RchiOBHm_Chr3g0470681

Bifunctional monodehydroascorbate reductase and carbonic anhydrase nectarin-3-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
3
OG0001343
Physical Location & Seq
Forward (+)
16644763 .. 16648087
3325 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ43645

Sequence Viewer

Length: 855 bp
ATGTTTGTATCAAAGGAATCCCTTCTTGGCTTCATTGTTTTTACCTTTTTCCTTCTTTGCTGGAACCCGACACCCATCAGAGCAAACGACGATGGGGAAGTAGACTTTGATTATCGGGAAGACCACCCGAGAGGACCACAACACTGGGGATACCTTAAGAAAGAATGGAGCTTATGCAAGGATGGAAAAAGGCAATCACCAATAGAGATATGGGAAAAGAATGCCACCAAAGACATCACAGATTCAAAGGATCTTATAGTGAGTTATAAGCCTGCAAATGCAACTATCAAAAATGAAGAACATGCTATATCGGTTTTATGGGAGGGTGATGCTGGCTCAATTGAAATCAATGGCACAACCTACCTCCTCAAACAATGTCATTGGCACCACCCCTCTGAGCATGTCGTTAATGGCAGAAGGTTTGATCTGGAATTGCACATGGTTCACAAAACTCAGGACATGAGCAAAATAGCTGTAATCGGATTCCTCTACAACTTTGGTGAACCTAATTCTTTTCTCACAAAGGTGACAGGACACATCACTTCTTTGACTGATGTACAAAAGGGGATACCCTTGGGAGTGCTTGATCCAAGGAAGGTGAAAAAGGGTCAACTCCATTACAAGATTGCGAAGCCAGAAAAGGCTGGCTCTAAATTTTACAGATACATGGGCTCTCTCACAACCCCTCCTTGTGCTGAAGAGGTTATTTGGCACATAAACAAAAAGGTACAAACTGCTTCGATAGAACAAGTGAAGCTACTTCAAGATGCAGTTTTTGATTTTGCGGCGAACAACGCAAGACCATTACAACCTCTCAACGGCCGAGGAATCAAGCTCTATGGGGACTATCATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00910 map00910
Pfam Domains
Protein Families
Carb_anhydrase

Protein Analysis

284

Amino Acids

32.43

Weight (kDa)

8.21

Isoelectric Point (pI)

