pycom12g10780

Bifunctional monodehydroascorbate reductase and carbonic anhydrase nectarin-3-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0001343
Physical Location & Seq
Reverse (-)
12820367 .. 12821955
1589 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom12g10780.6

Sequence Viewer

Length: 1017 bp
ATGGGGACTGGCACATCATGGAGAATTTGTGATTTGGTTTTCCATCCATTGGCAATTGCAACACATTTTGCTATAAAATCCAGGAGGAAAGAGATCTTGGGGGTATTACAATTTGTGACAATTGCCTCTCAAAACATGTTTGGATCAAAGCAAATCCACCTTGGCTTCATTGCATTCACACTGTTTCTTCTGTGCTGGGATGCATCACCCATTAGGGCTTCCAAATTTTCCAATGAGGAAGATCCCATAGAGTTTGCATACACTGAAGGGGACGAGAGAGGACCAGAACATTGGGGAGACCTTAAGGAAGAATGGAGCACATGTAAGGATGGAAAACTACAATCACCTATAGATTTGAGGGATACCATTGCAGACAAAGTTAACTCAACTTCGGATCATCTTAAAATCCATTACAAGCCTACCCAAGCCTTGATGAAGAACGAAGGTCATGCTATTTCGGTTGTGTGGGAGGGTTATGCCGGAGGAGTCACGATCAACGGCACAGAATACTTGCTCCGGCAATGCCATTGGCACGCCCCCTCCGAGCACACCATGAATGGCAAAACCTATGATGTGGAACTGCACATGGTTCACAAAGACACCAGCAACACTCGTGTAGCTGTGGTTGGATTCCTCTACAAAATTGGCAAACCCAATCCTTTCATCACACAGGTGCGAAAGGCAATACGGTCTATGATTCAAAAGCCAACGGAGGTCCACCTGGGAGTGGTCGATCCGAGGAAGATGAAAAAGGCTCAGAAGGGGAAGCAGGATGCGGCAAAGAGGAAGATGAAGAGGATGAGCTCAAAATTTTACAGATACATGGGATCGTTCACAACCCCTCCTTGCACCGAAGGAGTTACTTGGACCATAAACAAACAGGTACATACAGTTTCACGATCACAGGTGGCGTTGCTTCAAAAAGCCGTTTACAAGTATGCAGAGATGAATTCAAGACCAGTACAACCTCTAAATGACCGGGAGGTCAAGCTCCACGGCTCGTCGTTCAGCAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00910 map00910
Pfam Domains
Protein Families
Carb_anhydrase

Protein Analysis

339

Amino Acids

38.4

Weight (kDa)

9.52

Isoelectric Point (pI)

