RchiOBHm_Chr3g0470871

Bifunctional monodehydroascorbate reductase and carbonic anhydrase nectarin-3-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
3
OG0001343
Physical Location & Seq
Reverse (-)
16808134 .. 16809087
954 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ43663

Sequence Viewer

Length: 672 bp
ATGGAGTATAATTATAGGGAAGGGAGCCCCAAGGGACCAGAGCACTGGGGAGAGCTCAACGAAGAAATGGAGAAATGCAAAGATGGAAAATTACAATCGCCAATAGATTTGTTCGATTCGAATGCTAGAAAAGCAATCTCAAGAGTAGATGATCTTAACATGAGCTATAACCCTTCAAAAGCAACTATGAAGCTTGAACACCATGCTATTTCGATTCAATGGGAGGGTGATGCTGGATCAATTCAAATAAATGGCACTGAATACTTGCTCAAAAGATGTCATTGGCACAGATCCTCTGAACATTTCATTAATGGCAGAAGGTTTCTCTTTCTCTTTGCTTCTCTGTTTGTAAAATCCAAAATACTTCATATATGTCTAAACAGAGTTTTTTTTATCAGTTATGATTTGGAATTGCACATGGTTCACCAAAGCCAGAACAACGATGTGGCTGTAGTCGCATTCCTATACCAAAGTGGCAATCCCGATCCTTTCCTGTCAAAGATAAGCAACGATGTATCCTCCCTAAGTGTTACAAAGGAGGCACGCTTGGGAGTGATTCATCCAAGTTTAATAAAATGGCCAAGCTCGCGATTTTATAGATATATAGGCTCACTCACGGCCCCTCCTTGCACTGAAGGAGTTATTTGGAGCGTAAGCGAAGAGGTACAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00910 map00910
Pfam Domains
Protein Families
Carb_anhydrase

Protein Analysis

223

Amino Acids

25.57

Weight (kDa)

6.55

Isoelectric Point (pI)

