Rh3AG161300

Bifunctional monodehydroascorbate reductase and carbonic anhydrase nectarin-3-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3A
OG0001343
Physical Location & Seq
Forward (+)
14836541 .. 14839513
2973 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3AG161300.1

Sequence Viewer

Length: 834 bp
ATGATTAGATCAATGAAAACTCCTCTTCAGTTCTTTGCTTTCACTCTTTTTCTTCTGTACTGGCAACCAATCAAACCCATCAGAGCTGGCCATGGTGTGGAAGAAATAGGGTATAATTATAGGAAAGGGAGCAAGAAGGGACCAGAGCACTGGGGAGACCTTAAGAAAGAATGGAAGGCATGCAAAGATGGAAAATTGCAATCGCCAATAAATTTGTCGGATAAGCACGCTAGAAAAGCAATCTCAAGAGTATATGATCTTAAAATGAGCTATAAGCCTTCAAATGCAACCATGAAGAATGAAGGTCATGCTATTGCGATTGAATGGGAGGGTGATGCAGGATCAATTCAGATCAATGGCACTGATTTTTTCCTCAAACAATGCCATTGGCACAGATCCTCTGAACATTTCATTAATGGCAGAAGTTATGATTTGGAATTGCACATGGTTCACCACAGCCGGAAGAACAACGTAGCTGTAGTCGCATTCCTCTACAAAATTGGTAGACGTAGTCCTTTTTTCTCAAAGATGAACAATGACGTATTGTCTCTAACTGGTATAGAGAAGGAGATACACTTGGGAGTGATTGATCCAAGATTGATAAGATGGCCAAGCTCACAATTTTACAGATATATGGGCTCACTCACAACCCCTCCTTGCACAGGTGGAGTTATTTGGAGCGTAAACAAAAAGGTACTTTCTGTTACAAAAGAACAACTGAGGTTACTTGAACGAGTCGTCTATGGTCATGCAAAGATGAATGCTAGACCATTACAACGTCTTAATGGCCGGGATATCACTCTCTTTGGTCCTCGGTGGGGATTGCGTAACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00910 map00910
Pfam Domains
Protein Families
Carb_anhydrase

Protein Analysis

277

Amino Acids

32.01

Weight (kDa)

10.0

Isoelectric Point (pI)