42.51

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 983
AccB7I CCANNNNNTGG 1 cut(s) 49
AciI CCGC 1 cut(s) 776
AclWI GGATC 5 cut(s) 151, 236, 402, 728, 835
AcsI RAATTY 4 cut(s) 24, 224, 809, 949
AcuI CTGAAG 1 cut(s) 285
AfaI GTAC 2 cut(s) 885, 963
AfiI CCNNNNNNNGG 2 cut(s) 49, 727
AflII CTTAAG 1 cut(s) 302
AflIII ACRYGT 2 cut(s) 135, 320
AgsI TTSAA 3 cut(s) 701, 920, 954
AjnI CCWGG 2 cut(s) 80, 720
AjuI GAANNNNNNNTTGG 2 cut(s) 80, 112
AleI CACNNNNGTG 1 cut(s) 671
AluBI AGCT 3 cut(s) 620, 804, 991
AluI AGCT 3 cut(s) 620, 804, 991
Alw21I GWGCWC 3 cut(s) 320, 549, 806
Alw26I GTCTC 1 cut(s) 291
AlwI GGATC 5 cut(s) 151, 236, 402, 728, 835
ApoI RAATTY 4 cut(s) 24, 224, 809, 949
AspS9I GGNCC 3 cut(s) 281, 715, 867
AsuC2I CCSGG 1 cut(s) 980
AsuHPI GGTGA 2 cut(s) 198, 336
AvaII GGWCC 3 cut(s) 281, 715, 867
BanII GRGCYC 1 cut(s) 806
BauI CACGAG 1 cut(s) 612
Bbv12I GWGCWC 3 cut(s) 320, 549, 806
BccI CCATC 2 cut(s) 51, 323
BceAI ACGGC 3 cut(s) 514, 911, 1012
BcgI CGANNNNNNTGC 2 cut(s) 431, 465
BciT130I CCWGG 2 cut(s) 82, 722
BciVI GTATCC 1 cut(s) 355
BcnI CCSGG 1 cut(s) 980
BcoDI GTCTC 1 cut(s) 291
BfmI CTRYAG 1 cut(s) 348
BfrI CTTAAG 1 cut(s) 302
BfuI GTATCC 1 cut(s) 355
BglII AGATCT 1 cut(s) 93
BisI GCNGC 1 cut(s) 777
BlsI GCNGC 1 cut(s) 778
Bme1390I CCNGG 3 cut(s) 82, 722, 980
Bme18I GGWCC 3 cut(s) 281, 715, 867
BmgT120I GGNCC 3 cut(s) 281, 715, 867
BmrFI CCNGG 3 cut(s) 82, 722, 980
BmsI GCATC 3 cut(s) 190, 212, 763
BpuMI CCSGG 1 cut(s) 980
BsaI GGTCTC 1 cut(s) 291
BsaJI CCNNGG 4 cut(s) 160, 721, 737, 994
BsaXI ACNNNNNCTCC 8 cut(s) 13, 43, 307, 337, 524, 554, 826, 856
Bsc4I CCNNNNNNNGG 2 cut(s) 49, 727
Bse1I ACTGG 2 cut(s) 13, 959
Bse3DI GCAATG 3 cut(s) 168, 366, 527
BseBI CCWGG 2 cut(s) 82, 722
BseDI CCNNGG 4 cut(s) 160, 721, 737, 994
BseGI GGATG 5 cut(s) 43, 205, 334, 778, 804
BseLI CCNNNNNNNGG 2 cut(s) 49, 727
BseMI GCAATG 3 cut(s) 168, 366, 527
BseMII CTCAG 1 cut(s) 770
BseNI ACTGG 2 cut(s) 13, 959
BseRI GAGGAG 1 cut(s) 498
BseYI CCCAGC 1 cut(s) 195
BsgI GTGCAG 1 cut(s) 566
BsiHKAI GWGCWC 3 cut(s) 320, 549, 806
BsiSI CCGG 3 cut(s) 480, 517, 979
BslFI GGGAC 2 cut(s) 19, 284
BslI CCNNNNNNNGG 2 cut(s) 49, 727
BsmAI GTCTC 1 cut(s) 291
BsmFI GGGAC 2 cut(s) 19, 284
BsmI GAATGC 1 cut(s) 173
Bso31I GGTCTC 1 cut(s) 291
Bsp1286I GDGCHC 3 cut(s) 320, 549, 806
Bsp143I GATC 8 cut(s) 93, 143, 241, 394, 492, 733, 827, 899
BspACI CCGC 1 cut(s) 776
BspCNI CTCAG 1 cut(s) 769
BspPI GGATC 5 cut(s) 151, 236, 402, 728, 835
BspTI CTTAAG 1 cut(s) 302
BspTNI GGTCTC 1 cut(s) 291
BsrDI GCAATG 3 cut(s) 168, 366, 527
BsrI ACTGG 2 cut(s) 13, 959
BssECI CCNNGG 4 cut(s) 160, 721, 737, 994
BssMI GATC 8 cut(s) 93, 143, 241, 394, 492, 733, 827, 899
BssSI CACGAG 1 cut(s) 612
BssT1I CCWWGG 1 cut(s) 160
Bst2BI CACGAG 1 cut(s) 612
