TGCGCA 1 cut(s) 548
GlaI GCGC 4 cut(s) 195, 197, 267, 548
GsaI CCCAGC 1 cut(s) 83
GsuI CTGGAG 1 cut(s) 92
HaeIII GGCC 4 cut(s) 65, 98, 342, 771
HgaI GACGC 4 cut(s) 139, 196, 253, 320
HhaI GCGC 4 cut(s) 196, 198, 268, 549
Hin1I GRCGYC 3 cut(s) 120, 207, 312
Hin1II CATG 7 cut(s) 449, 620, 648, 733, 782, 850, 877
Hin6I GCGC 4 cut(s) 194, 196, 266, 547
HinP1I GCGC 4 cut(s) 194, 196, 266, 547
HinfI GANTC 5 cut(s) 20, 232, 289, 442, 633
HphI GGTGA 1 cut(s) 764
Hpy166II GTNNAC 1 cut(s) 475
Hpy188I TCNGA 1 cut(s) 456
Hpy188III TCNNGA 6 cut(s) 127, 241, 374, 446, 787, 847
Hpy8I GTNNAC 1 cut(s) 475
Hpy99I CGWCG 1 cut(s) 247
HpyAV CCTTC 6 cut(s) 197, 217, 319, 497, 614, 800
HpyCH4III ACNGT 3 cut(s) 190, 479, 541
HpyCH4IV ACGT 2 cut(s) 57, 120
HpyCH4V TGCA 5 cut(s) 139, 295, 424, 696, 800
HpyF10VI GCNNNNNNNGC 9 cut(s) 49, 81, 139, 204, 339, 587, 646, 680, 841
HpyF3I CTNAG 2 cut(s) 269, 735
HpySE526I ACGT 2 cut(s) 57, 120
Hsp92I GRCGYC 3 cut(s) 120, 207, 312
Hsp92II CATG 7 cut(s) 449, 620, 648, 733, 782, 850, 877
HspAI GCGC 4 cut(s) 194, 196, 266, 547
KspI CCGCGG 1 cut(s) 842
Kzo9I GATC 2 cut(s) 278, 489
Lsp1109I GCAGC 5 cut(s) 123, 151, 436, 627, 822
LweI GCATC 1 cut(s) 256
MaeII ACGT 2 cut(s) 57, 120
MaeIII GTNAC 1 cut(s) 391
MalI GATC 2 cut(s) 280, 491
MboI GATC 2 cut(s) 278, 489
MboII GAAGA 3 cut(s) 500, 622, 914
MflI RGATCY 1 cut(s) 278
MhlI GDGCHC 3 cut(s) 77, 484, 519
MluCI AATT 3 cut(s) 168, 414, 893
MlyI GAGTC 3 cut(s) 226, 451, 642
MmeI TCCRAC 1 cut(s) 560
MroXI GAANNNNTTC 2 cut(s) 507, 810
MseI TTAA 3 cut(s) 36, 306, 896
MspA1I CMGCKG 1 cut(s) 841
MspR9I CCNGG 8 cut(s) 154, 212, 322, 355, 497, 563, 861, 862
MvaI CCWGG 1 cut(s) 563
MvnI CGCG 5 cut(s) 133, 196, 242, 266, 841
MwoI GCNNNNNNNGC 9 cut(s) 49, 81, 139, 204, 339, 587, 646, 680, 841
NciI CCSGG 7 cut(s) 154, 212, 322, 355, 497, 861, 862
NcoI CCATGG 1 cut(s) 644
NdeII GATC 2 cut(s) 278, 489
NlaIII CATG 7 cut(s) 449, 620, 648, 733, 782, 850, 877
NlaIV GGNNCC 6 cut(s) 184, 280, 364, 468, 688, 703
NmuCI GTSAC 1 cut(s) 391
NruI TCGCGA 1 cut(s) 242
NsbI TGCGCA 1 cut(s) 548
PaeR7I CTCGAG 1 cut(s) 374
PagI TCATGA 2 cut(s) 445, 846
PauI GCGCGC 1 cut(s) 194
PceI AGGCCT 2 cut(s) 98, 771
PdmI GAANNNNTTC 2 cut(s) 507, 810
PfeI GAWTC 2 cut(s) 20, 289
Pfl23II CGTACG 1 cut(s) 54
PflMI CCANNNNNTGG 1 cut(s) 876