47.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Carb_anhydrase PF00194 11 - 108 3.5e-09 Eukaryotic-type carbonic anhydrase
Carb_anhydrase PF00194 133 - 221 9.8e-13 Eukaryotic-type carbonic anhydrase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 589
AclWI GGATC 3 cut(s) 244, 285, 479
AcoI YGGCCR 1 cut(s) 578
AcuI CTGAAG 1 cut(s) 654
AfaI GTAC 1 cut(s) 666
AgsI TTSAA 4 cut(s) 177, 197, 218, 245
AluBI AGCT 4 cut(s) 55, 165, 193, 585
AluI AGCT 4 cut(s) 55, 165, 193, 585
Alw21I GWGCWC 2 cut(s) 45, 57
AlwI GGATC 3 cut(s) 244, 285, 479
AoxI GGCC 2 cut(s) 578, 618
AseI ATTAAT 1 cut(s) 309
AspS9I GGNCC 2 cut(s) 35, 619
AsuHPI GGTGA 2 cut(s) 239, 416
AsuII TTCGAA 1 cut(s) 119
AvaII GGWCC 1 cut(s) 35
BalI TGGCCA 1 cut(s) 580
BanII GRGCYC 2 cut(s) 29, 57
Bbv12I GWGCWC 2 cut(s) 45, 57
BccI CCATC 1 cut(s) 77
BceAI ACGGC 1 cut(s) 633
BcgI CGANNNNNNTGC 2 cut(s) 104, 138
BciVI GTATCC 1 cut(s) 526
BfaI CTAG 1 cut(s) 126
BfmI CTRYAG 1 cut(s) 450
BfuI GTATCC 1 cut(s) 526
Bme18I GGWCC 1 cut(s) 35
BmgT120I GGNCC 2 cut(s) 35, 619
BmiI GGNNCC 3 cut(s) 26, 36, 621
BmrI ACTGGG 1 cut(s) 55
BmsI GCATC 1 cut(s) 220
BmuI ACTGGG 1 cut(s) 55
Bpu14I TTCGAA 1 cut(s) 119
BpuEI CTTGAG 1 cut(s) 124
BsaJI CCNNGG 1 cut(s) 30
BsaXI ACNNNNNCTCC 2 cut(s) 607, 637
Bse1I ACTGG 1 cut(s) 50
BseDI CCNNGG 1 cut(s) 30
BseGI GGATG 1 cut(s) 559
BseNI ACTGG 1 cut(s) 50
Bsh1236I CGCG 1 cut(s) 589
BshFI GGCC 2 cut(s) 580, 620
BsiHKAI GWGCWC 2 cut(s) 45, 57
BslFI GGGAC 1 cut(s) 48
BsmFI GGGAC 1 cut(s) 48
BsmI GAATGC 2 cut(s) 127, 458
BsnI GGCC 2 cut(s) 580, 620
Bsp119I TTCGAA 1 cut(s) 119
Bsp1286I GDGCHC 3 cut(s) 29, 45, 57
Bsp143I GATC 4 cut(s) 151, 236, 290, 484
Bsp68I TCGCGA 1 cut(s) 589
BspANI GGCC 2 cut(s) 580, 620
BspFNI CGCG 1 cut(s) 589
BspLI GGNNCC 3 cut(s) 26, 36, 621
BspPI GGATC 3 cut(s) 244, 285, 479
BspT104I TTCGAA 1 cut(s) 119
BsrI ACTGG 1 cut(s) 50
BssECI CCNNGG 1 cut(s) 30
BssMI GATC 4 cut(s) 151, 236, 290, 484
BssT1I CCWWGG 1 cut(s) 30
Bst6I CTCTTC 1 cut(s) 654
BstBI TTCGAA 1 cut(s) 119
BstC8I GCNNGC 2 cut(s) 544, 587
BstDEI CTNAG 1 cut(s) 524
BstF5I GGATG 1 cut(s) 559
BstFNI CGCG 1 cut(s) 589
BstKTI GATC 4 cut(s) 154, 239, 293, 487
BstMBI GATC 4 cut(s) 151, 236, 290, 484
BstMWI GCNNNNNNNGC 3 cut(s) 131, 455, 586
BstSFI CTRYAG 1 cut(s) 450
BstUI CGCG 1 cut(s) 589
BstX2I RGATCY 1 cut(s) 290
BstXI CCANNNNNNTGG 1 cut(s) 45
BstYI RGATCY 1 cut(s) 290
BsuI GTATCC 1 cut(s) 526
BsuRI GGCC 2 cut(s) 580, 620
BtsCI GGATG 1 cut(s) 559
BtsIMutI CAGTG 3 cut(s) 43, 255, 630
BtuMI TCGCGA 1 cut(s) 589
Cac8I GCNNGC 2 cut(s) 544, 587
Cfr13I GGNCC 2 cut(s) 35, 619
Csp6I GTAC 1 cut(s) 665
CviAII CATG 3 cut(s) 160, 203, 418
CviQI GTAC 1 cut(s) 665
DdeI CTNAG 1 cut(s) 524
DpnI GATC 4 cut(s) 153, 238, 292, 486
DpnII GATC 4 cut(s) 151, 236, 290, 484
EaeI YGGCCR 1 cut(s) 578
Eam1104I CTCTTC 1 cut(s) 654
EarI CTCTTC 1 cut(s) 654
Ecl136II GAGCTC 1 cut(s) 55