Bst2UI CCWGG 2 cut(s) 82, 722
Bst4CI ACNGT 3 cut(s) 183, 690, 892
Bst6I CTCTTC 1 cut(s) 788
BstAFI CTTAAG 1 cut(s) 302
BstC8I GCNNGC 1 cut(s) 534
BstDEI CTNAG 1 cut(s) 756
BstDSI CCRYGG 1 cut(s) 994
BstF5I GGATG 5 cut(s) 43, 205, 334, 778, 804
BstKTI GATC 8 cut(s) 96, 146, 244, 397, 495, 736, 830, 902
BstMAI GTCTC 1 cut(s) 291
BstMBI GATC 8 cut(s) 93, 143, 241, 394, 492, 733, 827, 899
BstNI CCWGG 2 cut(s) 82, 722
BstNSI RCATGY 2 cut(s) 139, 324
BstSCI CCNGG 3 cut(s) 80, 720, 978
BstSFI CTRYAG 1 cut(s) 348
BstX2I RGATCY 2 cut(s) 93, 241
BstXI CCANNNNNNTGG 1 cut(s) 291
BstYI RGATCY 2 cut(s) 93, 241
BsuI GTATCC 1 cut(s) 355
BtgI CCRYGG 1 cut(s) 994
BtsCI GGATG 5 cut(s) 43, 205, 334, 778, 804
BtsIMutI CAGTG 2 cut(s) 179, 261
Cac8I GCNNGC 1 cut(s) 534
Cfr13I GGNCC 3 cut(s) 281, 715, 867
Csp6I GTAC 2 cut(s) 884, 962
CviAII CATG 7 cut(s) 18, 136, 321, 449, 553, 586, 823
CviQI GTAC 2 cut(s) 884, 962
DdeI CTNAG 1 cut(s) 756
DpnI GATC 8 cut(s) 95, 145, 243, 396, 494, 735, 829, 901
DpnII GATC 8 cut(s) 93, 143, 241, 394, 492, 733, 827, 899
DrdI GACNNNNNNGTC 1 cut(s) 983
DseDI GACNNNNNNGTC 1 cut(s) 983
Eam1104I CTCTTC 1 cut(s) 788
EarI CTCTTC 1 cut(s) 788
Ecl136II GAGCTC 1 cut(s) 804
Eco130I CCWWGG 1 cut(s) 160
Eco24I GRGCYC 1 cut(s) 806
Eco31I GGTCTC 1 cut(s) 291
Eco47I GGWCC 3 cut(s) 281, 715, 867
Eco53kI GAGCTC 1 cut(s) 804
Eco57I CTGAAG 1 cut(s) 285
EcoICRI GAGCTC 1 cut(s) 804
EcoRI GAATTC 1 cut(s) 949
EcoRII CCWGG 2 cut(s) 80, 720
EcoT14I CCWWGG 1 cut(s) 160
EcoT22I ATGCAT 1 cut(s) 205
EcoT38I GRGCYC 1 cut(s) 806
ErhI CCWWGG 1 cut(s) 160
FaeI CATG 7 cut(s) 21, 139, 324, 452, 556, 589, 826
FaqI GGGAC 2 cut(s) 19, 284
FatI CATG 7 cut(s) 17, 135, 320, 448, 552, 585, 822
Fnu4HI GCNGC 1 cut(s) 777
FokI GGATG 5 cut(s) 30, 212, 341, 785, 811
FriOI GRGCYC 1 cut(s) 806
Fsp4HI GCNGC 1 cut(s) 777
GluI GCNGC 1 cut(s) 777
GsaI CCCAGC 1 cut(s) 199
HapII CCGG 3 cut(s) 480, 517, 979
Hin1II CATG 7 cut(s) 21, 139, 324, 452, 556, 589, 826
HincII GTYRAC 1 cut(s) 382
HindII GTYRAC 1 cut(s) 382
HinfI GANTC 3 cut(s) 486, 630, 697
HpaI GTTAAC 1 cut(s) 382
HpaII CCGG 3 cut(s) 480, 517, 979
HphI GGTGA 2 cut(s) 198, 336
Hpy166II GTNNAC 5 cut(s) 382, 592, 718, 834, 931
Hpy188I TCNGA 4 cut(s) 394, 544, 738, 759
Hpy188III TCNNGA 3 cut(s) 490, 897, 954
Hpy8I GTNNAC 5 cut(s) 382, 592, 718, 834, 931
Hpy99I CGWCG 1 cut(s) 1006
HpyAV CCTTC 4 cut(s) 260, 437, 754, 848
HpyCH4III ACNGT 3 cut(s) 183, 690, 892
HpyCH4V TGCA 8 cut(s) 59, 173, 203, 257, 371, 583, 849, 941
HpyF3I CTNAG 1 cut(s) 756
Hsp92II CATG 7 cut(s) 21, 139, 324, 452, 556, 589, 826
KspAI GTTAAC 1 cut(s) 382
Kzo9I GATC 8 cut(s) 93, 143, 241, 394, 492, 733, 827, 899
LmnI GCTCC 3 cut(s) 315, 519, 996
LweI GCATC 3 cut(s) 190, 212, 763
MaeIII GTNAC 3 cut(s) 115, 487, 859