PfoI TCCNGGA 1 cut(s) 495
PinAI ACCGGT 1 cut(s) 698
PleI GAGTC 3 cut(s) 226, 450, 641
PpsI GAGTC 3 cut(s) 226, 450, 641
PpuMI RGGWCCY 2 cut(s) 467, 686
PshBI ATTAAT 1 cut(s) 896
Psp5II RGGWCCY 2 cut(s) 467, 686
Psp6I CCWGG 1 cut(s) 561
PspFI CCCAGC 1 cut(s) 79
PspGI CCWGG 1 cut(s) 561
PspLI CGTACG 1 cut(s) 54
PspN4I GGNNCC 6 cut(s) 184, 280, 364, 468, 688, 703
PspPI GGNCC 5 cut(s) 64, 150, 467, 686, 701
PspPPI RGGWCCY 2 cut(s) 467, 686
PstI CTGCAG 3 cut(s) 141, 297, 426
PsuI RGATCY 1 cut(s) 278
PteI GCGCGC 1 cut(s) 194
RruI TCGCGA 1 cut(s) 242
RsaI GTAC 4 cut(s) 56, 161, 263, 474
RsaNI GTAC 4 cut(s) 55, 160, 262, 473
Rsr2I CGGWCCG 1 cut(s) 150
RsrII CGGWCCG 1 cut(s) 150
SacII CCGCGG 1 cut(s) 842
SaqAI TTAA 3 cut(s) 36, 306, 896
Sau3AI GATC 2 cut(s) 278, 489
Sau96I GGNCC 5 cut(s) 64, 150, 467, 686, 701
SchI GAGTC 3 cut(s) 226, 451, 642
ScrFI CCNGG 8 cut(s) 154, 212, 322, 355, 497, 563, 861, 862
SduI GDGCHC 3 cut(s) 77, 484, 519
SetI ASST 8 cut(s) 60, 123, 144, 330, 408, 508, 667, 688
SfaNI GCATC 1 cut(s) 256
SfcI CTRYAG 3 cut(s) 137, 293, 422
Sfr274I CTCGAG 1 cut(s) 374
Sfr303I CCGCGG 1 cut(s) 842
SgrBI CCGCGG 1 cut(s) 842
SinI GGWCC 4 cut(s) 150, 467, 686, 701
SlaI CTCGAG 1 cut(s) 374
SmaI CCCGGG 1 cut(s) 862
SmlI CTYRAG 1 cut(s) 374
SmoI CTYRAG 1 cut(s) 374
Sse9I AATT 3 cut(s) 168, 414, 893
SseBI AGGCCT 2 cut(s) 98, 771
SsiI CCGC 7 cut(s) 50, 84, 133, 180, 566, 839, 841
StuI AGGCCT 2 cut(s) 98, 771
StyD4I CCNGG 8 cut(s) 152, 210, 320, 353, 495, 561, 859, 860
StyI CCWWGG 1 cut(s) 644
TaaI ACNGT 3 cut(s) 190, 479, 541
TaiI ACGT 2 cut(s) 60, 123
TaqI TCGA 3 cut(s) 144, 375, 402
TasI AATT 3 cut(s) 168, 414, 893
TatI WGTACW 1 cut(s) 472
TauI GCSGC 5 cut(s) 136, 183, 569, 841, 844
TfiI GAWTC 2 cut(s) 20, 289
Tru1I TTAA 3 cut(s) 36, 306, 896
Tru9I TTAA 3 cut(s) 36, 306, 896
TscAI CASTG 2 cut(s) 182, 482
TseFI GTSAC 1 cut(s) 391
TseI GCWGC 5 cut(s) 136, 139, 424, 640, 835
Tsp45I GTSAC 1 cut(s) 391
TspDTI ATGAA 3 cut(s) 337, 462, 633
TspGWI ACGGA 1 cut(s) 215
TspMI CCCGGG 1 cut(s) 860
TspRI CASTG 2 cut(s) 182, 482
Van91I CCANNNNNTGG 1 cut(s) 876
VpaK11BI GGWCC 4 cut(s) 150, 467, 686, 701
VspI ATTAAT 1 cut(s) 896
XhoI CTCGAG 1 cut(s) 374
XmaI CCCGGG 1 cut(s) 860
XmnI GAANNNNTTC 2 cut(s) 507, 810
ZraI GACGTC 1 cut(s) 121
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.