39.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Carb_anhydrase PF00194 45 - 278 1.2e-38 Eukaryotic-type carbonic anhydrase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 267
AccB1I GGYRCC 1 cut(s) 384
AccI GTMKAC 1 cut(s) 102
AciI CCGC 1 cut(s) 785
AclWI GGATC 2 cut(s) 258, 581
AcoI YGGCCR 1 cut(s) 820
AcsI RAATTY 1 cut(s) 653
AcuI CTGAAG 1 cut(s) 717
AfaI GTAC 2 cut(s) 558, 729
AfiI CCNNNNNNNGG 1 cut(s) 818
AflII CTTAAG 1 cut(s) 155
AgsI TTSAA 3 cut(s) 246, 344, 764
AjuI GAANNNNNNNTTGG 2 cut(s) 9, 41
AleI CACNNNNGTG 1 cut(s) 524
AluBI AGCT 4 cut(s) 171, 473, 757, 835
AluI AGCT 4 cut(s) 171, 473, 757, 835
AlwI GGATC 2 cut(s) 258, 581
Ama87I CYCGRG 1 cut(s) 127
AoxI GGCC 1 cut(s) 820
ApoI RAATTY 1 cut(s) 653
Asp700I GAANNNNTTC 1 cut(s) 21
AspS9I GGNCC 1 cut(s) 134
AsuHPI GGTGA 5 cut(s) 189, 338, 512, 538, 610
AvaI CYCGRG 1 cut(s) 127
AvaII GGWCC 1 cut(s) 134
BanI GGYRCC 1 cut(s) 384
BanII GRGCYC 1 cut(s) 674
BbsI GAAGAC 1 cut(s) 126
BccI CCATC 3 cut(s) 83, 86, 176
BceAI ACGGC 1 cut(s) 835
BciVI GTATCC 2 cut(s) 143, 561
BfrI CTTAAG 1 cut(s) 155
BfuI GTATCC 2 cut(s) 143, 561
BisI GCNGC 1 cut(s) 786
BlsI GCNGC 1 cut(s) 787
Bme18I GGWCC 1 cut(s) 134
BmeT110I CYCGRG 1 cut(s) 127
BmgT120I GGNCC 1 cut(s) 134
BmiI GGNNCC 2 cut(s) 65, 386
BmrI ACTGGG 1 cut(s) 154
BmsI GCATC 2 cut(s) 319, 757
BmuI ACTGGG 1 cut(s) 154
BpiI GAAGAC 1 cut(s) 126
BsaJI CCNNGG 3 cut(s) 573, 590, 823
BsaXI ACNNNNNCTCC 2 cut(s) 670, 700
Bsc4I CCNNNNNNNGG 1 cut(s) 818
Bse1I ACTGG 1 cut(s) 149
BseDI CCNNGG 3 cut(s) 573, 590, 823
BseGI GGATG 1 cut(s) 187
BseLI CCNNNNNNNGG 1 cut(s) 818
BseMII CTCAG 2 cut(s) 387, 467
BseNI ACTGG 1 cut(s) 149
BseRI GAGGAG 1 cut(s) 356
BseX3I CGGCCG 1 cut(s) 820
Bsh1285I CGRYCG 1 cut(s) 823
BshFI GGCC 1 cut(s) 822
BshNI GGYRCC 1 cut(s) 384
BsiEI CGRYCG 1 cut(s) 823
BsiHKCI CYCGRG 1 cut(s) 127
BslI CCNNNNNNNGG 1 cut(s) 818
BsmI GAATGC 1 cut(s) 226
BsnI GGCC 1 cut(s) 822
BsoBI CYCGRG 1 cut(s) 127
Bsp1286I GDGCHC 1 cut(s) 674
Bsp1407I TGTACA 1 cut(s) 556
Bsp143I GATC 3 cut(s) 250, 424, 586
BspACI CCGC 1 cut(s) 785
BspANI GGCC 1 cut(s) 822
BspCNI CTCAG 2 cut(s) 388, 466
BspLI GGNNCC 2 cut(s) 65, 386
BspPI GGATC 2 cut(s) 258, 581
BspT107I GGYRCC 1 cut(s) 384
BspTI CTTAAG 1 cut(s) 155
BsrGI TGTACA 1 cut(s) 556
BsrI ACTGG 1 cut(s) 149
BssECI CCNNGG 3 cut(s) 573, 590, 823
BssMI GATC 3 cut(s) 250, 424, 586
BssT1I CCWWGG 2 cut(s) 573, 590
Bst6I CTCTTC 1 cut(s) 693
BstAFI CTTAAG 1 cut(s) 155
BstAUI TGTACA 1 cut(s) 556
BstC8I GCNNGC 3 cut(s) 273, 334, 646
BstDEI CTNAG 2 cut(s) 396, 453
BstF5I GGATG 1 cut(s) 187
BstKTI GATC 3 cut(s) 253, 427, 589
BstMBI GATC 3 cut(s) 250, 424, 586
BstMCI CGRYCG 1 cut(s) 823
BstMWI GCNNNNNNNGC 1 cut(s) 794
BstNSI RCATGY 2 cut(s) 305, 404
BstV2I GAAGAC 1 cut(s) 126
BstX2I RGATCY 1 cut(s) 250
BstXI CCANNNNNNTGG 1 cut(s) 144
BstYI RGATCY 1 cut(s) 250
BstZI CGGCCG 1 cut(s) 820
BsuI GTATCC 2 cut(s) 143, 561
BsuRI GGCC 1 cut(s) 822
BtsCI GGATG 1 cut(s) 187
BtsIMutI CAGTG 1 cut(s) 142
Cac8I GCNNGC 3 cut(s) 273, 334, 646
Cfr13I GGNCC 1 cut(s) 134
Csp6I GTAC 2 cut(s) 557, 728
CviAII CATG 5 cut(s) 302, 401, 439, 460, 667
CviQI GTAC 2 cut(s) 557, 728
DdeI CTNAG 2 cut(s) 396, 453
DpnI GATC 3 cut(s) 252, 426, 588
DpnII GATC 3 cut(s) 250, 424, 586
EaeI YGGCCR 1 cut(s) 820