MalI GATC 8 cut(s) 95, 145, 243, 396, 494, 735, 829, 901
MboI GATC 8 cut(s) 93, 143, 241, 394, 492, 733, 827, 899
MboII GAAGA 7 cut(s) 179, 251, 320, 448, 754, 799, 805
MfeI CAATTG 2 cut(s) 54, 120
MflI RGATCY 2 cut(s) 93, 241
MhlI GDGCHC 3 cut(s) 320, 549, 806
MluCI AATT 8 cut(s) 24, 54, 110, 120, 224, 642, 809, 949
MlyI GAGTC 1 cut(s) 495
MmeI TCCRAC 1 cut(s) 607
Mph1103I ATGCAT 1 cut(s) 205
MseI TTAA 3 cut(s) 303, 381, 402
MslI CAYNNNNRTG 1 cut(s) 671
MspCI CTTAAG 1 cut(s) 302
MspI CCGG 3 cut(s) 480, 517, 979
MspR9I CCNGG 3 cut(s) 82, 722, 980
MunI CAATTG 2 cut(s) 54, 120
Mva1269I GAATGC 1 cut(s) 173
MvaI CCWGG 2 cut(s) 82, 722
NciI CCSGG 1 cut(s) 980
NdeII GATC 8 cut(s) 93, 143, 241, 394, 492, 733, 827, 899
NlaIII CATG 7 cut(s) 21, 139, 324, 452, 556, 589, 826
NmuCI GTSAC 2 cut(s) 115, 487
NsiI ATGCAT 1 cut(s) 205
NspI RCATGY 2 cut(s) 139, 324
OliI CACNNNNGTG 1 cut(s) 671
PciI ACATGT 2 cut(s) 135, 320
PcsI WCGNNNNNNNCGW 1 cut(s) 540
PctI GAATGC 1 cut(s) 173
PfeI GAWTC 2 cut(s) 630, 697
PflMI CCANNNNNTGG 1 cut(s) 49
PfoI TCCNGGA 1 cut(s) 80
PkrI GCNGC 1 cut(s) 778
PleI GAGTC 1 cut(s) 494
PpsI GAGTC 1 cut(s) 494
PscI ACATGT 2 cut(s) 135, 320
Psp124BI GAGCTC 1 cut(s) 806
Psp6I CCWGG 2 cut(s) 80, 720
PspFI CCCAGC 1 cut(s) 195
PspGI CCWGG 2 cut(s) 80, 720
PspPI GGNCC 3 cut(s) 281, 715, 867
PsuI RGATCY 2 cut(s) 93, 241
RsaI GTAC 2 cut(s) 885, 963
RsaNI GTAC 2 cut(s) 884, 962
RseI CAYNNNNRTG 1 cut(s) 671
SacI GAGCTC 1 cut(s) 806
SaqAI TTAA 3 cut(s) 303, 381, 402
SatI GCNGC 1 cut(s) 777
Sau3AI GATC 8 cut(s) 93, 143, 241, 394, 492, 733, 827, 899
Sau96I GGNCC 3 cut(s) 281, 715, 867
SchI GAGTC 1 cut(s) 495
ScrFI CCNGG 3 cut(s) 82, 722, 980
SduI GDGCHC 3 cut(s) 320, 549, 806
SfaNI GCATC 3 cut(s) 190, 212, 763
SfcI CTRYAG 1 cut(s) 348
SinI GGWCC 3 cut(s) 281, 715, 867
SmiMI CAYNNNNRTG 1 cut(s) 671
SmlI CTYRAG 1 cut(s) 302
SmoI CTYRAG 1 cut(s) 302
Sse9I AATT 8 cut(s) 24, 54, 110, 120, 224, 642, 809, 949
SsiI CCGC 1 cut(s) 776
SstI GAGCTC 1 cut(s) 806
StyD4I CCNGG 3 cut(s) 80, 720, 978
StyI CCWWGG 1 cut(s) 160
TaaI ACNGT 3 cut(s) 183, 690, 892
TaqI TCGA 1 cut(s) 732
TasI AATT 8 cut(s) 24, 54, 110, 120, 224, 642, 809, 949
TatI WGTACW 1 cut(s) 961
TauI GCSGC 1 cut(s) 779
TfiI GAWTC 2 cut(s) 630, 697
Tru1I TTAA 3 cut(s) 303, 381, 402
Tru9I TTAA 3 cut(s) 303, 381, 402
TscAI CASTG 2 cut(s) 186, 268
TseFI GTSAC 2 cut(s) 115, 487
Tsp45I GTSAC 2 cut(s) 115, 487
TspDTI ATGAA 7 cut(s) 157, 449, 569, 652, 761, 806, 962
TspGWI ACGGA 1 cut(s) 725
TspRI CASTG 2 cut(s) 186, 268
Van91I CCANNNNNTGG 1 cut(s) 49
Vha464I CTTAAG 1 cut(s) 302
VpaK11BI GGWCC 3 cut(s) 281, 715, 867
XapI RAATTY 4 cut(s) 24, 224, 809, 949
XceI RCATGY 2 cut(s) 139, 324
Zsp2I ATGCAT 1 cut(s) 205
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.