Eco130I CCWWGG 1 cut(s) 30
Eco24I GRGCYC 2 cut(s) 29, 57
Eco47I GGWCC 1 cut(s) 35
Eco53kI GAGCTC 1 cut(s) 55
Eco57I CTGAAG 1 cut(s) 654
EcoICRI GAGCTC 1 cut(s) 55
EcoT14I CCWWGG 1 cut(s) 30
EcoT38I GRGCYC 2 cut(s) 29, 57
ErhI CCWWGG 1 cut(s) 30
FaeI CATG 3 cut(s) 163, 206, 421
FaqI GGGAC 1 cut(s) 48
FatI CATG 3 cut(s) 159, 202, 417
FokI GGATG 1 cut(s) 546
FriOI GRGCYC 2 cut(s) 29, 57
FspBI CTAG 1 cut(s) 126
HaeIII GGCC 2 cut(s) 580, 620
Hin1II CATG 3 cut(s) 163, 206, 421
HindIII AAGCTT 1 cut(s) 191
HinfI GANTC 3 cut(s) 116, 214, 556
HphI GGTGA 2 cut(s) 239, 416
Hpy166II GTNNAC 1 cut(s) 424
Hpy188I TCNGA 1 cut(s) 298
Hpy188III TCNNGA 3 cut(s) 141, 482, 588
Hpy8I GTNNAC 1 cut(s) 424
HpyAV CCTTC 4 cut(s) 14, 183, 312, 629
HpyCH4V TGCA 3 cut(s) 78, 415, 630
HpyF10VI GCNNNNNNNGC 3 cut(s) 131, 455, 586
HpyF3I CTNAG 1 cut(s) 524
Hsp92II CATG 3 cut(s) 163, 206, 421
Kzo9I GATC 4 cut(s) 151, 236, 290, 484
LmnI GCTCC 2 cut(s) 24, 648
LpnPI CCDG 5 cut(s) 31, 51, 219, 446, 506
LweI GCATC 1 cut(s) 220
MaeI CTAG 1 cut(s) 126
MaeIII GTNAC 1 cut(s) 529
MalI GATC 4 cut(s) 153, 238, 292, 486
MboI GATC 4 cut(s) 151, 236, 290, 484
MboII GAAGA 2 cut(s) 74, 671
MflI RGATCY 1 cut(s) 290
MhlI GDGCHC 3 cut(s) 29, 45, 57
MlsI TGGCCA 1 cut(s) 580
MluCI AATT 4 cut(s) 10, 89, 240, 410
MluNI TGGCCA 1 cut(s) 580
MnlI CCTC 6 cut(s) 217, 304, 529, 532, 633, 655
Mox20I TGGCCA 1 cut(s) 580
MscI TGGCCA 1 cut(s) 580
MseI TTAA 3 cut(s) 156, 309, 569
Msp20I TGGCCA 1 cut(s) 580
Mva1269I GAATGC 2 cut(s) 127, 458
MvnI CGCG 1 cut(s) 589
MwoI GCNNNNNNNGC 3 cut(s) 131, 455, 586
NdeII GATC 4 cut(s) 151, 236, 290, 484
NlaIII CATG 3 cut(s) 163, 206, 421
NlaIV GGNNCC 3 cut(s) 26, 36, 621
NruI TCGCGA 1 cut(s) 589
NspV TTCGAA 1 cut(s) 119
PctI GAATGC 2 cut(s) 127, 458
PfeI GAWTC 3 cut(s) 116, 214, 556
PshBI ATTAAT 1 cut(s) 309
Psp124BI GAGCTC 1 cut(s) 57
PspN4I GGNNCC 3 cut(s) 26, 36, 621
PspPI GGNCC 2 cut(s) 35, 619
PsuI RGATCY 1 cut(s) 290
RruI TCGCGA 1 cut(s) 589
RsaI GTAC 1 cut(s) 666
RsaNI GTAC 1 cut(s) 665
SacI GAGCTC 1 cut(s) 57
SaqAI TTAA 3 cut(s) 156, 309, 569
Sau3AI GATC 4 cut(s) 151, 236, 290, 484
Sau96I GGNCC 2 cut(s) 35, 619
SduI GDGCHC 3 cut(s) 29, 45, 57
SetI ASST 6 cut(s) 57, 167, 195, 323, 587, 666
SfaNI GCATC 1 cut(s) 220
SfcI CTRYAG 1 cut(s) 450
SfuI TTCGAA 1 cut(s) 119
SinI GGWCC 1 cut(s) 35
SmlI CTYRAG 1 cut(s) 139
SmoI CTYRAG 1 cut(s) 139
Sse9I AATT 4 cut(s) 10, 89, 240, 410
SspMI CTAG 1 cut(s) 126
SstI GAGCTC 1 cut(s) 57
StyI CCWWGG 1 cut(s) 30
TaqI TCGA 3 cut(s) 114, 119, 212
TasI AATT 4 cut(s) 10, 89, 240, 410
TfiI GAWTC 3 cut(s) 116, 214, 556
Tru1I TTAA 3 cut(s) 156, 309, 569
Tru9I TTAA 3 cut(s) 156, 309, 569
TscAI CASTG 3 cut(s) 50, 262, 637
TspDTI ATGAA 4 cut(s) 203, 295, 356, 548
TspRI CASTG 3 cut(s) 50, 262, 637
VpaK11BI GGWCC 1 cut(s) 35
VspI ATTAAT 1 cut(s) 309
XspI CTAG 1 cut(s) 126
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.