49.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Carb_anhydrase PF00194 46 - 267 1.1e-39 Eukaryotic-type carbonic anhydrase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 97
AccI GTMKAC 1 cut(s) 505
AclWI GGATC 3 cut(s) 349, 390, 584
AcoI YGGCCR 3 cut(s) 88, 608, 787
AcsI RAATTY 1 cut(s) 211
AcuI CTGAAG 1 cut(s) 11
AfaI GTAC 2 cut(s) 59, 696
AfiI CCNNNNNNNGG 3 cut(s) 97, 662, 818
AflII CTTAAG 1 cut(s) 161
AgsI TTSAA 3 cut(s) 282, 323, 731
AhdI GACNNNNNGTC 1 cut(s) 544
AluBI AGCT 4 cut(s) 86, 270, 476, 615
AluI AGCT 4 cut(s) 86, 270, 476, 615
Alw21I GWGCWC 1 cut(s) 150
Alw26I GTCTC 2 cut(s) 150, 552
AlwI GGATC 3 cut(s) 349, 390, 584
AoxI GGCC 3 cut(s) 88, 608, 787
ApoI RAATTY 1 cut(s) 211
AseI ATTAAT 1 cut(s) 414
AspS9I GGNCC 2 cut(s) 140, 809
AsuC2I CCSGG 1 cut(s) 791
AsuHPI GGTGA 2 cut(s) 344, 443
AvaII GGWCC 2 cut(s) 140, 809
BalI TGGCCA 2 cut(s) 90, 610
BanII GRGCYC 1 cut(s) 641
Bbv12I GWGCWC 1 cut(s) 150
BccI CCATC 3 cut(s) 86, 182, 600
BcnI CCSGG 1 cut(s) 791
BcoDI GTCTC 2 cut(s) 150, 552
BfaI CTAG 2 cut(s) 231, 765
BfmI CTRYAG 1 cut(s) 477
BfrI CTTAAG 1 cut(s) 161
Bme1390I CCNGG 1 cut(s) 791
Bme18I GGWCC 2 cut(s) 140, 809
BmeRI GACNNNNNGTC 1 cut(s) 544
BmgT120I GGNCC 2 cut(s) 140, 809
BmiI GGNNCC 1 cut(s) 141
BmrFI CCNGG 1 cut(s) 791
BmrI ACTGGG 1 cut(s) 160
BmsI GCATC 1 cut(s) 325
BmuI ACTGGG 1 cut(s) 160
BpuEI CTTGAG 1 cut(s) 229
BpuMI CCSGG 1 cut(s) 791
BsaI GGTCTC 1 cut(s) 150
BsaJI CCNNGG 2 cut(s) 91, 812
BsaXI ACNNNNNCTCC 2 cut(s) 637, 667
Bsc4I CCNNNNNNNGG 3 cut(s) 97, 662, 818
Bse1I ACTGG 3 cut(s) 65, 155, 559
BseDI CCNNGG 2 cut(s) 91, 812
BseLI CCNNNNNNNGG 3 cut(s) 97, 662, 818
BseMII CTCAG 1 cut(s) 710
BseNI ACTGG 3 cut(s) 65, 155, 559
BseRI GAGGAG 1 cut(s) 12
BshFI GGCC 3 cut(s) 90, 610, 789
BsiHKAI GWGCWC 1 cut(s) 150
BsiSI CCGG 2 cut(s) 460, 790
BslFI GGGAC 1 cut(s) 153
BslI CCNNNNNNNGG 3 cut(s) 97, 662, 818
BsmAI GTCTC 2 cut(s) 150, 552
BsmFI GGGAC 1 cut(s) 153
BsmI GAATGC 2 cut(s) 485, 766
BsnI GGCC 3 cut(s) 90, 610, 789
Bso31I GGTCTC 1 cut(s) 150
Bsp1286I GDGCHC 2 cut(s) 150, 641
Bsp143I GATC 6 cut(s) 8, 256, 341, 351, 395, 589
Bsp19I CCATGG 1 cut(s) 91
BspANI GGCC 3 cut(s) 90, 610, 789
BspCNI CTCAG 1 cut(s) 711
BspLI GGNNCC 1 cut(s) 141
BspPI GGATC 3 cut(s) 349, 390, 584
BspTI CTTAAG 1 cut(s) 161
BspTNI GGTCTC 1 cut(s) 150
BsrI ACTGG 3 cut(s) 65, 155, 559
BssECI CCNNGG 2 cut(s) 91, 812
BssMI GATC 6 cut(s) 8, 256, 341, 351, 395, 589