EagI CGGCCG 1 cut(s) 820
Eam1104I CTCTTC 1 cut(s) 693
EarI CTCTTC 1 cut(s) 693
EclXI CGGCCG 1 cut(s) 820
Eco130I CCWWGG 2 cut(s) 573, 590
Eco24I GRGCYC 1 cut(s) 674
Eco47I GGWCC 1 cut(s) 134
Eco52I CGGCCG 1 cut(s) 820
Eco57I CTGAAG 1 cut(s) 717
Eco88I CYCGRG 1 cut(s) 127
EcoT14I CCWWGG 2 cut(s) 573, 590
EcoT38I GRGCYC 1 cut(s) 674
ErhI CCWWGG 2 cut(s) 573, 590
FaeI CATG 5 cut(s) 305, 404, 442, 463, 670
FatI CATG 5 cut(s) 301, 400, 438, 459, 666
FblI GTMKAC 1 cut(s) 102
Fnu4HI GCNGC 1 cut(s) 786
FokI GGATG 1 cut(s) 194
FriOI GRGCYC 1 cut(s) 674
Fsp4HI GCNGC 1 cut(s) 786
GluI GCNGC 1 cut(s) 786
HaeIII GGCC 1 cut(s) 822
Hin1II CATG 5 cut(s) 305, 404, 442, 463, 670
HincII GTYRAC 1 cut(s) 611
HindII GTYRAC 1 cut(s) 611
HinfI GANTC 4 cut(s) 17, 242, 483, 828
HphI GGTGA 5 cut(s) 189, 338, 512, 538, 610
Hpy166II GTNNAC 4 cut(s) 103, 445, 503, 611
Hpy188I TCNGA 3 cut(s) 80, 397, 482
Hpy188III TCNNGA 4 cut(s) 116, 428, 455, 764
Hpy8I GTNNAC 4 cut(s) 103, 445, 503, 611
Hpy99I CGWCG 1 cut(s) 92
HpyAV CCTTC 4 cut(s) 32, 62, 411, 589
HpyCH4V TGCA 5 cut(s) 177, 275, 281, 436, 770
HpyF10VI GCNNNNNNNGC 1 cut(s) 794
HpyF3I CTNAG 2 cut(s) 396, 453
Hsp92II CATG 5 cut(s) 305, 404, 442, 463, 670
Kzo9I GATC 3 cut(s) 250, 424, 586
LmnI GCTCC 1 cut(s) 168
LpnPI CCDG 9 cut(s) 46, 130, 285, 318, 413, 440, 516, 630, 648
LweI GCATC 2 cut(s) 319, 757
MaeIII GTNAC 1 cut(s) 526
MalI GATC 3 cut(s) 252, 426, 588
MboI GATC 3 cut(s) 250, 424, 586
MboII GAAGA 3 cut(s) 131, 308, 710
MfeI CAATTG 1 cut(s) 339
MflI RGATCY 1 cut(s) 250
MhlI GDGCHC 1 cut(s) 674
MluCI AATT 4 cut(s) 339, 431, 508, 653
MroXI GAANNNNTTC 1 cut(s) 21
MseI TTAA 2 cut(s) 156, 408
MslI CAYNNNNRTG 1 cut(s) 524
MspCI CTTAAG 1 cut(s) 155
MunI CAATTG 1 cut(s) 339
Mva1269I GAATGC 1 cut(s) 226
MwoI GCNNNNNNNGC 1 cut(s) 794
NdeII GATC 3 cut(s) 250, 424, 586
NlaIII CATG 5 cut(s) 305, 404, 442, 463, 670
NlaIV GGNNCC 2 cut(s) 65, 386
NmeAIII GCCGAG 1 cut(s) 848
NmuCI GTSAC 1 cut(s) 526
NspI RCATGY 2 cut(s) 305, 404
OliI CACNNNNGTG 1 cut(s) 524
PctI GAATGC 1 cut(s) 226
PdmI GAANNNNTTC 1 cut(s) 21
PfeI GAWTC 4 cut(s) 17, 242, 483, 828
PkrI GCNGC 1 cut(s) 787
PsiI TTATAA 1 cut(s) 267
PspN4I GGNNCC 2 cut(s) 65, 386
PspPI GGNCC 1 cut(s) 134
PsuI RGATCY 1 cut(s) 250
RsaI GTAC 2 cut(s) 558, 729
RsaNI GTAC 2 cut(s) 557, 728
RseI CAYNNNNRTG 1 cut(s) 524
SaqAI TTAA 2 cut(s) 156, 408
SatI GCNGC 1 cut(s) 786
Sau3AI GATC 3 cut(s) 250, 424, 586
Sau96I GGNCC 1 cut(s) 134
SduI GDGCHC 1 cut(s) 674
SfaNI GCATC 2 cut(s) 319, 757
SinI GGWCC 1 cut(s) 134
SmiMI CAYNNNNRTG 1 cut(s) 524
SmlI CTYRAG 1 cut(s) 155
SmoI CTYRAG 1 cut(s) 155
Sse9I AATT 4 cut(s) 339, 431, 508, 653
SsiI CCGC 1 cut(s) 785
StyI CCWWGG 2 cut(s) 573, 590
TaqI TCGA 1 cut(s) 740
TasI AATT 4 cut(s) 339, 431, 508, 653
TatI WGTACW 1 cut(s) 556
TauI GCSGC 1 cut(s) 788
TfiI GAWTC 4 cut(s) 17, 242, 483, 828
Tru1I TTAA 2 cut(s) 156, 408
Tru9I TTAA 2 cut(s) 156, 408
TscAI CASTG 1 cut(s) 149
TseFI GTSAC 1 cut(s) 526
Tsp45I GTSAC 1 cut(s) 526
TspDTI ATGAA 2 cut(s) 22, 309
TspRI CASTG 1 cut(s) 149
Vha464I CTTAAG 1 cut(s) 155
VpaK11BI GGWCC 1 cut(s) 134
XapI RAATTY 1 cut(s) 653
XceI RCATGY 2 cut(s) 305, 404
XcmI CCANNNNNNNNNTGG 1 cut(s) 207
XmiI GTMKAC 1 cut(s) 102
XmnI GAANNNNTTC 1 cut(s) 21
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.