BssT1I CCWWGG 1 cut(s) 91
Bst6I CTCTTC 1 cut(s) 30
BstAFI CTTAAG 1 cut(s) 161
BstC8I GCNNGC 3 cut(s) 88, 181, 228
BstDEI CTNAG 1 cut(s) 719
BstDSI CCRYGG 1 cut(s) 91
BstENI CCTNNNNNAGG 1 cut(s) 660
BstKTI GATC 6 cut(s) 11, 259, 344, 354, 398, 592
BstMAI GTCTC 2 cut(s) 150, 552
BstMBI GATC 6 cut(s) 8, 256, 341, 351, 395, 589
BstMWI GCNNNNNNNGC 2 cut(s) 236, 482
BstNSI RCATGY 1 cut(s) 183
BstSCI CCNGG 1 cut(s) 789
BstSFI CTRYAG 1 cut(s) 477
BstX2I RGATCY 1 cut(s) 395
BstXI CCANNNNNNTGG 1 cut(s) 150
BstYI RGATCY 1 cut(s) 395
BsuRI GGCC 3 cut(s) 90, 610, 789
BtgI CCRYGG 1 cut(s) 91
BtsIMutI CAGTG 2 cut(s) 148, 360
Cac8I GCNNGC 3 cut(s) 88, 181, 228
Cfr13I GGNCC 2 cut(s) 140, 809
Csp6I GTAC 2 cut(s) 58, 695
CviAII CATG 6 cut(s) 92, 180, 292, 308, 445, 749
CviQI GTAC 2 cut(s) 58, 695
DdeI CTNAG 1 cut(s) 719
DpnI GATC 6 cut(s) 10, 258, 343, 353, 397, 591
DpnII GATC 6 cut(s) 8, 256, 341, 351, 395, 589
DriI GACNNNNNGTC 1 cut(s) 544
EaeI YGGCCR 3 cut(s) 88, 608, 787
Eam1104I CTCTTC 1 cut(s) 30
Eam1105I GACNNNNNGTC 1 cut(s) 544
EarI CTCTTC 1 cut(s) 30
Eco130I CCWWGG 1 cut(s) 91
Eco24I GRGCYC 1 cut(s) 641
Eco31I GGTCTC 1 cut(s) 150
Eco32I GATATC 1 cut(s) 796
Eco47I GGWCC 2 cut(s) 140, 809
Eco57I CTGAAG 1 cut(s) 11
EcoNI CCTNNNNNAGG 1 cut(s) 660
EcoRV GATATC 1 cut(s) 796
EcoT14I CCWWGG 1 cut(s) 91
EcoT38I GRGCYC 1 cut(s) 641
ErhI CCWWGG 1 cut(s) 91
FaeI CATG 6 cut(s) 95, 183, 295, 311, 448, 752
FaqI GGGAC 1 cut(s) 153
FatI CATG 6 cut(s) 91, 179, 291, 307, 444, 748
FblI GTMKAC 1 cut(s) 505
FriOI GRGCYC 1 cut(s) 641
FspBI CTAG 2 cut(s) 231, 765
HaeIII GGCC 3 cut(s) 90, 610, 789
HapII CCGG 2 cut(s) 460, 790
Hin1II CATG 6 cut(s) 95, 183, 295, 311, 448, 752
HinfI GANTC 1 cut(s) 735
HpaII CCGG 2 cut(s) 460, 790
HphI GGTGA 2 cut(s) 344, 443
Hpy166II GTNNAC 3 cut(s) 451, 506, 685
Hpy188I TCNGA 4 cut(s) 83, 220, 351, 403
Hpy188III TCNNGA 1 cut(s) 246
Hpy8I GTNNAC 3 cut(s) 451, 506, 685
HpyAV CCTTC 5 cut(s) 130, 169, 288, 296, 559
HpyCH4IV ACGT 4 cut(s) 471, 508, 540, 778
HpyCH4V TGCA 7 cut(s) 183, 199, 287, 338, 442, 660, 752
HpyF10VI GCNNNNNNNGC 2 cut(s) 236, 482
HpyF3I CTNAG 1 cut(s) 719
HpySE526I ACGT 4 cut(s) 471, 508, 540, 778
Hsp92II CATG 6 cut(s) 95, 183, 295, 311, 448, 752
Kzo9I GATC 6 cut(s) 8, 256, 341, 351, 395, 589
LmnI GCTCC 2 cut(s) 129, 678
LpnPI CCDG 9 cut(s) 46, 72, 136, 156, 324, 473, 540, 648, 803
LweI GCATC 1 cut(s) 325
MaeI CTAG 2 cut(s) 231, 765
MaeII ACGT 4 cut(s) 471, 508, 540, 778
MaeIII GTNAC 3 cut(s) 703, 723, 827
MalI GATC 6 cut(s) 10, 258, 343, 353, 397, 591
MboI GATC 6 cut(s) 8, 256, 341, 351, 395, 589
MboII GAAGA 5 cut(s) 17, 44, 113, 307, 475
MflI RGATCY 1 cut(s) 395
MhlI GDGCHC 2 cut(s) 150, 641
MlsI TGGCCA 2 cut(s) 90, 610
MluCI AATT 7 cut(s) 115, 194, 211, 345, 437, 498, 620
MluNI TGGCCA 2 cut(s) 90, 610
MlyI GAGTC 1 cut(s) 744
MmeI TCCRAC 1 cut(s) 198
MnlI CCTC 8 cut(s) 33, 322, 383, 409, 500, 663, 714, 822
Mox20I TGGCCA 2 cut(s) 90, 610
MscI TGGCCA 2 cut(s) 90, 610
MseI TTAA 4 cut(s) 162, 261, 414, 783
Msp20I TGGCCA 2 cut(s) 90, 610
MspCI CTTAAG 1 cut(s) 161
MspI CCGG 2 cut(s) 460, 790
MspR9I CCNGG 1 cut(s) 791
Mva1269I GAATGC 2 cut(s) 485, 766
MwoI GCNNNNNNNGC 2 cut(s) 236, 482
NciI CCSGG 1 cut(s) 791
NcoI CCATGG 1 cut(s) 91
NdeII GATC 6 cut(s) 8, 256, 341, 351, 395, 589
NlaIII CATG 6 cut(s) 95, 183, 295, 311, 448, 752
NlaIV GGNNCC 1 cut(s) 141
NspI RCATGY 1 cut(s) 183
PaeI GCATGC 1 cut(s) 183
PctI GAATGC 2 cut(s) 485, 766
PflFI GACNNNGTC 1 cut(s) 510
PflMI CCANNNNNTGG 1 cut(s) 97
PleI GAGTC 1 cut(s) 743
PpsI GAGTC 1 cut(s) 743
PshBI ATTAAT 1 cut(s) 414
PspN4I GGNNCC 1 cut(s) 141
PspPI GGNCC 2 cut(s) 140, 809
PsuI RGATCY 1 cut(s) 395
PsyI GACNNNGTC 1 cut(s) 510
RsaI GTAC 2 cut(s) 59, 696
RsaNI GTAC 2 cut(s) 58, 695
SaqAI TTAA 4 cut(s) 162, 261, 414, 783
Sau3AI GATC 6 cut(s) 8, 256, 341, 351, 395, 589
Sau96I GGNCC 2 cut(s) 140, 809
SchI GAGTC 1 cut(s) 744
ScrFI CCNGG 1 cut(s) 791
SduI GDGCHC 2 cut(s) 150, 641
SfaNI GCATC 1 cut(s) 325
SfcI CTRYAG 1 cut(s) 477
SinI GGWCC 2 cut(s) 140, 809
SmlI CTYRAG 2 cut(s) 161, 244
SmoI CTYRAG 2 cut(s) 161, 244
SphI GCATGC 1 cut(s) 183
Sse9I AATT 7 cut(s) 115, 194, 211, 345, 437, 498, 620
SspMI CTAG 2 cut(s) 231, 765
StyD4I CCNGG 1 cut(s) 789
StyI CCWWGG 1 cut(s) 91
TaiI ACGT 4 cut(s) 474, 511, 543, 781
TasI AATT 7 cut(s) 115, 194, 211, 345, 437, 498, 620
TatI WGTACW 1 cut(s) 57
Tru1I TTAA 4 cut(s) 162, 261, 414, 783
Tru9I TTAA 4 cut(s) 162, 261, 414, 783
TscAI CASTG 2 cut(s) 155, 367
TspDTI ATGAA 6 cut(s) 29, 308, 315, 400, 545, 773
TspRI CASTG 2 cut(s) 155, 367
Tth111I GACNNNGTC 1 cut(s) 510
Van91I CCANNNNNTGG 1 cut(s) 97
Vha464I CTTAAG 1 cut(s) 161
VpaK11BI GGWCC 2 cut(s) 140, 809
VspI ATTAAT 1 cut(s) 414
XagI CCTNNNNNAGG 1 cut(s) 660
XapI RAATTY 1 cut(s) 211
XceI RCATGY 1 cut(s) 183
XmiI GTMKAC 1 cut(s) 505
XspI CTAG 2 cut(s) 231, 765
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.